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Protein

Hydroxyacid oxidase 1

Gene

Hao1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate (By similarity).By similarity1 Publication

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

Pathwayi: glycolate degradation

This protein is involved in step 1 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Hydroxyacid oxidase 1 (Hao1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26SubstratePROSITE-ProRule annotation1
Binding sitei108FMNPROSITE-ProRule annotation1
Binding sitei130FMNPROSITE-ProRule annotation1
Binding sitei132SubstratePROSITE-ProRule annotation1
Binding sitei158FMNPROSITE-ProRule annotation1
Binding sitei167SubstratePROSITE-ProRule annotation1
Binding sitei236FMNPROSITE-ProRule annotation1
Active sitei260Proton acceptorPROSITE-ProRule annotation1
Binding sitei263SubstratePROSITE-ProRule annotation1
Binding sitei315FMNPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi291 – 315FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN

Enzyme and pathway databases

ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation
R-MMU-9033241 Peroxisomal protein import
UniPathwayiUPA00864; UER00830

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacid oxidase 1 (EC:1.1.3.15)
Short name:
HAOX1
Alternative name(s):
Glycolate oxidase
Short name:
GOX
Gene namesi
Name:Hao1
Synonyms:Gox1, Hao-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96011 Hao1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002063191 – 370Hydroxyacid oxidase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184N6-succinyllysineCombined sources1
Modified residuei194PhosphoserineCombined sources1
Modified residuei230PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WU19
PaxDbiQ9WU19
PRIDEiQ9WU19

PTM databases

iPTMnetiQ9WU19
PhosphoSitePlusiQ9WU19
SwissPalmiQ9WU19

Expressioni

Tissue specificityi

Liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000027261
CleanExiMM_HAO1
ExpressionAtlasiQ9WU19 baseline and differential
GenevisibleiQ9WU19 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WU19, 2 interactors
MINTiQ9WU19
STRINGi10090.ENSMUSP00000028704

Structurei

3D structure databases

ProteinModelPortaliQ9WU19
SMRiQ9WU19
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 365FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST365

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi368 – 370Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538 Eukaryota
COG1304 LUCA
GeneTreeiENSGT00390000018717
HOGENOMiHOG000217463
HOVERGENiHBG051881
InParanoidiQ9WU19
KOiK11517
OMAiMQLYIYK
OrthoDBiEOG091G0ADA
PhylomeDBiQ9WU19
TreeFamiTF313363

Family and domain databases

CDDicd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012133 Alpha-hydoxy_acid_DH_FMN
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
PfamiView protein in Pfam
PF01070 FMN_dh, 1 hit
PIRSFiPIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEiView protein in PROSITE
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WU19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY
60 70 80 90 100
PRMLRNVADI DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT
110 120 130 140 150
MGTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ
160 170 180 190 200
GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG
210 220 230 240 250
DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR GDDAKEAVKH
260 270 280 290 300
GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV
310 320 330 340 350
LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ
360 370
NVKVIDKTLV RKNPLAVSKI
Length:370
Mass (Da):41,001
Last modified:November 1, 1999 - v1
Checksum:i97339211AF9FC19C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104312 mRNA Translation: AAD25332.1
CCDSiCCDS16783.1
RefSeqiNP_034533.1, NM_010403.2
UniGeneiMm.26634

Genome annotation databases

EnsembliENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261
GeneIDi15112
KEGGimmu:15112
UCSCiuc008mns.1 mouse

Similar proteinsi

Entry informationi

Entry nameiHAOX1_MOUSE
AccessioniPrimary (citable) accession number: Q9WU19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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