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Protein

Two pore calcium channel protein 1

Gene

Tpcn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca2+ channels (VDCC) across the lysosomal and endosomal membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Two pore calcium channel protein 1
Alternative name(s):
Voltage-dependent calcium channel protein TPC1
Gene namesi
Name:Tpcn1
Synonyms:Tpc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708494. Tpcn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 101101CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221Helical; Name=S1 of repeat ISequence analysisAdd
BLAST
Topological domaini123 – 13715ExtracellularSequence analysisAdd
BLAST
Transmembranei138 – 15821Helical; Name=S2 of repeat ISequence analysisAdd
BLAST
Topological domaini159 – 17214CytoplasmicSequence analysisAdd
BLAST
Transmembranei173 – 19321Helical; Name=S3 of repeat ISequence analysisAdd
BLAST
Topological domaini194 – 2029ExtracellularSequence analysis
Transmembranei203 – 22119Helical; Name=S4 of repeat ISequence analysisAdd
BLAST
Topological domaini222 – 23514CytoplasmicSequence analysisAdd
BLAST
Transmembranei236 – 25621Helical; Name=S5 of repeat ISequence analysisAdd
BLAST
Topological domaini257 – 2637ExtracellularSequence analysis
Intramembranei264 – 28724Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini288 – 29811ExtracellularSequence analysisAdd
BLAST
Transmembranei299 – 31921Helical; Name=S6 of repeat ISequence analysisAdd
BLAST
Topological domaini320 – 445126CytoplasmicSequence analysisAdd
BLAST
Transmembranei446 – 46621Helical; Name=S1 of repeat IISequence analysisAdd
BLAST
Topological domaini467 – 48014ExtracellularSequence analysisAdd
BLAST
Transmembranei481 – 50121Helical; Name=S2 of repeat IISequence analysisAdd
BLAST
Topological domaini502 – 5043CytoplasmicSequence analysis
Transmembranei505 – 52723Helical; Name=S3 of repeat IISequence analysisAdd
BLAST
Topological domaini528 – 5358ExtracellularSequence analysis
Transmembranei536 – 55015Helical; Name=S4 of repeat IISequence analysisAdd
BLAST
Topological domaini551 – 57424CytoplasmicSequence analysisAdd
BLAST
Transmembranei575 – 59521Helical; Name=S5 of repeat IISequence analysisAdd
BLAST
Topological domaini596 – 63035ExtracellularSequence analysisAdd
BLAST
Intramembranei631 – 65424Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini655 – 67117ExtracellularSequence analysisAdd
BLAST
Transmembranei672 – 69221Helical; Name=S6 of repeat IISequence analysisAdd
BLAST
Topological domaini693 – 817125CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 817817Two pore calcium channel protein 1PRO_0000276855Add
BLAST

Proteomic databases

PRIDEiQ9WTN5.

PTM databases

PhosphoSiteiQ9WTN5.

Expressioni

Tissue specificityi

Widely expressed. Expressed at relatively high level in kidney, liver and lung, and in the kidney it is expressed at inner medullary collecting ducts.1 Publication

Interactioni

Subunit structurei

Dimer.Curated

Structurei

3D structure databases

ProteinModelPortaliQ9WTN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili770 – 79425Sequence analysisAdd
BLAST

Domaini

Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000006773.
HOVERGENiHBG080605.
InParanoidiQ9WTN5.
KOiK16896.
PhylomeDBiQ9WTN5.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WTN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVILDDDVL LILTLDEELS APLTPSNGLG QEDLPSKNGG GQSGPNSQVP
60 70 80 90 100
SLVSGADSPP SSPPGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA
110 120 130 140 150
YLFVHNHFFY MMELLTALLL LLLSLCESPA VPALKLRTYV HATLELFALM
160 170 180 190 200
VVVFELCMKL RWLGFHTFVR HKRTMVKTSV LVVQFIEAIV VLVRQTSHVR
210 220 230 240 250
VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL LFFMIIFAIL
260 270 280 290 300
GFYLFSTNPS DPYFNTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF
310 320 330 340 350
FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYHLL
360 370 380 390 400
VSQRRPAGIS YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF
410 420 430 440 450
YDIYEVAALQ WKAKKNRQHW FDELPRTAFL IFKGINILVN SKAFQYFMYL
460 470 480 490 500
VVAVNGVWIL VETFMLKGGN FISKHVPWSY LVFLTIYGVE LFMKVAGLGP
510 520 530 540 550
VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR PLQLLRLFKL
560 570 580 590 600
KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF SGRLSPNCCN
610 620 630 640 650
SSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW
660 670 680 690 700
YIIMEGVTSQ TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK
710 720 730 740 750
SQESEVDSGI VIEKEMSKEE LLAILELHRE ARGTSSDVTR LLDTLSQMEK
760 770 780 790 800
YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE WYEEHAREQE QQQLRGSAPS
810
PAAQQTPGSR QRSQTVT
Length:817
Mass (Da):94,405
Last modified:October 11, 2005 - v2
Checksum:i0676FCF9D130DA46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018253 mRNA. Translation: BAA76556.2.
BC062072 mRNA. Translation: AAH62072.1.
PIRiJC7240.
RefSeqiNP_647548.2. NM_139332.3.
UniGeneiRn.24484.

Genome annotation databases

GeneIDi246215.
KEGGirno:246215.
UCSCiRGD:708494. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018253 mRNA. Translation: BAA76556.2.
BC062072 mRNA. Translation: AAH62072.1.
PIRiJC7240.
RefSeqiNP_647548.2. NM_139332.3.
UniGeneiRn.24484.

3D structure databases

ProteinModelPortaliQ9WTN5.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9WTN5.

Proteomic databases

PRIDEiQ9WTN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246215.
KEGGirno:246215.
UCSCiRGD:708494. rat.

Organism-specific databases

CTDi53373.
RGDi708494. Tpcn1.

Phylogenomic databases

HOGENOMiHOG000006773.
HOVERGENiHBG080605.
InParanoidiQ9WTN5.
KOiK16896.
PhylomeDBiQ9WTN5.

Miscellaneous databases

NextBioi623499.
PROiQ9WTN5.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels."
    Ishibashi K., Suzuki M., Imai M.
    Biochem. Biophys. Res. Commun. 270:370-376(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, TISSUE SPECIFICITY.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiTPC1_RAT
AccessioniPrimary (citable) accession number: Q9WTN5
Secondary accession number(s): Q6P6R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 11, 2005
Last modified: May 11, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.