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Q9WU20 (MTHR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:Mthfr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length654 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Enzyme regulation

Allosterically regulated by S-adenosylmethionine By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 654654Methylenetetrahydrofolate reductase
PRO_0000190247

Regions

Nucleotide binding62 – 676NAD By similarity
Nucleotide binding93 – 942NAD and FAD By similarity
Nucleotide binding156 – 1583FAD By similarity
Nucleotide binding173 – 1742FAD By similarity
Nucleotide binding200 – 2034FAD By similarity

Sites

Active site621Proton donor/acceptor By similarity
Binding site1261FAD By similarity
Binding site1581Substrate By similarity
Binding site1961FAD By similarity
Binding site2091FAD By similarity
Binding site2161FAD By similarity
Binding site2271Substrate By similarity
Binding site3201Substrate By similarity
Binding site3241Substrate By similarity

Experimental info

Sequence conflict3031S → R in AAD20313. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9WU20 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 96E625014D8F4838

FASTA65474,580
        10         20         30         40         50         60 
MVNEARGSGS PNPRSEGSSS GSESSKDSSR CSTPSLDPER HERLREKMRR RMDSGDKWFS 

        70         80         90        100        110        120 
LEFFPPRTAE GAVNLISRFD RMAAGGPLFV DVTWHPAGDP GSDKETSSMM IASTAVNYCG 

       130        140        150        160        170        180 
LETILHMTCC QQRPEEITGH LHRAKQLGLK NIMALRGDPV GDHWEAEEGG FSYATDLVKH 

       190        200        210        220        230        240 
IRTEFADYFD ICVAGYPRGH PDAESFEDDL KHLKEKVSAG ADFIITQLFF EASTFFSFVK 

       250        260        270        280        290        300 
ACTEIGISCP ILPGIFPIQG YTSLRQLVKL SKLEVPQKIK DVIEPIKDND AAIRNYGIEL 

       310        320        330        340        350        360 
AVSLCRELLD SGLVPGLHFY TLNREVATME VLKQLGMWTE DPRRPLPWAL SAHPKRREED 

       370        380        390        400        410        420 
VRPIFWASRP KSYIYRTQDW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPREELLKMW 

       430        440        450        460        470        480 
GEELTSEESV FEVFEHYLSG EPNRHGYRVT CLPWNDEPLA AETSLMKEEL LRVNRLGILT 

       490        500        510        520        530        540 
INSQPNINAK PSSDPVVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKT YELRVNYHIV 

       550        560        570        580        590        600 
DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPISFM FWKDEAFALW IEQWGKLYEE 

       610        620        630        640        650 
ESPSRMIIQY IHDNYFLVNL VDNEFPLDSC LWQVVEDTFE LLNRHPTERE TQAP 

« Hide

References

« Hide 'large scale' references
[1]"Gene structure of human and mouse methylenetetrahydrofolate reductase (MTHFR)."
Goyette P., Pai A., Milos R., Frosst P., Tran P., Chen Z., Chan M., Rozen R.
Mamm. Genome 9:652-656(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF105998 expand/collapse EMBL AC list , AF105988, AF105989, AF105990, AF105991, AF105992, AF105993, AF105994, AF105995, AF105996, AF105997 Genomic DNA. Translation: AAD20313.1.
AK030192 mRNA. Translation: BAC26832.1.
AL606929 Genomic DNA. Translation: CAM14896.1.
CH466594 Genomic DNA. Translation: EDL14794.1.
CH466594 Genomic DNA. Translation: EDL14796.1.
CH466594 Genomic DNA. Translation: EDL14797.1.
BC051017 mRNA. Translation: AAH51017.1.
BC052466 mRNA. Translation: AAH52466.1.
RefSeqNP_034970.2. NM_010840.3.
UniGeneMm.89959.

3D structure databases

ProteinModelPortalQ9WU20.
SMRQ9WU20. Positions 59-337.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9WU20.

Proteomic databases

PaxDbQ9WU20.
PRIDEQ9WU20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000097788; ENSMUSP00000095395; ENSMUSG00000029009.
GeneID17769.
KEGGmmu:17769.
UCSCuc008vtu.2. mouse.

Organism-specific databases

CTD4524.
MGIMGI:106639. Mthfr.

Phylogenomic databases

eggNOGCOG0685.
GeneTreeENSGT00390000012490.
HOGENOMHOG000246234.
HOVERGENHBG006414.
InParanoidQ8CDE4.
KOK00297.
OrthoDBEOG7TF7B4.

Enzyme and pathway databases

BRENDA1.5.1.15. 3474.
UniPathwayUPA00193.

Gene expression databases

ArrayExpressQ9WU20.
BgeeQ9WU20.
CleanExMM_MTHFR.

Family and domain databases

InterProIPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00677. fadh2_euk. 1 hit.
ProtoNetSearch...

Other

NextBio292481.
PROQ9WU20.
SOURCESearch...

Entry information

Entry nameMTHR_MOUSE
AccessionPrimary (citable) accession number: Q9WU20
Secondary accession number(s): Q8CDE4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: January 22, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot