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Protein

GTP:AMP phosphotransferase AK3, mitochondrial

Gene

Ak3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.UniRule annotation

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381AMPUniRule annotation
Binding sitei43 – 431AMPUniRule annotation
Binding sitei98 – 981AMPUniRule annotation
Binding sitei128 – 1281GTPUniRule annotation
Binding sitei161 – 1611AMPUniRule annotation
Binding sitei172 – 1721AMPUniRule annotation
Binding sitei201 – 2011GTP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 226GTPUniRule annotation
Nucleotide bindingi64 – 663AMPUniRule annotation
Nucleotide bindingi91 – 944AMPUniRule annotation
Nucleotide bindingi137 – 1382GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase AK3, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Adenylate kinase 3 alpha-like 1UniRule annotation
Gene namesi
Name:Ak3
Synonyms:Ak3l, Ak3l1, Akl3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1860835. Ak3.

Subcellular locationi

  • Mitochondrion matrix UniRule annotation

GO - Cellular componenti

  • mitochondrial matrix Source: BHF-UCL
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 227226GTP:AMP phosphotransferase AK3, mitochondrialPRO_0000158923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201N6-succinyllysineCombined sources
Modified residuei29 – 291N6-acetyllysine; alternateCombined sources
Modified residuei29 – 291N6-succinyllysine; alternateCombined sources
Modified residuei34 – 341N6-acetyllysineCombined sources
Modified residuei37 – 371PhosphoserineCombined sources
Modified residuei57 – 571N6-succinyllysineCombined sources
Modified residuei64 – 641N6-acetyllysine; alternateCombined sources
Modified residuei64 – 641N6-succinyllysine; alternateCombined sources
Modified residuei80 – 801N6-acetyllysine; alternateCombined sources
Modified residuei80 – 801N6-succinyllysine; alternateCombined sources
Modified residuei174 – 1741N6-acetyllysine; alternateCombined sources
Modified residuei174 – 1741N6-succinyllysine; alternateCombined sources
Modified residuei189 – 1891N6-acetyllysine; alternateCombined sources
Modified residuei189 – 1891N6-succinyllysine; alternateCombined sources
Modified residuei203 – 2031N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WTP7.
MaxQBiQ9WTP7.
PaxDbiQ9WTP7.
PeptideAtlasiQ9WTP7.
PRIDEiQ9WTP7.

PTM databases

iPTMnetiQ9WTP7.
PhosphoSiteiQ9WTP7.
SwissPalmiQ9WTP7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024782.
CleanExiMM_AK3.
MM_AK3L1.
ExpressionAtlasiQ9WTP7. baseline and differential.
GenevisibleiQ9WTP7. MM.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ9WTP7. 3 interactions.
MINTiMINT-1861667.
STRINGi10090.ENSMUSP00000025696.

Structurei

3D structure databases

ProteinModelPortaliQ9WTP7.
SMRiQ9WTP7. Positions 7-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 6630NMPbindUniRule annotationAdd
BLAST
Regioni127 – 16438LIDUniRule annotationAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiQ9WTP7.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
PhylomeDBiQ9WTP7.
TreeFamiTF312916.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASGRLLRA VIMGAPGSGK GTVSSRITKH FELKHLSSGD LLRQNMLQGT
60 70 80 90 100
EIGVLAKTFI DQGKLIPDDV MTRLALHELK TLTQCSWLLD GFPRTLPQAE
110 120 130 140 150
ALDKVYQIDT VINLNVPFEV IKQRLTARWI HPASGRVYNI EFNPPKTVGI
160 170 180 190 200
DDLTGEPLIQ REDDKPETVI KRLKAYEAQT EPVLQYYQKK GVLETFSGTE
210 220
TNKIWPHVYS FLQTKVPETT QKASVTP
Length:227
Mass (Da):25,426
Last modified:January 23, 2007 - v3
Checksum:i6601DF10971DE5AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti171 – 1711K → E in BAB23625 (PubMed:16141072).Curated
Sequence conflicti202 – 2021N → D in BAB23876 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004864 mRNA. Translation: BAB23625.1.
AK005194 mRNA. Translation: BAB23876.1.
AK008681 mRNA. Translation: BAB25829.1.
AK031631 mRNA. Translation: BAC27488.1.
AK053644 mRNA. Translation: BAC35459.1.
AK089027 mRNA. Translation: BAC40707.1.
AK147060 mRNA. Translation: BAE27644.1.
AK149996 mRNA. Translation: BAE29224.1.
BC016432 mRNA. Translation: AAH16432.1.
BC019174 mRNA. Translation: AAH19174.1.
BC024871 mRNA. Translation: AAH24871.1.
BC058191 mRNA. Translation: AAH58191.1.
AB020203 mRNA. Translation: BAA77360.1.
CCDSiCCDS29730.1.
RefSeqiNP_067274.1. NM_021299.1.
UniGeneiMm.196067.

Genome annotation databases

EnsembliENSMUST00000025696; ENSMUSP00000025696; ENSMUSG00000024782.
GeneIDi56248.
KEGGimmu:56248.
UCSCiuc008hcx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004864 mRNA. Translation: BAB23625.1.
AK005194 mRNA. Translation: BAB23876.1.
AK008681 mRNA. Translation: BAB25829.1.
AK031631 mRNA. Translation: BAC27488.1.
AK053644 mRNA. Translation: BAC35459.1.
AK089027 mRNA. Translation: BAC40707.1.
AK147060 mRNA. Translation: BAE27644.1.
AK149996 mRNA. Translation: BAE29224.1.
BC016432 mRNA. Translation: AAH16432.1.
BC019174 mRNA. Translation: AAH19174.1.
BC024871 mRNA. Translation: AAH24871.1.
BC058191 mRNA. Translation: AAH58191.1.
AB020203 mRNA. Translation: BAA77360.1.
CCDSiCCDS29730.1.
RefSeqiNP_067274.1. NM_021299.1.
UniGeneiMm.196067.

3D structure databases

ProteinModelPortaliQ9WTP7.
SMRiQ9WTP7. Positions 7-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WTP7. 3 interactions.
MINTiMINT-1861667.
STRINGi10090.ENSMUSP00000025696.

PTM databases

iPTMnetiQ9WTP7.
PhosphoSiteiQ9WTP7.
SwissPalmiQ9WTP7.

Proteomic databases

EPDiQ9WTP7.
MaxQBiQ9WTP7.
PaxDbiQ9WTP7.
PeptideAtlasiQ9WTP7.
PRIDEiQ9WTP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025696; ENSMUSP00000025696; ENSMUSG00000024782.
GeneIDi56248.
KEGGimmu:56248.
UCSCiuc008hcx.1. mouse.

Organism-specific databases

CTDi50808.
MGIiMGI:1860835. Ak3.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiQ9WTP7.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
PhylomeDBiQ9WTP7.
TreeFamiTF312916.

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiQ9WTP7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024782.
CleanExiMM_AK3.
MM_AK3L1.
ExpressionAtlasiQ9WTP7. baseline and differential.
GenevisibleiQ9WTP7. MM.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD3_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP7
Secondary accession number(s): Q3UDN7
, Q8BGX5, Q9D7Z1, Q9DB57, Q9DBM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.