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Protein

Ras-related protein Rab-25

Gene

Rab25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (PubMed:22696678). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279GTPBy similarity
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi125 – 1284GTPBy similarity
Nucleotide bindingi155 – 1573GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: GO_Central
  2. GTPase activity Source: GO_Central
  3. GTP binding Source: GO_Central
  4. myosin V binding Source: MGI

GO - Biological processi

  1. epithelial cell morphogenesis Source: UniProtKB
  2. exocytosis Source: GO_Central
  3. intracellular protein transport Source: GO_Central
  4. melanosome transport Source: GO_Central
  5. metabolic process Source: GOC
  6. positive regulation of cell proliferation Source: UniProtKB
  7. positive regulation of epithelial cell migration Source: MGI
  8. pseudopodium organization Source: UniProtKB
  9. Rab protein signal transduction Source: GO_Central
  10. regulation of vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-25
Gene namesi
Name:Rab25Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1858203. Rab25.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Cell projectionpseudopodium membrane By similarity. Cytoplasmic vesicle By similarity
Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.By similarity

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. cytoplasmic vesicle Source: UniProtKB
  3. extracellular vesicular exosome Source: MGI
  4. pseudopodium Source: UniProtKB
  5. pseudopodium membrane Source: UniProtKB-SubCell
  6. recycling endosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Ras-related protein Rab-25PRO_0000121216Add
BLAST
Propeptidei211 – 2133Removed in mature formSequence AnalysisPRO_0000370822

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi209 – 2091S-geranylgeranyl cysteineBy similarity
Modified residuei210 – 2101Cysteine methyl esterSequence Analysis
Lipidationi210 – 2101S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ9WTL2.
PaxDbiQ9WTL2.
PRIDEiQ9WTL2.

PTM databases

PhosphoSiteiQ9WTL2.

Expressioni

Gene expression databases

BgeeiQ9WTL2.
CleanExiMM_RAB25.
ExpressionAtlasiQ9WTL2. baseline and differential.
GenevestigatoriQ9WTL2.

Interactioni

Subunit structurei

Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.By similarity

Protein-protein interaction databases

IntActiQ9WTL2. 1 interaction.
MINTiMINT-219202.

Structurei

3D structure databases

ProteinModelPortaliQ9WTL2.
SMRiQ9WTL2. Positions 10-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 499Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9WTL2.
KOiK07906.
OMAiSEDQENG.
OrthoDBiEOG7SFHZ2.
PhylomeDBiQ9WTL2.
TreeFamiTF300099.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WTL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNRTDEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS
60 70 80 90 100
TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY
110 120 130 140 150
AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA CMFAENNGLL
160 170 180 190 200
FLETSALDST NVELAFQTVL KEIFAKVSKQ KQNSTRTSAI TLGNAQAGQD
210
PGPGEKRACC ISL
Length:213
Mass (Da):23,473
Last modified:April 13, 2004 - v2
Checksum:i1359F7B4EA88D070
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031P → L in AAD39911 (PubMed:10708602).Curated
Sequence conflicti203 – 2031P → L in AAD39912 (PubMed:10708602).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119675 mRNA. Translation: AAD39911.1.
AF119676 Genomic DNA. Translation: AAD39912.1.
AK003260 mRNA. Translation: BAB22676.1.
BC006624 mRNA. Translation: AAH06624.1.
CCDSiCCDS17477.1.
RefSeqiNP_058595.2. NM_016899.4.
UniGeneiMm.26994.

Genome annotation databases

EnsembliENSMUST00000008745; ENSMUSP00000008745; ENSMUSG00000008601.
GeneIDi53868.
KEGGimmu:53868.
UCSCiuc008pvn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119675 mRNA. Translation: AAD39911.1.
AF119676 Genomic DNA. Translation: AAD39912.1.
AK003260 mRNA. Translation: BAB22676.1.
BC006624 mRNA. Translation: AAH06624.1.
CCDSiCCDS17477.1.
RefSeqiNP_058595.2. NM_016899.4.
UniGeneiMm.26994.

3D structure databases

ProteinModelPortaliQ9WTL2.
SMRiQ9WTL2. Positions 10-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WTL2. 1 interaction.
MINTiMINT-219202.

PTM databases

PhosphoSiteiQ9WTL2.

Proteomic databases

MaxQBiQ9WTL2.
PaxDbiQ9WTL2.
PRIDEiQ9WTL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008745; ENSMUSP00000008745; ENSMUSG00000008601.
GeneIDi53868.
KEGGimmu:53868.
UCSCiuc008pvn.1. mouse.

Organism-specific databases

CTDi57111.
MGIiMGI:1858203. Rab25.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9WTL2.
KOiK07906.
OMAiSEDQENG.
OrthoDBiEOG7SFHZ2.
PhylomeDBiQ9WTL2.
TreeFamiTF300099.

Miscellaneous databases

NextBioi310713.
PROiQ9WTL2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTL2.
CleanExiMM_RAB25.
ExpressionAtlasiQ9WTL2. baseline and differential.
GenevestigatoriQ9WTL2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Lubec G., Kang S.U.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 15-25 AND 76-83, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Grainyhead-like 2 regulates epithelial morphogenesis by establishing functional tight junctions through the organization of a molecular network among claudin3, claudin4, and Rab25."
    Senga K., Mostov K.E., Mitaka T., Miyajima A., Tanimizu N.
    Mol. Biol. Cell 23:2845-2855(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRAB25_MOUSE
AccessioniPrimary (citable) accession number: Q9WTL2
Secondary accession number(s): Q9D1P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 13, 2004
Last modified: April 1, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.