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Protein

RING-box protein 2

Gene

Rnf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (By similarity). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Zinc 1By similarity1
Metal bindingi53Zinc 1By similarity1
Metal bindingi61Zinc 3By similarity1
Metal bindingi64Zinc 3By similarity1
Metal bindingi73Zinc 3By similarity1
Metal bindingi80Zinc 2By similarity1
Metal bindingi82Zinc 2By similarity1
Metal bindingi85Zinc 1By similarity1
Metal bindingi87Zinc 3By similarity1
Metal bindingi88Zinc 1By similarity1
Metal bindingi99Zinc 2By similarity1
Metal bindingi102Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 103RING-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic mitochondrial changes Source: MGI
  • cerebral cortex radially oriented cell migration Source: MGI
  • intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • protein neddylation Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • radial glia guided migration of Purkinje cell Source: MGI
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
RING-box protein 2
Short name:
Rbx2
Alternative name(s):
RING finger protein 7
Sensitive to apoptosis gene protein
Gene namesi
Name:Rnf7
Synonyms:Rbx2, Sag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1337096. Rnf7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000560242 – 113RING-box protein 2Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WTZ1.
MaxQBiQ9WTZ1.
PaxDbiQ9WTZ1.
PeptideAtlasiQ9WTZ1.
PRIDEiQ9WTZ1.

PTM databases

PhosphoSitePlusiQ9WTZ1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000051234.
CleanExiMM_RNF7.
MM_SAG.
ExpressionAtlasiQ9WTZ1. baseline and differential.
GenevisibleiQ9WTZ1. MM.

Interactioni

Subunit structurei

Probable part of SCF complexes, which consist of SKP1, CUL1, RNF7/RBX2 and a F-box protein. Interacts (preferentially) with CUL5. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B. Interacts with UBE2F (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202921. 8 interactors.
IntActiQ9WTZ1. 7 interactors.
MINTiMINT-7277486.
STRINGi10090.ENSMUSP00000052856.

Structurei

3D structure databases

ProteinModelPortaliQ9WTZ1.
SMRiQ9WTZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

Sequence similaritiesi

Belongs to the RING-box family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 103RING-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2930. Eukaryota.
COG5194. LUCA.
GeneTreeiENSGT00390000017058.
HOGENOMiHOG000171951.
HOVERGENiHBG001507.
InParanoidiQ9WTZ1.
KOiK10611.
OMAiWIVQRIG.
OrthoDBiEOG091G0TA5.
PhylomeDBiQ9WTZ1.
TreeFamiTF351049.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVEDGEEP CVLSSHSGSA GSKSGGDKMF SLKKWNAVAM WSWDVECDTC
60 70 80 90 100
AICRVQVMDA CLRCQAENKQ EDCVVVWGEC NHSFHNCCMS LWVKQNNRCP
110
LCQQDWVVQR IGK
Length:113
Mass (Da):12,707
Last modified:November 1, 1999 - v1
Checksum:iD6FA02EE6038FDD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092877 mRNA. Translation: AAD25961.1.
AK003248 mRNA. Translation: BAB22666.1.
AK146030 mRNA. Translation: BAE26843.1.
BC011127 mRNA. Translation: AAH11127.1.
CCDSiCCDS40730.1.
RefSeqiNP_001298064.1. NM_001311135.1.
NP_035409.1. NM_011279.3.
UniGeneiMm.28235.

Genome annotation databases

EnsembliENSMUST00000057500; ENSMUSP00000052856; ENSMUSG00000051234.
GeneIDi19823.
KEGGimmu:19823.
UCSCiuc009rcm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092877 mRNA. Translation: AAD25961.1.
AK003248 mRNA. Translation: BAB22666.1.
AK146030 mRNA. Translation: BAE26843.1.
BC011127 mRNA. Translation: AAH11127.1.
CCDSiCCDS40730.1.
RefSeqiNP_001298064.1. NM_001311135.1.
NP_035409.1. NM_011279.3.
UniGeneiMm.28235.

3D structure databases

ProteinModelPortaliQ9WTZ1.
SMRiQ9WTZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202921. 8 interactors.
IntActiQ9WTZ1. 7 interactors.
MINTiMINT-7277486.
STRINGi10090.ENSMUSP00000052856.

PTM databases

PhosphoSitePlusiQ9WTZ1.

Proteomic databases

EPDiQ9WTZ1.
MaxQBiQ9WTZ1.
PaxDbiQ9WTZ1.
PeptideAtlasiQ9WTZ1.
PRIDEiQ9WTZ1.

Protocols and materials databases

DNASUi19823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057500; ENSMUSP00000052856; ENSMUSG00000051234.
GeneIDi19823.
KEGGimmu:19823.
UCSCiuc009rcm.1. mouse.

Organism-specific databases

CTDi9616.
MGIiMGI:1337096. Rnf7.

Phylogenomic databases

eggNOGiKOG2930. Eukaryota.
COG5194. LUCA.
GeneTreeiENSGT00390000017058.
HOGENOMiHOG000171951.
HOVERGENiHBG001507.
InParanoidiQ9WTZ1.
KOiK10611.
OMAiWIVQRIG.
OrthoDBiEOG091G0TA5.
PhylomeDBiQ9WTZ1.
TreeFamiTF351049.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9WTZ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051234.
CleanExiMM_RNF7.
MM_SAG.
ExpressionAtlasiQ9WTZ1. baseline and differential.
GenevisibleiQ9WTZ1. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBX2_MOUSE
AccessioniPrimary (citable) accession number: Q9WTZ1
Secondary accession number(s): Q3UKF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.