Reviewed, UniProtKB/Swiss-Prot Q9WU20 (MTHR_MOUSE)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:

Mthfr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.
Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Allosterically regulated by S-adenosylmethionine By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 654	654	Methylenetetrahydrofolate reductase		PRO_0000190247

Sequences

	Sequence		Length	Mass (Da)
	Q9WU20-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 12AD31B06B371E17	FASTA	654	74,649
	10 20 30 40 50 60 MVNEARGSGS PNPRSEGSSS GSESSKDSSR CSTPSLDPER HERLREKMRR RMDSGDKWFS 70 80 90 100 110 120 LEFFPPRTAE GAVNLISRFD RMAAGGPLFV DVTWHPAGDP GSDKETSSMM IASTAVNYCG 130 140 150 160 170 180 LETILHMTCC QQRPEEITGH LHRAKQLGLK NIMALRGDPV GDHWEAEEGG FSYATDLVKH 190 200 210 220 230 240 IRTEFADYFD ICVAGYPRGH PDAESFEDDL KHLKEKVSAG ADFIITQLFF EASTFFSFVK 250 260 270 280 290 300 ACTEIGISCP ILPGIFPIQG YTSLRQLVKL SKLEVPQKIK DVIEPIKDND AAIRNYGIEL 310 320 330 340 350 360 AVRLCRELLD SGLVPGLHFY TLNREVATME VLKQLGMWTE DPRRPLPWAL SAHPKRREED 370 380 390 400 410 420 VRPIFWASRP KSYIYRTQDW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPREELLKMW 430 440 450 460 470 480 GEELTSEESV FEVFEHYLSG EPNRHGYRVT CLPWNDEPLA AETSLMKEEL LRVNRLGILT 490 500 510 520 530 540 INSQPNINAK PSSDPVVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKT YELRVNYHIV 550 560 570 580 590 600 DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPISFM FWKDEAFALW IEQWGKLYEE 610 620 630 640 650 ESPSRMIIQY IHDNYFLVNL VDNEFPLDSC LWQVVEDTFE LLNRHPTERE TQAP

References

[1]	"Gene structure of human and mouse methylenetetrahydrofolate reductase (MTHFR)." Goyette P., Pai A., Milos R., Frosst P., Tran P., Chen Z., Chan M., Rozen R. Mamm. Genome 9:652-656(1998) [PubMed: 9680386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF105998 , AF105988, AF105989, AF105990, AF105991, AF105992, AF105993, AF105994, AF105995, AF105996, AF105997 Genomic DNA. Translation: AAD20313.1.
IPI	IPI00880907.
UniGene	Mm.89959
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WU20.
PTM databases
PhosphoSite	Q9WU20.
Proteomic databases
PRIDE	Q9WU20.
Genome annotation databases
Ensembl	ENSMUST00000069604; ENSMUSP00000069774; ENSMUSG00000029009; Mus musculus. [Genome view] ENSMUST00000097788; ENSMUSP00000095395; ENSMUSG00000029009; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:106639. Mthfr.
Phylogenomic databases
HOVERGEN	Q9WU20.
Enzyme and pathway databases
BRENDA	1.5.1.15. 244. 1.5.1.20. 244.
Gene expression databases
ArrayExpress	Q9WU20.
Bgee	Q9WU20.
CleanEx	MM_MTHFR.
Genevestigator	Q9WU20.
GermOnline	ENSMUSG00000029009. Mus musculus.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

MTHR_MOUSE

Accession

Primary (citable) accession number: Q9WU20

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)