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Protein

Guanine deaminase

Gene

Gda

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: guanine degradation

This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanine deaminase (Gda)
This subpathway is part of the pathway guanine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Zinc; via tele nitrogenBy similarity1
Metal bindingi84Zinc; via tele nitrogenBy similarity1
Metal bindingi240Zinc; via tele nitrogenBy similarity1
Metal bindingi330ZincBy similarity1
Binding sitei330SubstrateBy similarity1

GO - Molecular functioni

  • guanine deaminase activity Source: RGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • guanine catabolic process Source: UniProtKB-UniPathway
  • positive regulation of microtubule polymerization Source: CACAO

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00603; UER00660.

Protein family/group databases

MEROPSiM38.981.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
Gene namesi
Name:Gda
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621617. Gda.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223001 – 454Guanine deaminaseAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WTT6.
PRIDEiQ9WTT6.

2D gel databases

World-2DPAGEi0004:Q9WTT6.

PTM databases

iPTMnetiQ9WTT6.
PhosphoSitePlusiQ9WTT6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024775.

Structurei

3D structure databases

ProteinModelPortaliQ9WTT6.
SMRiQ9WTT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 87Substrate bindingBy similarity4
Regioni213 – 214Substrate bindingBy similarity2
Regioni240 – 243Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATZ/TRZ family.Curated

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
HOGENOMiHOG000257692.
HOVERGENiHBG005930.
InParanoidiQ9WTT6.
PhylomeDBiQ9WTT6.

Family and domain databases

CDDicd01303. GDEase. 1 hit.
Gene3Di2.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
PANTHERiPTHR11271:SF47. PTHR11271:SF47. 1 hit.
PfamiView protein in Pfam
PF01979. Amidohydro_1. 1 hit.
SFLDiSFLDF00054. guanine_deaminase. 1 hit.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WTT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAARRRELA LIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEESSQ
60 70 80 90 100
QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHAPQYAF AGSNVDLPLL
110 120 130 140 150
DWLNKYTFPT EKRFQSTDVA EEVYTRVVRR TLKNGTTTAC YFGTIHTDSS
160 170 180 190 200
LILAEITDKF GQRAFVGKVC MDLNNTVPEY KETTEESVKE TERFVSEMLQ
210 220 230 240 250
KNYSRVKPIV TPRFSLSCTE TLMSELGNIA KTHDLYIQSH ISENREEIEA
260 270 280 290 300
VKSLYPGYKN YTDVYDKNNL LTNKTVMAHG CYLSEEELNV FSERGASIAH
310 320 330 340 350
CPNSNLSLSS GLLNVLDVLK HKVKIGLGTD VAGGYSYSML DAIRRAVMVS
360 370 380 390 400
NVLLINKVNE KSLTLKEVFR LATLGGSQAL GLDREIGNFE VGKDFDALLI
410 420 430 440 450
NPRASDSPID LFCGDFVGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF

SSSV
Length:454
Mass (Da):51,016
Last modified:November 1, 1999 - v1
Checksum:i518B487A74F99F81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026472 mRNA. Translation: AAD15629.2.
UniGeneiRn.161897.
Rn.24783.

Genome annotation databases

UCSCiRGD:621617. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026472 mRNA. Translation: AAD15629.2.
UniGeneiRn.161897.
Rn.24783.

3D structure databases

ProteinModelPortaliQ9WTT6.
SMRiQ9WTT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024775.

Protein family/group databases

MEROPSiM38.981.

PTM databases

iPTMnetiQ9WTT6.
PhosphoSitePlusiQ9WTT6.

2D gel databases

World-2DPAGEi0004:Q9WTT6.

Proteomic databases

PaxDbiQ9WTT6.
PRIDEiQ9WTT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621617. rat.

Organism-specific databases

RGDi621617. Gda.

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
HOGENOMiHOG000257692.
HOVERGENiHBG005930.
InParanoidiQ9WTT6.
PhylomeDBiQ9WTT6.

Enzyme and pathway databases

UniPathwayiUPA00603; UER00660.

Miscellaneous databases

PROiPR:Q9WTT6.

Family and domain databases

CDDicd01303. GDEase. 1 hit.
Gene3Di2.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
PANTHERiPTHR11271:SF47. PTHR11271:SF47. 1 hit.
PfamiView protein in Pfam
PF01979. Amidohydro_1. 1 hit.
SFLDiSFLDF00054. guanine_deaminase. 1 hit.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGUAD_RAT
AccessioniPrimary (citable) accession number: Q9WTT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: April 12, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.