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Protein

Protein sprouty homolog 4

Gene

Spry4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras (By similarity). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (By similarity).By similarity

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. negative regulation of MAP kinase activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sprouty homolog 4
Short name:
Spry-4
Gene namesi
Name:Spry4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1345144. Spry4.

Subcellular locationi

Cytoplasm. Membrane; Peripheral membrane protein
Note: Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. focal adhesion Source: MGI
  3. Golgi apparatus Source: MGI
  4. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Protein sprouty homolog 4PRO_0000076906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei126 – 1261PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WTP2.
PRIDEiQ9WTP2.

PTM databases

PhosphoSiteiQ9WTP2.

Expressioni

Tissue specificityi

Expressed in the embryo and adult tissues including heart, brain, lung, kidney, and skeletal muscle.

Developmental stagei

At 8 dpc expressed in the lateral plate mesoderm of the primitive streak. At 9.5 and 10.5 dpc expressed in the nasal placodes, maxillary and mandibular processes, posterior part of the hyoid arch and the progress zone of the limb buds and the presomitic mesoderm. At 11.5 dpc expressed in the dorso-lateral region of the somites (mostly in the myotome) and in the otic vesicle. At 11.5 and 12.5 dpc expressed in the distal lung mesenchyme, with a strong expression in the accessory lobe of the lung.1 Publication

Inductioni

By FGF signaling.

Gene expression databases

BgeeiQ9WTP2.
CleanExiMM_SPRY4.
GenevestigatoriQ9WTP2.

Interactioni

Subunit structurei

Interacts with the C-terminus of TESK1. Interacts with RAF1.By similarity

Protein-protein interaction databases

BioGridi204878. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9WTP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini167 – 274108SPRPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 10811Poly-SerAdd
BLAST
Compositional biasi160 – 284125Cys-richAdd
BLAST

Domaini

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similaritiesi

Belongs to the sprouty family.Curated
Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG68509.
GeneTreeiENSGT00390000003535.
HOGENOMiHOG000233079.
HOVERGENiHBG003544.
InParanoidiQ9WTP2.
KOiK17385.
OMAiCCARWSF.
OrthoDBiEOG7G4QDV.
PhylomeDBiQ9WTP2.
TreeFamiTF325070.

Family and domain databases

InterProiIPR007875. Sprouty.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEiPS51227. SPR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTP2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPPVPQSSV PVNPSSVMVQ PLLDSRAPHS RLQHPLTILP IDQMKTSHVE
60 70 80 90 100
NDYIDNPSLA PATGPKRPRG GPPELAPTPA RCDQDITHHW ISFSGRPSSV
110 120 130 140 150
SSSSSTSSDQ RLLDHMAPPP VAEQASPRAV RLQPKVVHCK PLDLKGPTAP
160 170 180 190 200
PELDKHFLLC EACGKCKCKE CASPRTLPSC WVCNQECLCS AQTLVNYGTC
210 220 230 240 250
MCLVQGIFYH CTNEDDEGSC ADHPCSCSGS NCCARWSFMG ALSVVLPCLL
260 270 280 290 300
CYLPATGCVK LAQRGYDRLR RPGCRCKHTN SVICKAASGD TKTSRSDKPF
Length:300
Mass (Da):32,523
Last modified:November 1, 1999 - v1
Checksum:iDA963036EFC0E73F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101V → F in AAD56007. (PubMed:10498682)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019280 mRNA. Translation: BAA77689.1.
AF176906 mRNA. Translation: AAD56007.1.
AK037180 mRNA. Translation: BAC29739.1.
AK048122 mRNA. Translation: BAC33249.1.
CH466528 Genomic DNA. Translation: EDL10072.1.
BC050944 mRNA. Translation: AAH50944.1.
BC057005 mRNA. Translation: AAH57005.1.
CCDSiCCDS29202.1.
RefSeqiNP_036028.2. NM_011898.2.
UniGeneiMm.42038.

Genome annotation databases

EnsembliENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427.
GeneIDi24066.
KEGGimmu:24066.
UCSCiuc008esl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019280 mRNA. Translation: BAA77689.1.
AF176906 mRNA. Translation: AAD56007.1.
AK037180 mRNA. Translation: BAC29739.1.
AK048122 mRNA. Translation: BAC33249.1.
CH466528 Genomic DNA. Translation: EDL10072.1.
BC050944 mRNA. Translation: AAH50944.1.
BC057005 mRNA. Translation: AAH57005.1.
CCDSiCCDS29202.1.
RefSeqiNP_036028.2. NM_011898.2.
UniGeneiMm.42038.

3D structure databases

ProteinModelPortaliQ9WTP2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204878. 7 interactions.

PTM databases

PhosphoSiteiQ9WTP2.

Proteomic databases

MaxQBiQ9WTP2.
PRIDEiQ9WTP2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427.
GeneIDi24066.
KEGGimmu:24066.
UCSCiuc008esl.1. mouse.

Organism-specific databases

CTDi81848.
MGIiMGI:1345144. Spry4.

Phylogenomic databases

eggNOGiNOG68509.
GeneTreeiENSGT00390000003535.
HOGENOMiHOG000233079.
HOVERGENiHBG003544.
InParanoidiQ9WTP2.
KOiK17385.
OMAiCCARWSF.
OrthoDBiEOG7G4QDV.
PhylomeDBiQ9WTP2.
TreeFamiTF325070.

Miscellaneous databases

NextBioi304037.
PROiQ9WTP2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTP2.
CleanExiMM_SPRY4.
GenevestigatoriQ9WTP2.

Family and domain databases

InterProiIPR007875. Sprouty.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEiPS51227. SPR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression pattern of a mouse homologue of Drosophila sprouty in the mouse embryo."
    de Maximy A.A., Nakatake Y., Moncada S., Itoh N., Thiery J.P., Bellusci S.
    Mech. Dev. 81:213-216(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  2. "Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed."
    Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R.
    Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Skin.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiSPY4_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP2
Secondary accession number(s): Q543R1, Q9QXV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: February 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.