Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kunitz-type protease inhibitor 2

Gene

Spint2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 492Reactive bondBy similarity
Sitei143 – 1442Reactive bondBy similarity

GO - Molecular functioni

GO - Biological processi

  • basement membrane organization Source: MGI
  • cellular response to BMP stimulus Source: Ensembl
  • epithelial cell morphogenesis involved in placental branching Source: MGI
  • establishment or maintenance of cell polarity Source: MGI
  • negative regulation of cell-cell adhesion Source: MGI
  • negative regulation of cell motility Source: MGI
  • negative regulation of endopeptidase activity Source: MGI
  • negative regulation of neural precursor cell proliferation Source: Ensembl
  • neural tube closure Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 2
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 2
Short name:
HAI-2
Gene namesi
Name:Spint2
Synonyms:Hai2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1338031. Spint2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 197170ExtracellularSequence analysisAdd
BLAST
Transmembranei198 – 21821HelicalSequence analysisAdd
BLAST
Topological domaini219 – 25234CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 252225Kunitz-type protease inhibitor 2PRO_0000016886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 88PROSITE-ProRule annotation
Disulfide bondi47 ↔ 71PROSITE-ProRule annotation
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi63 ↔ 84PROSITE-ProRule annotation
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence analysis
Disulfide bondi133 ↔ 183PROSITE-ProRule annotation
Disulfide bondi142 ↔ 166PROSITE-ProRule annotation
Disulfide bondi158 ↔ 179PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9WU03.
PaxDbiQ9WU03.
PRIDEiQ9WU03.

PTM databases

iPTMnetiQ9WU03.
PhosphoSiteiQ9WU03.
SwissPalmiQ9WU03.

Expressioni

Tissue specificityi

Isoform 2 is more predominantly expressed than isoform 1.

Gene expression databases

BgeeiQ9WU03.
CleanExiMM_SPINT2.
GenevisibleiQ9WU03. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096204.

Structurei

3D structure databases

ProteinModelPortaliQ9WU03.
SMRiQ9WU03. Positions 35-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 8851BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 18351BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd
BLAST

Domaini

This inhibitor contains two inhibitory domains.

Sequence similaritiesi

Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000013109.
HOVERGENiHBG079207.
InParanoidiQ9WU03.
OMAiEEYCTAK.
OrthoDBiEOG74TX0C.
PhylomeDBiQ9WU03.
TreeFamiTF326553.

Family and domain databases

Gene3Di4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU03-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQLCELRRG RALLALVASL LLSGAQVASR ELDVHESCGV SKVVGKCRAS
60 70 80 90 100
IPRWWYNITD GSCQPFVYGG CEGNGNNYQS KEECLDKCAG VTENTTDDMA
110 120 130 140 150
RNRNGADSSV LSVPRKQSAE DLSAEIFNYE EYCVPKAVTG PCRAAFPRWY
160 170 180 190 200
YDTEKNSCIS FIYGGCRGNK NSYLSQEACM QHCSGKQMHP FLTPGLKAVI
210 220 230 240 250
LVGLFLMVLI LLLGTSMVCL IRVVRRKQER ALRTVWSTAD DKEQLVKNTC

VL
Length:252
Mass (Da):27,914
Last modified:November 1, 1999 - v1
Checksum:iB2FF4B86924D4F8F
GO
Isoform 2 (identifier: Q9WU03-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-93: Missing.

Show »
Length:195
Mass (Da):21,736
Checksum:iEF49C83AB4E3EDE0
GO
Isoform 3 (identifier: Q9WU03-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-93: Missing.
     114-128: PRKQSAEDLSAEIFN → CFVELSVAALFLFYA
     129-252: Missing.

Show »
Length:71
Mass (Da):7,671
Checksum:iFD28235C68C5692D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei37 – 9357Missing in isoform 2 and isoform 3. 1 PublicationVSP_003034Add
BLAST
Alternative sequencei114 – 12815PRKQS…AEIFN → CFVELSVAALFLFYA in isoform 3. CuratedVSP_003035Add
BLAST
Alternative sequencei129 – 252124Missing in isoform 3. CuratedVSP_003036Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099016 mRNA. Translation: AAD22172.1.
AF099019 mRNA. Translation: AAD22173.1.
AF099020 mRNA. Translation: AAD22174.1.
BC003431 mRNA. Translation: AAH03431.1.
BC026419 mRNA. Translation: AAH26419.1.
CCDSiCCDS39869.1. [Q9WU03-2]
CCDS39870.1. [Q9WU03-1]
PIRiJG0185.
RefSeqiNP_001076017.1. NM_001082548.1. [Q9WU03-2]
NP_035594.1. NM_011464.2. [Q9WU03-1]
UniGeneiMm.295230.

Genome annotation databases

EnsembliENSMUST00000098604; ENSMUSP00000096204; ENSMUSG00000074227. [Q9WU03-1]
ENSMUST00000108236; ENSMUSP00000103871; ENSMUSG00000074227. [Q9WU03-2]
ENSMUST00000207601; ENSMUSP00000146580; ENSMUSG00000074227. [Q9WU03-3]
GeneIDi20733.
KEGGimmu:20733.
UCSCiuc009gbk.1. mouse. [Q9WU03-1]
uc009gbl.1. mouse. [Q9WU03-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099016 mRNA. Translation: AAD22172.1.
AF099019 mRNA. Translation: AAD22173.1.
AF099020 mRNA. Translation: AAD22174.1.
BC003431 mRNA. Translation: AAH03431.1.
BC026419 mRNA. Translation: AAH26419.1.
CCDSiCCDS39869.1. [Q9WU03-2]
CCDS39870.1. [Q9WU03-1]
PIRiJG0185.
RefSeqiNP_001076017.1. NM_001082548.1. [Q9WU03-2]
NP_035594.1. NM_011464.2. [Q9WU03-1]
UniGeneiMm.295230.

3D structure databases

ProteinModelPortaliQ9WU03.
SMRiQ9WU03. Positions 35-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096204.

Protein family/group databases

MEROPSiI02.009.

PTM databases

iPTMnetiQ9WU03.
PhosphoSiteiQ9WU03.
SwissPalmiQ9WU03.

Proteomic databases

EPDiQ9WU03.
PaxDbiQ9WU03.
PRIDEiQ9WU03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098604; ENSMUSP00000096204; ENSMUSG00000074227. [Q9WU03-1]
ENSMUST00000108236; ENSMUSP00000103871; ENSMUSG00000074227. [Q9WU03-2]
ENSMUST00000207601; ENSMUSP00000146580; ENSMUSG00000074227. [Q9WU03-3]
GeneIDi20733.
KEGGimmu:20733.
UCSCiuc009gbk.1. mouse. [Q9WU03-1]
uc009gbl.1. mouse. [Q9WU03-2]

Organism-specific databases

CTDi10653.
MGIiMGI:1338031. Spint2.

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000013109.
HOVERGENiHBG079207.
InParanoidiQ9WU03.
OMAiEEYCTAK.
OrthoDBiEOG74TX0C.
PhylomeDBiQ9WU03.
TreeFamiTF326553.

Miscellaneous databases

ChiTaRSiSpint2. mouse.
PROiQ9WU03.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WU03.
CleanExiMM_SPINT2.
GenevisibleiQ9WU03. MM.

Family and domain databases

Gene3Di4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hepatocyte growth factor activator inhibitor type 2 lacking the first Kunitz-type serine proteinase inhibitor domain is a predominant product in mouse but not in human."
    Itoh H., Kataoka H., Hamasuna R., Kitamura N., Koono M.
    Biochem. Biophys. Res. Commun. 255:740-748(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: BALB/cJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II and FVB/N.
    Tissue: Colon and Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Lung, Pancreas and Testis.

Entry informationi

Entry nameiSPIT2_MOUSE
AccessioniPrimary (citable) accession number: Q9WU03
Secondary accession number(s): Q5D0F2, Q9WU04, Q9WU05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.