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Protein

Amiloride-sensitive sodium channel subunit gamma

Gene

Scnn1g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity1 Publication

Enzyme regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.1 Publication

GO - Molecular functioni

  1. ligand-gated sodium channel activity Source: MGI
  2. WW domain binding Source: MGI

GO - Biological processi

  1. multicellular organismal water homeostasis Source: UniProtKB
  2. response to stimulus Source: UniProtKB-KW
  3. sensory perception of taste Source: UniProtKB-KW
  4. sodium ion homeostasis Source: UniProtKB
  5. sodium ion transmembrane transport Source: MGI
  6. sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sensory transduction, Sodium transport, Taste, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_346473. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit gamma
Alternative name(s):
Epithelial Na(+) channel subunit gamma
Short name:
Gamma-ENaC
Gamma-NaCH
Nonvoltage-gated sodium channel 1 subunit gamma
SCNEG
Gene namesi
Name:Scnn1g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104695. Scnn1g.

Subcellular locationi

  1. Apical cell membrane 1 Publication; Multi-pass membrane protein By similarity

  2. Note: Apical membrane of epithelial cells.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555CytoplasmicBy similarityAdd
BLAST
Transmembranei56 – 7621Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini77 – 547471ExtracellularBy similarityAdd
BLAST
Transmembranei548 – 56821Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini569 – 65587CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. external side of plasma membrane Source: MGI
  3. extracellular vesicular exosome Source: MGI
  4. integral component of membrane Source: MGI
  5. integral component of plasma membrane Source: UniProtKB
  6. membrane Source: MGI
  7. sodium channel complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Amiloride-sensitive sodium channel subunit gammaPRO_0000181277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.By similarity
Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.By similarity
Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9WU39.

PTM databases

PhosphoSiteiQ9WU39.

Miscellaneous databases

PMAP-CutDBQ9WU39.

Expressioni

Tissue specificityi

Lung and kidney.1 Publication

Gene expression databases

BgeeiQ9WU39.
CleanExiMM_SCNN1G.
GenevestigatoriQ9WU39.

Interactioni

Subunit structurei

Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit also exists in some organisms and can replace the alpha subunit to form alternative channels (Probable). Interacts with NEDD4; via the WW domains (PubMed:11244092, PubMed:15123669). Interacts with NEDD4L; via the WW domains (PubMed:12424229, PubMed:11244092, PubMed:15123669). Interacts with WWP1; via the WW domains (By similarity). Interacts with WWP2; via the WW domains. Interacts with the full length immature form of PCSK9 (pro-PCSK9) (By similarity).By similarity1 Publication3 Publications

Protein-protein interaction databases

BioGridi203107. 4 interactions.
MINTiMINT-90115.

Structurei

3D structure databases

ProteinModelPortaliQ9WU39.
SMRiQ9WU39. Positions 261-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294559.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000236286.
HOVERGENiHBG058435.
InParanoidiQ9WU39.
KOiK04827.
OMAiNWMYCYY.
OrthoDBiEOG7T1R9N.
PhylomeDBiQ9WU39.
TreeFamiTF330663.

Family and domain databases

InterProiIPR004724. EnaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WU39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCLNTNTHG CRRIVVSRGR
60 70 80 90 100
LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC
110 120 130 140 150
NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKDVLDSTPR KRREAGSMRS
160 170 180 190 200
TWEGTPPRFL NLIPLLVFNE NEKGKARDFF TGRKRKISGK IIHKASNVMH
210 220 230 240 250
VHESKKLVGF QLCSNDTSDC ATYTFSSGIN AIQEWYKLHY MNIMAQVPLE
260 270 280 290 300
KKINMSYSAE ELLVTCFFDG MSCDARNFTL FHHPMYGNCY TFNNRENATI
310 320 330 340 350
LSTSMGGSEY GLQVILYINE DEYNPFLVSS TGAKVLVHQQ NEYPFIEDVG
360 370 380 390 400
TEIETAMSTS IGMHLTESFK LSEPYSQCTE DGSDVPVTNI YNAAYSLQIC
410 420 430 440 450
LYSCFQTKMV EKCGCAQYSQ PLPPAANYCN YQQHPNWMYC YYQLYQAFVR
460 470 480 490 500
EELGCQSVCK QSCSFKEWTL TTSLAQWPSE ASEKWLLNVL TWDQSQQINK
510 520 530 540 550
KLNKTDLAKL LIFYKDLNQR SIMESPANSI EMLLSNFGGQ LGLWMSCSVV
560 570 580 590 600
CVIEIIEVFF IDFFSIIARR QWQKAKDWWA RRRTPPSTET PSSQQGQDNP
610 620 630 640 650
ALDTDDDLPT FTSAMRLPPA PEAPVPGTPP PRYNTLRLDS AFSSQLTDTQ

LTNEF
Length:655
Mass (Da):74,635
Last modified:November 1, 1999 - v1
Checksum:iABADDA9D6160596D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112187 mRNA. Translation: AAD21246.1.
BC021338 mRNA. Translation: AAH21338.1.
CCDSiCCDS21803.1.
RefSeqiNP_035456.1. NM_011326.2.
UniGeneiMm.35247.

Genome annotation databases

EnsembliENSMUST00000000221; ENSMUSP00000000221; ENSMUSG00000000216.
GeneIDi20278.
KEGGimmu:20278.
UCSCiuc009jnv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112187 mRNA. Translation: AAD21246.1.
BC021338 mRNA. Translation: AAH21338.1.
CCDSiCCDS21803.1.
RefSeqiNP_035456.1. NM_011326.2.
UniGeneiMm.35247.

3D structure databases

ProteinModelPortaliQ9WU39.
SMRiQ9WU39. Positions 261-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203107. 4 interactions.
MINTiMINT-90115.

PTM databases

PhosphoSiteiQ9WU39.

Proteomic databases

PRIDEiQ9WU39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000221; ENSMUSP00000000221; ENSMUSG00000000216.
GeneIDi20278.
KEGGimmu:20278.
UCSCiuc009jnv.1. mouse.

Organism-specific databases

CTDi6340.
MGIiMGI:104695. Scnn1g.

Phylogenomic databases

eggNOGiNOG294559.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000236286.
HOVERGENiHBG058435.
InParanoidiQ9WU39.
KOiK04827.
OMAiNWMYCYY.
OrthoDBiEOG7T1R9N.
PhylomeDBiQ9WU39.
TreeFamiTF330663.

Enzyme and pathway databases

ReactomeiREACT_346473. Stimuli-sensing channels.

Miscellaneous databases

NextBioi297971.
PMAP-CutDBQ9WU39.
PROiQ9WU39.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WU39.
CleanExiMM_SCNN1G.
GenevestigatoriQ9WU39.

Family and domain databases

InterProiIPR004724. EnaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional expression of the mouse epithelial sodium channel."
    Ahn Y.J., Brooker D.R., Kosari F., Harte B.J., Li J., Mackler S.A., Kleyman T.R.
    Am. J. Physiol. 277:F121-F129(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel."
    Harvey K.F., Dinudom A., Cook D.I., Kumar S.
    J. Biol. Chem. 276:8597-8601(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
  4. "The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels."
    Fotia A.B., Dinudom A., Shearwin K.E., Koch J.-P., Korbmacher C., Cook D.I., Kumar S.
    FASEB J. 17:70-72(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NEDD4L.
  5. "Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2."
    Fotia A.B., Ekberg J., Adams D.J., Cook D.I., Poronnik P., Kumar S.
    J. Biol. Chem. 279:28930-28935(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
  6. "Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members."
    Heise C.J., Xu B.E., Deaton S.L., Cha S.K., Cheng C.J., Earnest S., Sengupta S., Juang Y.C., Stippec S., Xu Y., Zhao Y., Huang C.L., Cobb M.H.
    J. Biol. Chem. 285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiSCNNG_MOUSE
AccessioniPrimary (citable) accession number: Q9WU39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.