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Q9WU39

- SCNNG_MOUSE

UniProt

Q9WU39 - SCNNG_MOUSE

Protein

Amiloride-sensitive sodium channel subunit gamma

Gene

Scnn1g

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

    Enzyme regulationi

    Activated by WNK1, WNK2, WNK3 and WNK4.1 Publication

    GO - Molecular functioni

    1. ligand-gated sodium channel activity Source: MGI
    2. WW domain binding Source: MGI

    GO - Biological processi

    1. multicellular organismal water homeostasis Source: UniProtKB
    2. response to stimulus Source: UniProtKB-KW
    3. sensory perception of taste Source: UniProtKB-KW
    4. sodium ion homeostasis Source: UniProtKB
    5. sodium ion transmembrane transport Source: GOC
    6. sodium ion transport Source: MGI

    Keywords - Molecular functioni

    Ion channel, Sodium channel

    Keywords - Biological processi

    Ion transport, Sensory transduction, Sodium transport, Taste, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amiloride-sensitive sodium channel subunit gamma
    Alternative name(s):
    Epithelial Na(+) channel subunit gamma
    Short name:
    Gamma-ENaC
    Gamma-NaCH
    Nonvoltage-gated sodium channel 1 subunit gamma
    SCNEG
    Gene namesi
    Name:Scnn1g
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:104695. Scnn1g.

    Subcellular locationi

    Apical cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Apical membrane of epithelial cells.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. external side of plasma membrane Source: MGI
    3. integral component of membrane Source: MGI
    4. integral component of plasma membrane Source: UniProtKB
    5. membrane Source: MGI
    6. sodium channel complex Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 655655Amiloride-sensitive sodium channel subunit gammaPRO_0000181277Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated on serine and threonine residues.By similarity
    Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.By similarity
    ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ9WU39.

    PTM databases

    PhosphoSiteiQ9WU39.

    Miscellaneous databases

    PMAP-CutDBQ9WU39.

    Expressioni

    Tissue specificityi

    Lung and kidney.

    Gene expression databases

    BgeeiQ9WU39.
    CleanExiMM_SCNN1G.
    GenevestigatoriQ9WU39.

    Interactioni

    Subunit structurei

    Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2A. Interacts with the full length immature form of PCSK9 (pro-PCSK9) By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203107. 4 interactions.
    MINTiMINT-90115.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WU39.
    SMRiQ9WU39. Positions 261-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555CytoplasmicBy similarityAdd
    BLAST
    Topological domaini80 – 529450ExtracellularBy similarityAdd
    BLAST
    Topological domaini562 – 65594CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 7924HelicalBy similarityAdd
    BLAST
    Transmembranei530 – 56132HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG294559.
    GeneTreeiENSGT00640000091377.
    HOGENOMiHOG000236286.
    HOVERGENiHBG058435.
    InParanoidiQ9WU39.
    KOiK04827.
    OMAiNWMYCYY.
    OrthoDBiEOG7T1R9N.
    PhylomeDBiQ9WU39.
    TreeFamiTF330663.

    Family and domain databases

    InterProiIPR004724. EnaC.
    IPR001873. Na+channel_ASC.
    IPR020903. Na+channel_ASC_CS.
    [Graphical view]
    PANTHERiPTHR11690. PTHR11690. 1 hit.
    PfamiPF00858. ASC. 1 hit.
    [Graphical view]
    PRINTSiPR01078. AMINACHANNEL.
    TIGRFAMsiTIGR00859. ENaC. 1 hit.
    PROSITEiPS01206. ASC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WU39-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPGEKIKAK IKKNLPVRGP QAPTIKDLMH WYCLNTNTHG CRRIVVSRGR    50
    LRRLLWIAFT LTAVALIIWQ CALLVFSFYT VSVSIKVHFQ KLDFPAVTIC 100
    NINPYKYSAV SDLLTDLDSE TKQALLSLYG VKDVLDSTPR KRREAGSMRS 150
    TWEGTPPRFL NLIPLLVFNE NEKGKARDFF TGRKRKISGK IIHKASNVMH 200
    VHESKKLVGF QLCSNDTSDC ATYTFSSGIN AIQEWYKLHY MNIMAQVPLE 250
    KKINMSYSAE ELLVTCFFDG MSCDARNFTL FHHPMYGNCY TFNNRENATI 300
    LSTSMGGSEY GLQVILYINE DEYNPFLVSS TGAKVLVHQQ NEYPFIEDVG 350
    TEIETAMSTS IGMHLTESFK LSEPYSQCTE DGSDVPVTNI YNAAYSLQIC 400
    LYSCFQTKMV EKCGCAQYSQ PLPPAANYCN YQQHPNWMYC YYQLYQAFVR 450
    EELGCQSVCK QSCSFKEWTL TTSLAQWPSE ASEKWLLNVL TWDQSQQINK 500
    KLNKTDLAKL LIFYKDLNQR SIMESPANSI EMLLSNFGGQ LGLWMSCSVV 550
    CVIEIIEVFF IDFFSIIARR QWQKAKDWWA RRRTPPSTET PSSQQGQDNP 600
    ALDTDDDLPT FTSAMRLPPA PEAPVPGTPP PRYNTLRLDS AFSSQLTDTQ 650
    LTNEF 655
    Length:655
    Mass (Da):74,635
    Last modified:November 1, 1999 - v1
    Checksum:iABADDA9D6160596D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF112187 mRNA. Translation: AAD21246.1.
    BC021338 mRNA. Translation: AAH21338.1.
    CCDSiCCDS21803.1.
    RefSeqiNP_035456.1. NM_011326.2.
    UniGeneiMm.35247.

    Genome annotation databases

    EnsembliENSMUST00000000221; ENSMUSP00000000221; ENSMUSG00000000216.
    GeneIDi20278.
    KEGGimmu:20278.
    UCSCiuc009jnv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF112187 mRNA. Translation: AAD21246.1 .
    BC021338 mRNA. Translation: AAH21338.1 .
    CCDSi CCDS21803.1.
    RefSeqi NP_035456.1. NM_011326.2.
    UniGenei Mm.35247.

    3D structure databases

    ProteinModelPortali Q9WU39.
    SMRi Q9WU39. Positions 261-555.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203107. 4 interactions.
    MINTi MINT-90115.

    PTM databases

    PhosphoSitei Q9WU39.

    Proteomic databases

    PRIDEi Q9WU39.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000221 ; ENSMUSP00000000221 ; ENSMUSG00000000216 .
    GeneIDi 20278.
    KEGGi mmu:20278.
    UCSCi uc009jnv.1. mouse.

    Organism-specific databases

    CTDi 6340.
    MGIi MGI:104695. Scnn1g.

    Phylogenomic databases

    eggNOGi NOG294559.
    GeneTreei ENSGT00640000091377.
    HOGENOMi HOG000236286.
    HOVERGENi HBG058435.
    InParanoidi Q9WU39.
    KOi K04827.
    OMAi NWMYCYY.
    OrthoDBi EOG7T1R9N.
    PhylomeDBi Q9WU39.
    TreeFami TF330663.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 297971.
    PMAP-CutDB Q9WU39.
    PROi Q9WU39.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WU39.
    CleanExi MM_SCNN1G.
    Genevestigatori Q9WU39.

    Family and domain databases

    InterProi IPR004724. EnaC.
    IPR001873. Na+channel_ASC.
    IPR020903. Na+channel_ASC_CS.
    [Graphical view ]
    PANTHERi PTHR11690. PTHR11690. 1 hit.
    Pfami PF00858. ASC. 1 hit.
    [Graphical view ]
    PRINTSi PR01078. AMINACHANNEL.
    TIGRFAMsi TIGR00859. ENaC. 1 hit.
    PROSITEi PS01206. ASC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional expression of the mouse epithelial sodium channel."
      Ahn Y.J., Brooker D.R., Kosari F., Harte B.J., Li J., Mackler S.A., Kleyman T.R.
      Am. J. Physiol. 277:F121-F129(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    3. "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel."
      Harvey K.F., Dinudom A., Cook D.I., Kumar S.
      J. Biol. Chem. 276:8597-8601(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
    4. "The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels."
      Fotia A.B., Dinudom A., Shearwin K.E., Koch J.-P., Korbmacher C., Cook D.I., Kumar S.
      FASEB J. 17:70-72(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEDD4L.
    5. "Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2."
      Fotia A.B., Ekberg J., Adams D.J., Cook D.I., Poronnik P., Kumar S.
      J. Biol. Chem. 279:28930-28935(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEDD4 AND NEDD4L.
    6. "Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members."
      Heise C.J., Xu B.E., Deaton S.L., Cha S.K., Cheng C.J., Earnest S., Sengupta S., Juang Y.C., Stippec S., Xu Y., Zhao Y., Huang C.L., Cobb M.H.
      J. Biol. Chem. 285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiSCNNG_MOUSE
    AccessioniPrimary (citable) accession number: Q9WU39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3