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Q9WTR0

- MMP16_MOUSE

UniProt

Q9WTR0 - MMP16_MOUSE

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Protein
Matrix metalloproteinase-16
Gene
Mmp16
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells By similarity.

Cofactori

Binds 1 zinc ion per subunit By similarity.
Calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Zinc 1; in inhibited form By similarity
Metal bindingi183 – 1831Calcium 1; via carbonyl oxygen By similarity
Metal bindingi193 – 1931Zinc 1 By similarity
Metal bindingi195 – 1951Zinc 1 By similarity
Metal bindingi200 – 2001Calcium 2 By similarity
Metal bindingi201 – 2011Calcium 2; via carbonyl oxygen By similarity
Metal bindingi203 – 2031Calcium 2; via carbonyl oxygen By similarity
Metal bindingi205 – 2051Calcium 2; via carbonyl oxygen By similarity
Metal bindingi215 – 2151Calcium 1; via carbonyl oxygen By similarity
Metal bindingi217 – 2171Calcium 1; via carbonyl oxygen By similarity
Metal bindingi219 – 2191Calcium 1 By similarity
Metal bindingi223 – 2231Calcium 2 By similarity
Metal bindingi226 – 2261Calcium 2 By similarity
Metal bindingi246 – 2461Zinc 2; catalytic By similarity
Active sitei247 – 2471 By similarity
Metal bindingi250 – 2501Zinc 2; catalytic By similarity
Metal bindingi256 – 2561Zinc 2; catalytic By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. bone development Source: MGI
  2. chondrocyte proliferation Source: MGI
  3. collagen catabolic process Source: MGI
  4. craniofacial suture morphogenesis Source: MGI
  5. embryonic cranial skeleton morphogenesis Source: MGI
  6. endochondral ossification Source: MGI
  7. ossification Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiM10.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-16 (EC:3.4.24.-)
Short name:
MMP-16
Alternative name(s):
Membrane-type matrix metalloproteinase 3
Short name:
MT-MMP 3
Short name:
MTMMP3
Membrane-type-3 matrix metalloproteinase
Short name:
MT3-MMP
Short name:
MT3MMP
Gene namesi
Name:Mmp16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1276107. Mmp16.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein; Extracellular side Reviewed prediction
Note: Localized at the cell surface of melanoma cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini120 – 564445Extracellular Reviewed prediction
Add
BLAST
Transmembranei565 – 58521Helical; Reviewed prediction
Add
BLAST
Topological domaini586 – 60722Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. extracellular matrix Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Propeptidei32 – 11988 By similarity
PRO_0000028814Add
BLAST
Chaini120 – 607488Matrix metalloproteinase-16
PRO_0000028815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi343 ↔ 532 By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ9WTR0.

PTM databases

PhosphoSiteiQ9WTR0.

Expressioni

Gene expression databases

ArrayExpressiQ9WTR0.
BgeeiQ9WTR0.
CleanExiMM_MMP16.
GenevestigatoriQ9WTR0.

Interactioni

Subunit structurei

Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029881.

Structurei

3D structure databases

ProteinModelPortaliQ9WTR0.
SMRiQ9WTR0. Positions 85-561.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati340 – 38849Hemopexin 1
Add
BLAST
Repeati389 – 43446Hemopexin 2
Add
BLAST
Repeati436 – 48449Hemopexin 3
Add
BLAST
Repeati485 – 53248Hemopexin 4
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi99 – 1068Cysteine switch By similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.
Contains 4 hemopexin repeats.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295915.
GeneTreeiENSGT00750000117332.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ6PEQ6.
KOiK07996.
OMAiVPPHRSV.
OrthoDBiEOG7XPZ57.
TreeFamiTF352396.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028697. MMP16.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF26. PTHR10201:SF26. 1 hit.
PfamiPF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTR0-1 [UniParc]FASTAAdd to Basket

« Hide

MILLAFSSGR RLDFVHRSGV FFLQTLLWIL CATVCGTEQY FNVEVWLQKY    50
GYLPPTDPRM SVLRSAETMQ SALAAMQQFY GINMTGKVDR NTIDWMKKPR 100
CGVPDQTRGS SKFNIRRKRY ALTGQKWQHK HITYSIKNVT PKVGDPETRR 150
AIRRAFDVWQ NVTPLTFEEV PYSELENGKR DVDITIIFAS GFHGDSSPFD 200
GEGGFLAHAY FPGPGIGGDT HFDSDEPWTL GNPNHDGNDL FLVAVHELGH 250
ALGLEHSNDP TAIMAPFYQY METDNFKLPN DDLQGIQKIY GPPDKIPPPT 300
RPLPTVPPHR SVPPADPRRH DRPKPPRPPT GRPSYPGAKP NICDGNFNTL 350
AILRREMFVF KDQWFWRVRN NRVMDGYPMQ ITYFWRGLPP SIDAVYENSD 400
GNFVFFKGNK YWVFKDTTLQ PGYPHDLITL GNGIPPHGID SAIWWEDVGK 450
TYFFKGDRYW RYSEEMKTMD PGYPKPITIW KGIPESPQGA FVHKENGFTY 500
FYKGKEYWKF NNQILKVEPG YPRSILKDFM GCDGPTDRDK EGLSPPDDVD 550
IVIKLDNTAS TVKAIAIVIP CILALCLLVL VYTVFQFKRK GTPRHILYCK 600
RSMQEWV 607
Length:607
Mass (Da):69,571
Last modified:July 27, 2011 - v3
Checksum:iC4CB54F75B26A296
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171R → H in BAA78420. 1 Publication
Sequence conflicti19 – 191G → V in AAG17704. 1 Publication
Sequence conflicti196 – 1961S → R in AAG17704. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021228 mRNA. Translation: BAA78420.1.
AF282844 mRNA. Translation: AAG17704.1.
AL683877, AL805957 Genomic DNA. Translation: CAM13981.1.
AL805957, AL683877 Genomic DNA. Translation: CAM18851.1.
CH466538 Genomic DNA. Translation: EDL05585.1.
CH466538 Genomic DNA. Translation: EDL05587.1.
BC057926 mRNA. Translation: AAH57926.1.
CCDSiCCDS17989.1.
RefSeqiNP_062698.2. NM_019724.3.
XP_006537704.1. XM_006537641.1.
UniGeneiMm.187315.

Genome annotation databases

EnsembliENSMUST00000029881; ENSMUSP00000029881; ENSMUSG00000028226.
GeneIDi17389.
KEGGimmu:17389.
UCSCiuc008sbw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021228 mRNA. Translation: BAA78420.1 .
AF282844 mRNA. Translation: AAG17704.1 .
AL683877 , AL805957 Genomic DNA. Translation: CAM13981.1 .
AL805957 , AL683877 Genomic DNA. Translation: CAM18851.1 .
CH466538 Genomic DNA. Translation: EDL05585.1 .
CH466538 Genomic DNA. Translation: EDL05587.1 .
BC057926 mRNA. Translation: AAH57926.1 .
CCDSi CCDS17989.1.
RefSeqi NP_062698.2. NM_019724.3.
XP_006537704.1. XM_006537641.1.
UniGenei Mm.187315.

3D structure databases

ProteinModelPortali Q9WTR0.
SMRi Q9WTR0. Positions 85-561.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000029881.

Protein family/group databases

MEROPSi M10.016.

PTM databases

PhosphoSitei Q9WTR0.

Proteomic databases

PRIDEi Q9WTR0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029881 ; ENSMUSP00000029881 ; ENSMUSG00000028226 .
GeneIDi 17389.
KEGGi mmu:17389.
UCSCi uc008sbw.2. mouse.

Organism-specific databases

CTDi 4325.
MGIi MGI:1276107. Mmp16.

Phylogenomic databases

eggNOGi NOG295915.
GeneTreei ENSGT00750000117332.
HOGENOMi HOG000217928.
HOVERGENi HBG052484.
InParanoidi Q6PEQ6.
KOi K07996.
OMAi VPPHRSV.
OrthoDBi EOG7XPZ57.
TreeFami TF352396.

Enzyme and pathway databases

Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

Miscellaneous databases

ChiTaRSi MMP16. mouse.
NextBioi 292008.
PROi Q9WTR0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9WTR0.
Bgeei Q9WTR0.
CleanExi MM_MMP16.
Genevestigatori Q9WTR0.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028697. MMP16.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF26. PTHR10201:SF26. 1 hit.
Pfami PF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Seiki M., Kinoh H.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Differential localization and Triton X-100 solubility of membrane-type matrix metalloproteinases 1-3."
    Budde P., Gatsios P., Nienaber N.T., Li H., Staege M.S., Graeve L., Heinrich P.C., Frey J.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMMP16_MOUSE
AccessioniPrimary (citable) accession number: Q9WTR0
Secondary accession number(s): Q6PEQ6, Q9ERT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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