Q9WTL2 (RAB25_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-25

Gene names

Name:

Rab25

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis By similarity.
Subunit structure	Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cell projection › pseudopodium membrane By similarity. Cytoplasmic vesicle By similarity. Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell projection Cytoplasmic vesicle Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW pseudopodium organization Inferred from sequence or structural similarity. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from sequence or structural similarity. Source: UniProtKB pseudopodium Inferred from sequence or structural similarity. Source: UniProtKB pseudopodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Ras-related protein Rab-25		PRO_0000121216
	Propeptide	211 – 213	3	Removed in mature form Potential		PRO_0000370822
Regions
	Nucleotide binding	19 – 27	9	GTP By similarity
	Nucleotide binding	67 – 71	5	GTP By similarity
	Nucleotide binding	125 – 128	4	GTP By similarity
	Nucleotide binding	155 – 157	3	GTP By similarity
	Motif	41 – 49	9	Effector region By similarity
Amino acid modifications
	Modified residue	210	1	Cysteine methyl ester Potential
	Lipidation	209	1	S-geranylgeranyl cysteine By similarity
	Lipidation	210	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	203	1	P → L in AAD39911. Ref.1
	Sequence conflict	203	1	P → L in AAD39912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9WTL2 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 1359F7B4EA88D070	FASTA	213	23,473
	10 20 30 40 50 60 MGNRTDEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS TRTVMLGTAA 70 80 90 100 110 120 VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY AVVERWLKEL YDHAEATIVV 130 140 150 160 170 180 MLVGNKSDLS QAREVPTEEA CMFAENNGLL FLETSALDST NVELAFQTVL KEIFAKVSKQ 190 200 210 KQNSTRTSAI TLGNAQAGQD PGPGEKRACC ISL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure of murine rab11 family members." Bhartur S.G., Calhoun B.C., Woodrum J., Kurkjian J., Iyer S., Lai F., Goldenring J.R. Biochem. Biophys. Res. Commun. 269:611-617(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 15-25 AND 76-83, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF119675 mRNA. Translation: AAD39911.1. AF119676 Genomic DNA. Translation: AAD39912.1. AK003260 mRNA. Translation: BAB22676.1. BC006624 mRNA. Translation: AAH06624.1.
IPI	IPI00123514.
RefSeq	NP_058595.2. NM_016899.4.
UniGene	Mm.26994.
3D structure databases
ProteinModelPortal	Q9WTL2.
SMR	Q9WTL2. Positions 10-178.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WTL2. 1 interaction.
MINT	MINT-219202.
PTM databases
PhosphoSite	Q9WTL2.
Proteomic databases
PaxDb	Q9WTL2.
PRIDE	Q9WTL2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000008745; ENSMUSP00000008745; ENSMUSG00000008601.
GeneID	53868.
KEGG	mmu:53868.
Organism-specific databases
CTD	57111.
MGI	MGI:1858203. Rab25.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00690000101664.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	Q9WTL2.
KO	K07906.
OMA	RTDEDYN.
OrthoDB	EOG4NS3CK.
Gene expression databases
ArrayExpress	Q9WTL2.
Bgee	Q9WTL2.
CleanEx	MM_RAB25.
Genevestigator	Q9WTL2.
GermOnline	ENSMUSG00000008601. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	310713.
SOURCE	Search...

Entry information

Entry name

RAB25_MOUSE

Accession

Primary (citable) accession number: Q9WTL2
Secondary accession number(s): Q9D1P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program