Reviewed, UniProtKB/Swiss-Prot Q9WTS2 (FUT8_MOUSE)

Names and origin

Protein names

Recommended name:
    Alpha-(1,6)-fucosyltransferase
      Short name=alpha1-6FucT
    EC=2.4.1.68
Alternative name(s):
    Glycoprotein 6-alpha-L-fucosyltransferase
    GDP-fucose--glycoprotein fucosyltransferase
    GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
    Fucosyltransferase 8

Gene names

Name:

Fut8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans By similarity.
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell migration Inferred from mutant phenotype. Source: MGI integrin-mediated signaling pathway Inferred from mutant phenotype. Source: MGI protein amino acid glycosylation in Golgi Inferred from electronic annotation. Source: InterPro receptor metabolic process Inferred from mutant phenotype. Source: MGI respiratory gaseous exchange Inferred from mutant phenotype. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Alpha-(1,6)-fucosyltransferase		PRO_0000080527
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane protein Potential
	Topological domain	31 – 575	545	Lumenal Potential
	Domain	502 – 563	62	SH3
	Region	365 – 366	2	Important for donor substrate binding
	Motif	299 – 305	7	SH3-binding Potential
Experimental info
	Sequence conflict	40	1	T → S in AAH10666. Ref.2
	Sequence conflict	388	1	Q → E in AAH10666. Ref.2
	Sequence conflict	418	1	N → K in AAH10666. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9WTS2-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 7BE2ED3146E0C45F	FASTA	575	66,556
	10 20 30 40 50 60 MRAWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHST RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIDQGTAT GRVRVLEEQL VKAKEQIENY KKQARNGLGK DHEILRRRIE 130 140 150 160 170 180 NGAKELWFFL QSELKKLKHL EGNELQRHAD EILLDLGHHE RSIMTDLYYL SQTDGAGDWR 190 200 210 220 230 240 EKEAKDLTEL VQRRITYLQN PKDCSKARKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 250 260 270 280 290 300 LILESQNWRY ATGGWETVFR PVSETCTDRS GLSTGHWSGE VNDKNIQVVE LPIVDSLHPR 310 320 330 340 350 360 PPYLPLAVPE DLADRLLRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 370 380 390 400 410 420 GVHVRRTDKV GTEAAFHPIE EYMVHVEQHF QLLARRMQVD KKRVYLATDD PTLLKEANTK 430 440 450 460 470 480 YSNYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 490 500 510 520 530 540 TLHPDASANF HSLDDIYYFG GQNAHNQIAV YPHKPRTEEE IPMEPGDIIG VAGNHWDGYS 550 560 570 KGINRKLGKT GLYPSYKVRE KIETVKYPTY PEAEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of mouse alpha-1,6-fucosyltransferase and expression of its mRNA in the developing cerebrum." Hayashi H., Yoneda A., Asada M., Ikekita M., Imamura T. DNA Seq. 11:91-96(2000) [PubMed: 10902914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Fucosyltransferase 8

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB025198 mRNA. Translation: BAA76392.1. BC010666 mRNA. Translation: AAH10666.1.
IPI	IPI00123777.
UniGene	Mm.35628
3D structure databases
SMR	Q9WTS2. Positions 108-572.
ModBase	Search...
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
Proteomic databases
PRIDE	Q9WTS2.
Genome annotation databases
Ensembl	ENSMUSG00000021065. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1858901. Fut8.
Phylogenomic databases
HOGENOM	Q9WTS2.
HOVERGEN	Q9WTS2.
Enzyme and pathway databases
BRENDA	2.4.1.68. 244.
Gene expression databases
ArrayExpress	Q9WTS2.
Bgee	Q9WTS2.
CleanEx	MM_FUT8.
GermOnline	ENSMUSG00000021065. Mus musculus.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

FUT8_MOUSE

Accession

Primary (citable) accession number: Q9WTS2
Secondary accession number(s): Q921U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)