Reviewed, UniProtKB/Swiss-Prot Q9WU19 (HAOX1_MOUSE)

Names and origin

Protein names

Recommended name:
    Hydroxyacid oxidase 1
      Short name=HAOX1
    EC=1.1.3.15
Alternative name(s):
    Glycolate oxidase
      Short name=GOX

Gene names

Name:	Hao1
Synonyms:	Gox1, Hao-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.
Catalytic activity	(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.
Cofactor	FMN.
Pathway	Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.
Subcellular location	Peroxisome.
Tissue specificity	Liver.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from direct assay. Source: HGNC
Molecular function	(S)-2-hydroxy-acid oxidase activity Inferred from direct assay. Source: MGI FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Hydroxyacid oxidase 1		PRO_0000206319
Regions
	Domain	1 – 365	365	FMN hydroxy acid dehydrogenase
	Nucleotide binding	291 – 315	25	FMN By similarity
	Motif	368 – 370	3	Microbody targeting signal Potential
Sites
	Active site	260	1	Proton acceptor By similarity
	Binding site	26	1	Substrate Potential
	Binding site	108	1	FMN By similarity
	Binding site	130	1	FMN By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	158	1	FMN By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	236	1	FMN By similarity
	Binding site	263	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9WU19-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 97339211AF9FC19C	FASTA	370	41,001
	10 20 30 40 50 60 MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY PRMLRNVADI 70 80 90 100 110 120 DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT MGTGMMLSSW ATSSIEEVAE 130 140 150 160 170 180 AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ 190 200 210 220 230 240 LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR 250 260 270 280 290 300 GDDAKEAVKH GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV 310 320 330 340 350 360 LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ NVKVIDKTLV 370 RKNPLAVSKI

References

[1]	"Molecular cloning of mouse glycolate oxidase. High evolutionary conservation and presence of an iron-responsive element-like sequence in the mRNA." Kohler S.A., Menotti E., Kuhn L.C. J. Biol. Chem. 274:2401-2407(1999) [PubMed: 9891009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF104312 mRNA. Translation: AAD25332.1.
IPI	IPI00123750.
RefSeq	NP_034533.1.
UniGene	Mm.26634
3D structure databases
HSSP	HSSP built from PDB template 1AL8 based on UniProtKB P05414.
SMR	Q9WU19. Positions 4-361.
ModBase	Search...
PTM databases
PhosphoSite	Q9WU19.
Genome annotation databases
Ensembl	ENSMUSG00000027261. Mus musculus. [Contig view]
GeneID	15112.
KEGG	mmu:15112.
NMPDR	fig|10090.3.peg.6979.
Organism-specific databases
MGI	MGI:96011. Hao1.
Phylogenomic databases
HOGENOM	Q9WU19.
HOVERGEN	Q9WU19.
OMA	Q9WU19. IDRSLSW.
Enzyme and pathway databases
BRENDA	1.1.3.15. 244.
Gene expression databases
ArrayExpress	Q9WU19.
Bgee	Q9WU19.
CleanEx	MM_HAO1.
GermOnline	ENSMUSG00000027261. Mus musculus.
Family and domain databases
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR017934. FMN-dep_OHA_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287518.
SOURCE	Search...

Entry information

Entry name

HAOX1_MOUSE

Accession

Primary (citable) accession number: Q9WU19

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)