Q9WIK0 (REP7_FBNY1) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 56.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Para-Rep C7 Short name=Rep7 EC=2.7.7.- EC=3.1.21.- EC=3.6.1.3 Alternative name(s): ATP-dependent helicase C7 Replication-associated protein of non-essential DNA C7 | ||
| Gene names |
| ||
| Organism | Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV) [Complete proteome] | ||
| Taxonomic identifier | 291603 [NCBI] | ||
| Taxonomic lineage | Viruses › ssDNA viruses › Nanoviridae › Nanovirus ›  | ||
| Virus host | Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827] Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864] Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885] Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906] |
Protein attributes
| Sequence length | 283 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity. Ref.2 |
| Catalytic activity | ATP + H2O = ADP + phosphate. |
| Cofactor | Divalent metal cations, possibly magnesium or manganese By similarity. |
| Subunit structure | Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity. |
| Subcellular location | Host nucleus Potential. |
| Domain | There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR. |
| Miscellaneous | The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs. |
| Sequence similarities | Belongs to the nanoviridea/circoviridae replication-associated protein family. |
| Caution | This protein is encoded by a subviral DNA that is not present in all isolates of the virus. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 283 | 283 | Para-Rep C7 | PRO_0000222441 | |||||
Regions | |||||||||
| Nucleotide binding | 178 – 180 | 3 | ATP By similarity | ||||||
| Motif | 11 – 14 | 4 | RCR-1 By similarity | ||||||
| Motif | 42 – 47 | 6 | RCR-2 By similarity | ||||||
| Motif | 51 – 71 | 21 | Nuclear localization signal Potential | ||||||
| Motif | 79 – 82 | 4 | RCR-3 By similarity | ||||||
| Motif | 96 – 102 | 7 | Nuclear localization signal Potential | ||||||
Sites | |||||||||
| Active site | 79 | 1 | For DNA cleavage activity By similarity | ||||||
| Metal binding | 36 | 1 | Divalent metal cation Potential | ||||||
| Metal binding | 42 | 1 | Divalent metal cation Potential | ||||||
| Metal binding | 84 | 1 | Divalent metal cation Potential | ||||||
Sequences
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References
| [1] | "Ten distinct circular ssDNA components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome." Katul L., Timchenko T., Gronenborn B., Vetten H.J. J. Gen. Virol. 79:3101-3109(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants." Timchenko T., de Kouchkovsky F., Katul L., David C., Vetten H.J., Gronenborn B. J. Virol. 73:10173-10182(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [3] | "Nanoviruses: genome organisation and protein function." Gronenborn B. Vet. Microbiol. 98:103-109(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ132185 Genomic DNA. Translation: CAB44025.1. |
| RefSeq | NP_619572.1. NC_003565.1. |
3D structure databases | |
| ProteinModelPortal | Q9WIK0. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 993378. |
Family and domain databases | |
| InterPro | IPR000605. Helicase_SF3_ssDNA/RNA_vir. IPR003365. Viral_rep_N. [Graphical view] |
| Pfam | PF00910. RNA_helicase. 1 hit. PF02407. Viral_Rep. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | REP7_FBNY1 | ||||||||
| Accession | Primary (citable) accession number: Q9WIK0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |