Reviewed,
UniProtKB/Swiss-Prot Q9WIJ5 (REP2_FBNY1)
Last modified
June 16, 2009.
Version 41.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Master replication protein Short name=M-Rep EC=2.7.7.- EC=3.1.21.- EC=3.6.1.3 Alternative name(s): ATP-dependent helicase C2 Replication-associated protein 2 Short name=Rep2 | ||
| Gene names |
| ||
| Organism | Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV) | ||
| Taxonomic identifier | 291603 [NCBI] | ||
| Taxonomic lineage | Viruses › ssDNA viruses › Nanoviridae › Nanovirus | ||
| Virus host | Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827] Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864] Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885] Vicia faba (Broad bean) [TaxID: 3906] |
Protein attributes
| Sequence length | 286 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. |
| Catalytic activity | ATP + H2O = ADP + phosphate. |
| Cofactor | Divalent metal cations, possibly magnesium or manganese Probable. |
| Subcellular location | Host nucleus Potential. |
| Domain | There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR. |
| Miscellaneous | The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs. |
| Sequence similarities | Belongs to the nanovirus/circovirus replication-associated protein family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 286 | 286 | Master replication protein | PRO_0000222440 | |||||
Regions | |||||||||
| Nucleotide binding | 186 – 188 | 3 | ATP Probable | ||||||
| Motif | 10 – 13 | 4 | RCR-1 By similarity | ||||||
| Motif | 41 – 46 | 6 | RCR-2 By similarity | ||||||
| Motif | 50 – 70 | 21 | Nuclear localization signal Potential | ||||||
| Motif | 79 – 82 | 4 | RCR-3 By similarity | ||||||
| Motif | 96 – 102 | 7 | Nuclear localization signal Potential | ||||||
Sites | |||||||||
| Active site | 79 | 1 | For DNA cleavage activity | ||||||
| Metal binding | 33 | 1 | Divalent metal cation Potential | ||||||
| Metal binding | 41 | 1 | Divalent metal cation Potential | ||||||
| Metal binding | 84 | 1 | Divalent metal cation Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 79 | 1 | Y → F: Complete loss of DNA cleavage and nucleotidyl transfer activity. Ref.2 | ||||||
| Mutagenesis | 187 | 1 | K → A: Complete loss of ATPase activity. Ref.2 | ||||||
Sequences
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References
| [1] | "Analysis of six DNA components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes." Katul L., Maiss E., Morozov S.Y., Vetten H.J. Virology 233:247-259(1997) [PubMed: 9217049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants." Timchenko T., de Kouchkovsky F., Katul L., David C., Vetten H.J., Gronenborn B. J. Virol. 73:10173-10182(1999) [PubMed: 10559333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], COFACTOR, MUTAGENESIS OF TYR-79 AND LYS-187. |
| [3] | "Nanoviruses: genome organisation and protein function." Gronenborn B. Vet. Microbiol. 98:103-109(2004) [PubMed: 14741122] [Abstract] Cited for: REVIEW. |
Cross-references
Sequence databases | |
|---|---|
| AJ132180 Genomic DNA. Translation: CAB44020.1. | |
| RefSeq | NP_619567.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 993373. |
Family and domain databases | |
| InterPro | IPR000605. Helicase_SF3_ssDNA/RNA_vir. IPR013628. Viral_Rep_C. IPR003365. Viral_rep_N. [Graphical view] |
| Pfam | PF00910. RNA_helicase. 1 hit. PF02407. Viral_Rep. 1 hit. PF08419. Viral_Rep_C. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | REP2_FBNY1 | ||||||||
| Accession | Primary (citable) accession number: Q9WIJ5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Virus (Virus annotation project) | ||||||||
