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Protein

Alpha-(1,6)-fucosyltransferase

Gene

Fut8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.By similarity

Catalytic activityi

GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • cell migration Source: MGI
  • GDP-L-fucose metabolic process Source: UniProtKB
  • integrin-mediated signaling pathway Source: MGI
  • N-glycan fucosylation Source: MGI
  • N-glycan processing Source: MGI
  • protein glycosylation in Golgi Source: InterPro
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • receptor metabolic process Source: MGI
  • regulation of cellular response to oxidative stress Source: MGI
  • regulation of gene expression Source: MGI
  • respiratory gaseous exchange Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-975578. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-(1,6)-fucosyltransferase (EC:2.4.1.68)
Short name:
Alpha1-6FucT
Alternative name(s):
Fucosyltransferase 8
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
GDP-fucose--glycoprotein fucosyltransferase
Glycoprotein 6-alpha-L-fucosyltransferase
Gene namesi
Name:Fut8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1858901. Fut8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 575LumenalSequence analysisAdd BLAST545

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805271 – 575Alpha-(1,6)-fucosyltransferaseAdd BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi204 ↔ 266By similarity
Disulfide bondi212 ↔ 230By similarity
Disulfide bondi218 ↔ 222By similarity
Modified residuei278PhosphoserineCombined sources1
Disulfide bondi465 ↔ 472By similarity

Post-translational modificationi

Tyrosine phosphorylated by PKDCC/VLK.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9WTS2.
MaxQBiQ9WTS2.
PaxDbiQ9WTS2.
PeptideAtlasiQ9WTS2.
PRIDEiQ9WTS2.

PTM databases

iPTMnetiQ9WTS2.
PhosphoSitePlusiQ9WTS2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021065.
CleanExiMM_FUT8.
GenevisibleiQ9WTS2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054530.

Structurei

3D structure databases

ProteinModelPortaliQ9WTS2.
SMRiQ9WTS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 493GT23PROSITE-ProRule annotationAdd BLAST288
Domaini502 – 563SH3Add BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 366Important for donor substrate binding2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi299 – 305SH3-bindingSequence analysis7

Sequence similaritiesi

Belongs to the glycosyltransferase 23 family.PROSITE-ProRule annotation
Contains 1 GT23 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain, SH3-binding, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
GeneTreeiENSGT00530000063737.
HOGENOMiHOG000007175.
HOVERGENiHBG028260.
InParanoidiQ9WTS2.
KOiK00717.
OMAiNARKLVC.
OrthoDBiEOG091G05GD.
TreeFamiTF106108.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL
60 70 80 90 100
ERLKQQNEDL RRMAESLRIP EGPIDQGTAT GRVRVLEEQL VKAKEQIENY
110 120 130 140 150
KKQARNGLGK DHEILRRRIE NGAKELWFFL QSELKKLKHL EGNELQRHAD
160 170 180 190 200
EILLDLGHHE RSIMTDLYYL SQTDGAGDWR EKEAKDLTEL VQRRITYLQN
210 220 230 240 250
PKDCSKARKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT LILESQNWRY
260 270 280 290 300
ATGGWETVFR PVSETCTDRS GLSTGHWSGE VNDKNIQVVE LPIVDSLHPR
310 320 330 340 350
PPYLPLAVPE DLADRLLRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT
360 370 380 390 400
KKLGFKHPVI GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD
410 420 430 440 450
KKRVYLATDD PTLLKEAKTK YSNYEFISDN SISWSAGLHN RYTENSLRGV
460 470 480 490 500
ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ TLHPDASANF HSLDDIYYFG
510 520 530 540 550
GQNAHNQIAV YPHKPRTEEE IPMEPGDIIG VAGNHWDGYS KGINRKLGKT
560 570
GLYPSYKVRE KIETVKYPTY PEAEK
Length:575
Mass (Da):66,557
Last modified:July 27, 2011 - v2
Checksum:i226092A8959B3EB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40S → T in BAA76392 (PubMed:10902914).Curated1
Sequence conflicti388E → Q in BAA76392 (PubMed:10902914).Curated1
Sequence conflicti418K → N in BAA76392 (PubMed:10902914).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025198 mRNA. Translation: BAA76392.1.
AK048520 mRNA. Translation: BAC33357.1.
AK051811 mRNA. Translation: BAC34777.1.
CH466526 Genomic DNA. Translation: EDL36441.1.
CH466526 Genomic DNA. Translation: EDL36443.1.
BC010666 mRNA. Translation: AAH10666.1.
CCDSiCCDS25999.1.
RefSeqiNP_001239543.1. NM_001252614.1.
NP_001239544.1. NM_001252615.1.
NP_001239545.1. NM_001252616.1.
NP_058589.2. NM_016893.5.
XP_011242446.1. XM_011244144.2.
XP_011242447.1. XM_011244145.2.
UniGeneiMm.35628.
Mm.489686.

Genome annotation databases

EnsembliENSMUST00000062804; ENSMUSP00000054530; ENSMUSG00000021065.
ENSMUST00000171770; ENSMUSP00000130845; ENSMUSG00000021065.
ENSMUST00000177595; ENSMUSP00000136327; ENSMUSG00000021065.
GeneIDi53618.
KEGGimmu:53618.
UCSCiuc007nyy.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Fucosyltransferase 8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025198 mRNA. Translation: BAA76392.1.
AK048520 mRNA. Translation: BAC33357.1.
AK051811 mRNA. Translation: BAC34777.1.
CH466526 Genomic DNA. Translation: EDL36441.1.
CH466526 Genomic DNA. Translation: EDL36443.1.
BC010666 mRNA. Translation: AAH10666.1.
CCDSiCCDS25999.1.
RefSeqiNP_001239543.1. NM_001252614.1.
NP_001239544.1. NM_001252615.1.
NP_001239545.1. NM_001252616.1.
NP_058589.2. NM_016893.5.
XP_011242446.1. XM_011244144.2.
XP_011242447.1. XM_011244145.2.
UniGeneiMm.35628.
Mm.489686.

3D structure databases

ProteinModelPortaliQ9WTS2.
SMRiQ9WTS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054530.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

PTM databases

iPTMnetiQ9WTS2.
PhosphoSitePlusiQ9WTS2.

Proteomic databases

EPDiQ9WTS2.
MaxQBiQ9WTS2.
PaxDbiQ9WTS2.
PeptideAtlasiQ9WTS2.
PRIDEiQ9WTS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062804; ENSMUSP00000054530; ENSMUSG00000021065.
ENSMUST00000171770; ENSMUSP00000130845; ENSMUSG00000021065.
ENSMUST00000177595; ENSMUSP00000136327; ENSMUSG00000021065.
GeneIDi53618.
KEGGimmu:53618.
UCSCiuc007nyy.2. mouse.

Organism-specific databases

CTDi2530.
MGIiMGI:1858901. Fut8.

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
GeneTreeiENSGT00530000063737.
HOGENOMiHOG000007175.
HOVERGENiHBG028260.
InParanoidiQ9WTS2.
KOiK00717.
OMAiNARKLVC.
OrthoDBiEOG091G05GD.
TreeFamiTF106108.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiQ9WTS2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021065.
CleanExiMM_FUT8.
GenevisibleiQ9WTS2. MM.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUT8_MOUSE
AccessioniPrimary (citable) accession number: Q9WTS2
Secondary accession number(s): Q543F5, Q921U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.