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Q9WJB2 (RPOA_PRRSR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 16 chains:

  1. Nsp1
    EC=3.4.22.-
  2. Nsp1-alpha papain-like cysteine proteinase
    EC=3.4.22.-
    Alternative name(s):
    PCP1-alpha
  3. Nsp1-beta papain-like cysteine proteinase
    EC=3.4.22.-
    Alternative name(s):
    PCP1-beta
  4. Nsp2 cysteine proteinase
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    CP2
    Short name=CP
  5. Non-structural protein 3
    Short name=Nsp3
  6. 3C-like serine proteinase
    Short name=3CLSP
    EC=3.4.21.-
    Alternative name(s):
    Nsp4
  7. Non-structural protein 5-6-7
    Short name=Nsp5-6-7
  8. Non-structural protein 5
    Short name=Nsp5
  9. Non-structural protein 6
    Short name=Nsp6
  10. Non-structural protein 7-alpha
    Short name=Nsp7-alpha
  11. Non-structural protein 7-beta
    Short name=Nsp7-beta
  12. Non-structural protein 8
    Short name=Nsp8
  13. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    Nsp9
  14. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    Nsp10
  15. Non-structural protein 11
    Short name=Nsp11
  16. Non-structural protein 12
    Short name=Nsp12
Gene names
Name:rep
ORF Names:1a-1b
OrganismPorcine reproductive and respiratory syndrome virus (strain VR-2332) (PRRSV)
Taxonomic identifier300559 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesArteriviridaeArterivirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length3960 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. Ref.4 Ref.5 Ref.6 Ref.7

Nsp1 is essential for viral subgenomic mRNA synthesis By similarity. Ref.4 Ref.5 Ref.6 Ref.7

Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways. Ref.4 Ref.5 Ref.6 Ref.7

Nsp1-beta seems to inhibit the translocation of STAT1 into the cell nucleus. Ref.4 Ref.5 Ref.6 Ref.7

Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation By similarity. Ref.4 Ref.5 Ref.6 Ref.7

The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein By similarity. Ref.4 Ref.5 Ref.6 Ref.7

The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity By similarity. Ref.4 Ref.5 Ref.6 Ref.7

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nsp1: Host nucleus. Host cytoplasm Ref.5.

Nsp1-alpha papain-like cysteine proteinase: Host nucleus. Host cytoplasm Ref.5.

Nsp1-beta papain-like cysteine proteinase: Host cytoplasm Ref.5.

Nsp2 cysteine proteinase: Host membrane; Multi-pass membrane protein Potential Ref.5.

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential Ref.5.

Non-structural protein 5-6-7: Host membrane; Multi-pass membrane protein Potential Ref.5.

3C-like serine proteinase: Host cytoplasm Potential Ref.5.

RNA-directed RNA polymerase: Host cytoplasmhost perinuclear region Potential Ref.5.

Helicase: Host cytoplasmhost perinuclear region Potential Ref.5.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

The OTU-like region is responsible for the deubiquitinating and deISGylation activities of Nsp2 By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. Nsp1 is autocleaved into two subunits, Nsp1-alpha and Nsp1-beta. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4 By similarity. Ref.6

Sequence similarities

Belongs to the arteriviridae polyprotein family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 AV MBD (arterivirus metal-binding) domain.

Contains 1 peptidase C31 domain.

Contains 1 peptidase C32 domain.

Contains 1 peptidase C33 domain.

Contains 1 peptidase S32 domain.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAD12125.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAO13192.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processHost-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Inhibition of host NF-kappa-B by virus
Inhibition of host STAT1 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Host nucleus
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainTransmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionHelicase
Hydrolase
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Serine protease
Thiol protease
Transferase
   Technical termDirect protein sequencing
Multifunctional enzyme
Gene Ontology (GO)
   Biological_processmodulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host NF-kappaB transcription factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host STAT1 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: Q9WJB2-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: Q9WJB2-2)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform differs from the canonical sequence as follows:
     2504-3960: Missing.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 39603960Replicase polyprotein 1ab
PRO_0000036696
Chain1 – 382382Nsp1 By similarity
PRO_0000410829
Chain1 – 180180Nsp1-alpha papain-like cysteine proteinase
PRO_0000036698
Chain181 – 383203Nsp1-beta papain-like cysteine proteinase
PRO_0000036699
Chain384 – 15791196Nsp2 cysteine proteinase Potential
PRO_0000036700
Chain1580 – 1809230Non-structural protein 3 By similarity
PRO_0000036701
Chain1810 – 20132043C-like serine proteinase By similarity
PRO_0000036702
Chain2014 – 2458445Non-structural protein 5-6-7 By similarity
PRO_0000036703
Chain2014 – 2183170Non-structural protein 5 By similarity
PRO_0000423130
Chain2184 – 219916Non-structural protein 6 By similarity
PRO_0000423131
Chain2200 – 2348149Non-structural protein 7-alpha By similarity
PRO_0000423132
Chain2349 – 2458110Non-structural protein 7-beta By similarity
PRO_0000423133
Chain2459 – 3143685RNA-directed RNA polymerase By similarity
PRO_0000036704
Chain2459 – 250345Non-structural protein 8 By similarity
PRO_0000036705
Chain3144 – 3584441Helicase By similarity
PRO_0000036706
Chain3585 – 3807223Non-structural protein 11 By similarity
PRO_0000036707
Chain3808 – 3960153Non-structural protein 12 By similarity
PRO_0000036708

Regions

Transmembrane1266 – 128621Helical; Potential
Transmembrane1296 – 131621Helical; Potential
Transmembrane1345 – 136521Helical; Potential
Transmembrane1368 – 138821Helical; Potential
Transmembrane1583 – 160321Helical; Potential
Transmembrane1650 – 167021Helical; Potential
Transmembrane1685 – 170521Helical; Potential
Transmembrane1719 – 173921Helical; Potential
Transmembrane2012 – 203221Helical; Potential
Transmembrane2060 – 208021Helical; Potential
Transmembrane2092 – 211221Helical; Potential
Transmembrane2137 – 215721Helical; Potential
Transmembrane2164 – 218421Helical; Potential
Domain69 – 180112Peptidase C31
Domain263 – 383121Peptidase C32
Domain428 – 535108Peptidase C33
Domain1810 – 2013204Peptidase S32
Domain2889 – 3023135RdRp catalytic
Domain3144 – 320764AV MBD
Domain3264 – 3416153(+)RNA virus helicase ATP-binding
Domain3417 – 3545129(+)RNA virus helicase C-terminal
Zinc finger8 – 2821C4-type; atypical
Nucleotide binding3292 – 32998ATP By similarity
Region69 – 182114PCP1-alpha
Region263 – 382120PCP1-beta
Region426 – 51388OTU-like
Region1266 – 1388123HD1
Region1583 – 1745163HD2
Region2036 – 2157122HD3
Compositional bias808 – 930123Pro-rich
Compositional bias2329 – 234416Pro-rich

Sites

Active site761For Nsp1-alpha papain-like cysteine proteinase activity By similarity
Active site1461For Nsp1-alpha papain-like cysteine proteinase activity By similarity
Active site2701For Nsp1-beta papain-like cysteine proteinase activity By similarity
Active site3391For Nsp1-beta papain-like cysteine proteinase activity By similarity
Active site4371For Nsp2 cysteine proteinase activity By similarity
Active site5061For Nsp2 cysteine proteinase activity By similarity
Active site18481Charge relay system; for 3C-like serine proteinase activity By similarity
Active site18731Charge relay system; for 3C-like serine proteinase activity By similarity
Active site19271Charge relay system; for 3C-like serine proteinase activity By similarity
Site180 – 1812Cleavage; by autolysis
Site383 – 3842Cleavage; by autolysis
Site1579 – 15802Cleavage; by CP2 Potential
Site1809 – 18102Cleavage; by 3CLSP By similarity
Site2013 – 20142Cleavage; by 3CLSP By similarity
Site2183 – 21842Cleavage; by 3CLSP By similarity
Site2199 – 22002Cleavage; by 3CLSP By similarity
Site2348 – 23492Cleavage; by 3CLSP By similarity
Site2458 – 24592Cleavage; by 3CLSP By similarity
Site3143 – 31442Cleavage; by 3CLSP By similarity
Site31941Involved in mRNA transcription process By similarity
Site3584 – 35852Cleavage; by 3CLSP By similarity
Site3807 – 38082Cleavage; by 3CLSP By similarity

Natural variations

Alternative sequence2504 – 39601457Missing in isoform Replicase polyprotein 1a.
VSP_032893
Natural variant1881H → R in strain: BJ-4.
Natural variant3861K → R in strain: BJ-4.
Natural variant3931S → H in strain: BJ-4.
Natural variant14981S → A in strain: Isolate infectious clone VR-2332.
Natural variant17771T → I in strain: Isolate infectious clone VR-2332.
Natural variant22221D → N in strain: Isolate infectious clone VR-2332.
Natural variant32571G → E in strain: Isolate infectious clone VR-2332.
Natural variant35391C → R in strain: Isolate infectious clone VR-2332.
Natural variant36231S → T in strain: Isolate infectious clone VR-2332.
Natural variant36781G → E in strain: Isolate infectious clone VR-2332.
Natural variant37141G → A in strain: Isolate infectious clone VR-2332.

Experimental info

Mutagenesis761C → S: Complete loss of papain-like cysteine protease activity of nsp1-alpha. Loss of inhibition of IFN-beta production. Loss of inhibition of IRF-3 phosphorylation. Ref.7
Mutagenesis1461H → D: Complete loss of papain-like cysteine protease activity of nsp1-alpha. Ref.7
Mutagenesis2701C → S: No effect on inhibition of IRF-3 phosphorylation. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: F219409DA3F3B73F

FASTA3,960432,887
        10         20         30         40         50         60 
MSGILDRCTC TPNARVFMAE GQVYCTRCLS ARSLLPLNLQ VSELGVLGLF YRPEEPLRWT 

        70         80         90        100        110        120 
LPRAFPTVEC SPAGACWLSA IFPIARMTSG NLNFQQRMVR VAAELYRAGQ LTPAVLKALQ 

       130        140        150        160        170        180 
VYERGCRWYP IVGPVPGVAV FANSLHVSDK PFPGATHVLT NLPLPQRPKP EDFCPFECAM 

       190        200        210        220        230        240 
ATVYDIGHDA VMYVAERKVS WAPRGGDEVK FEAVPGELKL IANRLRTSFP PHHTVDMSKF 

       250        260        270        280        290        300 
AFTAPGCGVS MRVERQHGCL PADTVPEGNC WWSLFDLLPL EVQNKEIRHA NQFGYQTKHG 

       310        320        330        340        350        360 
VSGKYLQRRL QVNGLRAVTD LNGPIVVQYF SVKESWIRHL KLAGEPSYSG FEDLLRIRVE 

       370        380        390        400        410        420 
PNTSPLADKE EKIFRFGSHK WYGAGKRARK ARSCATATVA GRALSVRETR QAKEHEVAGA 

       430        440        450        460        470        480 
NKAEHLKHYS PPAEGNCGWH CISAIANRMV NSKFETTLPE RVRPPDDWAT DEDLVNAIQI 

       490        500        510        520        530        540 
LRLPAALDRN GACTSAKYVL KLEGEHWTVT VTPGMSPSLL PLECVQGCCG HKGGLGSPDA 

       550        560        570        580        590        600 
VEVSGFDPAC LDRLAEVMHL PSSAIPAALA EMSGDSDRSA SPVTTVWTVS QFFARHSGGN 

       610        620        630        640        650        660 
HPDQVRLGKI ISLCQVIEDC CCSQNKTNRV TPEEVAAKID LYLRGATNLE ECLARLEKAR 

       670        680        690        700        710        720 
PPRVIDTSFD WDVVLPGVEA ATQTIKLPQV NQCRALVPVV TQKSLDNNSV PLTAFSLANY 

       730        740        750        760        770        780 
YYRAQGDEVR HRERLTAVLS KLEKVVREEY GLMPTEPGPR PTLPRGLDEL KDQMEEDLLK 

       790        800        810        820        830        840 
LANAQTTSDM MAWAVEQVDL KTWVKNYPRW TPPPPPPKVQ PRKTKPVKSL PERKPVPAPR 

       850        860        870        880        890        900 
RKVGSDCGSP VSLGGDVPNS WEDLAVSSPF DLPTPPEPAT PSSELVIVSS PQCIFRPATP 

       910        920        930        940        950        960 
LSEPAPIPAP RGTVSRPVTP LSEPIPVPAP RRKFQQVKRL SSAAAIPPYQ DEPLDLSASS 

       970        980        990       1000       1010       1020 
QTEYEASPPA PPQSGGVLGV EGHEAEETLS EISDMSGNIK PASVSSSSSL SSVRITRPKY 

      1030       1040       1050       1060       1070       1080 
SAQAIIDSGG PCSGHLQEVK ETCLSVMREA CDATKLDDPA TQEWLSRMWD RVDMLTWRNT 

      1090       1100       1110       1120       1130       1140 
SVYQAICTLD GRLKFLPKMI LETPPPYPCE FVMMPHTPAP SVGAESDLTI GSVATEDVPR 

      1150       1160       1170       1180       1190       1200 
ILEKIENVGE MANQGPLAFS EDKPVDDQLV NDPRISSRRP DESTSAPSAG TGGAGSFTDL 

      1210       1220       1230       1240       1250       1260 
PPSDGADADG GGPFRTVKRK AERLFDQLSR QVFDLVSHLP VFFSRLFYPG GGYSPGDWGF 

      1270       1280       1290       1300       1310       1320 
AAFTLLCLFL CYSYPAFGIA PLLGVFSGSS RRVRMGVFGC WLAFAVGLFK PVSDPVGAAC 

      1330       1340       1350       1360       1370       1380 
EFDSPECRNI LHSFELLKPW DPVRSLVVGP VGLGLAILGR LLGGARCIWH FLLRLGIVAD 

      1390       1400       1410       1420       1430       1440 
CILAGAYVLS QGRCKKCWGS CIRTAPNEVA FNVFPFTRAT RSSLIDLCDR FCAPKGMDPI 

      1450       1460       1470       1480       1490       1500 
FLATGWRGCW AGRSPIEQPS EKPIAFAQLD EKKITARTVV AQPYDPNQAV KCLRVLQSGG 

      1510       1520       1530       1540       1550       1560 
AMVAKAVPKV VKVSAVPFRA PFFPTGVKVD PDCRVVVDPD TFTAALRSGY STTNLVLGVG 

      1570       1580       1590       1600       1610       1620 
DFAQLNGLKI RQISKPSGGG PHLMAALHVA CSMALHMLAG IYVTAVGSCG TGTNDPWCAN 

      1630       1640       1650       1660       1670       1680 
PFAVPGYGPG SLCTSRLCIS QHGLTLPLTA LVAGFGIQEI ALVVLIFVSI GGMAHRLSCK 

      1690       1700       1710       1720       1730       1740 
ADMLCVLLAI ASYVWVPLTW LLCVFPCWLR CFSLHPLTIL WLVFFLISVN MPSGILAMVL 

      1750       1760       1770       1780       1790       1800 
LVSLWLLGRY TNVAGLVTPY DIHHYTSGPR GVAALATAPD GTYLAAVRRA ALTGRTMLFT 

      1810       1820       1830       1840       1850       1860 
PSQLGSLLEG AFRTRKPSLN TVNVIGSSMG SGGVFTIDGK VKCVTAAHVL TGNSARVSGV 

      1870       1880       1890       1900       1910       1920 
GFNQMLDFDV KGDFAIADCP NWQGAAPKTQ FCTDGWTGRA YWLTSSGVEP GVIGKGFAFC 

      1930       1940       1950       1960       1970       1980 
FTACGDSGSP VITEAGELVG VHTGSNKQGG GIVTRPSGQF CNVAPIKLSE LSEFFAGPKV 

      1990       2000       2010       2020       2030       2040 
PLGDVKVGSH IIKDISEVPS DLCALLAAKP ELEGGLSTVQ LLCVFFLLWR MMGHAWTPLV 

      2050       2060       2070       2080       2090       2100 
AVSFFILNEV LPAVLVRSVF SFGMFVLSWL TPWSAQVLMI RLLTAALNRN RWSLAFFSLG 

      2110       2120       2130       2140       2150       2160 
AVTGFVADLA ATQGHPLQAV MNLSTYAFLP RMMVVTSPVP VITCGVVHLL AIILYLFKYR 

      2170       2180       2190       2200       2210       2220 
GPHHILVGDG VFSAAFFLRY FAEGKLREGV SQSCGMNHES LTGALAMRLN DEDLDFLMKW 

      2230       2240       2250       2260       2270       2280 
TDFKCFVSAS NMRNAAGQFI EAAYAKALRV ELAQLVQVDK VRGTLAKLEA FADTVAPQLS 

      2290       2300       2310       2320       2330       2340 
PGDIVVALGH TPVGSIFDLK VGSTKHTLQA IETRVLAGSK MTVARVVDPT PTPPPAPVPI 

      2350       2360       2370       2380       2390       2400 
PLPPKVLENG PNAWGDEDRL NKKKRRRMEA LGIYVMGGKK YQKFWDKNSG DVFYEEVHNN 

      2410       2420       2430       2440       2450       2460 
TDEWECLRVG DPADFDPEKG TLCGHVTIEN KAYHVYTSPS GKKFLVPVNP ENGRVQWEAA 

      2470       2480       2490       2500       2510       2520 
KLSVEQALGM MNVDGELTAK ELEKLKRIID KLQGLTKEQC LNCLAASDLT RCGRGGLVVT 

      2530       2540       2550       2560       2570       2580 
ETAVKIVKFH NRTFTLGPVN LKVASEVELK DAVEHNQHPV ARPIDGGVVL LRSAVPSLID 

      2590       2600       2610       2620       2630       2640 
VLISGADASP KLLAHHGPGN TGIDGTLWDF ESEATKEEVA LSAQIIQACD IRRGDAPEIG 

      2650       2660       2670       2680       2690       2700 
LPYKLYPVRG NPERVKGVLQ NTRFGDIPYK TPSDTGSPVH AAACLTPNAT PVTDGRSVLA 

      2710       2720       2730       2740       2750       2760 
TTMPPGFELY VPTIPASVLD YLDSRPDCPK QLTEHGCEDA ALKDLSKYDL STQGFVLPGV 

      2770       2780       2790       2800       2810       2820 
LRLVRKYLFA HVGKCPPVHR PSTYPAKNSM AGINGNRFPT KDIQSVPEID VLCAQAVREN 

      2830       2840       2850       2860       2870       2880 
WQTVTPCTLK KQYCGKKKTR TILGTNNFIA LAHRAVLSGV TQGFMKKAFN SPIALGKNKF 

      2890       2900       2910       2920       2930       2940 
KELQTPVLGR CLEADLASCD RSTPAIVRWF AANLLYELAC AEEHLPSYVL NCCHDLLVTQ 

      2950       2960       2970       2980       2990       3000 
SGAVTKRGGL SSGDPITSVS NTIYSLVIYA QHMVLSYFKS GHPHGLLFLQ DQLKFEDMLK 

      3010       3020       3030       3040       3050       3060 
VQPLIVYSDD LVLYAESPTM PNYHWWVEHL NLMLGFQTDP KKTAITDSPS FLGCRIINGR 

      3070       3080       3090       3100       3110       3120 
QLVPNRDRIL AALAYHMKAS NVSEYYASAA AILMDSCACL EYDPEWFEEL VVGIAQCARK 

      3130       3140       3150       3160       3170       3180 
DGYSFPGTPF FMSMWEKLRS NYEGKKSRVC GYCGAPAPYA TACGLDVCIY HTHFHQHCPV 

      3190       3200       3210       3220       3230       3240 
TIWCGHPAGS GSCSECKSPV GKGTSPLDEV LEQVPYKPPR TVIMHVEQGL TPLDPGRYQT 

      3250       3260       3270       3280       3290       3300 
RRGLVSVRRG IRGNEVGLPD GDYASTALLP TCKEINMVAV ASNVLRSRFI IGPPGAGKTY 

      3310       3320       3330       3340       3350       3360 
WLLQQVQDGD VIYTPTHQTM LDMIRALGTC RFNVPAGTTL QFPVPSRTGP WVRILAGGWC 

      3370       3380       3390       3400       3410       3420 
PGKNSFLDEA AYCNHLDVLR LLSKTTLTCL GDFKQLHPVG FDSHCYVFDI MPQTQLKTIW 

      3430       3440       3450       3460       3470       3480 
RFGQNICDAI QPDYRDKLMS MVNTTRVTYV EKPVRYGQVL TPYHRDREDD AITIDSSQGA 

      3490       3500       3510       3520       3530       3540 
TFDVVTLHLP TKDSLNRQRA LVAITRARHA IFVYDPHRQL QGLFDLPAKG TPVNLAVHCD 

      3550       3560       3570       3580       3590       3600 
GQLIVLDRNN KECTVAQALG NGDKFRATDK RVVDSLRAIC ADLEGSSSPL PKVAHNLGFY 

      3610       3620       3630       3640       3650       3660 
FSPDLTQFAK LPVELAPHWP VVSTQNNEKW PDRLVASLRP IHKYSRACIG AGYMVGPSVF 

      3670       3680       3690       3700       3710       3720 
LGTPGVVSYY LTKFVKGGAQ VLPETVFSTG RIEVDCREYL DDREREVAAS LPHGFIGDVK 

      3730       3740       3750       3760       3770       3780 
GTTVGGCHHV TSRYLPRVLP KESVAVVGVS SPGKAAKALC TLTDVYLPDL EAYLHPETQS 

      3790       3800       3810       3820       3830       3840 
KCWKMMLDFK EVRLMVWKDK TAYFQLEGRY FTWYQLASYA SYIRVPVNST VYLDPCMGPA 

      3850       3860       3870       3880       3890       3900 
LCNRRVVGST HWGADLAVTP YDYGAKIILS SAYHGEMPPG YKILACAEFS LDDPVKYKHT 

      3910       3920       3930       3940       3950       3960 
WGFESDTAYL YEFTGNGEDW EDYNDAFRAR QEGKIYKATA TSLKFYFPPG PVIEPTLGLN 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

Checksum: D1CCE7E7404CA4E0
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FASTA2,503271,972

References

[1]"Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents."
Nelsen C.J., Murtaugh M.P., Faaberg K.S.
J. Virol. 73:270-280(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]Murtaugh M.P., Faaberg K.S., Nelsen C.J.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Generation of an infectious clone of VR-2332, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus."
Nielsen H.S., Liu G., Nielsen J., Oleksiewicz M.B., Botner A., Storgaard T., Faaberg K.S.
J. Virol. 77:3702-3711(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone VR-2332.
[4]"Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses."
Frias-Staheli N., Giannakopoulos N.V., Kikkert M., Taylor S.L., Bridgen A., Paragas J., Richt J.A., Rowland R.R., Schmaljohn C.S., Lenschow D.J., Snijder E.J., Garcia-Sastre A., Virgin H.W.
Cell Host Microbe 2:404-416(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NSP2.
[5]"Nonstructural protein 1alpha subunit-based inhibition of NF-kappaB activation and suppression of interferon-beta production by porcine reproductive and respiratory syndrome virus."
Song C., Krell P., Yoo D.
Virology 407:268-280(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NSP1-ALPHA, SUBCELLULAR LOCATION.
[6]"Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist."
Chen Z., Lawson S., Sun Z., Zhou X., Guan X., Christopher-Hennings J., Nelson E.A., Fang Y.
Virology 398:87-97(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NSP1-ALPHA, FUNCTION OF NSP1-BETA, PROTEOLYTIC PROCESSING OF POLYPROTEIN, PROTEIN SEQUENCE OF 181-190 AND 384-393.
Strain: Isolate SD23983.
[7]"The nonstructural protein 1 papain-like cysteine protease was necessary for porcine reproductive and respiratory syndrome virus nonstructural protein 1 to inhibit interferon-beta induction."
Shi X., Zhang G., Wang L., Li X., Zhi Y., Wang F., Fan J., Deng R.
DNA Cell Biol. 30:355-362(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NSP1-ALPHA, MUTAGENESIS OF CYS-76; HIS-146 AND CYS-270.
Strain: BJ-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87392 Genomic RNA. Translation: AAD12132.2.
U87392 Genomic RNA. Translation: AAD12125.1. Different initiation.
AY150564 Genomic RNA. Translation: AAO13191.1.
AY150564 Genomic RNA. Translation: AAO13192.1. Different initiation.

3D structure databases

ProteinModelPortalQ9WJB2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR008743. Arterivirus_Nsp2_C33.
IPR023338. Arterivirus_NSP4_peptidase.
IPR027355. AV_MBD_dom.
IPR008741. AV_PCPalpha.
IPR025773. AV_PCPbeta.
IPR008760. EAV_peptidase_S32.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF05410. Peptidase_C31. 1 hit.
PF05411. Peptidase_C32. 1 hit.
PF05412. Peptidase_C33. 1 hit.
PF05579. Peptidase_S32. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS51538. AV_CP. 1 hit.
PS51652. AV_MBD. 1 hit.
PS51493. AV_NSP4_PRO. 1 hit.
PS51539. AV_PCP_ALPHA. 1 hit.
PS51540. AV_PCP_BETA. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOA_PRRSR
AccessionPrimary (citable) accession number: Q9WJB2
Secondary accession number(s): Q80KX0, Q80KX1, Q9WJB3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: May 14, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries