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Protein

Neuronal-specific septin-3

Gene

Sept3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).By similarityCurated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021GTPBy similarity
Binding sitei265 – 2651GTP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei280 – 2801GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758GTPBy similarity
Nucleotide bindingi208 – 2169GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal-specific septin-3
Alternative name(s):
G-septin
P40
Gene namesi
Name:Sept3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621745. Sept3.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: RGD
  • neuron projection Source: RGD
  • presynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi91 – 911S → A: Abolishes phosphorylation by PKG. 1 Publication
Mutagenesisi92 – 921S → A: Increases phosphorylation by PKG. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Neuronal-specific septin-3PRO_0000287229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei91 – 911Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WU34.
PRIDEiQ9WU34.

PTM databases

iPTMnetiQ9WU34.
PhosphoSiteiQ9WU34.

Expressioni

Tissue specificityi

Brain-specific, with highest expression in the hippocampal CA3 region (at protein level).2 Publications

Developmental stagei

First expressed in the embryonic brain from E18. Levels increase during brain development until P7 and remain constant until P35. Further increase in adult brain (at protein level).1 Publication

Interactioni

Subunit structurei

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity).By similarity

Protein-protein interaction databases

BioGridi248552. 1 interaction.
IntActiQ9WU34. 2 interactions.
STRINGi10116.ENSRNOP00000010491.

Structurei

3D structure databases

ProteinModelPortaliQ9WU34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 331274Septin-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1547. Eukaryota.
COG5019. LUCA.
HOGENOMiHOG000233586.
HOVERGENiHBG065093.
InParanoidiQ9WU34.
KOiK16938.
PhylomeDBiQ9WU34.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
IPR008114. Septin3.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
PRINTSiPR01741. SEPTIN3.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU34-1) [UniParc]FASTAAdd to basket

Also known as: G-septin-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKGLPEART DTAMSELVPE PRPKPAVPMK PVSINSNLLG CIGIDTIIEQ
60 70 80 90 100
MRKKTMKTGF DFNIMVVGQS GLGKSTLVNT LFKSQVSRKA SSWNREEKIP
110 120 130 140 150
KTVEIKAIGH VIEEGGVKMK LTVIDTPGFG DQINNENCWE PIEKYINEQY
160 170 180 190 200
EKFLKEEVNI ARKKRIPDTR VHCCLYFISP TGHSLRPLDL EFMKHLSKVV
210 220 230 240 250
NVIPVIAKAD TMTLEEKSEF KQRVRKELEV NGIEFYPQKE FDEDLEDKTE
260 270 280 290 300
NDKIRQESMP FAVVGSDKEY QVNGKRVLGR KTPWGIIEVE NLNHCEFALL
310 320 330 340 350
RDFVIRTHLQ DLKEVTHNIH YETYRAKRLN DNGGLPPGEG LLGTVLPPVP

ATPCPTAE
Length:358
Mass (Da):40,598
Last modified:May 15, 2007 - v2
Checksum:i96F05B2CFD195599
GO
Isoform 2 (identifier: Q9WU34-2) [UniParc]FASTAAdd to basket

Also known as: G-septin-beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: MSKGLPEART...TGFDFNIMVV → MESNCHFPRP...PFLCLPFCAA
     338-358: GEGLLGTVLPPVPATPCPTAE → VSVDTEESH

Note: No experimental confirmation available.
Show »
Length:347
Mass (Da):39,622
Checksum:i93DCAF73722803A2
GO
Isoform 3 (identifier: Q9WU34-3) [UniParc]FASTAAdd to basket

Also known as: G-septin-gamma

The sequence of this isoform differs from the canonical sequence as follows:
     299-301: LLR → PRA
     302-358: Missing.

Note: No experimental confirmation available.
Show »
Length:301
Mass (Da):34,295
Checksum:iFD5EC186E7F8902F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411C → Y in AAD21037 (PubMed:10744683).Curated
Sequence conflicti117 – 1171V → I in AAD21035 (PubMed:10744683).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767MSKGL…NIMVV → MESNCHFPRPLSYLPKRHQF LPDAHSSSSNKAWPGPQLWS SISSQEGSAAQEADVGLSPF LCLPFCAA in isoform 2. 1 PublicationVSP_025401Add
BLAST
Alternative sequencei299 – 3013LLR → PRA in isoform 3. 1 PublicationVSP_025402
Alternative sequencei302 – 35857Missing in isoform 3. 1 PublicationVSP_025403Add
BLAST
Alternative sequencei338 – 35821GEGLL…CPTAE → VSVDTEESH in isoform 2. 1 PublicationVSP_025404Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111179 mRNA. Translation: AAD21035.1.
AF111180 mRNA. Translation: AAD21036.1.
AF111181 mRNA. Translation: AAD21037.1.
RefSeqiNP_062248.1. NM_019375.1.
UniGeneiRn.48803.

Genome annotation databases

GeneIDi56003.
KEGGirno:56003.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111179 mRNA. Translation: AAD21035.1.
AF111180 mRNA. Translation: AAD21036.1.
AF111181 mRNA. Translation: AAD21037.1.
RefSeqiNP_062248.1. NM_019375.1.
UniGeneiRn.48803.

3D structure databases

ProteinModelPortaliQ9WU34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248552. 1 interaction.
IntActiQ9WU34. 2 interactions.
STRINGi10116.ENSRNOP00000010491.

PTM databases

iPTMnetiQ9WU34.
PhosphoSiteiQ9WU34.

Proteomic databases

PaxDbiQ9WU34.
PRIDEiQ9WU34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56003.
KEGGirno:56003.

Organism-specific databases

CTDi55964.
RGDi621745. Sept3.

Phylogenomic databases

eggNOGiKOG1547. Eukaryota.
COG5019. LUCA.
HOGENOMiHOG000233586.
HOVERGENiHBG065093.
InParanoidiQ9WU34.
KOiK16938.
PhylomeDBiQ9WU34.

Miscellaneous databases

NextBioi611071.
PROiQ9WU34.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
IPR008114. Septin3.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
PRINTSiPR01741. SEPTIN3.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phosphorylation of a new brain-specific septin, G-septin, by cGMP-dependent protein kinase."
    Xue J., Wang X., Malladi C.S., Kinoshita M., Milburn P.J., Lengyel I., Rostas J.A., Robinson P.J.
    J. Biol. Chem. 275:10047-10056(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), PROTEIN SEQUENCE OF 60-74 AND 314-325, PHOSPHORYLATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-74; 227-239 AND 314-325, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  3. "Phosphorylation of septin 3 on Ser-91 by cGMP-dependent protein kinase-I in nerve terminals."
    Xue J., Milburn P.J., Hanna B.T., Graham M.E., Rostas J.A., Robinson P.J.
    Biochem. J. 381:753-760(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-91, MUTAGENESIS OF SER-91 AND SER-92.
  4. "Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals."
    Xue J., Tsang C.W., Gai W.-P., Malladi C.S., Trimble W.S., Rostas J.A.P., Robinson P.J.
    J. Neurochem. 91:579-590(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSEPT3_RAT
AccessioniPrimary (citable) accession number: Q9WU34
Secondary accession number(s): Q9R245, Q9WU35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: January 20, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.