Q9WTK2 (CDYL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromodomain Y-like protein
Short name=CDY-like
EC=2.3.1.48

Gene names

Name:

Cdyl

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has histone acetyltransferase activity. May play a role in histone hyperacetylation during spermatid maturation. Acts as repressor of transcription. Ref.4 Ref.5
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	Interacts with HDAC1 and HDAC2 via its C-terminal acetyl-CoA-binding domain. Ref.5
Subcellular location	Nucleus Ref.4 Ref.5.
Tissue specificity	Highly expressed in testis (at protein level). Expressed as 2 transcripts: a ubiquitous transcript and a highly expressed testis-specific transcript.
Developmental stage	Highly expressed in elongating spermatids during histone hyperacetylation. Ref.4 Ref.5
Miscellaneous	Interaction with HDAC1 or HDAC2 prevents coenzyme A binding.
Sequence similarities	Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Acyltransferase Repressor Transferase
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone acetylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9WTK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9WTK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     50-57: AIQDAETQ → MASEELYE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Chromodomain Y-like protein		PRO_0000080222
Regions
	Domain	56 – 116	61	Chromo
Amino acid modifications
	Modified residue	83	1	Phosphoserine By similarity
	Modified residue	130	1	N6,N6,N6-trimethyllysine; by EHMT2; alternate By similarity
	Modified residue	130	1	N6,N6-dimethyllysine; by EHMT2; alternate By similarity
	Modified residue	130	1	N6-methyllysine; by EHMT2; alternate By similarity
	Modified residue	187	1	Phosphoserine Ref.6
	Modified residue	196	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 49	49	Missing in isoform 2.		VSP_026385
	Alternative sequence	50 – 57	8	AIQDAETQ → MASEELYE in isoform 2.		VSP_026386
Experimental info
	Mutagenesis	516	1	S → A: Abolishes CoA-binding. No effect on transcriptional repressor activity. Ref.5
	Mutagenesis	588 – 589	2	RK → AA: Abolishes CoA-binding. No effect on transcriptional repressor activity.
	Sequence conflict	489	1	M → I in BAE33739. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 470D5B97D7E52CCA	FASTA	593	65,211
	10 20 30 40 50 60 MGIGNSQPNS QEAQLCTLPE KAEQPTDDNT CQQNNVVPAT VSEPDQASPA IQDAETQVES 70 80 90 100 110 120 IVDKRKNKKG KTEYLVRWKG YDSEDDTWEP EQHLVNCEEY IHDFNRRHNE RQKEGSLARA 130 140 150 160 170 180 SRASPSNARK QISRSTHSTL SKTNSKALVV GKDHESKSSQ LLAASQKFRK NPAPSLANRK 190 200 210 220 230 240 NMDLAKSGIK ILVPKSPVKG RTSVDGFQGE SPEKLDPVDQ GAEDTVAPEV TAEKPTGALL 250 260 270 280 290 300 GPGAERARMG SRPRIHPLVP QVSGPVTAAM ATGLAVNGKG TSPFMDALAA NGTVTIQTSV 310 320 330 340 350 360 TGVTAGKRKF IDDRRDQPFD KRLRFSVRQT ESAYRYRDIV VRKQDGFTHI LLSTKSSENN 370 380 390 400 410 420 SLNPEVMKEV QSALSTAAAD DSKLVLLSAV GSVFCCGLDF IYFIRRLTDD RKRESTKMAD 430 440 450 460 470 480 AIRNFVNTFI QFKKPIIVAV NGPAIGLGAS ILPLCDVVWA NEKAWFQTPY TTFGQSPDGC 490 500 510 520 530 540 STVMFPKIMG GASANEMLFS GRKLTAQEAC GKGLVSQVFW PGTFTQEVMV RIKELASCNP 550 560 570 580 590 VVLEESKALV RCNMKMELEQ ANERECEVLK KIWGSAQGMD SMLKYLQRKI DEF
	Isoform 2 [UniParc]. Checksum: B8E3B85969ED9103	FASTA	544	60,142
	10 20 30 40 50 60 MASEELYEVE SIVDKRKNKK GKTEYLVRWK GYDSEDDTWE PEQHLVNCEE YIHDFNRRHN 70 80 90 100 110 120 ERQKEGSLAR ASRASPSNAR KQISRSTHST LSKTNSKALV VGKDHESKSS QLLAASQKFR 130 140 150 160 170 180 KNPAPSLANR KNMDLAKSGI KILVPKSPVK GRTSVDGFQG ESPEKLDPVD QGAEDTVAPE 190 200 210 220 230 240 VTAEKPTGAL LGPGAERARM GSRPRIHPLV PQVSGPVTAA MATGLAVNGK GTSPFMDALA 250 260 270 280 290 300 ANGTVTIQTS VTGVTAGKRK FIDDRRDQPF DKRLRFSVRQ TESAYRYRDI VVRKQDGFTH 310 320 330 340 350 360 ILLSTKSSEN NSLNPEVMKE VQSALSTAAA DDSKLVLLSA VGSVFCCGLD FIYFIRRLTD 370 380 390 400 410 420 DRKRESTKMA DAIRNFVNTF IQFKKPIIVA VNGPAIGLGA SILPLCDVVW ANEKAWFQTP 430 440 450 460 470 480 YTTFGQSPDG CSTVMFPKIM GGASANEMLF SGRKLTAQEA CGKGLVSQVF WPGTFTQEVM 490 500 510 520 530 540 VRIKELASCN PVVLEESKAL VRCNMKMELE QANERECEVL KKIWGSAQGM DSMLKYLQRK IDEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome." Lahn B.T., Page D.C. Nat. Genet. 21:429-433(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: NOD. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[4]	"Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis." Lahn B.T., Tang Z.L., Zhou J., Barndt R.J., Parvinen M., Allis C.D., Page D.C. Proc. Natl. Acad. Sci. U.S.A. 99:8707-8712(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, ALTERNATIVE SPLICING.
[5]	"Cdyl: a new transcriptional co-repressor." Caron C., Pivot-Pajot C., van Grunsven L.A., Col E., Lestrat C., Rousseaux S., Khochbin S. EMBO Rep. 4:877-882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH HDAC1 AND HDAC2, MUTAGENESIS OF SER-516 AND 588-ARG-LYS-589.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187 AND SER-196, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081260 mRNA. Translation: AAD22736.1. AF081261 mRNA. Translation: AAD22737.1. AK156509 mRNA. Translation: BAE33739.1. BC055103 mRNA. Translation: AAH55103.1. BC062123 mRNA. Translation: AAH62123.1.
IPI	IPI00466664. IPI00849838.
RefSeq	NP_001116858.1. NM_001123386.1. NP_034011.1. NM_009881.3.
UniGene	Mm.29002.
3D structure databases
ProteinModelPortal	Q9WTK2.
SMR	Q9WTK2. Positions 57-114, 336-593.
ModBase	Search...
PTM databases
PhosphoSite	Q9WTK2.
Proteomic databases
PaxDb	Q9WTK2.
PRIDE	Q9WTK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075220; ENSMUSP00000074707; ENSMUSG00000059288. ENSMUST00000163595; ENSMUSP00000131784; ENSMUSG00000059288.
GeneID	12593.
KEGG	mmu:12593.
UCSC	uc007qce.2. mouse. uc007qcf.2. mouse.
Organism-specific databases
CTD	9425.
MGI	MGI:1339956. Cdyl.
Phylogenomic databases
eggNOG	COG1024.
GeneTree	ENSGT00670000097595.
HOGENOM	HOG000111507.
HOVERGEN	HBG006723.
InParanoid	Q9WTK2.
OMA	LVRCNMK.
OrthoDB	EOG4KPT9K.
Gene expression databases
ArrayExpress	Q9WTK2.
Bgee	Q9WTK2.
CleanEx	MM_CDYL.
Genevestigator	Q9WTK2.
GermOnline	ENSMUSG00000059288. Mus musculus.
Family and domain databases
InterPro	IPR023780. Chromo_domain. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR023779. Chromodomain_CS. IPR001753. Crotonase_core_superfam. [Graphical view]
Pfam	PF00385. Chromo. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. [Graphical view]
SUPFAM	SSF54160. Chromodomain-like. 1 hit.
PROSITE	PS00598. CHROMO_1. 1 hit. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281746.
SOURCE	Search...

Entry information

Entry name

CDYL_MOUSE

Accession

Primary (citable) accession number: Q9WTK2
Secondary accession number(s): Q3U0W2, Q6P6N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program