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Q9WU19 (HAOX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyacid oxidase 1
Short name=HAOX1
EC=1.1.3.15
Alternative name(s):
Glycolate oxidase
Short name=GOX
Gene names
Name:Hao1
Synonyms:Gox1, Hao-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate By similarity. Ref.1

Catalytic activity

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactor

FMN.

Pathway

Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4.

Subcellular location

Peroxisome.

Tissue specificity

Liver. Ref.1

Sequence similarities

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Contains 1 FMN hydroxy acid dehydrogenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Hydroxyacid oxidase 1
PRO_0000206319

Regions

Domain1 – 365365FMN hydroxy acid dehydrogenase
Nucleotide binding291 – 31525FMN By similarity
Motif368 – 3703Microbody targeting signal Potential

Sites

Active site2601Proton acceptor By similarity
Binding site261Substrate Potential
Binding site1081FMN By similarity
Binding site1301FMN By similarity
Binding site1321Substrate By similarity
Binding site1581FMN By similarity
Binding site1671Substrate By similarity
Binding site2361FMN By similarity
Binding site2631Substrate Potential
Binding site3151FMN By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9WU19 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 97339211AF9FC19C

FASTA37041,001
        10         20         30         40         50         60 
MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY PRMLRNVADI 

        70         80         90        100        110        120 
DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT MGTGMMLSSW ATSSIEEVAE 

       130        140        150        160        170        180 
AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ 

       190        200        210        220        230        240 
LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR 

       250        260        270        280        290        300 
GDDAKEAVKH GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV 

       310        320        330        340        350        360 
LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ NVKVIDKTLV 

       370 
RKNPLAVSKI

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References

[1]"Molecular cloning of mouse glycolate oxidase. High evolutionary conservation and presence of an iron-responsive element-like sequence in the mRNA."
Kohler S.A., Menotti E., Kuhn L.C.
J. Biol. Chem. 274:2401-2407(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF104312 mRNA. Translation: AAD25332.1.
IPIIPI00123750.
RefSeqNP_034533.1. NM_010403.2.
UniGeneMm.26634.

3D structure databases

ProteinModelPortalQ9WU19.
SMRQ9WU19. Positions 3-362.
ModBaseSearch...

PTM databases

PhosphoSiteQ9WU19.

Proteomic databases

PaxDbQ9WU19.
PRIDEQ9WU19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261.
GeneID15112.
KEGGmmu:15112.

Organism-specific databases

CTD54363.
MGIMGI:96011. Hao1.

Phylogenomic databases

eggNOGCOG1304.
GeneTreeENSGT00390000018717.
HOGENOMHOG000217463.
HOVERGENHBG051881.
InParanoidQ9WU19.
KOK11517.
OMAESPTMST.
OrthoDBEOG4MGS7T.

Enzyme and pathway databases

UniPathwayUPA00864; UER00830.

Gene expression databases

ArrayExpressQ9WU19.
BgeeQ9WU19.
CleanExMM_HAO1.
GenevestigatorQ9WU19.
GermOnlineENSMUSG00000027261. Mus musculus.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHAO1. mouse.
NextBio287518.
SOURCESearch...

Entry information

Entry nameHAOX1_MOUSE
AccessionPrimary (citable) accession number: Q9WU19
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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