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Protein

Prolactin regulatory element-binding protein

Gene

Preb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.1 Publication
Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.1 Publication

GO - Molecular functioni

GO - Biological processi

  • COPII vesicle coating Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • protein exit from endoplasmic reticulum Source: UniProtKB
  • regulation of transcription, DNA-templated Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene namesi
Name:Preb
Synonyms:Sec12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61929. Preb.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication
  • Nucleus 1 Publication

  • Note: Concentrates at endoplasmic reticulum exit sites.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 388388CytoplasmicSequence analysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence analysisAdd
BLAST
Topological domaini410 – 4178LumenalSequence analysis

GO - Cellular componenti

  • Golgi membrane Source: GOC
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Prolactin regulatory element-binding proteinPRO_0000051156Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Nitrated tyrosineBy similarity

Keywords - PTMi

Nitration

Proteomic databases

PaxDbiQ9WTV0.
PRIDEiQ9WTV0.

PTM databases

iPTMnetiQ9WTV0.
PhosphoSiteiQ9WTV0.

Interactioni

Subunit structurei

Interacts with SAR1B (GDP-bound form) (By similarity). Interacts with CTAGE5; recruits PREB to endoplasmic reticulum exit sites (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4572893.
STRINGi10116.ENSRNOP00000009566.

Structurei

3D structure databases

ProteinModelPortaliQ9WTV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati152 – 19140WD 1Add
BLAST
Repeati194 – 23239WD 2Add
BLAST
Repeati298 – 33740WD 3Add
BLAST

Sequence similaritiesi

Contains 3 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiKOG0771. Eukaryota.
ENOG410XRQK. LUCA.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9WTV0.
KOiK14003.
PhylomeDBiQ9WTV0.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
EQISGCLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQIHQQ
110 120 130 140 150
KGSKAEKSGS KEQGPRQRKG AAPAEKKSGA EVHPEGVELK VKNLEAVQTD
160 170 180 190 200
FSTEPLQKVV CFNHDNTLLA TGGSDGHVRV WKVPSLEKVL EFKAHEGEIG
210 220 230 240 250
DLALGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPTS SNTPYRYQAC
260 270 280 290 300
RFGQVPDQPG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLQTRSCGH
310 320 330 340 350
EVISCLTVSE SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT
360 370 380 390 400
FLPEKGCGPK LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ SAFPGFL
Length:417
Mass (Da):45,357
Last modified:November 23, 2004 - v2
Checksum:i8F059A196CC696FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 543QIS → LIN in AAD28300 (PubMed:10194769).Curated
Sequence conflicti97 – 971I → V in AAD28300 (PubMed:10194769).Curated
Sequence conflicti122 – 1221A → P in AAD28300 (PubMed:10194769).Curated
Sequence conflicti131 – 1311E → Q in AAD28300 (PubMed:10194769).Curated
Sequence conflicti153 – 1531T → N in AAD28300 (PubMed:10194769).Curated
Sequence conflicti174 – 1741S → T in AAD28300 (PubMed:10194769).Curated
Sequence conflicti294 – 2941Q → R in AAD28300 (PubMed:10194769).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061817 mRNA. Translation: AAD28300.1.
BC078936 mRNA. Translation: AAH78936.1.
RefSeqiNP_001164179.1. NM_001170708.1.
UniGeneiRn.41899.

Genome annotation databases

GeneIDi58842.
KEGGirno:58842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061817 mRNA. Translation: AAD28300.1.
BC078936 mRNA. Translation: AAH78936.1.
RefSeqiNP_001164179.1. NM_001170708.1.
UniGeneiRn.41899.

3D structure databases

ProteinModelPortaliQ9WTV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4572893.
STRINGi10116.ENSRNOP00000009566.

PTM databases

iPTMnetiQ9WTV0.
PhosphoSiteiQ9WTV0.

Proteomic databases

PaxDbiQ9WTV0.
PRIDEiQ9WTV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58842.
KEGGirno:58842.

Organism-specific databases

CTDi10113.
RGDi61929. Preb.

Phylogenomic databases

eggNOGiKOG0771. Eukaryota.
ENOG410XRQK. LUCA.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9WTV0.
KOiK14003.
PhylomeDBiQ9WTV0.

Miscellaneous databases

PROiQ9WTV0.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPREB_RAT
AccessioniPrimary (citable) accession number: Q9WTV0
Secondary accession number(s): Q6AYS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.