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Q9WTP2

- SPY4_MOUSE

UniProt

Q9WTP2 - SPY4_MOUSE

Protein

Protein sprouty homolog 4

Gene

Spry4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras By similarity. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. negative regulation of MAP kinase activity Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein sprouty homolog 4
    Short name:
    Spry-4
    Gene namesi
    Name:Spry4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1345144. Spry4.

    Subcellular locationi

    Cytoplasm. Membrane; Peripheral membrane protein
    Note: Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Protein sprouty homolog 4PRO_0000076906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei126 – 1261PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ9WTP2.

    PTM databases

    PhosphoSiteiQ9WTP2.

    Expressioni

    Tissue specificityi

    Expressed in the embryo and adult tissues including heart, brain, lung, kidney, and skeletal muscle.

    Developmental stagei

    At 8 dpc expressed in the lateral plate mesoderm of the primitive streak. At 9.5 and 10.5 dpc expressed in the nasal placodes, maxillary and mandibular processes, posterior part of the hyoid arch and the progress zone of the limb buds and the presomitic mesoderm. At 11.5 dpc expressed in the dorso-lateral region of the somites (mostly in the myotome) and in the otic vesicle. At 11.5 and 12.5 dpc expressed in the distal lung mesenchyme, with a strong expression in the accessory lobe of the lung.1 Publication

    Inductioni

    By FGF signaling.

    Gene expression databases

    BgeeiQ9WTP2.
    CleanExiMM_SPRY4.
    GenevestigatoriQ9WTP2.

    Interactioni

    Subunit structurei

    Interacts with the C-terminus of TESK1. Interacts with RAF1.By similarity

    Protein-protein interaction databases

    BioGridi204878. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WTP2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini167 – 274108SPRPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi98 – 10811Poly-SerAdd
    BLAST
    Compositional biasi160 – 284125Cys-richAdd
    BLAST

    Domaini

    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

    Sequence similaritiesi

    Belongs to the sprouty family.Curated
    Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG68509.
    GeneTreeiENSGT00390000003535.
    HOGENOMiHOG000233079.
    HOVERGENiHBG003544.
    InParanoidiQ543R1.
    KOiK17385.
    OMAiCCARWSF.
    OrthoDBiEOG7G4QDV.
    PhylomeDBiQ9WTP2.
    TreeFamiTF325070.

    Family and domain databases

    InterProiIPR007875. Sprouty.
    [Graphical view]
    PfamiPF05210. Sprouty. 1 hit.
    [Graphical view]
    PROSITEiPS51227. SPR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WTP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPPVPQSSV PVNPSSVMVQ PLLDSRAPHS RLQHPLTILP IDQMKTSHVE    50
    NDYIDNPSLA PATGPKRPRG GPPELAPTPA RCDQDITHHW ISFSGRPSSV 100
    SSSSSTSSDQ RLLDHMAPPP VAEQASPRAV RLQPKVVHCK PLDLKGPTAP 150
    PELDKHFLLC EACGKCKCKE CASPRTLPSC WVCNQECLCS AQTLVNYGTC 200
    MCLVQGIFYH CTNEDDEGSC ADHPCSCSGS NCCARWSFMG ALSVVLPCLL 250
    CYLPATGCVK LAQRGYDRLR RPGCRCKHTN SVICKAASGD TKTSRSDKPF 300
    Length:300
    Mass (Da):32,523
    Last modified:November 1, 1999 - v1
    Checksum:iDA963036EFC0E73F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101V → F in AAD56007. (PubMed:10498682)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019280 mRNA. Translation: BAA77689.1.
    AF176906 mRNA. Translation: AAD56007.1.
    AK037180 mRNA. Translation: BAC29739.1.
    AK048122 mRNA. Translation: BAC33249.1.
    CH466528 Genomic DNA. Translation: EDL10072.1.
    BC050944 mRNA. Translation: AAH50944.1.
    BC057005 mRNA. Translation: AAH57005.1.
    CCDSiCCDS29202.1.
    RefSeqiNP_036028.2. NM_011898.2.
    UniGeneiMm.42038.

    Genome annotation databases

    EnsembliENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427.
    GeneIDi24066.
    KEGGimmu:24066.
    UCSCiuc008esl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019280 mRNA. Translation: BAA77689.1 .
    AF176906 mRNA. Translation: AAD56007.1 .
    AK037180 mRNA. Translation: BAC29739.1 .
    AK048122 mRNA. Translation: BAC33249.1 .
    CH466528 Genomic DNA. Translation: EDL10072.1 .
    BC050944 mRNA. Translation: AAH50944.1 .
    BC057005 mRNA. Translation: AAH57005.1 .
    CCDSi CCDS29202.1.
    RefSeqi NP_036028.2. NM_011898.2.
    UniGenei Mm.42038.

    3D structure databases

    ProteinModelPortali Q9WTP2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204878. 7 interactions.

    PTM databases

    PhosphoSitei Q9WTP2.

    Proteomic databases

    PRIDEi Q9WTP2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025295 ; ENSMUSP00000025295 ; ENSMUSG00000024427 .
    GeneIDi 24066.
    KEGGi mmu:24066.
    UCSCi uc008esl.1. mouse.

    Organism-specific databases

    CTDi 81848.
    MGIi MGI:1345144. Spry4.

    Phylogenomic databases

    eggNOGi NOG68509.
    GeneTreei ENSGT00390000003535.
    HOGENOMi HOG000233079.
    HOVERGENi HBG003544.
    InParanoidi Q543R1.
    KOi K17385.
    OMAi CCARWSF.
    OrthoDBi EOG7G4QDV.
    PhylomeDBi Q9WTP2.
    TreeFami TF325070.

    Miscellaneous databases

    NextBioi 304037.
    PROi Q9WTP2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WTP2.
    CleanExi MM_SPRY4.
    Genevestigatori Q9WTP2.

    Family and domain databases

    InterProi IPR007875. Sprouty.
    [Graphical view ]
    Pfami PF05210. Sprouty. 1 hit.
    [Graphical view ]
    PROSITEi PS51227. SPR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression pattern of a mouse homologue of Drosophila sprouty in the mouse embryo."
      de Maximy A.A., Nakatake Y., Moncada S., Itoh N., Thiery J.P., Bellusci S.
      Mech. Dev. 81:213-216(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    2. "Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed."
      Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R.
      Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head and Skin.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiSPY4_MOUSE
    AccessioniPrimary (citable) accession number: Q9WTP2
    Secondary accession number(s): Q543R1, Q9QXV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3