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Q9WTX8

- MD1L1_MOUSE

UniProt

Q9WTX8 - MD1L1_MOUSE

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Protein
Mitotic spindle assembly checkpoint protein MAD1
Gene
Mad1l1, Mad1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding By similarity.

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. mitotic spindle assembly checkpoint Source: InterPro
  3. regulation of metaphase plate congression Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle assembly checkpoint protein MAD1
Alternative name(s):
Mitotic arrest deficient 1-like protein 1
Short name:
MAD1-like protein 1
Gene namesi
Name:Mad1l1
Synonyms:Mad1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1341857. Mad1l1.

Subcellular locationi

Nucleus By similarity. Chromosomecentromerekinetochore By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore By similarity.

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. condensed chromosome kinetochore Source: UniProtKB-SubCell
  3. kinetochore Source: UniProtKB
  4. microtubule organizing center Source: UniProtKB-SubCell
  5. mitochondrion Source: Ensembl
  6. mitotic spindle Source: UniProtKB
  7. nuclear pore Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Mitotic spindle assembly checkpoint protein MAD1
PRO_0000213801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei16 – 161Phosphoserine By similarity
Modified residuei61 – 611N6-acetyllysine By similarity
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei428 – 4281Phosphoserine By similarity

Post-translational modificationi

Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WTX8.
PaxDbiQ9WTX8.
PRIDEiQ9WTX8.

PTM databases

PhosphoSiteiQ9WTX8.

Expressioni

Inductioni

Increased by TP53 By similarity.

Gene expression databases

BgeeiQ9WTX8.
CleanExiMM_MAD1L1.
GenevestigatoriQ9WTX8.

Interactioni

Subunit structurei

Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex By similarity. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner By similarity.

Protein-protein interaction databases

BioGridi201270. 4 interactions.
IntActiQ9WTX8. 3 interactions.
MINTiMINT-4101621.
STRINGi10090.ENSMUSP00000031534.

Structurei

3D structure databases

ProteinModelPortaliQ9WTX8.
SMRiQ9WTX8. Positions 487-579, 597-715.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni380 – 532153Necessary for interaction with NEK2 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili48 – 631584 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the MAD1 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG275166.
GeneTreeiENSGT00390000001316.
HOGENOMiHOG000004808.
HOVERGENiHBG052441.
InParanoidiQ9WTX8.
KOiK06638.
OMAiGAEEKMQ.
OrthoDBiEOG73FQM6.
PhylomeDBiQ9WTX8.
TreeFamiTF101083.

Family and domain databases

InterProiIPR008672. Mad1.
[Graphical view]
PANTHERiPTHR23168. PTHR23168. 1 hit.
PfamiPF05557. MAD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WTX8-1) [UniParc]FASTAAdd to Basket

Also known as: MAD1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ    50
LEERAEQIRS KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV 100
DRNQELLARI RQLQECEATA EEKMREQLER HRLCKQNLDA VSQQLREQED 150
SLASAREMIS SLKGRVSELQ LSAMDQKVQV KRLESEKQEL KEQLELQQRK 200
WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA VVKSMKSELM 250
RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL 300
VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE 350
KNNSITSSAR GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR 400
NALLTKERDG MRAILGSYDS ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS 450
EMEAQLSQAL EELGVQKQRA DTLEMELKML KAQTSSAESS FSFCKEEVDA 500
LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT KVLHMSLNPI 550
SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV 600
AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL 650
TSRYAEHQTD CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP 700
AFLSALTIEL FSRQTSI 717
Length:717
Mass (Da):83,541
Last modified:November 1, 1999 - v1
Checksum:i4EEB880AE7D80838
GO
Isoform 2 (identifier: Q9WTX8-2) [UniParc]FASTAAdd to Basket

Also known as: MAD1b

The sequence of this isoform differs from the canonical sequence as follows:
     503-717: LKVEELEGER...IELFSRQTSI → VTTTRVEPKCYT

Note: No experimental confirmation available.

Show »
Length:514
Mass (Da):60,150
Checksum:iC5475CDBA7270778
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei503 – 717215LKVEE…RQTSI → VTTTRVEPKCYT in isoform 2.
VSP_011638Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083812 mRNA. Translation: AAD24499.1.
AF083813 mRNA. Translation: AAD24500.1.
BC010702 mRNA. Translation: AAH10702.1.
CCDSiCCDS19817.1. [Q9WTX8-1]
RefSeqiNP_034882.2. NM_010752.3. [Q9WTX8-1]
XP_006504714.1. XM_006504651.1. [Q9WTX8-1]
UniGeneiMm.27250.

Genome annotation databases

EnsembliENSMUST00000031534; ENSMUSP00000031534; ENSMUSG00000029554. [Q9WTX8-1]
ENSMUST00000110829; ENSMUSP00000106453; ENSMUSG00000029554. [Q9WTX8-2]
GeneIDi17120.
KEGGimmu:17120.
UCSCiuc009ahj.1. mouse. [Q9WTX8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083812 mRNA. Translation: AAD24499.1 .
AF083813 mRNA. Translation: AAD24500.1 .
BC010702 mRNA. Translation: AAH10702.1 .
CCDSi CCDS19817.1. [Q9WTX8-1 ]
RefSeqi NP_034882.2. NM_010752.3. [Q9WTX8-1 ]
XP_006504714.1. XM_006504651.1. [Q9WTX8-1 ]
UniGenei Mm.27250.

3D structure databases

ProteinModelPortali Q9WTX8.
SMRi Q9WTX8. Positions 487-579, 597-715.
ModBasei Search...

Protein-protein interaction databases

BioGridi 201270. 4 interactions.
IntActi Q9WTX8. 3 interactions.
MINTi MINT-4101621.
STRINGi 10090.ENSMUSP00000031534.

PTM databases

PhosphoSitei Q9WTX8.

Proteomic databases

MaxQBi Q9WTX8.
PaxDbi Q9WTX8.
PRIDEi Q9WTX8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031534 ; ENSMUSP00000031534 ; ENSMUSG00000029554 . [Q9WTX8-1 ]
ENSMUST00000110829 ; ENSMUSP00000106453 ; ENSMUSG00000029554 . [Q9WTX8-2 ]
GeneIDi 17120.
KEGGi mmu:17120.
UCSCi uc009ahj.1. mouse. [Q9WTX8-1 ]

Organism-specific databases

CTDi 8379.
MGIi MGI:1341857. Mad1l1.

Phylogenomic databases

eggNOGi NOG275166.
GeneTreei ENSGT00390000001316.
HOGENOMi HOG000004808.
HOVERGENi HBG052441.
InParanoidi Q9WTX8.
KOi K06638.
OMAi GAEEKMQ.
OrthoDBi EOG73FQM6.
PhylomeDBi Q9WTX8.
TreeFami TF101083.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

ChiTaRSi MAD1L1. mouse.
NextBioi 291288.
PROi Q9WTX8.
SOURCEi Search...

Gene expression databases

Bgeei Q9WTX8.
CleanExi MM_MAD1L1.
Genevestigatori Q9WTX8.

Family and domain databases

InterProi IPR008672. Mad1.
[Graphical view ]
PANTHERi PTHR23168. PTHR23168. 1 hit.
Pfami PF05557. MAD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5."
    Jin D.-Y., Kozak C.A., Pangilinan F., Spencer F., Green E.D., Jeang K.-T.
    Genomics 55:363-364(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMD1L1_MOUSE
AccessioniPrimary (citable) accession number: Q9WTX8
Secondary accession number(s): Q9WTX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi