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Q9WTX8

- MD1L1_MOUSE

UniProt

Q9WTX8 - MD1L1_MOUSE

Protein

Mitotic spindle assembly checkpoint protein MAD1

Gene

Mad1l1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding By similarity.By similarity

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. mitotic spindle assembly checkpoint Source: InterPro
    3. regulation of metaphase plate congression Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitotic spindle assembly checkpoint protein MAD1
    Alternative name(s):
    Mitotic arrest deficient 1-like protein 1
    Short name:
    MAD1-like protein 1
    Gene namesi
    Name:Mad1l1
    Synonyms:Mad1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1341857. Mad1l1.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromerekinetochore By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. condensed chromosome kinetochore Source: UniProtKB-SubCell
    3. kinetochore Source: UniProtKB
    4. microtubule organizing center Source: UniProtKB-SubCell
    5. mitochondrion Source: Ensembl
    6. mitotic spindle Source: UniProtKB
    7. nuclear pore Source: Ensembl

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 717717Mitotic spindle assembly checkpoint protein MAD1PRO_0000213801Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei16 – 161PhosphoserineBy similarity
    Modified residuei61 – 611N6-acetyllysineBy similarity
    Modified residuei214 – 2141PhosphoserineBy similarity
    Modified residuei428 – 4281PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9WTX8.
    PaxDbiQ9WTX8.
    PRIDEiQ9WTX8.

    PTM databases

    PhosphoSiteiQ9WTX8.

    Expressioni

    Inductioni

    Increased by TP53.By similarity

    Gene expression databases

    BgeeiQ9WTX8.
    CleanExiMM_MAD1L1.
    GenevestigatoriQ9WTX8.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex By similarity. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201270. 4 interactions.
    IntActiQ9WTX8. 3 interactions.
    MINTiMINT-4101621.
    STRINGi10090.ENSMUSP00000031534.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WTX8.
    SMRiQ9WTX8. Positions 487-579, 597-715.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni380 – 532153Necessary for interaction with NEK2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili48 – 631584Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MAD1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG275166.
    GeneTreeiENSGT00390000001316.
    HOGENOMiHOG000004808.
    HOVERGENiHBG052441.
    InParanoidiQ9WTX8.
    KOiK06638.
    OMAiGAEEKMQ.
    OrthoDBiEOG73FQM6.
    PhylomeDBiQ9WTX8.
    TreeFamiTF101083.

    Family and domain databases

    InterProiIPR008672. Mad1.
    [Graphical view]
    PANTHERiPTHR23168. PTHR23168. 1 hit.
    PfamiPF05557. MAD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WTX8-1) [UniParc]FASTAAdd to Basket

    Also known as: MAD1a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ    50
    LEERAEQIRS KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV 100
    DRNQELLARI RQLQECEATA EEKMREQLER HRLCKQNLDA VSQQLREQED 150
    SLASAREMIS SLKGRVSELQ LSAMDQKVQV KRLESEKQEL KEQLELQQRK 200
    WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA VVKSMKSELM 250
    RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL 300
    VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE 350
    KNNSITSSAR GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR 400
    NALLTKERDG MRAILGSYDS ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS 450
    EMEAQLSQAL EELGVQKQRA DTLEMELKML KAQTSSAESS FSFCKEEVDA 500
    LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT KVLHMSLNPI 550
    SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV 600
    AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL 650
    TSRYAEHQTD CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP 700
    AFLSALTIEL FSRQTSI 717
    Length:717
    Mass (Da):83,541
    Last modified:November 1, 1999 - v1
    Checksum:i4EEB880AE7D80838
    GO
    Isoform 2 (identifier: Q9WTX8-2) [UniParc]FASTAAdd to Basket

    Also known as: MAD1b

    The sequence of this isoform differs from the canonical sequence as follows:
         503-717: LKVEELEGER...IELFSRQTSI → VTTTRVEPKCYT

    Note: No experimental confirmation available.

    Show »
    Length:514
    Mass (Da):60,150
    Checksum:iC5475CDBA7270778
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei503 – 717215LKVEE…RQTSI → VTTTRVEPKCYT in isoform 2. 1 PublicationVSP_011638Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083812 mRNA. Translation: AAD24499.1.
    AF083813 mRNA. Translation: AAD24500.1.
    BC010702 mRNA. Translation: AAH10702.1.
    CCDSiCCDS19817.1. [Q9WTX8-1]
    RefSeqiNP_034882.2. NM_010752.3. [Q9WTX8-1]
    XP_006504714.1. XM_006504651.1. [Q9WTX8-1]
    UniGeneiMm.27250.

    Genome annotation databases

    EnsembliENSMUST00000031534; ENSMUSP00000031534; ENSMUSG00000029554. [Q9WTX8-1]
    ENSMUST00000110829; ENSMUSP00000106453; ENSMUSG00000029554. [Q9WTX8-2]
    GeneIDi17120.
    KEGGimmu:17120.
    UCSCiuc009ahj.1. mouse. [Q9WTX8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083812 mRNA. Translation: AAD24499.1 .
    AF083813 mRNA. Translation: AAD24500.1 .
    BC010702 mRNA. Translation: AAH10702.1 .
    CCDSi CCDS19817.1. [Q9WTX8-1 ]
    RefSeqi NP_034882.2. NM_010752.3. [Q9WTX8-1 ]
    XP_006504714.1. XM_006504651.1. [Q9WTX8-1 ]
    UniGenei Mm.27250.

    3D structure databases

    ProteinModelPortali Q9WTX8.
    SMRi Q9WTX8. Positions 487-579, 597-715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201270. 4 interactions.
    IntActi Q9WTX8. 3 interactions.
    MINTi MINT-4101621.
    STRINGi 10090.ENSMUSP00000031534.

    PTM databases

    PhosphoSitei Q9WTX8.

    Proteomic databases

    MaxQBi Q9WTX8.
    PaxDbi Q9WTX8.
    PRIDEi Q9WTX8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031534 ; ENSMUSP00000031534 ; ENSMUSG00000029554 . [Q9WTX8-1 ]
    ENSMUST00000110829 ; ENSMUSP00000106453 ; ENSMUSG00000029554 . [Q9WTX8-2 ]
    GeneIDi 17120.
    KEGGi mmu:17120.
    UCSCi uc009ahj.1. mouse. [Q9WTX8-1 ]

    Organism-specific databases

    CTDi 8379.
    MGIi MGI:1341857. Mad1l1.

    Phylogenomic databases

    eggNOGi NOG275166.
    GeneTreei ENSGT00390000001316.
    HOGENOMi HOG000004808.
    HOVERGENi HBG052441.
    InParanoidi Q9WTX8.
    KOi K06638.
    OMAi GAEEKMQ.
    OrthoDBi EOG73FQM6.
    PhylomeDBi Q9WTX8.
    TreeFami TF101083.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    ChiTaRSi MAD1L1. mouse.
    NextBioi 291288.
    PROi Q9WTX8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WTX8.
    CleanExi MM_MAD1L1.
    Genevestigatori Q9WTX8.

    Family and domain databases

    InterProi IPR008672. Mad1.
    [Graphical view ]
    PANTHERi PTHR23168. PTHR23168. 1 hit.
    Pfami PF05557. MAD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5."
      Jin D.-Y., Kozak C.A., Pangilinan F., Spencer F., Green E.D., Jeang K.-T.
      Genomics 55:363-364(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiMD1L1_MOUSE
    AccessioniPrimary (citable) accession number: Q9WTX8
    Secondary accession number(s): Q9WTX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3