Q9WTS2 (FUT8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-(1,6)-fucosyltransferase
Short name=Alpha1-6FucT
EC=2.4.1.68

Alternative name(s):
Fucosyltransferase 8
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
GDP-fucose--glycoprotein fucosyltransferase
Glycoprotein 6-alpha-L-fucosyltransferase

Gene names

Name:

Fut8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans By similarity.
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 GT23 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan fucosylation Inferred from electronic annotation. Source: GOC cell migration Inferred from mutant phenotype PubMed 17043354. Source: MGI integrin-mediated signaling pathway Inferred from mutant phenotype PubMed 17043354. Source: MGI protein glycosylation in Golgi Inferred from electronic annotation. Source: InterPro receptor metabolic process Inferred from mutant phenotype PubMed 16236725. Source: MGI respiratory gaseous exchange Inferred from mutant phenotype PubMed 16236725. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype PubMed 16236725. Source: MGI
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-(1->6)-fucosyltransferase activity Inferred from mutant phenotype PubMed 16236725PubMed 17043354. Source: MGI glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Alpha-(1,6)-fucosyltransferase		PRO_0000080527
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 575	545	Lumenal Potential
	Domain	206 – 493	288	GT23
	Domain	502 – 563	62	SH3
	Region	365 – 366	2	Important for donor substrate binding
	Motif	299 – 305	7	SH3-binding Potential
Experimental info
	Sequence conflict	40	1	S → T in BAA76392. Ref.1
	Sequence conflict	388	1	E → Q in BAA76392. Ref.1
	Sequence conflict	418	1	K → N in BAA76392. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9WTS2 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 226092A8959B3EB7	FASTA	575	66,557
	10 20 30 40 50 60 MRAWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIDQGTAT GRVRVLEEQL VKAKEQIENY KKQARNGLGK DHEILRRRIE 130 140 150 160 170 180 NGAKELWFFL QSELKKLKHL EGNELQRHAD EILLDLGHHE RSIMTDLYYL SQTDGAGDWR 190 200 210 220 230 240 EKEAKDLTEL VQRRITYLQN PKDCSKARKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 250 260 270 280 290 300 LILESQNWRY ATGGWETVFR PVSETCTDRS GLSTGHWSGE VNDKNIQVVE LPIVDSLHPR 310 320 330 340 350 360 PPYLPLAVPE DLADRLLRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 370 380 390 400 410 420 GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD KKRVYLATDD PTLLKEAKTK 430 440 450 460 470 480 YSNYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 490 500 510 520 530 540 TLHPDASANF HSLDDIYYFG GQNAHNQIAV YPHKPRTEEE IPMEPGDIIG VAGNHWDGYS 550 560 570 KGINRKLGKT GLYPSYKVRE KIETVKYPTY PEAEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of mouse alpha-1,6-fucosyltransferase and expression of its mRNA in the developing cerebrum." Hayashi H., Yoneda A., Asada M., Ikekita M., Imamura T. DNA Seq. 11:91-96(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye and Head.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Fucosyltransferase 8

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025198 mRNA. Translation: BAA76392.1. AK048520 mRNA. Translation: BAC33357.1. AK051811 mRNA. Translation: BAC34777.1. CH466526 Genomic DNA. Translation: EDL36441.1. CH466526 Genomic DNA. Translation: EDL36443.1. BC010666 mRNA. Translation: AAH10666.1.
IPI	IPI00123777.
RefSeq	NP_001239543.1. NM_001252614.1. NP_001239544.1. NM_001252615.1. NP_001239545.1. NM_001252616.1. NP_058589.2. NM_016893.5.
UniGene	Mm.35628. Mm.489686.
3D structure databases
ProteinModelPortal	Q9WTS2.
SMR	Q9WTS2. Positions 108-572.
ModBase	Search...
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
PTM databases
PhosphoSite	Q9WTS2.
Proteomic databases
PaxDb	Q9WTS2.
PRIDE	Q9WTS2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000062804; ENSMUSP00000054530; ENSMUSG00000021065. ENSMUST00000171770; ENSMUSP00000130845; ENSMUSG00000021065. ENSMUST00000177595; ENSMUSP00000136327; ENSMUSG00000021065.
GeneID	53618.
KEGG	mmu:53618.
Organism-specific databases
CTD	2530.
MGI	MGI:1858901. Fut8.
Phylogenomic databases
eggNOG	NOG251249.
GeneTree	ENSGT00530000063737.
HOGENOM	HOG000007175.
HOVERGEN	HBG028260.
InParanoid	Q543F5.
KO	K00717.
OMA	RKLVCNI.
OrthoDB	EOG4JWVD1.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q9WTS2.
Bgee	Q9WTS2.
CleanEx	MM_FUT8.
Genevestigator	Q9WTS2.
GermOnline	ENSMUSG00000021065. Mus musculus.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR027350. GT23_dom. IPR001452. SH3_domain. [Graphical view]
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS51659. GT23. 1 hit. PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	310339.
SOURCE	Search...

Entry information

Entry name

FUT8_MOUSE

Accession

Primary (citable) accession number: Q9WTS2
Secondary accession number(s): Q543F5, Q921U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program