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Protein

Mitogen-activated protein kinase kinase kinase 6

Gene

Map3k6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on Thr-807. Catalytically active only when complexed with MAP3K5, with MAP3K5 supporting the stability and the active configuration of MAP3K6 and MAP3K6 activating MAP3K5 by direct phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei678ATPPROSITE-ProRule annotation1 Publication1
Active sitei772Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi655 – 663ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.25. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 6 (EC:2.7.11.25)
Alternative name(s):
Apoptosis signal-regulating kinase 2
Gene namesi
Name:Map3k6Imported
Synonyms:Ask2, Mapkkk6By similarity, Mekk6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1855691. Map3k6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi678K → M: Loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002916481 – 1291Mitogen-activated protein kinase kinase kinase 6Add BLAST1291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei807Phosphothreonine1 Publication1
Modified residuei965PhosphoserineBy similarity1
Modified residuei985PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WTR2.
PaxDbiQ9WTR2.
PRIDEiQ9WTR2.

PTM databases

iPTMnetiQ9WTR2.
PhosphoSitePlusiQ9WTR2.

Expressioni

Gene expression databases

CleanExiMM_MAP3K6.

Interactioni

Subunit structurei

Binds both upstream activators and downstream substrates in multimolecular complexes.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAP3K5Q996833EBI-1254790,EBI-476263From a different organism.

Protein-protein interaction databases

IntActiQ9WTR2. 2 interactors.
STRINGi10090.ENSMUSP00000030677.

Structurei

3D structure databases

ProteinModelPortaliQ9WTR2.
SMRiQ9WTR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini649 – 907Protein kinasePROSITE-ProRule annotationAdd BLAST259

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1006 – 1069Sequence analysisAdd BLAST64
Coiled coili1164 – 1195Sequence analysisAdd BLAST32

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4279. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000293286.
HOVERGENiHBG006305.
InParanoidiQ9WTR2.
PhylomeDBiQ9WTR2.
TreeFamiTF105115.

Family and domain databases

InterProiIPR025136. DUF4071.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR013761. SAM/pointed.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13281. DUF4071. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPCPRAGV LERAGSCWQD PLAEALSRGR SSPAVTGRGC ARSRPLSVVY
60 70 80 90 100
VLTREPGPGV EPGSGTEAEP LPLRCLREAC AQLQGTRPPP QLRSLPFATL
110 120 130 140 150
ALGDTAALDS FYNADVVVLE VSSSLAQPSL FYHLGVRESF SMTNNVLLCS
160 170 180 190 200
QAELPDLQAL REDVFQKNSD CVGSYTLIPY VVTATGRVLC GDAGLLRGIA
210 220 230 240 250
DGLVQAGAGT EALLTPLVGR LVRLLEATPT DSCGYFRETI RQDIRQARER
260 270 280 290 300
FSGQQLRQEL ARLQRRLDSV ELLSPDIVMN LLLSYRDVQD YSAIIELVET
310 320 330 340 350
LQALPTCDVA EQHNVCFHYT FALNRRNRPG DREKALAVLL PLVKYEGPVA
360 370 380 390 400
PDLYCMCGRI YKDMFFTSGF QNAGHLEQAY HWYRKAFDVE PSLHSGINAA
410 420 430 440 450
VLLIAAGQHF EDSEELRLIG MKLACLLARK GCVEKMQYYW DVGFYLGAQI
460 470 480 490 500
LANDPIQVVL AAEQLYKLNA PIWYLVSVME TFLLYQHFRP TPEPSGGPPL
510 520 530 540 550
RAHFWLHFLL QSCQPFKMAP PQEDQCLVLV LEINKVLLPA RLEIQGTDPM
560 570 580 590 600
SAVTLSLLEP ETQEDPSSWT FPVTSICGIS TSKLDQRCCF LYALPPAQDV
610 620 630 640 650
QLCFPSVERC QRFCGLIQVL VMNPDSSAPT EEAEGAREVL EFDYEYSETG
660 670 680 690 700
ERLVLGRGTY GVVYAGRDRH TRVRIAIKEI PERDSRFSQP LHEEIALHKR
710 720 730 740 750
LRHKNIVRYL GSASQGGYLK IFMEEVPGGS LSSLLRSVWG PLKDNESTIS
760 770 780 790 800
FYTRQILQGL SYLHENRIVH RDIKGDNVLI NTFSGLLKIS DFGTSKRLAG
810 820 830 840 850
ITPCTETFTG TLQYMAPEII DQGPRGYGKA ADIWSLGCTV IEMATGRPPF
860 870 880 890 900
HELGSPQAAM FQVGMYKVHP PVPGSLSAEA QAFLLRTFEP DPRLRASAQE
910 920 930 940 950
LLGDPFLQPG KRSRSPGSPR HTPRPSGAPS GPSSPSADSA TQSQTFPRPQ
960 970 980 990 1000
APSQHPPSPP KRCLSYGDTS QLRVPEEPAA EEPASPEESS GLSLLHQESK
1010 1020 1030 1040 1050
RRAMLAAVLE QEVPTLAENL LEQEQDSRLS KIHVELLLRC LGAQIHTPNR
1060 1070 1080 1090 1100
RQLAQELRTL QAQLRAQSLG PALLKGPLFA FPDAVKQILR RRQIRPHWMF
1110 1120 1130 1140 1150
VLDSLLSRAV RAALAVLDAE SEKKAVLPRS EESSKEESQQ KPQESQALQS
1160 1170 1180 1190 1200
QLPPEQGPPS LMVELGLLRA ETDRLRDLLA EKERECQALV QQALHRVHAE
1210 1220 1230 1240 1250
TRKYAPASET PATLPKDQNL VRWLQELSVD PATIQTLLSH SFTLQTLLTC
1260 1270 1280 1290
ATQDDLVYTR IRGGMVCRIW RAILAQRAGA TSVTPVPRDA E
Length:1,291
Mass (Da):143,055
Last modified:June 26, 2007 - v4
Checksum:iFC61A90935A7465D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7R → G in CAM21067 (PubMed:19468303).Curated1
Sequence conflicti7R → G in CAM18232 (PubMed:19468303).Curated1
Sequence conflicti58P → L in CAM21067 (PubMed:19468303).Curated1
Sequence conflicti58P → L in CAM18232 (PubMed:19468303).Curated1
Sequence conflicti550 – 552MSA → VST in CAM21067 (PubMed:19468303).Curated3
Sequence conflicti550 – 552MSA → VST in CAM18232 (PubMed:19468303).Curated3
Sequence conflicti559E → D in CAM21067 (PubMed:19468303).Curated1
Sequence conflicti559E → D in CAM18232 (PubMed:19468303).Curated1
Sequence conflicti1114L → M in CAM21067 (PubMed:19468303).Curated1
Sequence conflicti1114L → M in CAM18232 (PubMed:19468303).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021861 mRNA. Translation: BAA78532.4.
AL671882, AL837525 Genomic DNA. Translation: CAM21067.1.
AL837525, AL671882 Genomic DNA. Translation: CAM18232.1.
BC120565 mRNA. Translation: AAI20566.1.
BC125577 mRNA. Translation: AAI25578.1.
CCDSiCCDS18744.1.
UniGeneiMm.36640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021861 mRNA. Translation: BAA78532.4.
AL671882, AL837525 Genomic DNA. Translation: CAM21067.1.
AL837525, AL671882 Genomic DNA. Translation: CAM18232.1.
BC120565 mRNA. Translation: AAI20566.1.
BC125577 mRNA. Translation: AAI25578.1.
CCDSiCCDS18744.1.
UniGeneiMm.36640.

3D structure databases

ProteinModelPortaliQ9WTR2.
SMRiQ9WTR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WTR2. 2 interactors.
STRINGi10090.ENSMUSP00000030677.

PTM databases

iPTMnetiQ9WTR2.
PhosphoSitePlusiQ9WTR2.

Proteomic databases

MaxQBiQ9WTR2.
PaxDbiQ9WTR2.
PRIDEiQ9WTR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1855691. Map3k6.

Phylogenomic databases

eggNOGiKOG4279. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000293286.
HOVERGENiHBG006305.
InParanoidiQ9WTR2.
PhylomeDBiQ9WTR2.
TreeFamiTF105115.

Enzyme and pathway databases

BRENDAi2.7.11.25. 3474.

Miscellaneous databases

PROiQ9WTR2.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MAP3K6.

Family and domain databases

InterProiIPR025136. DUF4071.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR013761. SAM/pointed.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13281. DUF4071. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM3K6_MOUSE
AccessioniPrimary (citable) accession number: Q9WTR2
Secondary accession number(s): A2AE93, Q0VBN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.