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Protein

tRNA pseudouridine synthase A

Gene

Pus1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:10094309). Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (PubMed:15327771). Isoforms 3 and 4 do not form pseudouridine when expressed in vitro (PubMed:11085940).3 Publications

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142NucleophileBy similarity1
Binding sitei197SubstrateBy similarity1

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
  • pseudouridine synthase activity Source: MGI
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: GO_Central
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • tRNA pseudouridine synthesis Source: MGI

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BRENDAi4.2.1.70 3474
5.4.99.B22 3474

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase A (EC:5.4.99.12)
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene namesi
Name:Pus1
ORF Names:MNCb-0873
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1929237 Pus1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000057518? – 423tRNA pseudouridine synthase A
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei411PhosphoserineBy similarity1
Modified residuei416PhosphoserineCombined sources1
Modified residuei422PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WU56
PeptideAtlasiQ9WU56
PRIDEiQ9WU56

PTM databases

iPTMnetiQ9WU56
PhosphoSitePlusiQ9WU56

Expressioni

Gene expression databases

BgeeiENSMUSG00000029507
CleanExiMM_PUS1
ExpressionAtlasiQ9WU56 baseline and differential
GenevisibleiQ9WU56 MM

Interactioni

Subunit structurei

Monomer (By similarity). Forms a complex with RARG and the SRA1 RNA in the nucleus (PubMed:15327771).By similarity1 Publication

Protein-protein interaction databases

BioGridi207924, 1 interactor
STRINGi10090.ENSMUSP00000083844

Structurei

3D structure databases

ProteinModelPortaliQ9WU56
SMRiQ9WU56
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2553 Eukaryota
COG0101 LUCA
GeneTreeiENSGT00510000047706
HOVERGENiHBG000489
InParanoidiQ9WU56
KOiK06173
PhylomeDBiQ9WU56

Family and domain databases

Gene3Di3.30.70.660, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00071 hisT_truA, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU56-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGFPRLWAAL LRNWGRWTAR PGPRVPGLPP MAGNKVPPAL ASHQPDRKGR
60 70 80 90 100
GGWVWEETEH PAKRVKGGED EEPPRKLPKR KIVLLMAYSG KGYHGMQRNL
110 120 130 140 150
GSSQFRTIED DLVSALVQAG CIPENHGTDM RKMSFQRCAR TDKGVSAAGQ
160 170 180 190 200
VVSLKVWLID DILDKINSHL PSHIRILGLK RVTGGFNSKN KCDARTYCYM
210 220 230 240 250
LPTFAFAHKD RDVQDESYRL SAETLQQVNR LLACYKGTHN FHNFTSQKGP
260 270 280 290 300
REPSARRYIL EMYCEEPFVR EGLEFAVIKV KGQSFMMHQI RKMVGLVVAI
310 320 330 340 350
VKGYAPESVL ERSWGEEKVD VPKAPGLGLV LERVHFEKYN QRFGGDGLHE
360 370 380 390 400
PLDWTQEEGK VTAFKEQYIY PTIVSTERDE RSMAQWLNTL PIHNFSGTAL
410 420
GAADTGAKVP SSLEGSEGDG DTD
Length:423
Mass (Da):47,502
Last modified:September 5, 2006 - v2
Checksum:iC71FDBF60F29295A
GO
Isoform 2 (identifier: Q9WU56-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:393
Mass (Da):44,093
Checksum:i4CE2BD836B15A54A
GO
Isoform 3 (identifier: Q9WU56-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     143-143: K → KPLRTSACFCLYTGFLFHW

Show »
Length:411
Mass (Da):46,238
Checksum:i152A6D10552F88FA
GO
Isoform 4 (identifier: Q9WU56-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     98-143: Missing.

Note: No experimental confirmation available.
Show »
Length:347
Mass (Da):38,927
Checksum:i326246638DFAD35F
GO

Sequence cautioni

The sequence BAA95047 differs from that shown. Reason: Frameshift at position 230.Curated
The sequence BAC40817 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48K → E in BAC40817 (PubMed:16141072).Curated1
Sequence conflicti52G → D in BAC40817 (PubMed:16141072).Curated1
Sequence conflicti406G → V in AAH21446 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0201141 – 30Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsAdd BLAST30
Alternative sequenceiVSP_02179198 – 143Missing in isoform 4. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_021792143K → KPLRTSACFCLYTGFLFHW in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116237 mRNA Translation: AAD21041.1
AF269250 Genomic DNA Translation: AAG35307.1
AF269250 Genomic DNA Translation: AAG35308.1
AB041563 mRNA Translation: BAA95047.1 Frameshift.
AK089258 mRNA Translation: BAC40817.1 Different initiation.
AK138570 mRNA Translation: BAE23701.1
BC021446 mRNA Translation: AAH21446.2
BC034359 mRNA Translation: AAH34359.2
CCDSiCCDS19531.1 [Q9WU56-1]
CCDS39212.1 [Q9WU56-2]
CCDS84929.1 [Q9WU56-4]
RefSeqiNP_001020733.1, NM_001025562.2 [Q9WU56-2]
NP_001334319.1, NM_001347390.1 [Q9WU56-4]
NP_062674.2, NM_019700.4 [Q9WU56-1]
XP_011247847.1, XM_011249545.2
XP_017176503.1, XM_017321014.1 [Q9WU56-2]
XP_017176504.1, XM_017321015.1 [Q9WU56-2]
UniGeneiMm.23528

Genome annotation databases

EnsembliENSMUST00000031481; ENSMUSP00000031481; ENSMUSG00000029507 [Q9WU56-2]
ENSMUST00000031483; ENSMUSP00000031483; ENSMUSG00000029507 [Q9WU56-1]
ENSMUST00000112426; ENSMUSP00000108045; ENSMUSG00000029507 [Q9WU56-4]
ENSMUST00000170468; ENSMUSP00000130814; ENSMUSG00000029507 [Q9WU56-2]
GeneIDi56361
KEGGimmu:56361
UCSCiuc008yrm.1 mouse [Q9WU56-4]
uc008yrn.1 mouse [Q9WU56-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRUA_MOUSE
AccessioniPrimary (citable) accession number: Q9WU56
Secondary accession number(s): Q3UUC6
, Q791J1, Q8C250, Q8VDQ3, Q9EQD1, Q9JJE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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