Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9WU00

- NRF1_MOUSE

UniProt

Q9WU00 - NRF1_MOUSE

Protein

Nuclear respiratory factor 1

Gene

Nrf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi109 – 305197By similarityAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. mitochondrion organization Source: MGI
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear respiratory factor 1
    Short name:
    NRF-1
    Alternative name(s):
    Alpha palindromic-binding protein
    Short name:
    Alpha-pal
    Gene namesi
    Name:Nrf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1332235. Nrf1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503Nuclear respiratory factor 1PRO_0000100209Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei39 – 391Phosphoserine; by CK2By similarity
    Modified residuei44 – 441Phosphoserine; by CK2By similarity
    Modified residuei46 – 461Phosphoserine; by CK2By similarity
    Modified residuei47 – 471Phosphoserine; by CK2By similarity
    Modified residuei52 – 521Phosphoserine; by CK2By similarity

    Post-translational modificationi

    Phosphorylation enhances DNA binding.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9WU00.
    PaxDbiQ9WU00.
    PRIDEiQ9WU00.

    PTM databases

    PhosphoSiteiQ9WU00.

    Expressioni

    Tissue specificityi

    Widely expressed in embryonic, fetal, and adult tissues.

    Gene expression databases

    ArrayExpressiQ9WU00.
    BgeeiQ9WU00.
    CleanExiMM_NRF1.
    GenevestigatoriQ9WU00.

    Interactioni

    Subunit structurei

    Homodimer. Binds DNA as a dimer. Interacts with PPRC1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201845. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WU00.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7878DimerizationBy similarityAdd
    BLAST
    Regioni301 – 476176Required for transcriptional activationBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi88 – 11629Nuclear localization signalBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 6626Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi80 – 867Poly-Ala

    Sequence similaritiesi

    Belongs to the NRF1/Ewg family.Curated

    Phylogenomic databases

    eggNOGiCOG0012.
    GeneTreeiENSGT00390000006835.
    HOGENOMiHOG000273898.
    HOVERGENiHBG003062.
    KOiK11831.
    OMAiDDTDILN.
    OrthoDBiEOG7N37CK.

    Family and domain databases

    InterProiIPR019526. Nrf1_activation-bd.
    IPR019525. Nrf1_NLS/DNA-bd_dimer.
    [Graphical view]
    PfamiPF10492. Nrf1_activ_bdg. 1 hit.
    PF10491. Nrf1_DNA-bind. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q9WU00-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED    50
    TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN 100
    PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA 150
    PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM 200
    TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE 250
    EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATTTHSIAHL 300
    VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA 350
    VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA 400
    TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG 450
    LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT 500
    LEQ 503
    Length:503
    Mass (Da):53,571
    Last modified:July 27, 2011 - v2
    Checksum:i6C3D6B8C83C3B227
    GO
    Isoform Short (identifier: Q9WU00-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         256-321: Missing.

    Show »
    Length:437
    Mass (Da):46,157
    Checksum:iD7B62154D8D015B2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 921K → N in AAD21938. (PubMed:10656923)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei256 – 32166Missing in isoform Short. 1 PublicationVSP_003599Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF098077 mRNA. Translation: AAD21938.1.
    AK014494 mRNA. Translation: BAB29394.1.
    AK029034 mRNA. Translation: BAC26256.1.
    CH466533 Genomic DNA. Translation: EDL13755.1.
    CH466533 Genomic DNA. Translation: EDL13757.1.
    CCDSiCCDS19968.1. [Q9WU00-1]
    RefSeqiNP_001157698.1. NM_001164226.1. [Q9WU00-1]
    NP_035068.3. NM_010938.4. [Q9WU00-1]
    UniGeneiMm.259258.

    Genome annotation databases

    EnsembliENSMUST00000115209; ENSMUSP00000110864; ENSMUSG00000058440. [Q9WU00-1]
    ENSMUST00000115211; ENSMUSP00000110866; ENSMUSG00000058440. [Q9WU00-1]
    ENSMUST00000115212; ENSMUSP00000110867; ENSMUSG00000058440. [Q9WU00-1]
    ENSMUST00000167972; ENSMUSP00000130108; ENSMUSG00000058440. [Q9WU00-1]
    GeneIDi18181.
    KEGGimmu:18181.
    UCSCiuc009bev.1. mouse. [Q9WU00-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF098077 mRNA. Translation: AAD21938.1 .
    AK014494 mRNA. Translation: BAB29394.1 .
    AK029034 mRNA. Translation: BAC26256.1 .
    CH466533 Genomic DNA. Translation: EDL13755.1 .
    CH466533 Genomic DNA. Translation: EDL13757.1 .
    CCDSi CCDS19968.1. [Q9WU00-1 ]
    RefSeqi NP_001157698.1. NM_001164226.1. [Q9WU00-1 ]
    NP_035068.3. NM_010938.4. [Q9WU00-1 ]
    UniGenei Mm.259258.

    3D structure databases

    ProteinModelPortali Q9WU00.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201845. 1 interaction.

    PTM databases

    PhosphoSitei Q9WU00.

    Proteomic databases

    MaxQBi Q9WU00.
    PaxDbi Q9WU00.
    PRIDEi Q9WU00.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000115209 ; ENSMUSP00000110864 ; ENSMUSG00000058440 . [Q9WU00-1 ]
    ENSMUST00000115211 ; ENSMUSP00000110866 ; ENSMUSG00000058440 . [Q9WU00-1 ]
    ENSMUST00000115212 ; ENSMUSP00000110867 ; ENSMUSG00000058440 . [Q9WU00-1 ]
    ENSMUST00000167972 ; ENSMUSP00000130108 ; ENSMUSG00000058440 . [Q9WU00-1 ]
    GeneIDi 18181.
    KEGGi mmu:18181.
    UCSCi uc009bev.1. mouse. [Q9WU00-1 ]

    Organism-specific databases

    CTDi 4899.
    MGIi MGI:1332235. Nrf1.

    Phylogenomic databases

    eggNOGi COG0012.
    GeneTreei ENSGT00390000006835.
    HOGENOMi HOG000273898.
    HOVERGENi HBG003062.
    KOi K11831.
    OMAi DDTDILN.
    OrthoDBi EOG7N37CK.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Miscellaneous databases

    NextBioi 293494.
    PROi Q9WU00.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WU00.
    Bgeei Q9WU00.
    CleanExi MM_NRF1.
    Genevestigatori Q9WU00.

    Family and domain databases

    InterProi IPR019526. Nrf1_activation-bd.
    IPR019525. Nrf1_NLS/DNA-bd_dimer.
    [Graphical view ]
    Pfami PF10492. Nrf1_activ_bdg. 1 hit.
    PF10491. Nrf1_DNA-bind. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Coding sequence, chromosomal localization, and expression pattern of Nrf1: the mouse homolog of Drosophila erect wing."
      Schaefer L., Engman H., Miller J.B.
      Mamm. Genome 11:104-110(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver and Skin.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiNRF1_MOUSE
    AccessioniPrimary (citable) accession number: Q9WU00
    Secondary accession number(s): Q9CXE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3