Reviewed, UniProtKB/Swiss-Prot Q9WU47 (B3GA3_CRIGR)

Names and origin

Protein names

Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
    EC=2.4.1.135
Alternative name(s):
    Beta-1,3-glucuronyltransferase 3
    Glucuronosyltransferase-I
      Short name=GlcAT-I
    UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
      Short name=GlcUAT-I

Gene names

Name:

B3GAT3

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Highest activity seen with Gal-beta-1,3-Gal-beta-O-R (where R=naphthalenemethanol or benzyl alcohol).
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Liver, brain and heart. Moderate expression seen in lung, skeletal muscle, kidney and testis.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3		PRO_0000195175
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 335	307	Lumenal Potential
Sites
	Active site	281	1	Proton acceptor By similarity
	Metal binding	196	1	Manganese By similarity
Amino acid modifications
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WU47-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B4FD1D1645BDCD1C	FASTA	335	37,095
	10 20 30 40 50 60 MKLKLKNVFL AYFLVSIAGL LYALVQLGQP CDCLPPLRAA AEQLRQKDLR ISQLQADLRR 70 80 90 100 110 120 PPPVPAQPPE PEALPTIYVI TPTYARLVQK AELVRLSQTL SLVPRLHWLL VEDAESPTPL 130 140 150 160 170 180 VSGLLAASGL LFTHLAVLTP KAQRLREGEP GWVRPRGVEQ RNKALDWLRG KGGAVGGEKD 190 200 210 220 230 240 PPPPGTQGVV YFADDDNTYS RELFKEMRWT RGVSVWPVGL VGGLRFEGPR VQDGRVVGFH 250 260 270 280 290 300 TAWEPNRPFP LDMAGFAVAL PLLLAKPNAQ FDATAPRGHL ESSLLSHLVD PKDLEPRAAN 310 320 330 CTQVLVWHTR TEKPKMKQEE QLQRQGQGSD PAIEV

References

[1]	"Formation of HNK-1 determinants and the glycosaminoglycan tetrasaccharide linkage region by UDP-GlcUA:Galactose beta1, 3-glucuronosyltransferases." Wei G., Bai X., Sarkar A.K., Esko J.D. J. Biol. Chem. 274:7857-7864(1999) [PubMed: 10075678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF113703 mRNA. Translation: AAD22007.1.
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
SMR	Q9WU47. Positions 76-335.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Phylogenomic databases
HOVERGEN	Q9WU47.
Enzyme and pathway databases
BRENDA	2.4.1.135. 824.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GA3_CRIGR

Accession

Primary (citable) accession number: Q9WU47

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1999
Last modified:	October 13, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)