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Protein

RuvB-like 2

Gene

Ruvbl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.By similarity
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity).By similarity
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.By similarity
Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2 (By similarity).By similarity
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 848ATPBy similarity

GO - Molecular functioni

  • ADP binding Source: UniProtKB
  • ATPase binding Source: UniProtKB
  • ATP binding Source: UniProtKB
  • ATP-dependent 5'-3' DNA helicase activity Source: InterPro
  • chromatin DNA binding Source: MGI
  • damaged DNA binding Source: InterPro
  • DNA helicase activity Source: MGI
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: UniProtKB
  • RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
  • TFIID-class transcription factor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RuvB-like 2 (EC:3.6.4.12)
Alternative name(s):
p47 protein
Gene namesi
Name:Ruvbl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1342299. Ruvbl2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 463462RuvB-like 2PRO_0000165645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WTM5.
MaxQBiQ9WTM5.
PaxDbiQ9WTM5.
PRIDEiQ9WTM5.

2D gel databases

REPRODUCTION-2DPAGEQ9WTM5.

PTM databases

iPTMnetiQ9WTM5.
PhosphoSiteiQ9WTM5.

Expressioni

Gene expression databases

BgeeiQ9WTM5.
CleanExiMM_RUVBL2.
ExpressionAtlasiQ9WTM5. baseline and differential.
GenevisibleiQ9WTM5. MM.

Interactioni

Subunit structurei

Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C-terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Interacts with HINT1. Component of a SWR1-like complex. Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3. Interacts with ITFG1.By similarityCurated

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: UniProtKB
  • TFIID-class transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203037. 64 interactions.
IntActiQ9WTM5. 68 interactions.
MINTiMINT-4133502.
STRINGi10090.ENSMUSP00000033087.

Structurei

3D structure databases

ProteinModelPortaliQ9WTM5.
SMRiQ9WTM5. Positions 8-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.Curated

Phylogenomic databases

eggNOGiKOG2680. Eukaryota.
COG1224. LUCA.
GeneTreeiENSGT00550000075034.
HOGENOMiHOG000190885.
HOVERGENiHBG054186.
InParanoidiQ9WTM5.
KOiK11338.
OMAiYDAMGAQ.
OrthoDBiEOG75TMBR.
PhylomeDBiQ9WTM5.
TreeFamiTF300469.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiPF06068. TIP49. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVAATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL
60 70 80 90 100
AARRAAGVVL EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF
110 120 130 140 150
TAIAGSEIFS LEMSKTEALT QAFRRSIGVR IKEETEIIEG EVVEIQIDRP
160 170 180 190 200
ATGTGSKVGK LTLKTTEMET IYDLGTKMIE SLTKDKVQAG DVITIDKATG
210 220 230 240 250
KISKLGRSFT RARDYDAMGS QTKFVQCPDG ELQKRKEVVH TVSLHEIDVI
260 270 280 290 300
NSRTQGFLAL FSGDTGEIKS EVREQINAKV AEWREEGKAE IIPGVLFIDE
310 320 330 340 350
VHMLDIESFS FLNRALESDM APVLIMATNR GITRIRGTSY QSPHGIPIDL
360 370 380 390 400
LDRLLIVSTS PYSEKDTKQI LRIRCEEEDV EMSEDAYTVL TRIGLETSLR
410 420 430 440 450
YAIQLITAAS LVCRKRKGTE VQVDDIKRVY SLFLDESRST QYMKEYQDAF
460
LFNELKGETM DTS
Length:463
Mass (Da):51,113
Last modified:January 23, 2007 - v3
Checksum:i419D779972933308
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013912 mRNA. Translation: BAA76297.1.
CCDSiCCDS21243.1.
RefSeqiNP_035434.1. NM_011304.3.
XP_006540785.1. XM_006540722.1.
XP_006540786.1. XM_006540723.2.
UniGeneiMm.34410.

Genome annotation databases

EnsembliENSMUST00000107771; ENSMUSP00000103400; ENSMUSG00000003868.
GeneIDi20174.
KEGGimmu:20174.
UCSCiuc009gvc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013912 mRNA. Translation: BAA76297.1.
CCDSiCCDS21243.1.
RefSeqiNP_035434.1. NM_011304.3.
XP_006540785.1. XM_006540722.1.
XP_006540786.1. XM_006540723.2.
UniGeneiMm.34410.

3D structure databases

ProteinModelPortaliQ9WTM5.
SMRiQ9WTM5. Positions 8-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203037. 64 interactions.
IntActiQ9WTM5. 68 interactions.
MINTiMINT-4133502.
STRINGi10090.ENSMUSP00000033087.

PTM databases

iPTMnetiQ9WTM5.
PhosphoSiteiQ9WTM5.

2D gel databases

REPRODUCTION-2DPAGEQ9WTM5.

Proteomic databases

EPDiQ9WTM5.
MaxQBiQ9WTM5.
PaxDbiQ9WTM5.
PRIDEiQ9WTM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107771; ENSMUSP00000103400; ENSMUSG00000003868.
GeneIDi20174.
KEGGimmu:20174.
UCSCiuc009gvc.1. mouse.

Organism-specific databases

CTDi10856.
MGIiMGI:1342299. Ruvbl2.

Phylogenomic databases

eggNOGiKOG2680. Eukaryota.
COG1224. LUCA.
GeneTreeiENSGT00550000075034.
HOGENOMiHOG000190885.
HOVERGENiHBG054186.
InParanoidiQ9WTM5.
KOiK11338.
OMAiYDAMGAQ.
OrthoDBiEOG75TMBR.
PhylomeDBiQ9WTM5.
TreeFamiTF300469.

Miscellaneous databases

PROiQ9WTM5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTM5.
CleanExiMM_RUVBL2.
ExpressionAtlasiQ9WTM5. baseline and differential.
GenevisibleiQ9WTM5. MM.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiPF06068. TIP49. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of mouse p47, a second group mammalian RuvB DNA helicase-like protein: homology with those from human and Saccharomyces cerevisiae."
    Gohshi T., Simada M., Kawahire S., Imai N., Ichimura T., Omata S., Horigome T.
    J. Biochem. 125:939-946(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiRUVB2_MOUSE
AccessioniPrimary (citable) accession number: Q9WTM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.