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Protein

Nuclear respiratory factor 1

Gene

Nrf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi109 – 305197By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_328033. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear respiratory factor 1
Short name:
NRF-1
Alternative name(s):
Alpha palindromic-binding protein
Short name:
Alpha-pal
Gene namesi
Name:Nrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1332235. Nrf1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: AgBase
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Nuclear respiratory factor 1PRO_0000100209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391Phosphoserine; by CK2By similarity
Modified residuei44 – 441Phosphoserine; by CK2By similarity
Modified residuei46 – 461Phosphoserine; by CK2By similarity
Modified residuei47 – 471Phosphoserine; by CK2By similarity
Modified residuei52 – 521Phosphoserine; by CK2By similarity

Post-translational modificationi

Phosphorylation enhances DNA binding.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WU00.
PaxDbiQ9WU00.
PRIDEiQ9WU00.

PTM databases

PhosphoSiteiQ9WU00.

Expressioni

Tissue specificityi

Widely expressed in embryonic, fetal, and adult tissues.

Gene expression databases

BgeeiQ9WU00.
CleanExiMM_NRF1.
ExpressionAtlasiQ9WU00. baseline and differential.
GenevisibleiQ9WU00. MM.

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with PPRC1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201845. 1 interaction.
DIPiDIP-61236N.
STRINGi10090.ENSMUSP00000065568.

Structurei

3D structure databases

ProteinModelPortaliQ9WU00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7878DimerizationBy similarityAdd
BLAST
Regioni301 – 476176Required for transcriptional activationBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi88 – 11629Nuclear localization signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 6626Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi80 – 867Poly-Ala

Sequence similaritiesi

Belongs to the NRF1/Ewg family.Curated

Phylogenomic databases

eggNOGiCOG0012.
GeneTreeiENSGT00390000006835.
HOGENOMiHOG000273898.
HOVERGENiHBG003062.
InParanoidiQ9WU00.
KOiK11831.
OMAiQTEHMTT.
OrthoDBiEOG7N37CK.

Family and domain databases

InterProiIPR019526. Nrf1_activation-bd.
IPR019525. Nrf1_NLS/DNA-bd_dimer.
[Graphical view]
PfamiPF10492. Nrf1_activ_bdg. 1 hit.
PF10491. Nrf1_DNA-bind. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9WU00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
60 70 80 90 100
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
110 120 130 140 150
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
160 170 180 190 200
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
210 220 230 240 250
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
260 270 280 290 300
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATTTHSIAHL
310 320 330 340 350
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
360 370 380 390 400
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
410 420 430 440 450
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
460 470 480 490 500
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT

LEQ
Length:503
Mass (Da):53,571
Last modified:July 27, 2011 - v2
Checksum:i6C3D6B8C83C3B227
GO
Isoform Short (identifier: Q9WU00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-321: Missing.

Show »
Length:437
Mass (Da):46,157
Checksum:iD7B62154D8D015B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921K → N in AAD21938 (PubMed:10656923).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei256 – 32166Missing in isoform Short. 1 PublicationVSP_003599Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098077 mRNA. Translation: AAD21938.1.
AK014494 mRNA. Translation: BAB29394.1.
AK029034 mRNA. Translation: BAC26256.1.
CH466533 Genomic DNA. Translation: EDL13755.1.
CH466533 Genomic DNA. Translation: EDL13757.1.
CCDSiCCDS19968.1. [Q9WU00-1]
RefSeqiNP_001157698.1. NM_001164226.1. [Q9WU00-1]
NP_035068.3. NM_010938.4. [Q9WU00-1]
UniGeneiMm.259258.

Genome annotation databases

EnsembliENSMUST00000115209; ENSMUSP00000110864; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000115211; ENSMUSP00000110866; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000115212; ENSMUSP00000110867; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000167972; ENSMUSP00000130108; ENSMUSG00000058440. [Q9WU00-1]
GeneIDi18181.
KEGGimmu:18181.
UCSCiuc009bev.1. mouse. [Q9WU00-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098077 mRNA. Translation: AAD21938.1.
AK014494 mRNA. Translation: BAB29394.1.
AK029034 mRNA. Translation: BAC26256.1.
CH466533 Genomic DNA. Translation: EDL13755.1.
CH466533 Genomic DNA. Translation: EDL13757.1.
CCDSiCCDS19968.1. [Q9WU00-1]
RefSeqiNP_001157698.1. NM_001164226.1. [Q9WU00-1]
NP_035068.3. NM_010938.4. [Q9WU00-1]
UniGeneiMm.259258.

3D structure databases

ProteinModelPortaliQ9WU00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201845. 1 interaction.
DIPiDIP-61236N.
STRINGi10090.ENSMUSP00000065568.

PTM databases

PhosphoSiteiQ9WU00.

Proteomic databases

MaxQBiQ9WU00.
PaxDbiQ9WU00.
PRIDEiQ9WU00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115209; ENSMUSP00000110864; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000115211; ENSMUSP00000110866; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000115212; ENSMUSP00000110867; ENSMUSG00000058440. [Q9WU00-1]
ENSMUST00000167972; ENSMUSP00000130108; ENSMUSG00000058440. [Q9WU00-1]
GeneIDi18181.
KEGGimmu:18181.
UCSCiuc009bev.1. mouse. [Q9WU00-1]

Organism-specific databases

CTDi4899.
MGIiMGI:1332235. Nrf1.

Phylogenomic databases

eggNOGiCOG0012.
GeneTreeiENSGT00390000006835.
HOGENOMiHOG000273898.
HOVERGENiHBG003062.
InParanoidiQ9WU00.
KOiK11831.
OMAiQTEHMTT.
OrthoDBiEOG7N37CK.

Enzyme and pathway databases

ReactomeiREACT_328033. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

ChiTaRSiNrf1. mouse.
NextBioi293494.
PROiQ9WU00.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WU00.
CleanExiMM_NRF1.
ExpressionAtlasiQ9WU00. baseline and differential.
GenevisibleiQ9WU00. MM.

Family and domain databases

InterProiIPR019526. Nrf1_activation-bd.
IPR019525. Nrf1_NLS/DNA-bd_dimer.
[Graphical view]
PfamiPF10492. Nrf1_activ_bdg. 1 hit.
PF10491. Nrf1_DNA-bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Coding sequence, chromosomal localization, and expression pattern of Nrf1: the mouse homolog of Drosophila erect wing."
    Schaefer L., Engman H., Miller J.B.
    Mamm. Genome 11:104-110(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver and Skin.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiNRF1_MOUSE
AccessioniPrimary (citable) accession number: Q9WU00
Secondary accession number(s): Q9CXE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.