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Protein

S-phase kinase-associated protein 1

Gene

Skp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2 (By similarity).By similarity3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1170546. Prolactin receptor signaling.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-446652. Interleukin-1 family signaling.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5684264. MAP3K8 (TPL2)-dependent MAPK1/3 activation.
R-MMU-69231. Cyclin D associated events in G1.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8939902. Regulation of RUNX2 expression and activity.
R-MMU-8951664. Neddylation.
R-MMU-917937. Iron uptake and transport.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
S-phase kinase-associated protein 1
Alternative name(s):
Cyclin-A/CDK2-associated protein p19
S-phase kinase-associated protein 1A
p19A
p19skp1
Gene namesi
Name:Skp1
Synonyms:Skp1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:103575. Skp1a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872521 – 163S-phase kinase-associated protein 1Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphothreonineCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WTX5.
MaxQBiQ9WTX5.
PaxDbiQ9WTX5.
PRIDEiQ9WTX5.

2D gel databases

REPRODUCTION-2DPAGEiQ9WTX5.

PTM databases

iPTMnetiQ9WTX5.
PhosphoSitePlusiQ9WTX5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036309.
CleanExiMM_SKP1A.
ExpressionAtlasiQ9WTX5. baseline and differential.
GenevisibleiQ9WTX5. MM.

Interactioni

Subunit structurei

Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit. Component of the SCF(FBXW11) complex containing FBXW11. Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1. Component of the SCF(FBXW2) complex containing FBXw2. Component of the SCF(FBXO32) complex containing FBXO32. Component of the probable SCF(FBXO7) complex containing FBXO7. Component of the SCF(FBXO10) complex containing FBXO10. Component of the SCF(FBXO11) complex containing FBXO11. Component of the SCF(FBXO25) complex containing FBXO25. Component of the SCF(FBXO33) complex containing FBXO33. Component of the probable SCF(FBXO4) complex containing FBXO4. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. This complex binds phosphorylated NFKBIA. Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXO2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Component of the SCF(FBXL3) complex composed of CUL1, SKP1, RBX1 and FBXL3. Component of the SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21. Component of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Component of the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7. Interacts with CEP68 (By similarity).By similarity5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203998. 68 interactors.
CORUMiQ9WTX5.
DIPiDIP-38596N.
IntActiQ9WTX5. 52 interactors.
MINTiMINT-4134768.
STRINGi10090.ENSMUSP00000038744.

Structurei

3D structure databases

ProteinModelPortaliQ9WTX5.
SMRiQ9WTX5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 163Interaction with the F-box domain of F-box proteinsBy similarityAdd BLAST60

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

eggNOGiKOG1724. Eukaryota.
COG5201. LUCA.
GeneTreeiENSGT00390000012652.
HOGENOMiHOG000172184.
HOVERGENiHBG057008.
InParanoidiQ9WTX5.
KOiK03094.
OMAiENKWCEE.
OrthoDBiEOG091G0OU2.
PhylomeDBiQ9WTX5.
TreeFamiTF354233.

Family and domain databases

InterProiView protein in InterPro
IPR016897. SKP1.
IPR001232. SKP1-like.
IPR036296. SKP1-like_dim_sf.
IPR011333. SKP1/BTB/POZ_sf.
IPR016072. Skp1_comp_dimer.
IPR016073. Skp1_comp_POZ.
PfamiView protein in Pfam
PF01466. Skp1. 1 hit.
PF03931. Skp1_POZ. 1 hit.
PIRSFiPIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMARTiView protein in SMART
SM00512. Skp1. 1 hit.
SUPFAMiSSF54695. SSF54695. 1 hit.
SSF81382. SSF81382. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WTX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV
60 70 80 90 100
NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL
110 120 130 140 150
FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE
160
AQVRKENQWC EEK
Length:163
Mass (Da):18,672
Last modified:January 23, 2007 - v3
Checksum:iAE184C00BE6556DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41D → N in BAC37220 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083214 mRNA. Translation: AAD16036.1.
AK002983 mRNA. Translation: BAB22496.1.
AK010628 mRNA. Translation: BAB27074.1.
AK012498 mRNA. Translation: BAB28281.1.
AK014245 mRNA. Translation: BAB29222.1.
AK027909 mRNA. Translation: BAC25660.1.
AK078330 mRNA. Translation: BAC37220.1.
AK088339 mRNA. Translation: BAC40292.1.
BC002115 mRNA. Translation: AAH02115.1.
CCDSiCCDS24669.1.
RefSeqiNP_035673.3. NM_011543.4.
XP_006532849.1. XM_006532786.2.
UniGeneiMm.42944.

Genome annotation databases

EnsembliENSMUST00000037324; ENSMUSP00000038744; ENSMUSG00000036309.
ENSMUST00000109072; ENSMUSP00000104700; ENSMUSG00000036309.
GeneIDi21402.
KEGGimmu:21402.
UCSCiuc007ivf.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSKP1_MOUSE
AccessioniPrimary (citable) accession number: Q9WTX5
Secondary accession number(s): Q8C5H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families