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Q9WIJ5

- MREP_FBNY1

UniProt

Q9WIJ5 - MREP_FBNY1

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Protein
Master replication protein
Gene
DNA-R, C2
Organism
Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Divalent metal cations, possibly magnesium or manganese Inferred.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi33 – 331Divalent metal cation Reviewed prediction
Metal bindingi41 – 411Divalent metal cation Reviewed prediction
Active sitei79 – 791For DNA cleavage activity
Metal bindingi84 – 841Divalent metal cation Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi186 – 1883ATP Inferred

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity, uncoupled Source: InterPro
  3. DNA binding Source: UniProtKB-KW
  4. RNA binding Source: InterPro
  5. RNA helicase activity Source: InterPro
  6. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. metal ion binding Source: UniProtKB-KW
  8. nucleotidyltransferase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein-DNA covalent cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Master replication protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Short name:
M-Rep
Alternative name(s):
ATP-dependent helicase C2
Replication-associated protein 2
Short name:
Rep2
Gene namesi
Name:DNA-R
Synonyms:C2
OrganismiFaba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV)
Taxonomic identifieri291603 [NCBI]
Taxonomic lineageiVirusesssDNA virusesNanoviridaeNanovirus
Virus hostiCicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]
ProteomesiUP000008665: Genome

Subcellular locationi

Host nucleus Reviewed prediction

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi79 – 791Y → F: Complete loss of DNA cleavage and nucleotidyl transfer activity. 1 Publication
Mutagenesisi187 – 1871K → A: Complete loss of ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Master replication protein
PRO_0000222440Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Homooligomer Reviewed prediction. Rep binds to repeated DNA motifs (iterons) By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9WIJ5.
SMRiQ9WIJ5. Positions 2-95.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 134RCR-1 By similarity
Motifi41 – 466RCR-2 By similarity
Motifi50 – 7021Nuclear localization signal Reviewed prediction
Add
BLAST
Motifi79 – 824RCR-3 By similarity
Motifi96 – 1027Nuclear localization signal Reviewed prediction

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WIJ5-1 [UniParc]FASTAAdd to Basket

« Hide

MARQVICWCF TLNNPLSPLS LHDSMKYLVY QTEQGEAGNI HFQGYIEMKK    50
RTSLAGMKKL IPGAHFEKRR GTQGEARAYS MKEDPRLEGP WEYEEFVPTI 100
EDKLREVMND MKITGKRPIE YIEECCNTYD KSASTLREFR GELKKKKAIS 150
SWELQRKPWM DEVDALLQER DGRRIIWVYG PQGGEGKTSY AKHLVKTRDA 200
FYSTGGKTAD IAFAWDHQEL VLFDFPRSFE EYVNYGVIEQ LKNGIIQSGK 250
YQSVIKYSDY VEVIVFANFT PRSGMFSEDR IVYVYA 286
Length:286
Mass (Da):33,267
Last modified:November 1, 1999 - v1
Checksum:iA20156C5162A2F19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ132180 Genomic DNA. Translation: CAB44020.1.
RefSeqiNP_619567.1. NC_003560.1.

Genome annotation databases

GeneIDi993373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ132180 Genomic DNA. Translation: CAB44020.1 .
RefSeqi NP_619567.1. NC_003560.1.

3D structure databases

ProteinModelPortali Q9WIJ5.
SMRi Q9WIJ5. Positions 2-95.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 993373.

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view ]
Pfami PF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of six DNA components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes."
    Katul L., Maiss E., Morozov S.Y., Vetten H.J.
    Virology 233:247-259(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants."
    Timchenko T., de Kouchkovsky F., Katul L., David C., Vetten H.J., Gronenborn B.
    J. Virol. 73:10173-10182(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], COFACTOR, MUTAGENESIS OF TYR-79 AND LYS-187.
  3. "Nanoviruses: genome organisation and protein function."
    Gronenborn B.
    Vet. Microbiol. 98:103-109(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiMREP_FBNY1
AccessioniPrimary (citable) accession number: Q9WIJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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