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Protein

Tyrosine-protein phosphatase non-receptor type 4

Gene

Ptpn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act at junctions between the membrane and the cytoskeleton.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei820 – 8201SubstrateBy similarity
Active sitei852 – 8521Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei896 – 8961SubstrateBy similarity

GO - Molecular functioni

  • non-membrane spanning protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  • peptidyl-tyrosine dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 4 (EC:3.1.3.48)
Alternative name(s):
Testis-enriched protein tyrosine phosphatase
Gene namesi
Name:Ptpn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1099792. Ptpn4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Tyrosine-protein phosphatase non-receptor type 4PRO_0000320075Add
BLAST

Proteomic databases

PaxDbiQ9WU22.
PRIDEiQ9WU22.

PTM databases

PhosphoSiteiQ9WU22.

Expressioni

Tissue specificityi

Highly expressed in testis. Specifically expressed in spermatocytes and spermatids within seminiferous tubules (at protein level).1 Publication

Gene expression databases

CleanExiMM_PTPN4.
ExpressionAtlasiQ9WU22. baseline and differential.
GenevisibleiQ9WU22. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd247P241613EBI-7249866,EBI-7803400

Protein-protein interaction databases

IntActiQ9WU22. 1 interaction.
MINTiMINT-126937.
STRINGi10090.ENSMUSP00000067614.

Structurei

3D structure databases

ProteinModelPortaliQ9WU22.
SMRiQ9WU22. Positions 31-310, 513-913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 312284FERMPROSITE-ProRule annotationAdd
BLAST
Domaini517 – 58973PDZPROSITE-ProRule annotationAdd
BLAST
Domaini655 – 911257Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni852 – 8587Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007048.
HOVERGENiHBG008322.
InParanoidiQ9WU22.
KOiK18037.
OMAiHTCTDFW.
OrthoDBiEOG76HQ1B.
TreeFamiTF315900.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000927. Tyr-Ptase_nr3. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WU22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFRVNKHDQ
60 70 80 90 100
GQVLLDIVFK HLDLTERDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP
110 120 130 140 150
YNLNFRVKFF VSDPNKLQEE YTRYQYFLQI KQDILTGRLS CPCNTAALLA
160 170 180 190 200
SFAVQSELGD YNQSENLAGY LSDYSFIPNQ PQDFEKEIAK LHQQHVGLSP
210 220 230 240 250
AEAEFNYLNA ARTLELYGVE FHYARDQSNN EILIGVMSGG ILIYKNRVRM
260 270 280 290 300
NTFLWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKTLWKA
310 320 330 340 350
CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN
360 370 380 390 400
KDRVFARSPS KPLARKLMDW EVVSRNSLSD DRLETQSLPS RSPPGTPNHR
410 420 430 440 450
NSSFTQEATR VRPSSVGHLV DHVVHMSPSE DFVSQRSPSS TQANSIVLES
460 470 480 490 500
SPSQETPEDG QPPALPPKQS KKNSWNQIHF SNSQQDLVTH TNESFDVPSS
510 520 530 540 550
PEKSTPNGGI PHDNLVLIKM KPDENGRFGF NVKGGYDQKM PVIVSRVAPG
560 570 580 590 600
TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC EKHSGELVLL
610 620 630 640 650
VRPNAVYDVV EEKLESEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL
660 670 680 690 700
ITGTVLAQFD QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVLLKG
710 720 730 740 750
NEDYINANYI NMEIPSSSII NQYIACQGPL PHTCKDFWQM TWEQGSSMVV
760 770 780 790 800
MLTTQVERGR VKCHQYWPEP SESSSYGCYQ VTCHSEEGNP AYIFRKMTLF
810 820 830 840 850
NQEKNESRQL TQIQYTAWPD HGVPDDSSDF LDFVCHVRDQ RAGKEEPIIV
860 870 880 890 900
HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ
910 920
YRFVCEAILK VYEEGFVKPL TTSSNK
Length:926
Mass (Da):105,832
Last modified:September 18, 2013 - v2
Checksum:i73E465D804C9C5FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti311 – 3111D → G in AAD22773 (PubMed:11054567).Curated
Sequence conflicti359 – 3591P → S in AAD22773 (PubMed:11054567).Curated
Sequence conflicti741 – 7411T → I in AAD22773 (PubMed:11054567).Curated
Sequence conflicti781 – 7811V → A in AAD22773 (PubMed:11054567).Curated
Sequence conflicti800 – 8001F → I in AAD22773 (PubMed:11054567).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106702 mRNA. Translation: AAD22773.1.
AC123955 Genomic DNA. No translation available.
AC124760 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39812.1.
CCDSiCCDS15227.1.
RefSeqiNP_064317.2. NM_019933.2.
UniGeneiMm.458796.

Genome annotation databases

EnsembliENSMUST00000064091; ENSMUSP00000067614; ENSMUSG00000026384.
GeneIDi19258.
KEGGimmu:19258.
UCSCiuc007ciz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106702 mRNA. Translation: AAD22773.1.
AC123955 Genomic DNA. No translation available.
AC124760 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39812.1.
CCDSiCCDS15227.1.
RefSeqiNP_064317.2. NM_019933.2.
UniGeneiMm.458796.

3D structure databases

ProteinModelPortaliQ9WU22.
SMRiQ9WU22. Positions 31-310, 513-913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WU22. 1 interaction.
MINTiMINT-126937.
STRINGi10090.ENSMUSP00000067614.

PTM databases

PhosphoSiteiQ9WU22.

Proteomic databases

PaxDbiQ9WU22.
PRIDEiQ9WU22.

Protocols and materials databases

DNASUi19258.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064091; ENSMUSP00000067614; ENSMUSG00000026384.
GeneIDi19258.
KEGGimmu:19258.
UCSCiuc007ciz.1. mouse.

Organism-specific databases

CTDi5775.
MGIiMGI:1099792. Ptpn4.

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007048.
HOVERGENiHBG008322.
InParanoidiQ9WU22.
KOiK18037.
OMAiHTCTDFW.
OrthoDBiEOG76HQ1B.
TreeFamiTF315900.

Miscellaneous databases

ChiTaRSiPtpn4. mouse.
NextBioi296108.
PROiQ9WU22.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PTPN4.
ExpressionAtlasiQ9WU22. baseline and differential.
GenevisibleiQ9WU22. MM.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000927. Tyr-Ptase_nr3. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a protein tyrosine phosphatase enriched in testis, a putative murine homologue of human PTPMEG."
    Park K.-W., Lee E.-J., Lee S.-H., Lee J.-E., Choi E.-Y., Kim B.J., Hwang R., Park K.A., Baik J.-H.
    Gene 257:45-55(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiPTN4_MOUSE
AccessioniPrimary (citable) accession number: Q9WU22
Secondary accession number(s): G5E8E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 18, 2013
Last modified: June 24, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.