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Q9WIK0 (REP7_FBNY1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Para-Rep C7

Short name=Rep7
EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
ATP-dependent helicase C7
Replication-associated protein of non-essential DNA C7
Gene names
Name:C7
OrganismFaba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV) [Complete proteome]
Taxonomic identifier291603 [NCBI]
Taxonomic lineageVirusesssDNA virusesNanoviridaeNanovirus
Virus hostCicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity. Ref.2

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Caution

This protein is encoded by a subviral DNA that is not present in all isolates of the virus.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Para-Rep C7
PRO_0000222441

Regions

Nucleotide binding178 – 1803ATP By similarity
Motif11 – 144RCR-1 By similarity
Motif42 – 476RCR-2 By similarity
Motif51 – 7121Nuclear localization signal Potential
Motif79 – 824RCR-3 By similarity
Motif96 – 1027Nuclear localization signal Potential

Sites

Active site791For DNA cleavage activity By similarity
Metal binding361Divalent metal cation Potential
Metal binding421Divalent metal cation Potential
Metal binding841Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q9WIK0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: EE49FFB3D450C060

FASTA28333,092
        10         20         30         40         50         60 
MPSIRATHWC FTLNFSGSIP EINWTADVQY SIWQHERVGH DHLQGYIQMN KHVTLKKMKE 

        70         80         90        100        110        120 
LLPGAHLEMA KAPKKAIEYC QKKESAIAGP WEYGTWISSG SHKRKLMERF EDGPEEMKLE 

       130        140        150        160        170        180 
DPGLYRRCLS RVQMKKIRES CTWNFDLRPW QDELLKTIEQ EPDDRTIIWV YGPHGGEGKS 

       190        200        210        220        230        240 
AFAKYLTLKE GWWYTAGGKA TDMLYSYSLD PTCHVCIDIP RCTREEYINY SVIEQIKNRV 

       250        260        270        280 
IINTKYEPCT IRDDGHNVHV IVFCNFLPDV TRISEDRIKI INC 

« Hide

References

[1]"Ten distinct circular ssDNA components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome."
Katul L., Timchenko T., Gronenborn B., Vetten H.J.
J. Gen. Virol. 79:3101-3109(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants."
Timchenko T., de Kouchkovsky F., Katul L., David C., Vetten H.J., Gronenborn B.
J. Virol. 73:10173-10182(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Nanoviruses: genome organisation and protein function."
Gronenborn B.
Vet. Microbiol. 98:103-109(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ132185 Genomic DNA. Translation: CAB44025.1.

3D structure databases

ProteinModelPortalQ9WIK0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameREP7_FBNY1
AccessionPrimary (citable) accession number: Q9WIK0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 1, 1999
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families