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Q9WTP2 (SPY4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein sprouty homolog 4

Short name=Spry-4
Gene names
Name:Spry4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras By similarity. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 By similarity.

Subunit structure

Interacts with the C-terminus of TESK1 By similarity. Interacts with RAF1 By similarity.

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein. Note: Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Tissue specificity

Expressed in the embryo and adult tissues including heart, brain, lung, kidney, and skeletal muscle.

Developmental stage

At 8 dpc expressed in the lateral plate mesoderm of the primitive streak. At 9.5 and 10.5 dpc expressed in the nasal placodes, maxillary and mandibular processes, posterior part of the hyoid arch and the progress zone of the limb buds and the presomitic mesoderm. At 11.5 dpc expressed in the dorso-lateral region of the somites (mostly in the myotome) and in the otic vesicle. At 11.5 and 12.5 dpc expressed in the distal lung mesenchyme, with a strong expression in the accessory lobe of the lung. Ref.1

Induction

By FGF signaling.

Domain

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similarities

Belongs to the sprouty family.

Contains 1 SPR (sprouty) domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Protein sprouty homolog 4
PRO_0000076906

Regions

Domain167 – 274108SPR
Compositional bias98 – 10811Poly-Ser
Compositional bias160 – 284125Cys-rich

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1261Phosphoserine By similarity

Experimental info

Sequence conflict101V → F in AAD56007. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WTP2 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: DA963036EFC0E73F

FASTA30032,523
        10         20         30         40         50         60 
MEPPVPQSSV PVNPSSVMVQ PLLDSRAPHS RLQHPLTILP IDQMKTSHVE NDYIDNPSLA 

        70         80         90        100        110        120 
PATGPKRPRG GPPELAPTPA RCDQDITHHW ISFSGRPSSV SSSSSTSSDQ RLLDHMAPPP 

       130        140        150        160        170        180 
VAEQASPRAV RLQPKVVHCK PLDLKGPTAP PELDKHFLLC EACGKCKCKE CASPRTLPSC 

       190        200        210        220        230        240 
WVCNQECLCS AQTLVNYGTC MCLVQGIFYH CTNEDDEGSC ADHPCSCSGS NCCARWSFMG 

       250        260        270        280        290        300 
ALSVVLPCLL CYLPATGCVK LAQRGYDRLR RPGCRCKHTN SVICKAASGD TKTSRSDKPF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression pattern of a mouse homologue of Drosophila sprouty in the mouse embryo."
de Maximy A.A., Nakatake Y., Moncada S., Itoh N., Thiery J.P., Bellusci S.
Mech. Dev. 81:213-216(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]"Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed."
Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R.
Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head and Skin.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB019280 mRNA. Translation: BAA77689.1.
AF176906 mRNA. Translation: AAD56007.1.
AK037180 mRNA. Translation: BAC29739.1.
AK048122 mRNA. Translation: BAC33249.1.
CH466528 Genomic DNA. Translation: EDL10072.1.
BC050944 mRNA. Translation: AAH50944.1.
BC057005 mRNA. Translation: AAH57005.1.
RefSeqNP_036028.2. NM_011898.2.
UniGeneMm.42038.

3D structure databases

ProteinModelPortalQ9WTP2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204878. 7 interactions.

PTM databases

PhosphoSiteQ9WTP2.

Proteomic databases

PRIDEQ9WTP2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427.
GeneID24066.
KEGGmmu:24066.
UCSCuc008esl.1. mouse.

Organism-specific databases

CTD81848.
MGIMGI:1345144. Spry4.

Phylogenomic databases

eggNOGNOG68509.
GeneTreeENSGT00390000003535.
HOGENOMHOG000233079.
HOVERGENHBG003544.
InParanoidQ543R1.
KOK17385.
OMACCARWSF.
OrthoDBEOG7G4QDV.
PhylomeDBQ9WTP2.
TreeFamTF325070.

Gene expression databases

BgeeQ9WTP2.
CleanExMM_SPRY4.
GenevestigatorQ9WTP2.

Family and domain databases

InterProIPR007875. Sprouty.
[Graphical view]
PfamPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEPS51227. SPR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304037.
PROQ9WTP2.
SOURCESearch...

Entry information

Entry nameSPY4_MOUSE
AccessionPrimary (citable) accession number: Q9WTP2
Secondary accession number(s): Q543R1, Q9QXV7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot