Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9WTR8 (PHLP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PH domain leucine-rich repeat protein phosphatase 1

EC=3.1.3.16
Alternative name(s):
Pleckstrin homology domain-containing family E member 1
Short name=PH domain-containing family E member 1
Suprachiasmatic nucleus circadian oscillatory protein
Gene names
Name:Phlpp1
Synonyms:Phlpp, Plekhe1, Scop
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1696 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-IIand 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor By similarity. May act as a negative regulator of K-Ras signaling in membrane rafts.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Enzyme regulation

Insensitive to okadaic acid By similarity.

Subunit structure

Interacts with the nucleotide free form of K-Ras (KRAS) via its LRR repeats. Interacts with AKT2, AKT3 and PRKCB isoform beta-II By similarity. Ref.2

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein. Nucleus By similarity Ref.2.

Tissue specificity

Mainly present in brain (at protein level). Ref.1

Developmental stage

In the suprachiasmatic nucleus, it increases during subjective night with a peak at midnight under constant dark conditions. Expressed at a constant level in neurons throughout the brain (at protein level). Ref.1

Domain

The PH domain is required for interaction with PRKCB and its dephosphorylation By similarity.

Sequence similarities

Contains 18 LRR (leucine-rich) repeats.

Contains 1 PH domain.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16961696PH domain leucine-rich repeat protein phosphatase 1
PRO_0000057783

Regions

Domain499 – 599101PH
Repeat601 – 62222LRR 1
Repeat624 – 64522LRR 2
Repeat655 – 67622LRR 3
Repeat678 – 69922LRR 4
Repeat701 – 72222LRR 5
Repeat724 – 74623LRR 6
Repeat836 – 85722LRR 7
Repeat858 – 87922LRR 8
Repeat881 – 90222LRR 9
Repeat904 – 92522LRR 10
Repeat926 – 94722LRR 11
Repeat950 – 97122LRR 12
Repeat976 – 99621LRR 13
Repeat1000 – 102122LRR 14
Repeat1024 – 104522LRR 15
Repeat1047 – 106822LRR 16
Repeat1069 – 109022LRR 17
Repeat1092 – 111322LRR 18
Domain1128 – 1383256PP2C-like
Motif1694 – 16963PDZ-binding
Compositional bias4 – 96Poly-Ala
Compositional bias21 – 288Poly-Ala
Compositional bias116 – 12611Poly-Ser
Compositional bias339 – 3435Poly-Ser
Compositional bias405 – 4106Poly-Ala
Compositional bias1431 – 14344Poly-Ser
Compositional bias1645 – 166117Poly-Gln
Compositional bias1662 – 16698Poly-Pro

Sites

Metal binding11731Manganese 1 By similarity
Metal binding11731Manganese 2 By similarity
Metal binding11741Manganese 1; via carbonyl oxygen By similarity
Metal binding13371Manganese 2 By similarity
Metal binding13761Manganese 2 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9WTR8 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B4C3CC777B8BB6A9

FASTA1,696183,364
        10         20         30         40         50         60 
MEPAAAAPAQ RLADPTGEDR APAAAAAAEG GRSPDSVLSA AAPSGGNGGA AREEAPCEAP 

        70         80         90        100        110        120 
PGPLPGRAGG TGRRRRRGVP QPAAGGAAPV TAAGGGANSL LLRRGRLKRN LSAAASSSSS 

       130        140        150        160        170        180 
PSSASSAAGG LPASCSASAS LCTRSLDRKT LLQKHRQLLQ LQPSDRDWVR HQLQRGCVHV 

       190        200        210        220        230        240 
FDRHMASSYL RPVLCTLDTT AAEVAARLLQ LGHKGGGVVK VLGHGPPPAA APAASDQTPA 

       250        260        270        280        290        300 
TELGRDVEPP PSSSTVGAVR GPARAPPADL PLPGGAWTRC APRVNPAPSD SSPGELFAGG 

       310        320        330        340        350        360 
PCSPSRAPRP ASDTESFSLS PSAESVSDRL DPYSSGGGSS SSSEELEADP ATVLTGPSGP 

       370        380        390        400        410        420 
PHHPVRSSQP RPPSPKTSAL LQPKAPTGVD GTGLVVGEGP GDDKAVAAAA PGVPLWTPGR 

       430        440        450        460        470        480 
IRETVQKTSS PPSLYVQLHG ETTRRLEADE KPLQIQNDYL FQLGFGELWR VQEEGMDSEI 

       490        500        510        520        530        540 
GCLIRFYAGK PHSTGSSERI QLSGMYNVRK GKMQLPVNRW TRRQVILCGT CLIVSSVKDS 

       550        560        570        580        590        600 
SSGKMHVLPL IGGKVEEVKK HQHCLAFSSS GPQSQTYYIC FDTFTEYLRW LRQVSKVASQ 

       610        620        630        640        650        660 
RISSVDLSCC SLEHLPANLF YSQDLTHLNL KQNFLRQNPS LPAARGLGEL QRFTKLKSLN 

       670        680        690        700        710        720 
LSNNHLGAFP SAVCSIPTLA ELNVSCNALQ EVPAAVGAMQ NLQTFLLDGN FLQSLPAELE 

       730        740        750        760        770        780 
NMHQLSYLGL SFNEFTDIPE VLEKLTAVDK LCMAGNCMET LRLQALRRMP HIKHVDLRLN 

       790        800        810        820        830        840 
ILRKLITDEV DFLQHVTQLD LRDNKLGDLD AMIFNNIEVL HCERNQLVTL NICGYFLKAL 

       850        860        870        880        890        900 
YASSNELVQL DVYPVPNYLS YMDVSRNCLE SVPEWVCESR KLEVLDIGHN QICELPARLF 

       910        920        930        940        950        960 
CNSSLRKLLA GHNRLARLPE RLERTSVEVL DVQHNQIIEL PPNLLMKADS LRFLNASANK 

       970        980        990       1000       1010       1020 
LETLPPATLS EETSSILQEL YLTNNSLTDK CVPLLTGHPR LKILHMAYNR LQSFPASKMA 

      1030       1040       1050       1060       1070       1080 
KLEELEEIDI SGNKLKAIPT TIMNCRRMHT VIAHSNCIEV FPEVMQLPEV KCVDLSCNEL 

      1090       1100       1110       1120       1130       1140 
SEITLPENLP PKLQELDLTG NPRLALDHKS LELLNNIRCF KIDQPSAGDA SGAPAVWSHG 

      1150       1160       1170       1180       1190       1200 
YTEASGVKNK LCVAALSVNN FRDNREALYG VFDGDRNVEV PYLLQCTMSD ILAEELQKTK 

      1210       1220       1230       1240       1250       1260 
NEEEYMVNTF IVMQRKLGTA GQKLGGAAVL CHIRHDPVDL GGSFTLTSAN VGKCQTVLCR 

      1270       1280       1290       1300       1310       1320 
NGKPLSLSRS YTMSCEEERK RIKQHKAIIT EDGKVNGVTE STRILGYTFL HPSVVPRPHV 

      1330       1340       1350       1360       1370       1380 
QSVLLTPQDE FFILGSKGLW DSLSIEEAVE AVRNVPDALA AAKKLCTLAQ SYGCHDSISA 

      1390       1400       1410       1420       1430       1440 
VVVQLSVTED SFCCCELSVG GSMPPPSPGI FPPSVSMVIK DRPSDGLGVP SSSSGMASEI 

      1450       1460       1470       1480       1490       1500 
SSELSTSEMS SEVGSTASDE PPSGALSESS PAYPSEQRCM LHPVCLSNSF QRQLSSATFS 

      1510       1520       1530       1540       1550       1560 
SAFSDNGLDS DDEEPIEGVF SNGSRVEVEV DIHCSRAKEK ERQQHLLQVP AEASDEGIVI 

      1570       1580       1590       1600       1610       1620 
SANEDESGLS KKTDISAVGT IGRRRANGSV PPQERSHNVI EVATDAPLRK PGGYFAAPAQ 

      1630       1640       1650       1660       1670       1680 
PDPDDQFIIP PELEEEVKEI MKHHQEQQQQ QQQQQQQQQQ QPPPPPQPPQ AQAQAQAQAQ 

      1690 
RPFQMDHLPD CYDTPL 

« Hide

References

[1]"SCOP, a novel gene product expressed in a circadian manner in rat suprachiasmatic nucleus."
Shimizu K., Okada M., Takano A., Nagai K.
FEBS Lett. 458:363-369(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Brain.
[2]"Suprachiasmatic nucleus circadian oscillatory protein, a novel binding partner of K-Ras in the membrane rafts, negatively regulates MAPK pathway."
Shimizu K., Okada M., Nagai K., Fukada Y.
J. Biol. Chem. 278:14920-14925(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH KRAS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023624 mRNA. Translation: BAA77767.1.
RefSeqNP_067689.1. NM_021657.1.
UniGeneRn.163214.

3D structure databases

ProteinModelPortalQ9WTR8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248740. 1 interaction.
IntActQ9WTR8. 2 interactions.
STRING10116.ENSRNOP00000003840.

Proteomic databases

PaxDbQ9WTR8.
PRIDEQ9WTR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID59265.
KEGGrno:59265.
UCSCRGD:621308. rat.

Organism-specific databases

CTD23239.
RGD621308. Phlpp1.

Phylogenomic databases

eggNOGCOG4886.
HOGENOMHOG000115529.
InParanoidQ9WTR8.
KOK16340.
PhylomeDBQ9WTR8.

Gene expression databases

GenevestigatorQ9WTR8.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001611. Leu-rich_rpt.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001932. PP2C-like_dom.
[Graphical view]
PfamPF00560. LRR_1. 2 hits.
PF13855. LRR_8. 2 hits.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS51450. LRR. 17 hits.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611783.
PROQ9WTR8.

Entry information

Entry namePHLP1_RAT
AccessionPrimary (citable) accession number: Q9WTR8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: November 1, 1999
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families