Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9WTX8 (MD1L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitotic spindle assembly checkpoint protein MAD1
Alternative name(s):
Mitotic arrest deficient 1-like protein 1
Short name=MAD1-like protein 1
Gene names
Name:Mad1l1
Synonyms:Mad1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length717 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding By similarity.

Subunit structure

Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex By similarity. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromerekinetochore By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Note: From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore By similarity.

Induction

Increased by TP53 By similarity.

Post-translational modification

Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

Sequence similarities

Belongs to the MAD1 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9WTX8-1)

Also known as: MAD1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9WTX8-2)

Also known as: MAD1b;

The sequence of this isoform differs from the canonical sequence as follows:
     503-717: LKVEELEGER...IELFSRQTSI → VTTTRVEPKCYT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 717717Mitotic spindle assembly checkpoint protein MAD1
PRO_0000213801

Regions

Region380 – 532153Necessary for interaction with NEK2 By similarity
Coiled coil48 – 631584 Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue161Phosphoserine By similarity
Modified residue611N6-acetyllysine By similarity
Modified residue2141Phosphoserine By similarity
Modified residue4281Phosphoserine By similarity

Natural variations

Alternative sequence503 – 717215LKVEE…RQTSI → VTTTRVEPKCYT in isoform 2.
VSP_011638

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (MAD1a) [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 4EEB880AE7D80838

FASTA71783,541
        10         20         30         40         50         60 
MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ LEERAEQIRS 

        70         80         90        100        110        120 
KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV DRNQELLARI RQLQECEATA 

       130        140        150        160        170        180 
EEKMREQLER HRLCKQNLDA VSQQLREQED SLASAREMIS SLKGRVSELQ LSAMDQKVQV 

       190        200        210        220        230        240 
KRLESEKQEL KEQLELQQRK WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA 

       250        260        270        280        290        300 
VVKSMKSELM RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL 

       310        320        330        340        350        360 
VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE KNNSITSSAR 

       370        380        390        400        410        420 
GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR NALLTKERDG MRAILGSYDS 

       430        440        450        460        470        480 
ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS EMEAQLSQAL EELGVQKQRA DTLEMELKML 

       490        500        510        520        530        540 
KAQTSSAESS FSFCKEEVDA LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT 

       550        560        570        580        590        600 
KVLHMSLNPI SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV 

       610        620        630        640        650        660 
AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL TSRYAEHQTD 

       670        680        690        700        710 
CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP AFLSALTIEL FSRQTSI 

« Hide

Isoform 2 (MAD1b) [UniParc].

Checksum: C5475CDBA7270778
Show »

FASTA51460,150

References

« Hide 'large scale' references
[1]"Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5."
Jin D.-Y., Kozak C.A., Pangilinan F., Spencer F., Green E.D., Jeang K.-T.
Genomics 55:363-364(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF083812 mRNA. Translation: AAD24499.1.
AF083813 mRNA. Translation: AAD24500.1.
BC010702 mRNA. Translation: AAH10702.1.
CCDSCCDS19817.1. [Q9WTX8-1]
RefSeqNP_034882.2. NM_010752.3. [Q9WTX8-1]
XP_006504714.1. XM_006504651.1. [Q9WTX8-1]
UniGeneMm.27250.

3D structure databases

ProteinModelPortalQ9WTX8.
SMRQ9WTX8. Positions 487-579, 597-715.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201270. 4 interactions.
IntActQ9WTX8. 3 interactions.
MINTMINT-4101621.
STRING10090.ENSMUSP00000031534.

PTM databases

PhosphoSiteQ9WTX8.

Proteomic databases

MaxQBQ9WTX8.
PaxDbQ9WTX8.
PRIDEQ9WTX8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031534; ENSMUSP00000031534; ENSMUSG00000029554. [Q9WTX8-1]
ENSMUST00000110829; ENSMUSP00000106453; ENSMUSG00000029554. [Q9WTX8-2]
GeneID17120.
KEGGmmu:17120.
UCSCuc009ahj.1. mouse. [Q9WTX8-1]

Organism-specific databases

CTD8379.
MGIMGI:1341857. Mad1l1.

Phylogenomic databases

eggNOGNOG275166.
GeneTreeENSGT00390000001316.
HOGENOMHOG000004808.
HOVERGENHBG052441.
InParanoidQ9WTX8.
KOK06638.
OMAGAEEKMQ.
OrthoDBEOG73FQM6.
PhylomeDBQ9WTX8.
TreeFamTF101083.

Gene expression databases

BgeeQ9WTX8.
CleanExMM_MAD1L1.
GenevestigatorQ9WTX8.

Family and domain databases

InterProIPR008672. Mad1.
[Graphical view]
PANTHERPTHR23168. PTHR23168. 1 hit.
PfamPF05557. MAD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAD1L1. mouse.
NextBio291288.
PROQ9WTX8.
SOURCESearch...

Entry information

Entry nameMD1L1_MOUSE
AccessionPrimary (citable) accession number: Q9WTX8
Secondary accession number(s): Q9WTX9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot