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Q9WU19

- HAOX1_MOUSE

UniProt

Q9WU19 - HAOX1_MOUSE

Protein

Hydroxyacid oxidase 1

Gene

Hao1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate By similarity.By similarity

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei26 – 261SubstratePROSITE-ProRule annotation
    Binding sitei108 – 1081FMNPROSITE-ProRule annotation
    Binding sitei130 – 1301FMNPROSITE-ProRule annotation
    Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
    Binding sitei158 – 1581FMNPROSITE-ProRule annotation
    Binding sitei167 – 1671SubstratePROSITE-ProRule annotation
    Binding sitei236 – 2361FMNPROSITE-ProRule annotation
    Active sitei260 – 2601Proton acceptorPROSITE-ProRule annotation
    Binding sitei263 – 2631SubstratePROSITE-ProRule annotation
    Binding sitei315 – 3151FMNPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi291 – 31525FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. (S)-2-hydroxy-acid oxidase activity Source: UniProtKB
    2. FMN binding Source: UniProtKB
    3. glycolate oxidase activity Source: UniProtKB
    4. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    5. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    6. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid alpha-oxidation Source: UniProtKB
    2. glycolate catabolic process Source: UniProtKB
    3. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    UniPathwayiUPA00864; UER00830.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyacid oxidase 1 (EC:1.1.3.15)
    Short name:
    HAOX1
    Alternative name(s):
    Glycolate oxidase
    Short name:
    GOX
    Gene namesi
    Name:Hao1
    Synonyms:Gox1, Hao-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:96011. Hao1.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: HGNC

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Hydroxyacid oxidase 1PRO_0000206319Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei184 – 1841N6-succinyllysine1 Publication
    Modified residuei194 – 1941Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9WU19.
    PaxDbiQ9WU19.
    PRIDEiQ9WU19.

    PTM databases

    PhosphoSiteiQ9WU19.

    Expressioni

    Tissue specificityi

    Liver.1 Publication

    Gene expression databases

    BgeeiQ9WU19.
    CleanExiMM_HAO1.
    GenevestigatoriQ9WU19.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9WU19. 2 interactions.
    MINTiMINT-1848223.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WU19.
    SMRiQ9WU19. Positions 3-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 365365FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi368 – 3703Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    GeneTreeiENSGT00390000018717.
    HOGENOMiHOG000217463.
    HOVERGENiHBG051881.
    InParanoidiQ9WU19.
    KOiK11517.
    OMAiSPTENFG.
    OrthoDBiEOG7B5WW0.
    PhylomeDBiQ9WU19.
    TreeFamiTF313363.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WU19-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY    50
    PRMLRNVADI DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT 100
    MGTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ 150
    GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG 200
    DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR GDDAKEAVKH 250
    GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV 300
    LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ 350
    NVKVIDKTLV RKNPLAVSKI 370
    Length:370
    Mass (Da):41,001
    Last modified:November 1, 1999 - v1
    Checksum:i97339211AF9FC19C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104312 mRNA. Translation: AAD25332.1.
    CCDSiCCDS16783.1.
    RefSeqiNP_034533.1. NM_010403.2.
    UniGeneiMm.26634.

    Genome annotation databases

    EnsembliENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261.
    GeneIDi15112.
    KEGGimmu:15112.
    UCSCiuc008mns.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104312 mRNA. Translation: AAD25332.1 .
    CCDSi CCDS16783.1.
    RefSeqi NP_034533.1. NM_010403.2.
    UniGenei Mm.26634.

    3D structure databases

    ProteinModelPortali Q9WU19.
    SMRi Q9WU19. Positions 3-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WU19. 2 interactions.
    MINTi MINT-1848223.

    PTM databases

    PhosphoSitei Q9WU19.

    Proteomic databases

    MaxQBi Q9WU19.
    PaxDbi Q9WU19.
    PRIDEi Q9WU19.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028704 ; ENSMUSP00000028704 ; ENSMUSG00000027261 .
    GeneIDi 15112.
    KEGGi mmu:15112.
    UCSCi uc008mns.1. mouse.

    Organism-specific databases

    CTDi 54363.
    MGIi MGI:96011. Hao1.

    Phylogenomic databases

    eggNOGi COG1304.
    GeneTreei ENSGT00390000018717.
    HOGENOMi HOG000217463.
    HOVERGENi HBG051881.
    InParanoidi Q9WU19.
    KOi K11517.
    OMAi SPTENFG.
    OrthoDBi EOG7B5WW0.
    PhylomeDBi Q9WU19.
    TreeFami TF313363.

    Enzyme and pathway databases

    UniPathwayi UPA00864 ; UER00830 .

    Miscellaneous databases

    ChiTaRSi HAO1. mouse.
    NextBioi 287518.
    PROi Q9WU19.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WU19.
    CleanExi MM_HAO1.
    Genevestigatori Q9WU19.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of mouse glycolate oxidase. High evolutionary conservation and presence of an iron-responsive element-like sequence in the mRNA."
      Kohler S.A., Menotti E., Kuhn L.C.
      J. Biol. Chem. 274:2401-2407(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiHAOX1_MOUSE
    AccessioniPrimary (citable) accession number: Q9WU19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3