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Q9WU39 (SCNNG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amiloride-sensitive sodium channel subunit gamma
Alternative name(s):
Epithelial Na(+) channel subunit gamma
Short name=Gamma-ENaC
Gamma-NaCH
Nonvoltage-gated sodium channel 1 subunit gamma
SCNEG
Gene names
Name:Scnn1g
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

Enzyme regulation

Activated by WNK1, WNK2, WNK3 and WNK4. Ref.6

Subunit structure

Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2A. Interacts with the full length immature form of PCSK9 (pro-PCSK9) By similarity. Ref.3 Ref.4 Ref.5

Subcellular location

Apical cell membrane; Multi-pass membrane protein By similarity. Note: Apical membrane of epithelial cells By similarity.

Tissue specificity

Lung and kidney.

Post-translational modification

Phosphorylated on serine and threonine residues By similarity.

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation By similarity.

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation By similarity.

Sequence similarities

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Sensory transduction
Sodium transport
Taste
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   Molecular functionIon channel
Sodium channel
   PTMGlycoprotein
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmulticellular organismal water homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

response to stimulus

Inferred from electronic annotation. Source: UniProtKB-KW

sensory perception of taste

Inferred from electronic annotation. Source: UniProtKB-KW

sodium ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

sodium ion transmembrane transport

Inferred from direct assay Ref.1PubMed 16941024. Source: GOC

sodium ion transport

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

external side of plasma membrane

Inferred from direct assay PubMed 17715136. Source: MGI

integral component of membrane

Inferred from direct assay Ref.1. Source: MGI

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 17715136PubMed 21413931. Source: MGI

sodium channel complex

Inferred from genetic interaction PubMed 17715136. Source: MGI

   Molecular_functionWW domain binding

Inferred from physical interaction PubMed 10212229. Source: MGI

ligand-gated sodium channel activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Amiloride-sensitive sodium channel subunit gamma
PRO_0000181277

Regions

Topological domain1 – 5555Cytoplasmic By similarity
Transmembrane56 – 7924Helical; By similarity
Topological domain80 – 529450Extracellular By similarity
Transmembrane530 – 56132Helical; By similarity
Topological domain562 – 65594Cytoplasmic By similarity

Amino acid modifications

Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation5031N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9WU39 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: ABADDA9D6160596D

FASTA65574,635
        10         20         30         40         50         60 
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCLNTNTHG CRRIVVSRGR LRRLLWIAFT 

        70         80         90        100        110        120 
LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC NINPYKYSAV SDLLTDLDSE 

       130        140        150        160        170        180 
TKQALLSLYG VKDVLDSTPR KRREAGSMRS TWEGTPPRFL NLIPLLVFNE NEKGKARDFF 

       190        200        210        220        230        240 
TGRKRKISGK IIHKASNVMH VHESKKLVGF QLCSNDTSDC ATYTFSSGIN AIQEWYKLHY 

       250        260        270        280        290        300 
MNIMAQVPLE KKINMSYSAE ELLVTCFFDG MSCDARNFTL FHHPMYGNCY TFNNRENATI 

       310        320        330        340        350        360 
LSTSMGGSEY GLQVILYINE DEYNPFLVSS TGAKVLVHQQ NEYPFIEDVG TEIETAMSTS 

       370        380        390        400        410        420 
IGMHLTESFK LSEPYSQCTE DGSDVPVTNI YNAAYSLQIC LYSCFQTKMV EKCGCAQYSQ 

       430        440        450        460        470        480 
PLPPAANYCN YQQHPNWMYC YYQLYQAFVR EELGCQSVCK QSCSFKEWTL TTSLAQWPSE 

       490        500        510        520        530        540 
ASEKWLLNVL TWDQSQQINK KLNKTDLAKL LIFYKDLNQR SIMESPANSI EMLLSNFGGQ 

       550        560        570        580        590        600 
LGLWMSCSVV CVIEIIEVFF IDFFSIIARR QWQKAKDWWA RRRTPPSTET PSSQQGQDNP 

       610        620        630        640        650 
ALDTDDDLPT FTSAMRLPPA PEAPVPGTPP PRYNTLRLDS AFSSQLTDTQ LTNEF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and functional expression of the mouse epithelial sodium channel."
Ahn Y.J., Brooker D.R., Kosari F., Harte B.J., Li J., Mackler S.A., Kleyman T.R.
Am. J. Physiol. 277:F121-F129(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[3]"The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel."
Harvey K.F., Dinudom A., Cook D.I., Kumar S.
J. Biol. Chem. 276:8597-8601(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
[4]"The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels."
Fotia A.B., Dinudom A., Shearwin K.E., Koch J.-P., Korbmacher C., Cook D.I., Kumar S.
FASEB J. 17:70-72(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEDD4L.
[5]"Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2."
Fotia A.B., Ekberg J., Adams D.J., Cook D.I., Poronnik P., Kumar S.
J. Biol. Chem. 279:28930-28935(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
[6]"Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members."
Heise C.J., Xu B.E., Deaton S.L., Cha S.K., Cheng C.J., Earnest S., Sengupta S., Juang Y.C., Stippec S., Xu Y., Zhao Y., Huang C.L., Cobb M.H.
J. Biol. Chem. 285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF112187 mRNA. Translation: AAD21246.1.
BC021338 mRNA. Translation: AAH21338.1.
RefSeqNP_035456.1. NM_011326.2.
UniGeneMm.35247.

3D structure databases

ProteinModelPortalQ9WU39.
SMRQ9WU39. Positions 50-563.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203107. 4 interactions.
MINTMINT-90115.

PTM databases

PhosphoSiteQ9WU39.

Proteomic databases

PRIDEQ9WU39.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000221; ENSMUSP00000000221; ENSMUSG00000000216.
GeneID20278.
KEGGmmu:20278.
UCSCuc009jnv.1. mouse.

Organism-specific databases

CTD6340.
MGIMGI:104695. Scnn1g.

Phylogenomic databases

eggNOGNOG294559.
GeneTreeENSGT00640000091377.
HOGENOMHOG000236286.
HOVERGENHBG058435.
InParanoidQ9WU39.
KOK04827.
OMANWMYCYY.
OrthoDBEOG7T1R9N.
PhylomeDBQ9WU39.
TreeFamTF330663.

Gene expression databases

BgeeQ9WU39.
CleanExMM_SCNN1G.
GenevestigatorQ9WU39.

Family and domain databases

InterProIPR004724. EnaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERPTHR11690. PTHR11690. 1 hit.
PfamPF00858. ASC. 1 hit.
[Graphical view]
PRINTSPR01078. AMINACHANNEL.
TIGRFAMsTIGR00859. ENaC. 1 hit.
PROSITEPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio297971.
PMAP-CutDBQ9WU39.
PROQ9WU39.
SOURCESearch...

Entry information

Entry nameSCNNG_MOUSE
AccessionPrimary (citable) accession number: Q9WU39
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot