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Protein

Meiotic recombination protein SPO11

Gene

Spo11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761).2 Publications
Isoform 4: In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138NucleophileBy similarity1
Metal bindingi224MagnesiumBy similarity1
Metal bindingi277MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA catabolic process, endonucleolytic Source: InterPro
  • male meiosis I Source: MGI
  • meiotic DNA double-strand break formation Source: GO_Central
  • meiotic DNA double-strand break processing Source: GO_Central
  • meiotic telomere clustering Source: MGI
  • oogenesis Source: MGI
  • ovarian follicle development Source: MGI
  • protein localization to chromosome Source: MGI
  • reciprocal meiotic recombination Source: MGI
  • spermatid development Source: MGI
  • synapsis Source: MGI
  • synaptonemal complex assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Isomerase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-912446. Meiotic recombination.
R-MMU-912497. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11 (EC:5.99.1.3Curated)
Gene namesi
Name:Spo11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1349669. Spo11.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: MGI
  • nuclear chromosome Source: GO_Central
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454751 – 396Meiotic recombination protein SPO11Add BLAST396

Proteomic databases

PaxDbiQ9WTK8.
PRIDEiQ9WTK8.

PTM databases

iPTMnetiQ9WTK8.
PhosphoSitePlusiQ9WTK8.

Expressioni

Tissue specificityi

High levels are found only in the testis where expression is restricted primarily to meiotic germ cells. Not expressed in spermatogonia. Highest levels are found in pachytene spermatocytes. Very low levels are found in thymus, brain and oocytes of embryonic ovary. Not detected in adult ovary (PubMed:10622720, PubMed:10855504). Isoform 1: Expressed early in meiosis, when most double-strand breaks (DSB) are formed (PubMed:21330546).4 Publications

Developmental stagei

Not detected at day 7 postpartum (dpp). Levels are low at 12 dpp but increase by 17 dpp. High levels are maintained throughout the remainder of testis development.2 Publications

Gene expression databases

BgeeiENSMUSG00000005883.
GenevisibleiQ9WTK8. MM.

Interactioni

Subunit structurei

Isoform 1: Heterotetramer of SPO11 and 2 TOP6BL chains (Probable). Isoform 1: Interacts with TOP6BL (PubMed:26917764). Isoform 4: Does not interact with TOP6BL (PubMed:26917764).Curated1 Publication

Protein-protein interaction databases

DIPiDIP-62006N.
STRINGi10090.ENSMUSP00000059056.

Structurei

3D structure databases

ProteinModelPortaliQ9WTK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
GeneTreeiENSGT00390000001787.
HOGENOMiHOG000225930.
HOVERGENiHBG058605.
InParanoidiQ9WTK8.
KOiK10878.
OMAiWEEQIEL.
OrthoDBiEOG091G0IMI.
PhylomeDBiQ9WTK8.
TreeFamiTF314157.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR004084. Meiosis_Spo11.
IPR013048. Meiotic_Spo11.
IPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF03533. SPO11_like. 1 hit.
PF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01551. SPO11HOMOLOG.
PR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTK8-1) [UniParc]FASTAAdd to basket
Also known as: Spo11beta1 Publication, Beta1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFAPMGPEA SFFDALDRHR ASLLAMVKRG AGETPAGATR VASSSEVLTA
60 70 80 90 100
IENIIQDIIK SLARNEVPAF TIDNRSSWEN IMFDDSVGLR MIPQCTTRKI
110 120 130 140 150
RSDSPKSVKK FALILKVLSM IYKLIQSDTY ATKRDIYYTD SQLFGNQAAV
160 170 180 190 200
DSAIDDISCM LKVPRRSLHV LSTSKGLIAG NLRYMEEDGT RVQCTCSATA
210 220 230 240 250
TAVPTNIQGM QHLITDAKFL LIVEKDATFQ RLLDDNFCSR MSPCIMVTGK
260 270 280 290 300
GVPDLNTRLL VKKLWDTFHI PVFTLVDADP YGIEIMCIYK YGSMSMSFEA
310 320 330 340 350
HNLTIPTIRW LGLLPSDIQR LNIPKDSLIP LTKHDQMKLD SILKRPYITY
360 370 380 390
QPLWKKELEM MADSKMKAEI QALTLLSSDY LSRVYLPNKL RFGGWI
Length:396
Mass (Da):44,570
Last modified:April 23, 2003 - v2
Checksum:iF4B9723C02844A5E
GO
Isoform 2 (identifier: Q9WTK8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-216: Missing.

Show »
Length:392
Mass (Da):44,127
Checksum:iADBD0F00D8A381AB
GO
Isoform 3 (identifier: Q9WTK8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R
     134-134: R → RSNAHSVLTLHLHR

Show »
Length:371
Mass (Da):41,792
Checksum:iE9696495058C76DE
GO
Isoform 4 (identifier: Q9WTK8-4) [UniParc]FASTAAdd to basket
Also known as: Spo11alpha1 Publication, Alpha1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R

Show »
Length:358
Mass (Da):40,325
Checksum:iE400467F9DB0514A
GO
Isoform 5 (identifier: Q9WTK8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R
     213-216: Missing.

Show »
Length:354
Mass (Da):39,883
Checksum:iA2891414C41A86C4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00719744 – 81Missing in isoform 3, isoform 4 and isoform 5. 3 PublicationsAdd BLAST38
Alternative sequenceiVSP_00719882M → R in isoform 3, isoform 4 and isoform 5. 3 Publications1
Alternative sequenceiVSP_007199134R → RSNAHSVLTLHLHR in isoform 3. 2 Publications1
Alternative sequenceiVSP_007200213 – 216Missing in isoform 2 and isoform 5. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126400 mRNA. Translation: AAD49561.1.
AF149309 mRNA. Translation: AAD44811.1.
AF169386 mRNA. Translation: AAD52563.1.
AF163053 mRNA. Translation: AAD43588.1.
AF163052, AF163049, AF163051 Genomic DNA. Translation: AAD43591.1.
AF163052, AF163049, AF163051 Genomic DNA. Translation: AAD43592.1.
AF163054 mRNA. Translation: AAD43589.1.
AF165313 mRNA. Translation: AAF87090.1.
AF167439 mRNA. Translation: AAF87094.1.
AF173848 mRNA. Translation: AAF87096.1.
AF443910 Genomic DNA. Translation: AAL32063.1.
CCDSiCCDS17137.1. [Q9WTK8-1]
CCDS50811.1. [Q9WTK8-3]
CCDS50812.1. [Q9WTK8-4]
RefSeqiNP_001077428.1. NM_001083959.1. [Q9WTK8-3]
NP_001077429.1. NM_001083960.1. [Q9WTK8-4]
NP_001292363.1. NM_001305434.1. [Q9WTK8-5]
NP_036176.1. NM_012046.2. [Q9WTK8-1]
UniGeneiMm.23495.

Genome annotation databases

EnsembliENSMUST00000050442; ENSMUSP00000059056; ENSMUSG00000005883. [Q9WTK8-1]
ENSMUST00000109125; ENSMUSP00000104753; ENSMUSG00000005883. [Q9WTK8-4]
ENSMUST00000109126; ENSMUSP00000104754; ENSMUSG00000005883. [Q9WTK8-3]
GeneIDi26972.
KEGGimmu:26972.
UCSCiuc008odd.1. mouse. [Q9WTK8-1]
uc008ode.1. mouse. [Q9WTK8-4]
uc008odf.1. mouse. [Q9WTK8-3]
uc012ckq.1. mouse. [Q9WTK8-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126400 mRNA. Translation: AAD49561.1.
AF149309 mRNA. Translation: AAD44811.1.
AF169386 mRNA. Translation: AAD52563.1.
AF163053 mRNA. Translation: AAD43588.1.
AF163052, AF163049, AF163051 Genomic DNA. Translation: AAD43591.1.
AF163052, AF163049, AF163051 Genomic DNA. Translation: AAD43592.1.
AF163054 mRNA. Translation: AAD43589.1.
AF165313 mRNA. Translation: AAF87090.1.
AF167439 mRNA. Translation: AAF87094.1.
AF173848 mRNA. Translation: AAF87096.1.
AF443910 Genomic DNA. Translation: AAL32063.1.
CCDSiCCDS17137.1. [Q9WTK8-1]
CCDS50811.1. [Q9WTK8-3]
CCDS50812.1. [Q9WTK8-4]
RefSeqiNP_001077428.1. NM_001083959.1. [Q9WTK8-3]
NP_001077429.1. NM_001083960.1. [Q9WTK8-4]
NP_001292363.1. NM_001305434.1. [Q9WTK8-5]
NP_036176.1. NM_012046.2. [Q9WTK8-1]
UniGeneiMm.23495.

3D structure databases

ProteinModelPortaliQ9WTK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-62006N.
STRINGi10090.ENSMUSP00000059056.

PTM databases

iPTMnetiQ9WTK8.
PhosphoSitePlusiQ9WTK8.

Proteomic databases

PaxDbiQ9WTK8.
PRIDEiQ9WTK8.

Protocols and materials databases

DNASUi26972.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050442; ENSMUSP00000059056; ENSMUSG00000005883. [Q9WTK8-1]
ENSMUST00000109125; ENSMUSP00000104753; ENSMUSG00000005883. [Q9WTK8-4]
ENSMUST00000109126; ENSMUSP00000104754; ENSMUSG00000005883. [Q9WTK8-3]
GeneIDi26972.
KEGGimmu:26972.
UCSCiuc008odd.1. mouse. [Q9WTK8-1]
uc008ode.1. mouse. [Q9WTK8-4]
uc008odf.1. mouse. [Q9WTK8-3]
uc012ckq.1. mouse. [Q9WTK8-5]

Organism-specific databases

CTDi23626.
MGIiMGI:1349669. Spo11.

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
GeneTreeiENSGT00390000001787.
HOGENOMiHOG000225930.
HOVERGENiHBG058605.
InParanoidiQ9WTK8.
KOiK10878.
OMAiWEEQIEL.
OrthoDBiEOG091G0IMI.
PhylomeDBiQ9WTK8.
TreeFamiTF314157.

Enzyme and pathway databases

ReactomeiR-MMU-912446. Meiotic recombination.
R-MMU-912497. Meiotic Recombination.

Miscellaneous databases

PROiQ9WTK8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005883.
GenevisibleiQ9WTK8. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR004084. Meiosis_Spo11.
IPR013048. Meiotic_Spo11.
IPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF03533. SPO11_like. 1 hit.
PF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01551. SPO11HOMOLOG.
PR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPO11_MOUSE
AccessioniPrimary (citable) accession number: Q9WTK8
Secondary accession number(s): Q8VIH6
, Q9QUK2, Q9QY57, Q9QZS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 23, 2003
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.