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Q9WU20

- MTHR_MOUSE

UniProt

Q9WU20 - MTHR_MOUSE

Protein

Methylenetetrahydrofolate reductase

Gene

Mthfr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Allosterically regulated by S-adenosylmethionine.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei62 – 621Proton donor/acceptorBy similarity
    Binding sitei126 – 1261FADBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei196 – 1961FADBy similarity
    Binding sitei209 – 2091FADBy similarity
    Binding sitei216 – 2161FADBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Binding sitei320 – 3201SubstrateBy similarity
    Binding sitei324 – 3241SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 676NADBy similarity
    Nucleotide bindingi93 – 942NAD and FADBy similarity
    Nucleotide bindingi156 – 1583FADBy similarity
    Nucleotide bindingi173 – 1742FADBy similarity
    Nucleotide bindingi200 – 2034FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: MGI

    GO - Biological processi

    1. methionine biosynthetic process Source: MGI
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BRENDAi1.5.1.15. 3474.
    ReactomeiREACT_220137. Metabolism of folate and pterines.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:Mthfr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:106639. Mthfr.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 654654Methylenetetrahydrofolate reductasePRO_0000190247Add
    BLAST

    Proteomic databases

    PaxDbiQ9WU20.
    PRIDEiQ9WU20.

    PTM databases

    PhosphoSiteiQ9WU20.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9WU20.
    BgeeiQ9WU20.
    CleanExiMM_MTHFR.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WU20.
    SMRiQ9WU20. Positions 59-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    GeneTreeiENSGT00390000012490.
    HOGENOMiHOG000246234.
    HOVERGENiHBG006414.
    InParanoidiQ8CDE4.
    KOiK00297.
    OrthoDBiEOG7TF7B4.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WU20-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNEARGSGS PNPRSEGSSS GSESSKDSSR CSTPSLDPER HERLREKMRR    50
    RMDSGDKWFS LEFFPPRTAE GAVNLISRFD RMAAGGPLFV DVTWHPAGDP 100
    GSDKETSSMM IASTAVNYCG LETILHMTCC QQRPEEITGH LHRAKQLGLK 150
    NIMALRGDPV GDHWEAEEGG FSYATDLVKH IRTEFADYFD ICVAGYPRGH 200
    PDAESFEDDL KHLKEKVSAG ADFIITQLFF EASTFFSFVK ACTEIGISCP 250
    ILPGIFPIQG YTSLRQLVKL SKLEVPQKIK DVIEPIKDND AAIRNYGIEL 300
    AVSLCRELLD SGLVPGLHFY TLNREVATME VLKQLGMWTE DPRRPLPWAL 350
    SAHPKRREED VRPIFWASRP KSYIYRTQDW DEFPNGRWGN SSSPAFGELK 400
    DYYLFYLKSK SPREELLKMW GEELTSEESV FEVFEHYLSG EPNRHGYRVT 450
    CLPWNDEPLA AETSLMKEEL LRVNRLGILT INSQPNINAK PSSDPVVGWG 500
    PSGGYVFQKA YLEFFTSRET VEALLQVLKT YELRVNYHIV DVKGENITNA 550
    PELQPNAVTW GIFPGREIIQ PTVVDPISFM FWKDEAFALW IEQWGKLYEE 600
    ESPSRMIIQY IHDNYFLVNL VDNEFPLDSC LWQVVEDTFE LLNRHPTERE 650
    TQAP 654
    Length:654
    Mass (Da):74,580
    Last modified:July 27, 2011 - v2
    Checksum:i96E625014D8F4838
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti303 – 3031S → R in AAD20313. (PubMed:9680386)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF105998
    , AF105988, AF105989, AF105990, AF105991, AF105992, AF105993, AF105994, AF105995, AF105996, AF105997 Genomic DNA. Translation: AAD20313.1.
    AK030192 mRNA. Translation: BAC26832.1.
    AL606929 Genomic DNA. Translation: CAM14896.1.
    CH466594 Genomic DNA. Translation: EDL14794.1.
    CH466594 Genomic DNA. Translation: EDL14796.1.
    CH466594 Genomic DNA. Translation: EDL14797.1.
    BC051017 mRNA. Translation: AAH51017.1.
    BC052466 mRNA. Translation: AAH52466.1.
    CCDSiCCDS18929.1.
    RefSeqiNP_034970.2. NM_010840.3.
    UniGeneiMm.89959.

    Genome annotation databases

    EnsembliENSMUST00000097788; ENSMUSP00000095395; ENSMUSG00000029009.
    GeneIDi17769.
    KEGGimmu:17769.
    UCSCiuc008vtu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF105998
    , AF105988 , AF105989 , AF105990 , AF105991 , AF105992 , AF105993 , AF105994 , AF105995 , AF105996 , AF105997 Genomic DNA. Translation: AAD20313.1 .
    AK030192 mRNA. Translation: BAC26832.1 .
    AL606929 Genomic DNA. Translation: CAM14896.1 .
    CH466594 Genomic DNA. Translation: EDL14794.1 .
    CH466594 Genomic DNA. Translation: EDL14796.1 .
    CH466594 Genomic DNA. Translation: EDL14797.1 .
    BC051017 mRNA. Translation: AAH51017.1 .
    BC052466 mRNA. Translation: AAH52466.1 .
    CCDSi CCDS18929.1.
    RefSeqi NP_034970.2. NM_010840.3.
    UniGenei Mm.89959.

    3D structure databases

    ProteinModelPortali Q9WU20.
    SMRi Q9WU20. Positions 59-337.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9WU20.

    Proteomic databases

    PaxDbi Q9WU20.
    PRIDEi Q9WU20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000097788 ; ENSMUSP00000095395 ; ENSMUSG00000029009 .
    GeneIDi 17769.
    KEGGi mmu:17769.
    UCSCi uc008vtu.2. mouse.

    Organism-specific databases

    CTDi 4524.
    MGIi MGI:106639. Mthfr.

    Phylogenomic databases

    eggNOGi COG0685.
    GeneTreei ENSGT00390000012490.
    HOGENOMi HOG000246234.
    HOVERGENi HBG006414.
    InParanoidi Q8CDE4.
    KOi K00297.
    OrthoDBi EOG7TF7B4.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BRENDAi 1.5.1.15. 3474.
    Reactomei REACT_220137. Metabolism of folate and pterines.

    Miscellaneous databases

    NextBioi 292481.
    PROi Q9WU20.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WU20.
    Bgeei Q9WU20.
    CleanExi MM_MTHFR.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure of human and mouse methylenetetrahydrofolate reductase (MTHFR)."
      Goyette P., Pai A., Milos R., Frosst P., Tran P., Chen Z., Chan M., Rozen R.
      Mamm. Genome 9:652-656(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Olfactory epithelium.

    Entry informationi

    Entry nameiMTHR_MOUSE
    AccessioniPrimary (citable) accession number: Q9WU20
    Secondary accession number(s): Q8CDE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3