Q9WTP2 (SPY4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein sprouty homolog 4
Short name=Spry-4

Gene names

Name:

Spry4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras By similarity. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 By similarity.
Subunit structure	Interacts with the C-terminus of TESK1 By similarity. Interacts with RAF1 By similarity.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).
Tissue specificity	Expressed in the embryo and adult tissues including heart, brain, lung, kidney, and skeletal muscle.
Developmental stage	At 8 dpc expressed in the lateral plate mesoderm of the primitive streak. At 9.5 and 10.5 dpc expressed in the nasal placodes, maxillary and mandibular processes, posterior part of the hyoid arch and the progress zone of the limb buds and the presomitic mesoderm. At 11.5 dpc expressed in the dorso-lateral region of the somites (mostly in the myotome) and in the otic vesicle. At 11.5 and 12.5 dpc expressed in the distal lung mesenchyme, with a strong expression in the accessory lobe of the lung. Ref.1
Induction	By FGF signaling.
Domain	The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Sequence similarities	Belongs to the sprouty family. Contains 1 SPR (sprouty) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of MAP kinase activity Inferred from direct assay PubMed 16339969. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Protein sprouty homolog 4		PRO_0000076906
Regions
	Domain	167 – 274	108	SPR
	Compositional bias	98 – 108	11	Poly-Ser
	Compositional bias	160 – 284	125	Cys-rich
Amino acid modifications
	Modified residue	126	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	10	1	V → F in AAD56007. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9WTP2 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: DA963036EFC0E73F	FASTA	300	32,523
	10 20 30 40 50 60 MEPPVPQSSV PVNPSSVMVQ PLLDSRAPHS RLQHPLTILP IDQMKTSHVE NDYIDNPSLA 70 80 90 100 110 120 PATGPKRPRG GPPELAPTPA RCDQDITHHW ISFSGRPSSV SSSSSTSSDQ RLLDHMAPPP 130 140 150 160 170 180 VAEQASPRAV RLQPKVVHCK PLDLKGPTAP PELDKHFLLC EACGKCKCKE CASPRTLPSC 190 200 210 220 230 240 WVCNQECLCS AQTLVNYGTC MCLVQGIFYH CTNEDDEGSC ADHPCSCSGS NCCARWSFMG 250 260 270 280 290 300 ALSVVLPCLL CYLPATGCVK LAQRGYDRLR RPGCRCKHTN SVICKAASGD TKTSRSDKPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression pattern of a mouse homologue of Drosophila sprouty in the mouse embryo." de Maximy A.A., Nakatake Y., Moncada S., Itoh N., Thiery J.P., Bellusci S. Mech. Dev. 81:213-216(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed." Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R. Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head and Skin.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019280 mRNA. Translation: BAA77689.1. AF176906 mRNA. Translation: AAD56007.1. AK037180 mRNA. Translation: BAC29739.1. AK048122 mRNA. Translation: BAC33249.1. CH466528 Genomic DNA. Translation: EDL10072.1. BC050944 mRNA. Translation: AAH50944.1. BC057005 mRNA. Translation: AAH57005.1.
IPI	IPI00322677.
RefSeq	NP_036028.2. NM_011898.2.
UniGene	Mm.42038.
3D structure databases
ProteinModelPortal	Q9WTP2.
ModBase	Search...
PTM databases
PhosphoSite	Q9WTP2.
Proteomic databases
PRIDE	Q9WTP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427.
GeneID	24066.
KEGG	mmu:24066.
UCSC	uc008esl.1. mouse.
Organism-specific databases
CTD	81848.
MGI	MGI:1345144. Spry4.
Phylogenomic databases
eggNOG	NOG68509.
GeneTree	ENSGT00390000003535.
HOGENOM	HOG000233079.
HOVERGEN	HBG003544.
InParanoid	Q543R1.
KO	K04704.
OMA	CCARWSF.
OrthoDB	EOG4THVTX.
Gene expression databases
Bgee	Q9WTP2.
CleanEx	MM_SPRY4.
Genevestigator	Q9WTP2.
GermOnline	ENSMUSG00000024427. Mus musculus.
Family and domain databases
InterPro	IPR007875. Sprouty. [Graphical view]
Pfam	PF05210. Sprouty. 1 hit. [Graphical view]
PROSITE	PS51227. SPR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304037.
SOURCE	Search...

Entry information

Entry name

SPY4_MOUSE

Accession

Primary (citable) accession number: Q9WTP2
Secondary accession number(s): Q543R1, Q9QXV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program