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Protein

Glutamate receptor-interacting protein 2

Gene

Grip2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

GO - Biological processi

  • artery smooth muscle contraction Source: Ensembl
  • Notch signaling pathway Source: Ensembl
  • positive regulation of blood pressure Source: Ensembl
  • protein transport Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor-interacting protein 2
Short name:
GRIP-2
Alternative name(s):
AMPA receptor-interacting protein GRIP2
Gene namesi
Name:Grip2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi621668. Grip2.

Subcellular locationi

Isoform 1 :
Isoform 2 :
Isoform 3 :
Isoform 4 :

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • dendrite Source: UniProtKB
  • dendritic shaft Source: UniProtKB
  • dendritic spine Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • membrane Source: RGD
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Glutamate receptor-interacting protein 2PRO_0000083853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphoserineCombined sources
Isoform 4 (identifier: Q9WTW1-4)
Lipidationi11 – 111S-palmitoyl cysteine

Post-translational modificationi

Palmitoylation of isoform 4 mediates membrane location.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9WTW1.
PRIDEiQ9WTW1.

PTM databases

iPTMnetiQ9WTW1.
PhosphoSiteiQ9WTW1.
SwissPalmiQ9WTW1.

Expressioni

Tissue specificityi

Brain specific. Isoform 1 is expressed in the olfactory bulb, cortex and hippocampus and was also expressed at lower levels in thalamus, cerebellum and spinal cord. Isoform 3 is expressed in pyramidal cells of cortex and hippocampus, striatum, thalamus, hypothalamus, stellate cells of cerebellum, and brainstem. Isoform 2 and isoform 4 are expressed in cortex, hippocampus, thalamus, cerebellum, brainstem and spinal cord. In hippocampal neurons isoform 4 is found abundantly in spine structures. Isoform 2 is abundant in the cell body and also found in dendritic shafts.3 Publications

Developmental stagei

Isoform 1 expression was relatively low early in development and increased postnatally, reaching a peak at P14. Isoform 3 is detected at low levels prior to P9, whereupon its expression increases, with the highest levels in the adult.3 Publications

Gene expression databases

BgeeiENSRNOG00000009726.
ExpressionAtlasiQ9WTW1. baseline and differential.
GenevisibleiQ9WTW1. RN.

Interactioni

Subunit structurei

Interacts with the C-terminal tail of PRLHR (By similarity). Interacts with EFNB1, EFNB3, GRIA2, GRIA3, CSGP4 and GRIPAP1. Can form homomultimers and heteromultimers with GRIP1.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cspg4Q8VHY02EBI-936045,EBI-8327479From a different organism.
Gria2P194917EBI-936068,EBI-77718
Grip1P978793EBI-936068,EBI-936113

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9WTW1. 5 interactions.
MINTiMINT-421469.
STRINGi10116.ENSRNOP00000041016.

Structurei

3D structure databases

ProteinModelPortaliQ9WTW1.
SMRiQ9WTW1. Positions 48-345, 450-644, 653-741, 935-1024.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 13684PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 23987PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 33785PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini458 – 54790PDZ 4PROSITE-ProRule annotationAdd
BLAST
Domaini559 – 64385PDZ 5PROSITE-ProRule annotationAdd
BLAST
Domaini658 – 74083PDZ 6PROSITE-ProRule annotationAdd
BLAST
Domaini942 – 102483PDZ 7PROSITE-ProRule annotationAdd
BLAST

Domaini

PDZ 5 mediates the C-terminal binding of GRIA2 and GRIA3. PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1. PDZ 7 mediates interaction with CSPG4.1 Publication

Sequence similaritiesi

Belongs to the GRIP2 family.Curated
Contains 7 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00390000013494.
HOGENOMiHOG000043120.
HOVERGENiHBG051841.
InParanoidiQ9WTW1.
KOiK20251.
OMAiIRLDNCP.
OrthoDBiEOG091G03D1.
PhylomeDBiQ9WTW1.
TreeFamiTF326909.

Family and domain databases

Gene3Di2.30.42.10. 7 hits.
InterProiIPR030029. GRIP2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR10316:SF42. PTHR10316:SF42. 2 hits.
PfamiPF00595. PDZ. 6 hits.
[Graphical view]
SMARTiSM00228. PDZ. 7 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 7 hits.
PROSITEiPS50106. PDZ. 7 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLAVSLKWRL GVVRRRPKDD GPYSKGGKDT AGTDGALVCR RQSIPEEFRG
60 70 80 90 100
ITMVELIKRE GSTLGLTISG GTDKDGKPRV SNLRPGGLAA RSDLLNVGDY
110 120 130 140 150
IRSVNGIRLT RLRHDEIITL LKNVGERVVL EVEYELPPPA PENNPRIISK
160 170 180 190 200
TVDVSLYKEG NSFGFVLRGG AHEDLHKSRP LVLTYVRPGG PADREGSLKV
210 220 230 240 250
GDRLLSIDGI PLHGASHATA IATLQQCSHE ALFQVEYDVA TPDTVANASG
260 270 280 290 300
PLVVEIAKTP GSALGISLTT GSHRNKPAIT IDRIKPASVV DRSGALHAGD
310 320 330 340 350
HILAIDGTST EHCSLVEATK LLASVTEKVR LEILPAPQSR RPLKPPEAVR
360 370 380 390 400
IQRSEQLHHW DPCVPSCHSP RPSHCRAPTW APGGQDQSRS VSSTPFSSPT
410 420 430 440 450
MNPAFPCANA STLPRGPMSP RTTAGRRRQR RKEHRSSLSL ASSTVGPGGQ
460 470 480 490 500
IVHTETTEVV LCGDPLSGFG LQLQGGIFAT ETLSSPPLVR FIEPDSPAER
510 520 530 540 550
CGLLQVGDRV LAINGIATED GTMEEANQLL RDAALARKVV LEIEFDVAES
560 570 580 590 600
VIPSSGTFHV KLPKRRGVEL GITISSASRK RGEPLIISDI KKGSVAHRTG
610 620 630 640 650
TLEPGDKLLA IDNIRLDHCP MEYAVQILRQ CEDLVKLKIR KDEDNSDEQE
660 670 680 690 700
SSGAVSYTVE LKRYGGPLGI TISGTEEPFD PIIISGLTKR GLAERTGAIH
710 720 730 740 750
VGDRILAINS VSLKGRPLSE AIHLLQVAGE TVTLKIKKQL DRPLLPRQSG
760 770 780 790 800
SLSEASDVDE DPPEALKGGL LTTHFSPAVP SVDSAVESWG SSATEGGFGG
810 820 830 840 850
SGSYTPQVAV RSVTPQEWRS SRLKSSPPPL EPRRTSYTPG PTDESFPEEE
860 870 880 890 900
EGDWEPPMSP APGPAREEGF WRVLGEALED LESCGQSELL RELEASIMTG
910 920 930 940 950
TVQSVAVDGR PGSRPWRRSR EVGTSPEDLQ ELLLPTPLEM HRVTLHKDPV
960 970 980 990 1000
RNDFGFSVSD GLLEKGVYVH TVRIDGPAQH GGLQPFDRLL QVNHVRTRDF
1010 1020 1030 1040
DCCLAVPLLA EAGDILELVV SRNPLAQSRR TPGAPGPSSP QMI
Length:1,043
Mass (Da):112,488
Last modified:November 1, 1999 - v1
Checksum:iC463C36ADC459A3C
GO
Isoform 2 (identifier: Q9WTW1-2) [UniParc]FASTAAdd to basket
Also known as: ABP-L, ABP-L/GRIP2

The sequence of this isoform differs from the canonical sequence as follows:
     349-390: VRIQRSEQLHHWDPCVPSCHSPRPSHCRAPTWAPGGQDQSRS → A

Show »
Length:1,002
Mass (Da):107,789
Checksum:i117EB6D94D2091B0
GO
Isoform 3 (identifier: Q9WTW1-3) [UniParc]FASTAAdd to basket
Also known as: ABP, ABP-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     859-874: SPAPGPAREEGFWRVL → RHPVSPLSTPSHLPLF
     875-1043: Missing.

Show »
Length:822
Mass (Da):88,314
Checksum:iEAA388ED9CA1425B
GO
Isoform 4 (identifier: Q9WTW1-4) [UniParc]FASTAAdd to basket
Also known as: pABP-L

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MLAVSLKWRLGVVRRRPK → MRGWRRNLALCLQRLPDE

Show »
Length:1,043
Mass (Da):112,550
Checksum:iCC93D8A6451D2273
GO

Sequence cautioni

The sequence AAD28427 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti450 – 4501Q → H in AAD28427 (PubMed:10414981).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252Missing in isoform 3. 1 PublicationVSP_009760Add
BLAST
Alternative sequencei1 – 1818MLAVS…RRRPK → MRGWRRNLALCLQRLPDE in isoform 4. 1 PublicationVSP_009759Add
BLAST
Alternative sequencei349 – 39042VRIQR…DQSRS → A in isoform 2. 1 PublicationVSP_009761Add
BLAST
Alternative sequencei859 – 87416SPAPG…FWRVL → RHPVSPLSTPSHLPLF in isoform 3. 1 PublicationVSP_009762Add
BLAST
Alternative sequencei875 – 1043169Missing in isoform 3. 1 PublicationVSP_009763Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090113 mRNA. Translation: AAC36313.1.
AF072509 mRNA. Translation: AAD25916.1.
AF205193 mRNA. Translation: AAF19028.1.
AF112182 mRNA. Translation: AAD28427.1. Different initiation.
RefSeqiNP_612544.2. NM_138535.2. [Q9WTW1-1]
XP_006236957.1. XM_006236895.2. [Q9WTW1-2]
UniGeneiRn.15680.

Genome annotation databases

EnsembliENSRNOT00000049657; ENSRNOP00000041016; ENSRNOG00000009726. [Q9WTW1-1]
GeneIDi171571.
KEGGirno:171571.
UCSCiRGD:621668. rat. [Q9WTW1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090113 mRNA. Translation: AAC36313.1.
AF072509 mRNA. Translation: AAD25916.1.
AF205193 mRNA. Translation: AAF19028.1.
AF112182 mRNA. Translation: AAD28427.1. Different initiation.
RefSeqiNP_612544.2. NM_138535.2. [Q9WTW1-1]
XP_006236957.1. XM_006236895.2. [Q9WTW1-2]
UniGeneiRn.15680.

3D structure databases

ProteinModelPortaliQ9WTW1.
SMRiQ9WTW1. Positions 48-345, 450-644, 653-741, 935-1024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WTW1. 5 interactions.
MINTiMINT-421469.
STRINGi10116.ENSRNOP00000041016.

PTM databases

iPTMnetiQ9WTW1.
PhosphoSiteiQ9WTW1.
SwissPalmiQ9WTW1.

Proteomic databases

PaxDbiQ9WTW1.
PRIDEiQ9WTW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000049657; ENSRNOP00000041016; ENSRNOG00000009726. [Q9WTW1-1]
GeneIDi171571.
KEGGirno:171571.
UCSCiRGD:621668. rat. [Q9WTW1-1]

Organism-specific databases

CTDi80852.
RGDi621668. Grip2.

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00390000013494.
HOGENOMiHOG000043120.
HOVERGENiHBG051841.
InParanoidiQ9WTW1.
KOiK20251.
OMAiIRLDNCP.
OrthoDBiEOG091G03D1.
PhylomeDBiQ9WTW1.
TreeFamiTF326909.

Miscellaneous databases

PROiQ9WTW1.

Gene expression databases

BgeeiENSRNOG00000009726.
ExpressionAtlasiQ9WTW1. baseline and differential.
GenevisibleiQ9WTW1. RN.

Family and domain databases

Gene3Di2.30.42.10. 7 hits.
InterProiIPR030029. GRIP2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR10316:SF42. PTHR10316:SF42. 2 hits.
PfamiPF00595. PDZ. 6 hits.
[Graphical view]
SMARTiSM00228. PDZ. 7 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 7 hits.
PROSITEiPS50106. PDZ. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRIP2_RAT
AccessioniPrimary (citable) accession number: Q9WTW1
Secondary accession number(s): O88961, Q9WU36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.