Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Meiotic recombination protein SPO11

Gene

Spo11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform 1: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761).2 Publications
Isoform 4: In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138NucleophileBy similarity1
Metal bindingi224MagnesiumBy similarity1
Metal bindingi277MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA catabolic process, endonucleolytic Source: InterPro
  • double-strand break repair involved in meiotic recombination Source: MGI
  • male meiosis I Source: MGI
  • meiotic DNA double-strand break formation Source: GO_Central
  • meiotic DNA double-strand break processing Source: GO_Central
  • meiotic telomere clustering Source: MGI
  • oogenesis Source: MGI
  • ovarian follicle development Source: MGI
  • protein localization to chromosome Source: MGI
  • reciprocal meiotic recombination Source: MGI
  • spermatid development Source: MGI
  • synapsis Source: MGI
  • synaptonemal complex assembly Source: MGI

Keywordsi

Molecular functionDNA-binding, Hydrolase, Isomerase
Biological processMeiosis
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11 (EC:5.99.1.3Curated)
Gene namesi
Name:Spo11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1349669 Spo11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454751 – 396Meiotic recombination protein SPO11Add BLAST396

Proteomic databases

PaxDbiQ9WTK8
PRIDEiQ9WTK8

PTM databases

iPTMnetiQ9WTK8
PhosphoSitePlusiQ9WTK8

Expressioni

Tissue specificityi

High levels are found only in the testis where expression is restricted primarily to meiotic germ cells. Not expressed in spermatogonia. Highest levels are found in pachytene spermatocytes. Very low levels are found in thymus, brain and oocytes of embryonic ovary. Not detected in adult ovary (PubMed:10622720, PubMed:10855504). Isoform 1: Expressed early in meiosis, when most double-strand breaks (DSB) are formed (PubMed:21330546).4 Publications

Developmental stagei

Not detected at day 7 postpartum (dpp). Levels are low at 12 dpp but increase by 17 dpp. High levels are maintained throughout the remainder of testis development.2 Publications

Gene expression databases

BgeeiENSMUSG00000005883
GenevisibleiQ9WTK8 MM

Interactioni

Subunit structurei

Isoform 1: Heterotetramer of SPO11 and 2 TOP6BL chains (Probable). Isoform 1: Interacts with TOP6BL (PubMed:26917764). Isoform 4: Does not interact with TOP6BL (PubMed:26917764).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Top6blJ3QMY96EBI-16201014,EBI-16200997

Protein-protein interaction databases

BioGridi205092, 1 interactor
DIPiDIP-62006N
IntActiQ9WTK8, 1 interactor
STRINGi10090.ENSMUSP00000059056

Structurei

3D structure databases

ProteinModelPortaliQ9WTK8
SMRiQ9WTK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795 Eukaryota
COG1697 LUCA
GeneTreeiENSGT00390000001787
HOGENOMiHOG000225930
HOVERGENiHBG058605
InParanoidiQ9WTK8
KOiK10878
OMAiFTDGDPY
OrthoDBiEOG091G0IMI
PhylomeDBiQ9WTK8
TreeFamiTF314157

Family and domain databases

CDDicd00223 TOPRIM_TopoIIB_SPO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR004084 Meiosis_Spo11
IPR013048 Meiotic_Spo11
IPR002815 Spo11/TopoVI_A
IPR013049 Spo11/TopoVI_A_N
IPR036078 Spo11/TopoVI_A_sf
IPR034136 TOPRIM_Topo6A/Spo11
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10848 PTHR10848, 1 hit
PfamiView protein in Pfam
PF03533 SPO11_like, 1 hit
PF04406 TP6A_N, 1 hit
PRINTSiPR01551 SPO11HOMOLOG
PR01550 TOP6AFAMILY
SUPFAMiSSF56726 SSF56726, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTK8-1) [UniParc]FASTAAdd to basket
Also known as: Spo11beta1 Publication, Beta1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFAPMGPEA SFFDALDRHR ASLLAMVKRG AGETPAGATR VASSSEVLTA
60 70 80 90 100
IENIIQDIIK SLARNEVPAF TIDNRSSWEN IMFDDSVGLR MIPQCTTRKI
110 120 130 140 150
RSDSPKSVKK FALILKVLSM IYKLIQSDTY ATKRDIYYTD SQLFGNQAAV
160 170 180 190 200
DSAIDDISCM LKVPRRSLHV LSTSKGLIAG NLRYMEEDGT RVQCTCSATA
210 220 230 240 250
TAVPTNIQGM QHLITDAKFL LIVEKDATFQ RLLDDNFCSR MSPCIMVTGK
260 270 280 290 300
GVPDLNTRLL VKKLWDTFHI PVFTLVDADP YGIEIMCIYK YGSMSMSFEA
310 320 330 340 350
HNLTIPTIRW LGLLPSDIQR LNIPKDSLIP LTKHDQMKLD SILKRPYITY
360 370 380 390
QPLWKKELEM MADSKMKAEI QALTLLSSDY LSRVYLPNKL RFGGWI
Length:396
Mass (Da):44,570
Last modified:April 23, 2003 - v2
Checksum:iF4B9723C02844A5E
GO
Isoform 2 (identifier: Q9WTK8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-216: Missing.

Show »
Length:392
Mass (Da):44,127
Checksum:iADBD0F00D8A381AB
GO
Isoform 3 (identifier: Q9WTK8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R
     134-134: R → RSNAHSVLTLHLHR

Show »
Length:371
Mass (Da):41,792
Checksum:iE9696495058C76DE
GO
Isoform 4 (identifier: Q9WTK8-4) [UniParc]FASTAAdd to basket
Also known as: Spo11alpha1 Publication, Alpha1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R

Show »
Length:358
Mass (Da):40,325
Checksum:iE400467F9DB0514A
GO
Isoform 5 (identifier: Q9WTK8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-81: Missing.
     82-82: M → R
     213-216: Missing.

Show »
Length:354
Mass (Da):39,883
Checksum:iA2891414C41A86C4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00719744 – 81Missing in isoform 3, isoform 4 and isoform 5. 3 PublicationsAdd BLAST38
Alternative sequenceiVSP_00719882M → R in isoform 3, isoform 4 and isoform 5. 3 Publications1
Alternative sequenceiVSP_007199134R → RSNAHSVLTLHLHR in isoform 3. 2 Publications1
Alternative sequenceiVSP_007200213 – 216Missing in isoform 2 and isoform 5. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126400 mRNA Translation: AAD49561.1
AF149309 mRNA Translation: AAD44811.1
AF169386 mRNA Translation: AAD52563.1
AF163053 mRNA Translation: AAD43588.1
AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43591.1
AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43592.1
AF163054 mRNA Translation: AAD43589.1
AF165313 mRNA Translation: AAF87090.1
AF167439 mRNA Translation: AAF87094.1
AF173848 mRNA Translation: AAF87096.1
AF443910 Genomic DNA Translation: AAL32063.1
CCDSiCCDS17137.1 [Q9WTK8-1]
CCDS50811.1 [Q9WTK8-3]
CCDS50812.1 [Q9WTK8-4]
RefSeqiNP_001077428.1, NM_001083959.1 [Q9WTK8-3]
NP_001077429.1, NM_001083960.1 [Q9WTK8-4]
NP_001292363.1, NM_001305434.1 [Q9WTK8-5]
NP_036176.1, NM_012046.2 [Q9WTK8-1]
UniGeneiMm.23495

Genome annotation databases

EnsembliENSMUST00000050442; ENSMUSP00000059056; ENSMUSG00000005883 [Q9WTK8-1]
ENSMUST00000109125; ENSMUSP00000104753; ENSMUSG00000005883 [Q9WTK8-4]
ENSMUST00000109126; ENSMUSP00000104754; ENSMUSG00000005883 [Q9WTK8-3]
GeneIDi26972
KEGGimmu:26972
UCSCiuc008odd.1 mouse [Q9WTK8-1]
uc008ode.1 mouse [Q9WTK8-4]
uc008odf.1 mouse [Q9WTK8-3]
uc012ckq.1 mouse [Q9WTK8-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPO11_MOUSE
AccessioniPrimary (citable) accession number: Q9WTK8
Secondary accession number(s): Q8VIH6
, Q9QUK2, Q9QY57, Q9QZS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 23, 2003
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health