Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tesmin

Gene

Mtl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in spermatogenesis.Curated

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Tesmin
Alternative name(s):
Metallothionein-like 5, testis-specific
Testis-specific metallothionein-like protein
Gene namesi
Name:Mtl5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1340029. Mtl5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475TesminPRO_0000096627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341PhosphoserineCombined sources
Modified residuei67 – 671PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WTJ6.
PRIDEiQ9WTJ6.

PTM databases

iPTMnetiQ9WTJ6.
PhosphoSiteiQ9WTJ6.

Expressioni

Tissue specificityi

Expressed in spermatocytes and adult heart. Also expressed in fetal brain, heart, kidney and ovary.3 Publications

Developmental stagei

Expressed as early as day 8, coincident with the entry of germ cells into meiosis. Expression then progressively increases. Expressed in the pachytene stage.2 Publications

Gene expression databases

BgeeiQ9WTJ6.
CleanExiMM_MTL5.
ExpressionAtlasiQ9WTJ6. baseline and differential.
GenevisibleiQ9WTJ6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025840.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini263 – 372110CRCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lin-54 family.Curated
Contains 1 CRC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1171. Eukaryota.
ENOG4110UR5. LUCA.
GeneTreeiENSGT00390000013974.
HOGENOMiHOG000082453.
HOVERGENiHBG108153.
InParanoidiQ9WTJ6.
OMAiHHLSPTK.
OrthoDBiEOG7DFXBK.
PhylomeDBiQ9WTJ6.
TreeFamiTF313189.

Family and domain databases

InterProiIPR005172. CRC.
IPR028307. Lin-54_fam.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR12446. PTHR12446. 1 hit.
PfamiPF03638. TCR. 1 hit.
[Graphical view]
SMARTiSM01114. CXC. 1 hit.
[Graphical view]
PROSITEiPS51634. CRC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WTJ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDALLGAMT GPEDELGAEL FGSERVFADG LALSPAGGAA DRDELPVLAD
60 70 80 90 100
AYLGATEPGE PLLRALSPPP GAEVPAALLG DFPGLPELRS PDDAAPPPAY
110 120 130 140 150
SVHVLSSLLP GARGPALLPL SAGVRVIPVE IKEAGGSVPG GSPEDAAFQA
160 170 180 190 200
PLAQESCCKF PSSQEAEEAS SCPRKKDSSP MVICQLKGGA QMLCIDNCGA
210 220 230 240 250
RELKALHLLP QYDDQSSFPQ SELPKPMTTL VGRLLPVPAK LNLITQVDNG
260 270 280 290 300
ALPSAVNGAA FPSGPALQGP PKITLSGYCD CFSSGDFCNS CSCNNLRHEL
310 320 330 340 350
ERFKAIKACL DRNPEAFQPK MGKGRLGAAK LRHSKGCNCK RSGCLKNYCE
360 370 380 390 400
CYEAKIMCSS ICKCIACKNY EESPERKMLM STPHYMEPGD FESSHYLSPA
410 420 430 440 450
KFSGPPKLRK NRQAFSCISW EVVEATCACL LAQGEEAEQE HCSPSLAEQM
460 470
ILEEFGRCLS QILHIEFKSK GLKIE
Length:475
Mass (Da):50,616
Last modified:September 2, 2008 - v2
Checksum:i4373ED1188DCF79F
GO
Isoform 2 (identifier: Q9WTJ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:295
Mass (Da):32,488
Checksum:i516B9E1E1778251C
GO

Sequence cautioni

The sequence AAD24667.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331Q → H in BAB64934 (PubMed:12606435).Curated
Sequence conflicti439 – 4391Q → H in BAB64934 (PubMed:12606435).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 180180Missing in isoform 2. 2 PublicationsVSP_035300Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67176 mRNA. Translation: AAD24666.1.
U77383 mRNA. Translation: AAD24667.1. Different initiation.
AB057423 mRNA. Translation: BAB64935.1.
AB057422 Genomic DNA. Translation: BAB64934.1.
AF329359 mRNA. Translation: AAM54491.1.
AF329360 mRNA. Translation: AAM54492.1.
BC024377 mRNA. Translation: AAH24377.1.
CCDSiCCDS37880.1. [Q9WTJ6-1]
CCDS70912.1. [Q9WTJ6-2]
RefSeqiNP_001034746.1. NM_001039657.2. [Q9WTJ6-1]
NP_001273486.1. NM_001286557.1. [Q9WTJ6-2]
NP_001273487.1. NM_001286558.1. [Q9WTJ6-2]
UniGeneiMm.209941.

Genome annotation databases

EnsembliENSMUST00000025840; ENSMUSP00000025840; ENSMUSG00000024905. [Q9WTJ6-1]
ENSMUST00000127142; ENSMUSP00000122687; ENSMUSG00000024905. [Q9WTJ6-2]
ENSMUST00000142193; ENSMUSP00000114171; ENSMUSG00000024905. [Q9WTJ6-2]
GeneIDi17771.
KEGGimmu:17771.
UCSCiuc008fwg.2. mouse. [Q9WTJ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67176 mRNA. Translation: AAD24666.1.
U77383 mRNA. Translation: AAD24667.1. Different initiation.
AB057423 mRNA. Translation: BAB64935.1.
AB057422 Genomic DNA. Translation: BAB64934.1.
AF329359 mRNA. Translation: AAM54491.1.
AF329360 mRNA. Translation: AAM54492.1.
BC024377 mRNA. Translation: AAH24377.1.
CCDSiCCDS37880.1. [Q9WTJ6-1]
CCDS70912.1. [Q9WTJ6-2]
RefSeqiNP_001034746.1. NM_001039657.2. [Q9WTJ6-1]
NP_001273486.1. NM_001286557.1. [Q9WTJ6-2]
NP_001273487.1. NM_001286558.1. [Q9WTJ6-2]
UniGeneiMm.209941.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025840.

PTM databases

iPTMnetiQ9WTJ6.
PhosphoSiteiQ9WTJ6.

Proteomic databases

PaxDbiQ9WTJ6.
PRIDEiQ9WTJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025840; ENSMUSP00000025840; ENSMUSG00000024905. [Q9WTJ6-1]
ENSMUST00000127142; ENSMUSP00000122687; ENSMUSG00000024905. [Q9WTJ6-2]
ENSMUST00000142193; ENSMUSP00000114171; ENSMUSG00000024905. [Q9WTJ6-2]
GeneIDi17771.
KEGGimmu:17771.
UCSCiuc008fwg.2. mouse. [Q9WTJ6-1]

Organism-specific databases

CTDi9633.
MGIiMGI:1340029. Mtl5.

Phylogenomic databases

eggNOGiKOG1171. Eukaryota.
ENOG4110UR5. LUCA.
GeneTreeiENSGT00390000013974.
HOGENOMiHOG000082453.
HOVERGENiHBG108153.
InParanoidiQ9WTJ6.
OMAiHHLSPTK.
OrthoDBiEOG7DFXBK.
PhylomeDBiQ9WTJ6.
TreeFamiTF313189.

Miscellaneous databases

NextBioi292485.
PROiQ9WTJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WTJ6.
CleanExiMM_MTL5.
ExpressionAtlasiQ9WTJ6. baseline and differential.
GenevisibleiQ9WTJ6. MM.

Family and domain databases

InterProiIPR005172. CRC.
IPR028307. Lin-54_fam.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR12446. PTHR12446. 1 hit.
PfamiPF03638. TCR. 1 hit.
[Graphical view]
SMARTiSM01114. CXC. 1 hit.
[Graphical view]
PROSITEiPS51634. CRC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation."
    Sugihara T., Wadhwa R., Kaul S.C., Mitsui Y.
    Genomics 57:130-136(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 91-475 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: ICR.
    Tissue: Testis.
  2. "Germ cell-specific nucleocytoplasmic shuttling protein, tesmin, responsive to heavy metal stress in mouse testes."
    Matsuura T., Kawasaki Y., Miwa K., Sutou S., Ohinata Y., Yoshida F., Mitsui Y.
    J. Inorg. Biochem. 88:183-191(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: BALB/cJ.
  3. "Native tesmin is a 60-kilodalton protein that undergoes dynamic changes in its localization during spermatogenesis in mice."
    Sutou S., Miwa K., Matsuura T., Kawasaki Y., Ohinata Y., Mitsui Y.
    Biol. Reprod. 68:1861-1869(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: 129.
  4. "Tesmin transcription is regulated differently during male and female meiosis."
    Olesen C., Moeller M., Byskov A.G.
    Mol. Reprod. Dev. 67:116-126(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Strain: C57BL/6 X DBA/2.
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiMTL5_MOUSE
AccessioniPrimary (citable) accession number: Q9WTJ6
Secondary accession number(s): Q548K4, Q8VIE0, Q8VIE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 2, 2008
Last modified: May 11, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.