Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein phosphatase non-receptor type 4

Gene

Ptpn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act at junctions between the membrane and the cytoskeleton.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei820SubstrateBy similarity1
Active sitei852Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei896SubstrateBy similarity1

GO - Molecular functioni

  • non-membrane spanning protein tyrosine phosphatase activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 4 (EC:3.1.3.48)
Alternative name(s):
Testis-enriched protein tyrosine phosphatase
Gene namesi
Name:Ptpn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1099792. Ptpn4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200751 – 926Tyrosine-protein phosphatase non-receptor type 4Add BLAST926

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei474PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WU22.
PeptideAtlasiQ9WU22.
PRIDEiQ9WU22.

PTM databases

iPTMnetiQ9WU22.
PhosphoSitePlusiQ9WU22.

Expressioni

Tissue specificityi

Highly expressed in testis. Specifically expressed in spermatocytes and spermatids within seminiferous tubules (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026384.
CleanExiMM_PTPN4.
ExpressionAtlasiQ9WU22. baseline and differential.
GenevisibleiQ9WU22. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd247P241613EBI-7249866,EBI-7803400

Protein-protein interaction databases

IntActiQ9WU22. 1 interactor.
MINTiMINT-126937.
STRINGi10090.ENSMUSP00000067614.

Structurei

3D structure databases

ProteinModelPortaliQ9WU22.
SMRiQ9WU22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 312FERMPROSITE-ProRule annotationAdd BLAST284
Domaini517 – 589PDZPROSITE-ProRule annotationAdd BLAST73
Domaini655 – 911Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST257

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni852 – 858Substrate bindingBy similarity7

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410KDUQ. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007048.
HOVERGENiHBG008322.
InParanoidiQ9WU22.
KOiK18037.
OMAiRKLMDWE.
OrthoDBiEOG091G016K.
TreeFamiTF315900.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000387. TYR_PHOSPHATASE_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000927. Tyr-Ptase_nr3. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WU22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFRVNKHDQ
60 70 80 90 100
GQVLLDIVFK HLDLTERDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP
110 120 130 140 150
YNLNFRVKFF VSDPNKLQEE YTRYQYFLQI KQDILTGRLS CPCNTAALLA
160 170 180 190 200
SFAVQSELGD YNQSENLAGY LSDYSFIPNQ PQDFEKEIAK LHQQHVGLSP
210 220 230 240 250
AEAEFNYLNA ARTLELYGVE FHYARDQSNN EILIGVMSGG ILIYKNRVRM
260 270 280 290 300
NTFLWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKTLWKA
310 320 330 340 350
CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN
360 370 380 390 400
KDRVFARSPS KPLARKLMDW EVVSRNSLSD DRLETQSLPS RSPPGTPNHR
410 420 430 440 450
NSSFTQEATR VRPSSVGHLV DHVVHMSPSE DFVSQRSPSS TQANSIVLES
460 470 480 490 500
SPSQETPEDG QPPALPPKQS KKNSWNQIHF SNSQQDLVTH TNESFDVPSS
510 520 530 540 550
PEKSTPNGGI PHDNLVLIKM KPDENGRFGF NVKGGYDQKM PVIVSRVAPG
560 570 580 590 600
TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC EKHSGELVLL
610 620 630 640 650
VRPNAVYDVV EEKLESEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL
660 670 680 690 700
ITGTVLAQFD QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVLLKG
710 720 730 740 750
NEDYINANYI NMEIPSSSII NQYIACQGPL PHTCKDFWQM TWEQGSSMVV
760 770 780 790 800
MLTTQVERGR VKCHQYWPEP SESSSYGCYQ VTCHSEEGNP AYIFRKMTLF
810 820 830 840 850
NQEKNESRQL TQIQYTAWPD HGVPDDSSDF LDFVCHVRDQ RAGKEEPIIV
860 870 880 890 900
HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ
910 920
YRFVCEAILK VYEEGFVKPL TTSSNK
Length:926
Mass (Da):105,832
Last modified:September 18, 2013 - v2
Checksum:i73E465D804C9C5FB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311D → G in AAD22773 (PubMed:11054567).Curated1
Sequence conflicti359P → S in AAD22773 (PubMed:11054567).Curated1
Sequence conflicti741T → I in AAD22773 (PubMed:11054567).Curated1
Sequence conflicti781V → A in AAD22773 (PubMed:11054567).Curated1
Sequence conflicti800F → I in AAD22773 (PubMed:11054567).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106702 mRNA. Translation: AAD22773.1.
AC123955 Genomic DNA. No translation available.
AC124760 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39812.1.
CCDSiCCDS15227.1.
RefSeqiNP_064317.2. NM_019933.2.
UniGeneiMm.458796.

Genome annotation databases

EnsembliENSMUST00000064091; ENSMUSP00000067614; ENSMUSG00000026384.
GeneIDi19258.
KEGGimmu:19258.
UCSCiuc007ciz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106702 mRNA. Translation: AAD22773.1.
AC123955 Genomic DNA. No translation available.
AC124760 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39812.1.
CCDSiCCDS15227.1.
RefSeqiNP_064317.2. NM_019933.2.
UniGeneiMm.458796.

3D structure databases

ProteinModelPortaliQ9WU22.
SMRiQ9WU22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WU22. 1 interactor.
MINTiMINT-126937.
STRINGi10090.ENSMUSP00000067614.

PTM databases

iPTMnetiQ9WU22.
PhosphoSitePlusiQ9WU22.

Proteomic databases

PaxDbiQ9WU22.
PeptideAtlasiQ9WU22.
PRIDEiQ9WU22.

Protocols and materials databases

DNASUi19258.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064091; ENSMUSP00000067614; ENSMUSG00000026384.
GeneIDi19258.
KEGGimmu:19258.
UCSCiuc007ciz.1. mouse.

Organism-specific databases

CTDi5775.
MGIiMGI:1099792. Ptpn4.

Phylogenomic databases

eggNOGiENOG410KDUQ. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007048.
HOVERGENiHBG008322.
InParanoidiQ9WU22.
KOiK18037.
OMAiRKLMDWE.
OrthoDBiEOG091G016K.
TreeFamiTF315900.

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.

Miscellaneous databases

ChiTaRSiPtpn4. mouse.
PROiQ9WU22.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026384.
CleanExiMM_PTPN4.
ExpressionAtlasiQ9WU22. baseline and differential.
GenevisibleiQ9WU22. MM.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000387. TYR_PHOSPHATASE_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000927. Tyr-Ptase_nr3. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTN4_MOUSE
AccessioniPrimary (citable) accession number: Q9WU22
Secondary accession number(s): G5E8E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 18, 2013
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.