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Protein

SAM pointed domain-containing Ets transcription factor

Gene

Spdef

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi239 – 32284ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • intestinal epithelial cell development Source: MGI
  • lung goblet cell differentiation Source: MGI
  • negative regulation of cell fate commitment Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of cell fate commitment Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SAM pointed domain-containing Ets transcription factor
Alternative name(s):
Prostate epithelium-specific Ets transcription factor
Short name:
Prostate-specific Ets
Prostate-derived Ets factor
Gene namesi
Name:Spdef
Synonyms:Pdef, Pse
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1353422. Spdef.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325SAM pointed domain-containing Ets transcription factorPRO_0000223959Add
BLAST

Proteomic databases

PRIDEiQ9WTP3.

PTM databases

PhosphoSiteiQ9WTP3.

Expressioni

Tissue specificityi

Expressed in the accessory glands of sex organs including the prostate, seminal vesicle, coagulating gland in males, the oviduct in females, and in intestines. Expression is epithelial-specific.1 Publication

Gene expression databases

BgeeiQ9WTP3.
ExpressionAtlasiQ9WTP3. baseline and differential.
GenevisibleiQ9WTP3. MM.

Interactioni

Subunit structurei

Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi205953. 2 interactions.
STRINGi10090.ENSMUSP00000025054.

Structurei

3D structure databases

ProteinModelPortaliQ9WTP3.
SMRiQ9WTP3. Positions 121-203, 237-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 20385PNTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG243414.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237327.
HOVERGENiHBG080776.
InParanoidiQ9WTP3.
KOiK09442.
OMAiYPEDSSW.
OrthoDBiEOG76X60D.
PhylomeDBiQ9WTP3.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSASPGLSN VSPGCLLLFP DVAPRTGTEK AASGAMGPEK QEWSPSPPAT
60 70 80 90 100
PEQGLSAFYL SYFNMYPDDS SWVAKVPEAR AGEDHPEEPE QCPVIDSQAS
110 120 130 140 150
GSTLDEHSLE QVQSMVVGEV LKDIETACKL LNITADPGDW SPGNVQKWLL
160 170 180 190 200
WTEHQYRLPP AGKAFQELGG KELCAMSEEQ FRQRAPLGGD VLHAHLDIWK
210 220 230 240 250
SAAWMKERTS PGTLHYCAST SEEGWTDGEV DSSCSGQPIH LWQFLKELLL
260 270 280 290 300
KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGVRKNRPAM NYDKLSRSIR
310 320
QYYKKGIIRK PDISQRLVYQ FVHPV
Length:325
Mass (Da):36,356
Last modified:November 1, 1999 - v1
Checksum:iB55CF6CEC4501125
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019436 mRNA. Translation: BAA77329.1.
AK078863 mRNA. Translation: BAC37426.1.
BC012648 mRNA. Translation: AAH12648.1.
CCDSiCCDS28568.1.
RefSeqiNP_038919.1. NM_013891.4.
XP_006524442.1. XM_006524379.2.
XP_006524443.1. XM_006524380.2.
XP_006524444.1. XM_006524381.2.
XP_006536833.1. XM_006536770.2.
XP_006536834.1. XM_006536771.2.
XP_006536835.1. XM_006536772.2.
UniGeneiMm.26768.

Genome annotation databases

EnsembliENSMUST00000025054; ENSMUSP00000025054; ENSMUSG00000024215.
ENSMUST00000114870; ENSMUSP00000110520; ENSMUSG00000024215.
ENSMUST00000167489; ENSMUSP00000127056; ENSMUSG00000024215.
GeneIDi30051.
KEGGimmu:30051.
UCSCiuc008bpo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019436 mRNA. Translation: BAA77329.1.
AK078863 mRNA. Translation: BAC37426.1.
BC012648 mRNA. Translation: AAH12648.1.
CCDSiCCDS28568.1.
RefSeqiNP_038919.1. NM_013891.4.
XP_006524442.1. XM_006524379.2.
XP_006524443.1. XM_006524380.2.
XP_006524444.1. XM_006524381.2.
XP_006536833.1. XM_006536770.2.
XP_006536834.1. XM_006536771.2.
XP_006536835.1. XM_006536772.2.
UniGeneiMm.26768.

3D structure databases

ProteinModelPortaliQ9WTP3.
SMRiQ9WTP3. Positions 121-203, 237-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205953. 2 interactions.
STRINGi10090.ENSMUSP00000025054.

PTM databases

PhosphoSiteiQ9WTP3.

Proteomic databases

PRIDEiQ9WTP3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025054; ENSMUSP00000025054; ENSMUSG00000024215.
ENSMUST00000114870; ENSMUSP00000110520; ENSMUSG00000024215.
ENSMUST00000167489; ENSMUSP00000127056; ENSMUSG00000024215.
GeneIDi30051.
KEGGimmu:30051.
UCSCiuc008bpo.2. mouse.

Organism-specific databases

CTDi25803.
MGIiMGI:1353422. Spdef.

Phylogenomic databases

eggNOGiNOG243414.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237327.
HOVERGENiHBG080776.
InParanoidiQ9WTP3.
KOiK09442.
OMAiYPEDSSW.
OrthoDBiEOG76X60D.
PhylomeDBiQ9WTP3.
TreeFamiTF318679.

Miscellaneous databases

NextBioi307158.
PROiQ9WTP3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTP3.
ExpressionAtlasiQ9WTP3. baseline and differential.
GenevisibleiQ9WTP3. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the mouse Pse gene encoding a novel Ets family member."
    Yamada N., Tamai Y., Miyamoto H., Nozaki M.
    Gene 241:267-274(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Colon.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Colon.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiSPDEF_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1999
Last modified: July 22, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.