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Protein

Adenylate kinase 2, mitochondrial

Gene

Ak2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46AMPUniRule annotation1
Binding sitei51AMPUniRule annotation1
Binding sitei107AMPUniRule annotation1
Binding sitei142ATPUniRule annotation1
Binding sitei175AMPUniRule annotation1
Binding sitei186AMPUniRule annotation1
Binding sitei214ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPUniRule annotation6
Nucleotide bindingi72 – 74AMPUniRule annotation3
Nucleotide bindingi100 – 103AMPUniRule annotation4
Nucleotide bindingi151 – 152ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 2, mitochondrialUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK 2UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 2UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Gene namesi
Name:Ak2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87978 Ak2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589181 – 239Adenylate kinase 2, mitochondrialAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Disulfide bondi42 ↔ 92UniRule annotation
Modified residuei58PhosphoserineBy similarity1
Modified residuei62N6-succinyllysineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei93N6-succinyllysineCombined sources1
Modified residuei133PhosphoserineBy similarity1
Modified residuei181N6-acetyllysineCombined sources1
Modified residuei195PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9WTP6
PeptideAtlasiQ9WTP6
PRIDEiQ9WTP6

2D gel databases

REPRODUCTION-2DPAGEiQ9WTP6

PTM databases

iPTMnetiQ9WTP6
PhosphoSitePlusiQ9WTP6
SwissPalmiQ9WTP6

Expressioni

Tissue specificityi

Present in the inner ear. Not detected in the vestibule at any developmental stage. Present at high level in the cochlea uniquely in the stria vascularis at postnatal day 7 but not at birth. Present within the lumen of the stria vascularis capillaries. Not detected in the capillaries or vessels of the adjacent connective tissue (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000028792
CleanExiMM_AK2
ExpressionAtlasiQ9WTP6 baseline and differential
GenevisibleiQ9WTP6 MM

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi198046, 1 interactor
IntActiQ9WTP6, 4 interactors
MINTiQ9WTP6
STRINGi10090.ENSMUSP00000030583

Structurei

3D structure databases

ProteinModelPortaliQ9WTP6
SMRiQ9WTP6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 74NMPbindUniRule annotationAdd BLAST30
Regioni141 – 178LIDUniRule annotationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00830000128340
HOVERGENiHBG000458
InParanoidiQ9WTP6
KOiK00939
OMAiEKFTSQG
OrthoDBiEOG091G06BH
TreeFamiTF300896

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03168 Adenylate_kinase_AK2, 1 hit
InterProiView protein in InterPro
IPR006259 Adenyl_kin_sub
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR007862 Adenylate_kinase_lid-dom
IPR028587 AK2
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PfamiView protein in Pfam
PF05191 ADK_lid, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01351 adk, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPNVLASEP EIPKGIRAVL LGPPGAGKGT QAPKLAENFC VCHLATGDML
60 70 80 90 100
RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP SCKNGFLLDG
110 120 130 140 150
FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI QDSLLIRRIT GRLIHPKSGR
160 170 180 190 200
SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKTRLEAY HTQTTPLVEY
210 220 230
YRKRGIHCAI DASQTPDIVF ASILAAFSKA TCKDLVMFI
Length:239
Mass (Da):26,469
Last modified:March 3, 2009 - v5
Checksum:iCAFF06F053CECE36
GO
Isoform 2 (identifier: Q9WTP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     232-239: CKDLVMFI → S

Show »
Length:232
Mass (Da):25,606
Checksum:iEDD60400562323CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → R in BAA77359 (Ref. 1) Curated1
Sequence conflicti110M → V in BAE40113 (PubMed:16141072).Curated1
Sequence conflicti113D → E in BAA77359 (Ref. 1) Curated1
Sequence conflicti121K → E in BAA77359 (Ref. 1) Curated1
Sequence conflicti145H → R in BAA77359 (Ref. 1) Curated1
Sequence conflicti146P → R in BAE40113 (PubMed:16141072).Curated1
Sequence conflicti151S → F in BAA77359 (Ref. 1) Curated1
Sequence conflicti191H → Y in BAA77359 (Ref. 1) Curated1
Sequence conflicti204R → H in BAE40035 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036504232 – 239CKDLVMFI → S in isoform 2. 3 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020202 mRNA Translation: BAA77359.1
AK010951 mRNA Translation: BAB27286.1
AK050133 mRNA Translation: BAC34085.1
AK166976 mRNA Translation: BAE39159.1
AK168056 mRNA Translation: BAE40035.1
AK168148 mRNA Translation: BAE40113.1
AL607086 Genomic DNA No translation available.
CU210866 Genomic DNA No translation available.
BC008610 mRNA Translation: AAH08610.1
CCDSiCCDS18679.1 [Q9WTP6-2]
CCDS18680.1 [Q9WTP6-1]
RefSeqiNP_001029138.1, NM_001033966.4 [Q9WTP6-1]
NP_058591.2, NM_016895.4 [Q9WTP6-2]
UniGeneiMm.29460

Genome annotation databases

EnsembliENSMUST00000030583; ENSMUSP00000030583; ENSMUSG00000028792 [Q9WTP6-1]
ENSMUST00000102604; ENSMUSP00000099664; ENSMUSG00000028792 [Q9WTP6-2]
GeneIDi11637
KEGGimmu:11637
UCSCiuc008uvt.2 mouse [Q9WTP6-1]
uc008uvu.2 mouse [Q9WTP6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAD2_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP6
Secondary accession number(s): A2A820
, Q3THT3, Q3TI11, Q3TKI6, Q8C7I9, Q9CY37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 3, 2009
Last modified: February 28, 2018
This is version 157 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health