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Protein

KH domain-containing, RNA-binding, signal transduction-associated protein 2

Gene

Khdrbs2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (By similarity). Its phosphorylation by FYN inhibits its ability to regulate splice site selection (By similarity). Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer (By similarity). May function as an adapter protein for Src kinases during mitosis. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability.By similarity2 Publications

GO - Molecular functioni

  • poly(A) binding Source: MGI
  • poly(U) RNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • SH2 domain binding Source: MGI
  • SH3/SH2 adaptor activity Source: MGI
  • SH3 domain binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
KH domain-containing, RNA-binding, signal transduction-associated protein 2
Alternative name(s):
Sam68-like mammalian protein 1
Short name:
SLM-1
Short name:
mSLM-1
Gene namesi
Name:Khdrbs2
Synonyms:Slm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2159649. Khdrbs2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349KH domain-containing, RNA-binding, signal transduction-associated protein 2PRO_0000308954Add
BLAST

Post-translational modificationi

Methylated.By similarity
Tyrosine phosphorylated by FYN, PTK6 and SRC. Tyrosine phosphorylated by SRC during mitosis.2 Publications

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9WU01.
MaxQBiQ9WU01.
PaxDbiQ9WU01.
PRIDEiQ9WU01.

PTM databases

iPTMnetiQ9WU01.
PhosphoSiteiQ9WU01.

Expressioni

Tissue specificityi

Expressed in heart, skin, brain, colon, spleen, kidney, cervix and testis.1 Publication

Gene expression databases

BgeeiQ9WU01.
ExpressionAtlasiQ9WU01. baseline and differential.
GenevisibleiQ9WU01. MM.

Interactioni

Subunit structurei

Self-associates to form homooligomers (By similarity). Interacts with RBMX, SAFB, SFRS9 and YTHDC1 (By similarity). Found in a complex with KHDRBS1, KHDRBS2 and KHDRBS3. Interacts with the SH3 domains of FYN and PLCG1. Interacts with the SH2 domains of FYN, GRAP2, PLCG1 and RASA1.By similarity1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • SH2 domain binding Source: MGI
  • SH3/SH2 adaptor activity Source: MGI
  • SH3 domain binding Source: MGI

Protein-protein interaction databases

IntActiQ9WU01. 1 interaction.
MINTiMINT-4131040.
STRINGi10090.ENSMUSP00000027226.

Structurei

3D structure databases

ProteinModelPortaliQ9WU01.
SMRiQ9WU01. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 13571KHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 26252Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the KHDRBS family.Curated
Contains 1 KH domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ9WU01.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ9WU01.
TreeFamiTF314878.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032571. Qua1_dom.
IPR032335. Sam68-YY.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16274. Qua1. 1 hit.
PF16568. Sam68-YY. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEEKYLPEL MAEKDSLDPS FVHASRLLAE EIEKFQGSDG KKEDEEKKYL
60 70 80 90 100
DVISNKNIKL SERVLIPVKQ YPKFNFVGKL LGPRGNSLKR LQEETGAKMS
110 120 130 140 150
ILGKGSMRDK TKEEELRKSG EAKYAHLSDE LHVLIEVFAP PGEAYSRMSH
160 170 180 190 200
ALEEIKKFLV PDYNDEIRQE QLRELSYLNG SEESGRGRGI RGRGIRITPT
210 220 230 240 250
APSRGRGGAV PPPPPPGRGV LTPRGTTVTR GALPVPPIAR GVPTPRARGT
260 270 280 290 300
AAVPGYRAPP PPAHDAYEEY GYDDGYGGEY DDQTYEAYDN SYVTPTQSVP
310 320 330 340
EYYDYGHGVN EDAYDSYAPE EWATTRSSLK APPPRSARGG YREHPYGRY
Length:349
Mass (Da):38,866
Last modified:November 1, 1999 - v1
Checksum:i4B003DA8D94EFED8
GO
Isoform 2 (identifier: Q9WU01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-192: EEELRKSGEA...ESGRGRGIRG → VLSFEVASSH...CRSLSYCYTF
     193-349: Missing.

Show »
Length:193
Mass (Da):21,978
Checksum:iCE5A9FF68D3BD556
GO

Sequence cautioni

The sequence AAH66814.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441D → N in AAH66814 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 19280EEELR…RGIRG → VLSFEVASSHVKQGSSIWSP VYNMWGWRSLFSMRLAFWYH TVAQSEVTIFFHHPPASFSG HSSCVAFCSSLCRSLSYCYT F in isoform 2. 1 PublicationVSP_029065Add
BLAST
Alternative sequencei193 – 349157Missing in isoform 2. 1 PublicationVSP_029066Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098796 mRNA. Translation: AAD20451.1.
BC066814 mRNA. Translation: AAH66814.1. Different initiation.
BC132117 mRNA. Translation: AAI32118.1.
BC132119 mRNA. Translation: AAI32120.1.
CCDSiCCDS14861.1. [Q9WU01-1]
RefSeqiNP_573498.1. NM_133235.2. [Q9WU01-1]
UniGeneiMm.336594.

Genome annotation databases

EnsembliENSMUST00000027226; ENSMUSP00000027226; ENSMUSG00000026058. [Q9WU01-1]
ENSMUST00000188257; ENSMUSP00000140157; ENSMUSG00000026058. [Q9WU01-2]
GeneIDi170771.
KEGGimmu:170771.
UCSCiuc007ano.1. mouse. [Q9WU01-2]
uc007anp.1. mouse. [Q9WU01-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098796 mRNA. Translation: AAD20451.1.
BC066814 mRNA. Translation: AAH66814.1. Different initiation.
BC132117 mRNA. Translation: AAI32118.1.
BC132119 mRNA. Translation: AAI32120.1.
CCDSiCCDS14861.1. [Q9WU01-1]
RefSeqiNP_573498.1. NM_133235.2. [Q9WU01-1]
UniGeneiMm.336594.

3D structure databases

ProteinModelPortaliQ9WU01.
SMRiQ9WU01. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WU01. 1 interaction.
MINTiMINT-4131040.
STRINGi10090.ENSMUSP00000027226.

PTM databases

iPTMnetiQ9WU01.
PhosphoSiteiQ9WU01.

Proteomic databases

EPDiQ9WU01.
MaxQBiQ9WU01.
PaxDbiQ9WU01.
PRIDEiQ9WU01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027226; ENSMUSP00000027226; ENSMUSG00000026058. [Q9WU01-1]
ENSMUST00000188257; ENSMUSP00000140157; ENSMUSG00000026058. [Q9WU01-2]
GeneIDi170771.
KEGGimmu:170771.
UCSCiuc007ano.1. mouse. [Q9WU01-2]
uc007anp.1. mouse. [Q9WU01-1]

Organism-specific databases

CTDi202559.
MGIiMGI:2159649. Khdrbs2.

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ9WU01.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ9WU01.
TreeFamiTF314878.

Miscellaneous databases

ChiTaRSiKhdrbs2. mouse.
NextBioi370395.
PROiQ9WU01.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WU01.
ExpressionAtlasiQ9WU01. baseline and differential.
GenevisibleiQ9WU01. MM.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032571. Qua1_dom.
IPR032335. Sam68-YY.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16274. Qua1. 1 hit.
PF16568. Sam68-YY. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src substrate during mitosis."
    Di Fruscio M., Chen T., Richard S.
    Proc. Natl. Acad. Sci. U.S.A. 96:2710-2715(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, IDENTIFICATION IN A COMPLEX WITH KHDRBS1 AND KHDRBS3, INTERACTION WITH FYN; GRAP2; PLCG1 AND RASA1, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Brain and Neural stem cell.
  3. "The nuclear tyrosine kinase BRK/Sik phosphorylates and inhibits the RNA-binding activities of the Sam68-like mammalian proteins SLM-1 and SLM-2."
    Haegebarth A., Heap D., Bie W., Derry J.J., Richard S., Tyner A.L.
    J. Biol. Chem. 279:54398-54404(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PTK6, FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKHDR2_MOUSE
AccessioniPrimary (citable) accession number: Q9WU01
Secondary accession number(s): Q6NXZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 1, 1999
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.