Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenylate kinase 2, mitochondrial

Gene

Ak2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46AMPUniRule annotation1
Binding sitei51AMPUniRule annotation1
Binding sitei107AMPUniRule annotation1
Binding sitei142ATPUniRule annotation1
Binding sitei175AMPUniRule annotation1
Binding sitei186AMPUniRule annotation1
Binding sitei214ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPUniRule annotation6
Nucleotide bindingi72 – 74AMPUniRule annotation3
Nucleotide bindingi100 – 103AMPUniRule annotation4
Nucleotide bindingi151 – 152ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 2, mitochondrialUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK 2UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 2UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Cleaved into the following chain:
Gene namesi
Name:Ak2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87978. Ak2.

Subcellular locationi

  • Mitochondrion intermembrane space UniRule annotation

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • sperm flagellum Source: MGI
  • sperm mitochondrial sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589181 – 239Adenylate kinase 2, mitochondrialAdd BLAST239
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004343532 – 239Adenylate kinase 2, mitochondrial, N-terminally processedAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Disulfide bondi42 ↔ 92UniRule annotation
Modified residuei58PhosphoserineBy similarity1
Modified residuei62N6-succinyllysineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei93N6-succinyllysineCombined sources1
Modified residuei133PhosphoserineBy similarity1
Modified residuei181N6-acetyllysineCombined sources1
Modified residuei195PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9WTP6.
PeptideAtlasiQ9WTP6.
PRIDEiQ9WTP6.

2D gel databases

REPRODUCTION-2DPAGEQ9WTP6.

PTM databases

iPTMnetiQ9WTP6.
PhosphoSitePlusiQ9WTP6.
SwissPalmiQ9WTP6.

Expressioni

Tissue specificityi

Present in the inner ear. Not detected in the vestibule at any developmental stage. Present at high level in the cochlea uniquely in the stria vascularis at postnatal day 7 but not at birth. Present within the lumen of the stria vascularis capillaries. Not detected in the capillaries or vessels of the adjacent connective tissue (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000028792.
CleanExiMM_AK2.
ExpressionAtlasiQ9WTP6. baseline and differential.
GenevisibleiQ9WTP6. MM.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi198046. 1 interactor.
IntActiQ9WTP6. 4 interactors.
MINTiMINT-1859211.
STRINGi10090.ENSMUSP00000030583.

Structurei

3D structure databases

ProteinModelPortaliQ9WTP6.
SMRiQ9WTP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 74NMPbindUniRule annotationAdd BLAST30
Regioni141 – 178LIDUniRule annotationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOVERGENiHBG000458.
InParanoidiQ9WTP6.
KOiK00939.
OMAiCPKGIRA.
OrthoDBiEOG091G06BH.
TreeFamiTF300896.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPNVLASEP EIPKGIRAVL LGPPGAGKGT QAPKLAENFC VCHLATGDML
60 70 80 90 100
RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP SCKNGFLLDG
110 120 130 140 150
FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI QDSLLIRRIT GRLIHPKSGR
160 170 180 190 200
SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKTRLEAY HTQTTPLVEY
210 220 230
YRKRGIHCAI DASQTPDIVF ASILAAFSKA TCKDLVMFI
Length:239
Mass (Da):26,469
Last modified:March 3, 2009 - v5
Checksum:iCAFF06F053CECE36
GO
Isoform 2 (identifier: Q9WTP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     232-239: CKDLVMFI → S

Show »
Length:232
Mass (Da):25,606
Checksum:iEDD60400562323CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → R in BAA77359 (Ref. 1) Curated1
Sequence conflicti110M → V in BAE40113 (PubMed:16141072).Curated1
Sequence conflicti113D → E in BAA77359 (Ref. 1) Curated1
Sequence conflicti121K → E in BAA77359 (Ref. 1) Curated1
Sequence conflicti145H → R in BAA77359 (Ref. 1) Curated1
Sequence conflicti146P → R in BAE40113 (PubMed:16141072).Curated1
Sequence conflicti151S → F in BAA77359 (Ref. 1) Curated1
Sequence conflicti191H → Y in BAA77359 (Ref. 1) Curated1
Sequence conflicti204R → H in BAE40035 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036504232 – 239CKDLVMFI → S in isoform 2. 3 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020202 mRNA. Translation: BAA77359.1.
AK010951 mRNA. Translation: BAB27286.1.
AK050133 mRNA. Translation: BAC34085.1.
AK166976 mRNA. Translation: BAE39159.1.
AK168056 mRNA. Translation: BAE40035.1.
AK168148 mRNA. Translation: BAE40113.1.
AL607086 Genomic DNA. Translation: CAM19113.1.
AL607086 Genomic DNA. Translation: CAM19114.1.
CU210866 Genomic DNA. Translation: CAQ51906.1.
CU210866 Genomic DNA. Translation: CAQ51907.1.
BC008610 mRNA. Translation: AAH08610.1.
CCDSiCCDS18679.1. [Q9WTP6-2]
CCDS18680.1. [Q9WTP6-1]
RefSeqiNP_001029138.1. NM_001033966.4. [Q9WTP6-1]
NP_058591.2. NM_016895.4. [Q9WTP6-2]
UniGeneiMm.29460.

Genome annotation databases

EnsembliENSMUST00000030583; ENSMUSP00000030583; ENSMUSG00000028792. [Q9WTP6-1]
ENSMUST00000102604; ENSMUSP00000099664; ENSMUSG00000028792. [Q9WTP6-2]
GeneIDi11637.
KEGGimmu:11637.
UCSCiuc008uvt.2. mouse. [Q9WTP6-1]
uc008uvu.2. mouse. [Q9WTP6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020202 mRNA. Translation: BAA77359.1.
AK010951 mRNA. Translation: BAB27286.1.
AK050133 mRNA. Translation: BAC34085.1.
AK166976 mRNA. Translation: BAE39159.1.
AK168056 mRNA. Translation: BAE40035.1.
AK168148 mRNA. Translation: BAE40113.1.
AL607086 Genomic DNA. Translation: CAM19113.1.
AL607086 Genomic DNA. Translation: CAM19114.1.
CU210866 Genomic DNA. Translation: CAQ51906.1.
CU210866 Genomic DNA. Translation: CAQ51907.1.
BC008610 mRNA. Translation: AAH08610.1.
CCDSiCCDS18679.1. [Q9WTP6-2]
CCDS18680.1. [Q9WTP6-1]
RefSeqiNP_001029138.1. NM_001033966.4. [Q9WTP6-1]
NP_058591.2. NM_016895.4. [Q9WTP6-2]
UniGeneiMm.29460.

3D structure databases

ProteinModelPortaliQ9WTP6.
SMRiQ9WTP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198046. 1 interactor.
IntActiQ9WTP6. 4 interactors.
MINTiMINT-1859211.
STRINGi10090.ENSMUSP00000030583.

PTM databases

iPTMnetiQ9WTP6.
PhosphoSitePlusiQ9WTP6.
SwissPalmiQ9WTP6.

2D gel databases

REPRODUCTION-2DPAGEQ9WTP6.

Proteomic databases

PaxDbiQ9WTP6.
PeptideAtlasiQ9WTP6.
PRIDEiQ9WTP6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030583; ENSMUSP00000030583; ENSMUSG00000028792. [Q9WTP6-1]
ENSMUST00000102604; ENSMUSP00000099664; ENSMUSG00000028792. [Q9WTP6-2]
GeneIDi11637.
KEGGimmu:11637.
UCSCiuc008uvt.2. mouse. [Q9WTP6-1]
uc008uvu.2. mouse. [Q9WTP6-2]

Organism-specific databases

CTDi204.
MGIiMGI:87978. Ak2.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOVERGENiHBG000458.
InParanoidiQ9WTP6.
KOiK00939.
OMAiCPKGIRA.
OrthoDBiEOG091G06BH.
TreeFamiTF300896.

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

ChiTaRSiAk2. mouse.
PROiQ9WTP6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028792.
CleanExiMM_AK2.
ExpressionAtlasiQ9WTP6. baseline and differential.
GenevisibleiQ9WTP6. MM.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD2_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP6
Secondary accession number(s): A2A820
, Q3THT3, Q3TI11, Q3TKI6, Q8C7I9, Q9CY37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 149 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.