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Protein

Nuclear respiratory factor 1

Gene

Nrf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi109 – 305By similarityAdd BLAST197

GO - Molecular functioni

GO - Biological processi

  • mitochondrion organization Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear respiratory factor 1
Short name:
NRF-1
Alternative name(s):
Alpha palindromic-binding protein
Short name:
Alpha-pal
Gene namesi
Name:Nrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1332235 Nrf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002091 – 503Nuclear respiratory factor 1Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39Phosphoserine; by CK2By similarity1
Modified residuei44Phosphoserine; by CK2By similarity1
Modified residuei46Phosphoserine; by CK2By similarity1
Modified residuei47Phosphoserine; by CK2By similarity1
Modified residuei52Phosphoserine; by CK2By similarity1
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylation enhances DNA binding.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WU00
PaxDbiQ9WU00
PeptideAtlasiQ9WU00
PRIDEiQ9WU00

PTM databases

iPTMnetiQ9WU00
PhosphoSitePlusiQ9WU00

Expressioni

Tissue specificityi

Widely expressed in embryonic, fetal, and adult tissues.

Gene expression databases

BgeeiENSMUSG00000058440
CleanExiMM_NRF1
ExpressionAtlasiQ9WU00 baseline and differential
GenevisibleiQ9WU00 MM

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with PPRC1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
OgtP565582EBI-11291138,EBI-7614183From Rattus norvegicus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201845, 1 interactor
DIPiDIP-61236N
IntActiQ9WU00, 2 interactors
MINTiQ9WU00
STRINGi10090.ENSMUSP00000065568

Structurei

3D structure databases

ProteinModelPortaliQ9WU00
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 78DimerizationBy similarityAdd BLAST78
Regioni301 – 476Required for transcriptional activationBy similarityAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 116Nuclear localization signalBy similarityAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 66Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi80 – 86Poly-Ala7

Sequence similaritiesi

Belongs to the NRF1/Ewg family.Curated

Phylogenomic databases

eggNOGiENOG410IDYK Eukaryota
ENOG410XNU7 LUCA
GeneTreeiENSGT00390000006835
HOGENOMiHOG000273898
HOVERGENiHBG003062
InParanoidiQ9WU00
KOiK11831
OMAiTIHTSQG

Family and domain databases

InterProiView protein in InterPro
IPR019526 Nrf1_activation-bd
IPR019525 Nrf1_NLS/DNA-bd_dimer
PfamiView protein in Pfam
PF10492 Nrf1_activ_bdg, 1 hit
PF10491 Nrf1_DNA-bind, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9WU00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
60 70 80 90 100
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
110 120 130 140 150
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
160 170 180 190 200
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
210 220 230 240 250
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
260 270 280 290 300
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATTTHSIAHL
310 320 330 340 350
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
360 370 380 390 400
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
410 420 430 440 450
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
460 470 480 490 500
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT

LEQ
Length:503
Mass (Da):53,571
Last modified:July 27, 2011 - v2
Checksum:i6C3D6B8C83C3B227
GO
Isoform Short (identifier: Q9WU00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-321: Missing.

Show »
Length:437
Mass (Da):46,157
Checksum:iD7B62154D8D015B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92K → N in AAD21938 (PubMed:10656923).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003599256 – 321Missing in isoform Short. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098077 mRNA Translation: AAD21938.1
AK014494 mRNA Translation: BAB29394.1
AK029034 mRNA Translation: BAC26256.1
CH466533 Genomic DNA Translation: EDL13755.1
CH466533 Genomic DNA Translation: EDL13757.1
CCDSiCCDS19968.1 [Q9WU00-1]
RefSeqiNP_001157698.1, NM_001164226.1 [Q9WU00-1]
NP_035068.3, NM_010938.4 [Q9WU00-1]
XP_017176933.1, XM_017321444.1 [Q9WU00-1]
XP_017176934.1, XM_017321445.1 [Q9WU00-1]
UniGeneiMm.259258

Genome annotation databases

EnsembliENSMUST00000115209; ENSMUSP00000110864; ENSMUSG00000058440 [Q9WU00-1]
ENSMUST00000115211; ENSMUSP00000110866; ENSMUSG00000058440 [Q9WU00-1]
ENSMUST00000115212; ENSMUSP00000110867; ENSMUSG00000058440 [Q9WU00-1]
ENSMUST00000167972; ENSMUSP00000130108; ENSMUSG00000058440 [Q9WU00-1]
GeneIDi18181
KEGGimmu:18181
UCSCiuc009bev.1 mouse [Q9WU00-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNRF1_MOUSE
AccessioniPrimary (citable) accession number: Q9WU00
Secondary accession number(s): Q9CXE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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