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Protein

Kunitz-type protease inhibitor 2

Gene

Spint2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei48 – 49Reactive bondBy similarity2
Sitei143 – 144Reactive bondBy similarity2

GO - Molecular functioni

GO - Biological processi

  • basement membrane organization Source: MGI
  • cellular response to BMP stimulus Source: Ensembl
  • epithelial cell morphogenesis involved in placental branching Source: MGI
  • establishment or maintenance of cell polarity Source: MGI
  • negative regulation of cell-cell adhesion Source: MGI
  • negative regulation of cell motility Source: MGI
  • negative regulation of neural precursor cell proliferation Source: Ensembl
  • neural tube closure Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-6806942. MET Receptor Activation.

Protein family/group databases

MEROPSiI02.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 2
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 2
Short name:
HAI-2
Gene namesi
Name:Spint2
Synonyms:Hai2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1338031. Spint2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 197ExtracellularSequence analysisAdd BLAST170
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 252CytoplasmicSequence analysisAdd BLAST34

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001688628 – 252Kunitz-type protease inhibitor 2Add BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 88PROSITE-ProRule annotation
Disulfide bondi47 ↔ 71PROSITE-ProRule annotation
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi63 ↔ 84PROSITE-ProRule annotation
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi133 ↔ 183PROSITE-ProRule annotation
Disulfide bondi142 ↔ 166PROSITE-ProRule annotation
Disulfide bondi158 ↔ 179PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9WU03.
PRIDEiQ9WU03.

PTM databases

iPTMnetiQ9WU03.
PhosphoSitePlusiQ9WU03.
SwissPalmiQ9WU03.

Expressioni

Tissue specificityi

Isoform 2 is more predominantly expressed than isoform 1.

Gene expression databases

BgeeiENSMUSG00000074227.
CleanExiMM_SPINT2.
GenevisibleiQ9WU03. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096204.

Structurei

3D structure databases

ProteinModelPortaliQ9WU03.
SMRiQ9WU03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 88BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini133 – 183BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51

Domaini

This inhibitor contains two inhibitory domains.

Sequence similaritiesi

Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000013109.
HOVERGENiHBG079207.
InParanoidiQ9WU03.
OMAiCDNFIYG.
OrthoDBiEOG091G09P2.
PhylomeDBiQ9WU03.
TreeFamiTF326553.

Family and domain databases

Gene3Di4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU03-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQLCELRRG RALLALVASL LLSGAQVASR ELDVHESCGV SKVVGKCRAS
60 70 80 90 100
IPRWWYNITD GSCQPFVYGG CEGNGNNYQS KEECLDKCAG VTENTTDDMA
110 120 130 140 150
RNRNGADSSV LSVPRKQSAE DLSAEIFNYE EYCVPKAVTG PCRAAFPRWY
160 170 180 190 200
YDTEKNSCIS FIYGGCRGNK NSYLSQEACM QHCSGKQMHP FLTPGLKAVI
210 220 230 240 250
LVGLFLMVLI LLLGTSMVCL IRVVRRKQER ALRTVWSTAD DKEQLVKNTC

VL
Length:252
Mass (Da):27,914
Last modified:November 1, 1999 - v1
Checksum:iB2FF4B86924D4F8F
GO
Isoform 2 (identifier: Q9WU03-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-93: Missing.

Show »
Length:195
Mass (Da):21,736
Checksum:iEF49C83AB4E3EDE0
GO
Isoform 3 (identifier: Q9WU03-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-93: Missing.
     114-128: PRKQSAEDLSAEIFN → CFVELSVAALFLFYA
     129-252: Missing.

Show »
Length:71
Mass (Da):7,671
Checksum:iFD28235C68C5692D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00303437 – 93Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_003035114 – 128PRKQS…AEIFN → CFVELSVAALFLFYA in isoform 3. CuratedAdd BLAST15
Alternative sequenceiVSP_003036129 – 252Missing in isoform 3. CuratedAdd BLAST124

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099016 mRNA. Translation: AAD22172.1.
AF099019 mRNA. Translation: AAD22173.1.
AF099020 mRNA. Translation: AAD22174.1.
BC003431 mRNA. Translation: AAH03431.1.
BC026419 mRNA. Translation: AAH26419.1.
CCDSiCCDS39869.1. [Q9WU03-2]
CCDS39870.1. [Q9WU03-1]
PIRiJG0185.
RefSeqiNP_001076017.1. NM_001082548.1. [Q9WU03-2]
NP_035594.1. NM_011464.2. [Q9WU03-1]
UniGeneiMm.295230.

Genome annotation databases

EnsembliENSMUST00000098604; ENSMUSP00000096204; ENSMUSG00000074227. [Q9WU03-1]
ENSMUST00000108236; ENSMUSP00000103871; ENSMUSG00000074227. [Q9WU03-2]
ENSMUST00000207601; ENSMUSP00000146580; ENSMUSG00000074227. [Q9WU03-3]
GeneIDi20733.
KEGGimmu:20733.
UCSCiuc009gbk.1. mouse. [Q9WU03-1]
uc009gbl.1. mouse. [Q9WU03-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099016 mRNA. Translation: AAD22172.1.
AF099019 mRNA. Translation: AAD22173.1.
AF099020 mRNA. Translation: AAD22174.1.
BC003431 mRNA. Translation: AAH03431.1.
BC026419 mRNA. Translation: AAH26419.1.
CCDSiCCDS39869.1. [Q9WU03-2]
CCDS39870.1. [Q9WU03-1]
PIRiJG0185.
RefSeqiNP_001076017.1. NM_001082548.1. [Q9WU03-2]
NP_035594.1. NM_011464.2. [Q9WU03-1]
UniGeneiMm.295230.

3D structure databases

ProteinModelPortaliQ9WU03.
SMRiQ9WU03.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096204.

Protein family/group databases

MEROPSiI02.009.

PTM databases

iPTMnetiQ9WU03.
PhosphoSitePlusiQ9WU03.
SwissPalmiQ9WU03.

Proteomic databases

PaxDbiQ9WU03.
PRIDEiQ9WU03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098604; ENSMUSP00000096204; ENSMUSG00000074227. [Q9WU03-1]
ENSMUST00000108236; ENSMUSP00000103871; ENSMUSG00000074227. [Q9WU03-2]
ENSMUST00000207601; ENSMUSP00000146580; ENSMUSG00000074227. [Q9WU03-3]
GeneIDi20733.
KEGGimmu:20733.
UCSCiuc009gbk.1. mouse. [Q9WU03-1]
uc009gbl.1. mouse. [Q9WU03-2]

Organism-specific databases

CTDi10653.
MGIiMGI:1338031. Spint2.

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000013109.
HOVERGENiHBG079207.
InParanoidiQ9WU03.
OMAiCDNFIYG.
OrthoDBiEOG091G09P2.
PhylomeDBiQ9WU03.
TreeFamiTF326553.

Enzyme and pathway databases

ReactomeiR-MMU-6806942. MET Receptor Activation.

Miscellaneous databases

ChiTaRSiSpint2. mouse.
PROiQ9WU03.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074227.
CleanExiMM_SPINT2.
GenevisibleiQ9WU03. MM.

Family and domain databases

Gene3Di4.10.410.10. 2 hits.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 2 hits.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 2 hits.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPIT2_MOUSE
AccessioniPrimary (citable) accession number: Q9WU03
Secondary accession number(s): Q5D0F2, Q9WU04, Q9WU05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.