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Protein

Hydroxyacid oxidase 1

Gene

Hao1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate (By similarity).By similarity1 Publication

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

Pathwayi: glycolate degradation

This protein is involved in step 1 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Hydroxyacid oxidase 1 (Hao1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstratePROSITE-ProRule annotation
Binding sitei108 – 1081FMNPROSITE-ProRule annotation
Binding sitei130 – 1301FMNPROSITE-ProRule annotation
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei158 – 1581FMNPROSITE-ProRule annotation
Binding sitei167 – 1671SubstratePROSITE-ProRule annotation
Binding sitei236 – 2361FMNPROSITE-ProRule annotation
Active sitei260 – 2601Proton acceptorPROSITE-ProRule annotation
Binding sitei263 – 2631SubstratePROSITE-ProRule annotation
Binding sitei315 – 3151FMNPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 31525FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

ReactomeiR-MMU-389661. Glyoxylate metabolism and glycine degradation.
UniPathwayiUPA00864; UER00830.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacid oxidase 1 (EC:1.1.3.15)
Short name:
HAOX1
Alternative name(s):
Glycolate oxidase
Short name:
GOX
Gene namesi
Name:Hao1
Synonyms:Gox1, Hao-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96011. Hao1.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Hydroxyacid oxidase 1PRO_0000206319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841N6-succinyllysineCombined sources
Modified residuei194 – 1941PhosphoserineCombined sources
Modified residuei230 – 2301PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WU19.
PaxDbiQ9WU19.
PRIDEiQ9WU19.

PTM databases

iPTMnetiQ9WU19.
PhosphoSiteiQ9WU19.

Expressioni

Tissue specificityi

Liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000027261.
CleanExiMM_HAO1.
ExpressionAtlasiQ9WU19. baseline and differential.
GenevisibleiQ9WU19. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WU19. 2 interactions.
MINTiMINT-1848223.
STRINGi10090.ENSMUSP00000028704.

Structurei

3D structure databases

ProteinModelPortaliQ9WU19.
SMRiQ9WU19. Positions 3-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 365365FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi368 – 3703Microbody targeting signalSequence analysis

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
GeneTreeiENSGT00390000018717.
HOGENOMiHOG000217463.
HOVERGENiHBG051881.
InParanoidiQ9WU19.
KOiK11517.
OMAiQKSVYDY.
OrthoDBiEOG091G0ADA.
PhylomeDBiQ9WU19.
TreeFamiTF313363.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WU19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY
60 70 80 90 100
PRMLRNVADI DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT
110 120 130 140 150
MGTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ
160 170 180 190 200
GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG
210 220 230 240 250
DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR GDDAKEAVKH
260 270 280 290 300
GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV
310 320 330 340 350
LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ
360 370
NVKVIDKTLV RKNPLAVSKI
Length:370
Mass (Da):41,001
Last modified:November 1, 1999 - v1
Checksum:i97339211AF9FC19C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104312 mRNA. Translation: AAD25332.1.
CCDSiCCDS16783.1.
RefSeqiNP_034533.1. NM_010403.2.
UniGeneiMm.26634.

Genome annotation databases

EnsembliENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261.
GeneIDi15112.
KEGGimmu:15112.
UCSCiuc008mns.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104312 mRNA. Translation: AAD25332.1.
CCDSiCCDS16783.1.
RefSeqiNP_034533.1. NM_010403.2.
UniGeneiMm.26634.

3D structure databases

ProteinModelPortaliQ9WU19.
SMRiQ9WU19. Positions 3-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WU19. 2 interactions.
MINTiMINT-1848223.
STRINGi10090.ENSMUSP00000028704.

PTM databases

iPTMnetiQ9WU19.
PhosphoSiteiQ9WU19.

Proteomic databases

MaxQBiQ9WU19.
PaxDbiQ9WU19.
PRIDEiQ9WU19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261.
GeneIDi15112.
KEGGimmu:15112.
UCSCiuc008mns.1. mouse.

Organism-specific databases

CTDi54363.
MGIiMGI:96011. Hao1.

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
GeneTreeiENSGT00390000018717.
HOGENOMiHOG000217463.
HOVERGENiHBG051881.
InParanoidiQ9WU19.
KOiK11517.
OMAiQKSVYDY.
OrthoDBiEOG091G0ADA.
PhylomeDBiQ9WU19.
TreeFamiTF313363.

Enzyme and pathway databases

UniPathwayiUPA00864; UER00830.
ReactomeiR-MMU-389661. Glyoxylate metabolism and glycine degradation.

Miscellaneous databases

PROiQ9WU19.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027261.
CleanExiMM_HAO1.
ExpressionAtlasiQ9WU19. baseline and differential.
GenevisibleiQ9WU19. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHAOX1_MOUSE
AccessioniPrimary (citable) accession number: Q9WU19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.