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Protein

Podocalyxin

Gene

Podxl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Podocalyxin
Alternative name(s):
Podocalyxin-like protein 1
Short name:
PC
Short name:
PCLP-1
Gene namesi
Name:Podxl
Synonyms:Pclp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi621878. Podxl.

Subcellular locationi

  • Apical cell membrane 1 Publication
  • Cell projectionmicrovillus 1 Publication
  • Membrane raft By similarity
  • Cell projectionlamellipodium By similarity
  • Cell projectionfilopodium By similarity
  • Cell projectionruffle By similarity
  • Membrane Curated; Single-pass type I membrane protein Curated

  • Note: In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form (By similarity). Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 386ExtracellularSequence analysisAdd BLAST362
Transmembranei387 – 407HelicalSequence analysisAdd BLAST21
Topological domaini408 – 485CytoplasmicSequence analysisAdd BLAST78

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • cell body Source: RGD
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • filopodium Source: UniProtKB
  • integral component of membrane Source: RGD
  • intracellular membrane-bounded organelle Source: Ensembl
  • lamellipodium Source: UniProtKB
  • membrane raft Source: UniProtKB-SubCell
  • microvillus membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • ruffle Source: UniProtKB
  • slit diaphragm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000002475725 – 485PodocalyxinAdd BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi144N-linked (GlcNAc...)Sequence analysis1
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Glycosylationi287N-linked (GlcNAc...)Sequence analysis1
Modified residuei445PhosphothreonineBy similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei483PhosphothreonineBy similarity1

Post-translational modificationi

N- and O-linked glycosylated. Sialoglycoprotein (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9WTQ2.
PRIDEiQ9WTQ2.

PTM databases

iPTMnetiQ9WTQ2.
PhosphoSitePlusiQ9WTQ2.

Expressioni

Tissue specificityi

Glomerular epithelium cell (podocyte) (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000012495.
ExpressionAtlasiQ9WTQ2. baseline and differential.
GenevisibleiQ9WTQ2. RN.

Interactioni

Subunit structurei

Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Interacts with SLC9A3R2. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R1 (via the PDZ domains); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2. Associates with the actin cytoskeleton through complex formation with EZR and SLC9A3R2. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R1 (via the PDZ domains); interaction is not detected in glomerular epithelium cells. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells. Interacts with EZR.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016991.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi58 – 265Ser/Thr-richAdd BLAST208

Domaini

Both the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with SLC9A3R1 and for targeting SLC9A3R1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity).By similarity

Sequence similaritiesi

Belongs to the podocalyxin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFKB. Eukaryota.
ENOG410YCG4. LUCA.
GeneTreeiENSGT00730000111314.
HOGENOMiHOG000115601.
HOVERGENiHBG053629.
InParanoidiQ9WTQ2.
KOiK06817.
OMAiYLNQEME.
OrthoDBiEOG091G0RL9.
PhylomeDBiQ9WTQ2.
TreeFamiTF333564.

Family and domain databases

InterProiIPR013836. CD34/Podocalyxin.
IPR017403. PODXL.
[Graphical view]
PANTHERiPTHR12067. PTHR12067. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PIRSFiPIRSF038143. Podocalyxin-like_p1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPTLALSAL LLLQLLLLST PSLSQDNGNK TDTSDITSID QNQDKPATNQ
60 70 80 90 100
PSNATPKSSV QPPTPTSIST SSPDPKATQS SNSSVTTTSD STTDRTSSST
110 120 130 140 150
STVPTTSNSG QTVSSGGKSS DKITTALPTT LGPVNASSQP TDLNTSTKLP
160 170 180 190 200
STPTTNSTAS PHQPVSHSEG QHTTVQSSSA SVSSSDNTTL LWILTTSKPT
210 220 230 240 250
GTSEGTQPIA ISTPGITTPV STPLQPTGSP GGTESVPTTE EFTHSTSSWT
260 270 280 290 300
PVVSQGPSTP SSTWTSGSYK LKCDPAIKPH EELLILNLTR DSFCKGSPPN
310 320 330 340 350
ERFLELLCHS AKASFKPAED SCALELAPIL DNQAVAVKRI VIETKLSPKA
360 370 380 390 400
VFELLKDKWD DLTEAGVIDI HLGKEGPPEV NEDRFSLPLI ITIVCMASFL
410 420 430 440 450
LLVAALYGCC HQRISQRKDQ QRLTEELQTV ENGYHDNPTL EVMETPSEMQ
460 470 480
EKKVVNLNGE LGDSWIVPLD NLTKEDLDEE EDTHL
Length:485
Mass (Da):51,658
Last modified:May 1, 2007 - v2
Checksum:i54088032631C774E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130T → S in BAA78375 (Ref. 1) Curated1
Sequence conflicti379E → G in BAA78375 (Ref. 1) Curated1
Sequence conflicti456N → S in BAA78375 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020726 mRNA. Translation: BAA78375.1.
AF109393 mRNA. Translation: AAF14238.1.
BC070886 mRNA. Translation: AAH70886.1.
RefSeqiNP_620203.1. NM_138848.1.
XP_008760980.1. XM_008762758.2.
XP_017447916.1. XM_017592427.1.
UniGeneiRn.162741.

Genome annotation databases

EnsembliENSRNOT00000016991; ENSRNOP00000016991; ENSRNOG00000012495.
ENSRNOT00000077406; ENSRNOP00000075234; ENSRNOG00000012495.
GeneIDi192181.
KEGGirno:192181.
UCSCiRGD:621878. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020726 mRNA. Translation: BAA78375.1.
AF109393 mRNA. Translation: AAF14238.1.
BC070886 mRNA. Translation: AAH70886.1.
RefSeqiNP_620203.1. NM_138848.1.
XP_008760980.1. XM_008762758.2.
XP_017447916.1. XM_017592427.1.
UniGeneiRn.162741.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016991.

PTM databases

iPTMnetiQ9WTQ2.
PhosphoSitePlusiQ9WTQ2.

Proteomic databases

PaxDbiQ9WTQ2.
PRIDEiQ9WTQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016991; ENSRNOP00000016991; ENSRNOG00000012495.
ENSRNOT00000077406; ENSRNOP00000075234; ENSRNOG00000012495.
GeneIDi192181.
KEGGirno:192181.
UCSCiRGD:621878. rat.

Organism-specific databases

CTDi5420.
RGDi621878. Podxl.

Phylogenomic databases

eggNOGiENOG410IFKB. Eukaryota.
ENOG410YCG4. LUCA.
GeneTreeiENSGT00730000111314.
HOGENOMiHOG000115601.
HOVERGENiHBG053629.
InParanoidiQ9WTQ2.
KOiK06817.
OMAiYLNQEME.
OrthoDBiEOG091G0RL9.
PhylomeDBiQ9WTQ2.
TreeFamiTF333564.

Miscellaneous databases

PROiQ9WTQ2.

Gene expression databases

BgeeiENSRNOG00000012495.
ExpressionAtlasiQ9WTQ2. baseline and differential.
GenevisibleiQ9WTQ2. RN.

Family and domain databases

InterProiIPR013836. CD34/Podocalyxin.
IPR017403. PODXL.
[Graphical view]
PANTHERiPTHR12067. PTHR12067. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PIRSFiPIRSF038143. Podocalyxin-like_p1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPODXL_RAT
AccessioniPrimary (citable) accession number: Q9WTQ2
Secondary accession number(s): Q6IRK7, Q9R068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.