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Protein

RING-box protein 2

Gene

Rnf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Zinc 1By similarity
Metal bindingi53 – 531Zinc 1By similarity
Metal bindingi61 – 611Zinc 3By similarity
Metal bindingi64 – 641Zinc 3By similarity
Metal bindingi73 – 731Zinc 3By similarity
Metal bindingi80 – 801Zinc 2By similarity
Metal bindingi82 – 821Zinc 2By similarity
Metal bindingi85 – 851Zinc 1By similarity
Metal bindingi87 – 871Zinc 3By similarity
Metal bindingi88 – 881Zinc 1By similarity
Metal bindingi99 – 991Zinc 2By similarity
Metal bindingi102 – 1021Zinc 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 10343RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic mitochondrial changes Source: MGI
  • cerebral cortex radially oriented cell migration Source: MGI
  • intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • protein neddylation Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway
  • radial glia guided migration of Purkinje cell Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
RING-box protein 2
Short name:
Rbx2
Alternative name(s):
RING finger protein 7
Sensitive to apoptosis gene protein
Gene namesi
Name:Rnf7
Synonyms:Rbx2, Sag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1337096. Rnf7.

Subcellular locationi

GO - Cellular componenti

  • Cul5-RING ubiquitin ligase complex Source: MGI
  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 113112RING-box protein 2PRO_0000056024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WTZ1.
MaxQBiQ9WTZ1.
PaxDbiQ9WTZ1.
PRIDEiQ9WTZ1.

PTM databases

PhosphoSiteiQ9WTZ1.

Expressioni

Gene expression databases

BgeeiQ9WTZ1.
CleanExiMM_RNF7.
MM_SAG.
ExpressionAtlasiQ9WTZ1. baseline and differential.
GenevisibleiQ9WTZ1. MM.

Interactioni

Subunit structurei

Probable part of SCF complexes, which consist of SKP1, CUL1, RNF7/RBX2 and a F-box protein. Interacts (preferentially) with CUL5. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B. Interacts with UBE2F (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202921. 7 interactions.
IntActiQ9WTZ1. 7 interactions.
MINTiMINT-7277486.
STRINGi10090.ENSMUSP00000052856.

Structurei

3D structure databases

ProteinModelPortaliQ9WTZ1.
SMRiQ9WTZ1. Positions 40-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

Sequence similaritiesi

Belongs to the RING-box family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 10343RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2930. Eukaryota.
COG5194. LUCA.
GeneTreeiENSGT00390000017058.
HOGENOMiHOG000171951.
HOVERGENiHBG001507.
InParanoidiQ9WTZ1.
KOiK10611.
OMAiWIVQRIG.
OrthoDBiEOG7CG721.
PhylomeDBiQ9WTZ1.
TreeFamiTF351049.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVEDGEEP CVLSSHSGSA GSKSGGDKMF SLKKWNAVAM WSWDVECDTC
60 70 80 90 100
AICRVQVMDA CLRCQAENKQ EDCVVVWGEC NHSFHNCCMS LWVKQNNRCP
110
LCQQDWVVQR IGK
Length:113
Mass (Da):12,707
Last modified:November 1, 1999 - v1
Checksum:iD6FA02EE6038FDD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092877 mRNA. Translation: AAD25961.1.
AK003248 mRNA. Translation: BAB22666.1.
AK146030 mRNA. Translation: BAE26843.1.
BC011127 mRNA. Translation: AAH11127.1.
CCDSiCCDS40730.1.
RefSeqiNP_001298064.1. NM_001311135.1.
NP_035409.1. NM_011279.3.
UniGeneiMm.28235.

Genome annotation databases

EnsembliENSMUST00000057500; ENSMUSP00000052856; ENSMUSG00000051234.
GeneIDi19823.
KEGGimmu:19823.
UCSCiuc009rcm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092877 mRNA. Translation: AAD25961.1.
AK003248 mRNA. Translation: BAB22666.1.
AK146030 mRNA. Translation: BAE26843.1.
BC011127 mRNA. Translation: AAH11127.1.
CCDSiCCDS40730.1.
RefSeqiNP_001298064.1. NM_001311135.1.
NP_035409.1. NM_011279.3.
UniGeneiMm.28235.

3D structure databases

ProteinModelPortaliQ9WTZ1.
SMRiQ9WTZ1. Positions 40-113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202921. 7 interactions.
IntActiQ9WTZ1. 7 interactions.
MINTiMINT-7277486.
STRINGi10090.ENSMUSP00000052856.

PTM databases

PhosphoSiteiQ9WTZ1.

Proteomic databases

EPDiQ9WTZ1.
MaxQBiQ9WTZ1.
PaxDbiQ9WTZ1.
PRIDEiQ9WTZ1.

Protocols and materials databases

DNASUi19823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057500; ENSMUSP00000052856; ENSMUSG00000051234.
GeneIDi19823.
KEGGimmu:19823.
UCSCiuc009rcm.1. mouse.

Organism-specific databases

CTDi9616.
MGIiMGI:1337096. Rnf7.

Phylogenomic databases

eggNOGiKOG2930. Eukaryota.
COG5194. LUCA.
GeneTreeiENSGT00390000017058.
HOGENOMiHOG000171951.
HOVERGENiHBG001507.
InParanoidiQ9WTZ1.
KOiK10611.
OMAiWIVQRIG.
OrthoDBiEOG7CG721.
PhylomeDBiQ9WTZ1.
TreeFamiTF351049.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9WTZ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTZ1.
CleanExiMM_RNF7.
MM_SAG.
ExpressionAtlasiQ9WTZ1. baseline and differential.
GenevisibleiQ9WTZ1. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SAG, a novel zinc RING finger protein that protects cells from apoptosis induced by redox agents."
    Duan H., Wang Y., Aviram M., Swaroop M., Loo J.A., Bian J., Tian Y., Mueller T., Bisgaier C.L., Sun Y.
    Mol. Cell. Biol. 19:3145-3155(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiRBX2_MOUSE
AccessioniPrimary (citable) accession number: Q9WTZ1
Secondary accession number(s): Q3UKF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.