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Protein

Inner nuclear membrane protein Man1

Gene

Lemd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi717 – 73620By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: MGI
  • blood vessel endothelial cell migration involved in intussusceptive angiogenesis Source: MGI
  • negative regulation of activin receptor signaling pathway Source: MGI
  • negative regulation of BMP signaling pathway Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • nucleus organization Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of extracellular matrix organization Source: MGI
  • skeletal muscle cell differentiation Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner nuclear membrane protein Man1
Alternative name(s):
LEM domain-containing protein 3
Gene namesi
Name:Lemd3
Synonyms:Man1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3580376. Lemd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei486 – 50621HelicalSequence analysisAdd
BLAST
Transmembranei637 – 65721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 921921Inner nuclear membrane protein Man1PRO_0000206150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281PhosphoserineBy similarity
Modified residuei136 – 1361PhosphoserineBy similarity
Modified residuei137 – 1371PhosphoserineBy similarity
Modified residuei140 – 1401PhosphoserineCombined sources
Modified residuei261 – 2611PhosphoserineBy similarity
Modified residuei263 – 2631PhosphoserineCombined sources
Modified residuei287 – 2871PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity
Modified residuei787 – 7871PhosphoserineBy similarity
Modified residuei893 – 8931PhosphothreonineBy similarity
Modified residuei921 – 9211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9WU40.
MaxQBiQ9WU40.
PaxDbiQ9WU40.
PRIDEiQ9WU40.

PTM databases

iPTMnetiQ9WU40.
PhosphoSiteiQ9WU40.

Expressioni

Gene expression databases

CleanExiMM_LEMD3.

Interactioni

Subunit structurei

Interacts with SMAD1, SMAD2, SMAD3 and SMAD5. Binds to both phosphorylated and unphosphorylated R-SMADS (By similarity).By similarity

Protein-protein interaction databases

BioGridi237587. 1 interaction.
IntActiQ9WU40. 1 interaction.
MINTiMINT-4101110.
STRINGi10090.ENSMUSP00000113103.

Structurei

3D structure databases

ProteinModelPortaliQ9WU40.
SMRiQ9WU40. Positions 10-51, 664-790, 793-898.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 5145LEMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni709 – 921213Interaction with SMAD1, SMAD2, SMAD3 and SMAD5By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 6211Poly-GlnAdd
BLAST
Compositional biasi74 – 796Poly-Asn
Compositional biasi151 – 1577Poly-Gly
Compositional biasi264 – 2729Poly-Glu
Compositional biasi329 – 3324Poly-Ala
Compositional biasi349 – 3579Poly-Gly
Compositional biasi424 – 4296Poly-Ala
Compositional biasi645 – 6506Poly-Leu
Compositional biasi669 – 6724Poly-Glu

Sequence similaritiesi

Contains 1 LEM domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0147. Eukaryota.
ENOG410XP20. LUCA.
HOGENOMiHOG000063699.
HOVERGENiHBG006319.
InParanoidiQ9WU40.
KOiK19410.

Family and domain databases

Gene3Di1.10.720.40. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR018996. Inner-Nucl-membr_MAN1.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF09402. MSC. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF63451. SSF63451. 1 hit.
PROSITEiPS50954. LEM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU40-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAATAAAAP QQLSDEELFS QLRRYGLSPG PVTESTRPVY LKKLKKLREE
60 70 80 90 100
EQQQQQQQQQ QQHRAGGRGN KTRNSNNNNT ATAMGGRPGS GDLAYLRSPA
110 120 130 140 150
GLGRLSASAA ESPVAGGSGG AAAVPAAGSK VLLGFSSDES DVEASPREQA
160 170 180 190 200
GGGGGGGARR DRAALQYRGL RAPPAPPAAG EVTGGHPGER RKPHSWWGAR
210 220 230 240 250
RPAGPEPQPP AAGSDGAAED ADEELADGED RDPEAEEPLW ASRAVNGSRL
260 270 280 290 300
LPYSSCREHY SDSEEEEEEG EEDGDVAPAR QVLKDDSLAR HRPRRSHSKP
310 320 330 340 350
FSALTAKSGG SRQETSVQGG GALAMNDRAA AAGSLDRSRN LEEAAAEPGG
360 370 380 390 400
GGGGGCGCDP VDSIPRYRAG AKKLAPLLSP PSPDGDSTLE SPTGPLLKTN
410 420 430 440 450
NHIGGGAFGV DSPGLYANSL PPGATAAAAP GTLRINHANH TGSNHTYLKT
460 470 480 490 500
AYGKPKLCEP EEELLQQFKR EEVSPTGSFS AHYLSMFLLT AACLFFLILG
510 520 530 540 550
LTYLGMRGTG VPEDGGLIKN PFDETFGKIQ ESEKNLLMST LYKLHDRLAQ
560 570 580 590 600
IAGDHECGSS SQRMLSVQEA AAYLKNLGPE YEDVFNTSLL WIFKNGKDVG
610 620 630 640 650
IRCVGYGPEE DLTNITDVQF LQSTRPQMPF WCRFRRAFIT VTHRLLLLCL
660 670 680 690 700
GVVLVCVALR YMRYRWTKEE EETRQMYDMV VKIIDVLRSH NEACQETKDL
710 720 730 740 750
QPYMPLPHVR DSLIQPQDRK KMKKVWDRAV DFLAANESRV RTETRRVGGA
760 770 780 790 800
DFLVWRWIQP SASCDKTLVI PSKVWQGQAF HLDRRNSPPN SLTPCLKIRN
810 820 830 840 850
MFDPVMEIGD HWHLAIQEAI LEKCSDNDGI VHIAVDRNSR EGCVYVKCLS
860 870 880 890 900
PEYAGKAFKA LHGSWFDGKL VTVKYLRLDR YHHRFPQALT CNTPLKPANK
910 920
HMNSLSHLRL RTGLANSQGS S
Length:921
Mass (Da):100,307
Last modified:July 10, 2007 - v2
Checksum:iEBFA12FE19D79F3D
GO
Isoform 2 (identifier: Q9WU40-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     575-575: K → KVHLVFKITAENLCFKTLSDASN

Note: No experimental confirmation available.
Show »
Length:943
Mass (Da):102,739
Checksum:iC1A46ECEB922CD4C
GO

Sequence cautioni

The sequence BC082610 differs from that shown. Reason: Frameshift at position 840. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti804 – 8041P → Q in BAE24418 (PubMed:16141072).Curated
Sequence conflicti861 – 8611L → F in BAE24418 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei575 – 5751K → KVHLVFKITAENLCFKTLSD ASN in isoform 2. 1 PublicationVSP_026782

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC140381 Genomic DNA. No translation available.
AF112300 mRNA. Translation: AAD31594.1.
BC082610 mRNA. No translation available.
AK140538 mRNA. Translation: BAE24418.1.
RefSeqiNP_001074662.2. NM_001081193.2.
UniGeneiMm.339371.

Genome annotation databases

GeneIDi380664.
KEGGimmu:380664.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC140381 Genomic DNA. No translation available.
AF112300 mRNA. Translation: AAD31594.1.
BC082610 mRNA. No translation available.
AK140538 mRNA. Translation: BAE24418.1.
RefSeqiNP_001074662.2. NM_001081193.2.
UniGeneiMm.339371.

3D structure databases

ProteinModelPortaliQ9WU40.
SMRiQ9WU40. Positions 10-51, 664-790, 793-898.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237587. 1 interaction.
IntActiQ9WU40. 1 interaction.
MINTiMINT-4101110.
STRINGi10090.ENSMUSP00000113103.

PTM databases

iPTMnetiQ9WU40.
PhosphoSiteiQ9WU40.

Proteomic databases

EPDiQ9WU40.
MaxQBiQ9WU40.
PaxDbiQ9WU40.
PRIDEiQ9WU40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi380664.
KEGGimmu:380664.

Organism-specific databases

CTDi23592.
MGIiMGI:3580376. Lemd3.

Phylogenomic databases

eggNOGiKOG0147. Eukaryota.
ENOG410XP20. LUCA.
HOGENOMiHOG000063699.
HOVERGENiHBG006319.
InParanoidiQ9WU40.
KOiK19410.

Miscellaneous databases

ChiTaRSiLemd3. mouse.
PROiQ9WU40.
SOURCEiSearch...

Gene expression databases

CleanExiMM_LEMD3.

Family and domain databases

Gene3Di1.10.720.40. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR018996. Inner-Nucl-membr_MAN1.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF09402. MSC. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF63451. SSF63451. 1 hit.
PROSITEiPS50954. LEM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAN1_MOUSE
AccessioniPrimary (citable) accession number: Q9WU40
Secondary accession number(s): Q0VGU6, Q3USB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 10, 2007
Last modified: July 6, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.