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Q9WAB2 (LMBD1_REOVL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein lambda-1

Short name=Lambda1
EC=3.6.4.13
Alternative name(s):
ATP-dependent DNA helicase lambda-1
Lambda1(Hel)
Gene names
Name:L3
OrganismReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]
Taxonomic identifier10884 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length1275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inner capsid (core) component. Displays NTPase, RNA 5'-triphosphatase (RTPase) and RNA helicase activities and probably participates in transcription of the viral genome. Helicase activity might be involved in unwinding or reannealing dsRNA during RNA synthesis. RTPase enzymatic activity represents the first step in RNA capping, which yields a 5'-diphosphorylated plus-strand RNA By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Interacts with protein mu-NS; in viral inclusions. Ref.2

Subcellular location

Virion Potential. Note: Found in the inner capsid (120 copies) By similarity.

Sequence similarities

Belongs to the orthoreovirus lambda-1 protein family.

Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
   Biological processmRNA capping
mRNA processing
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainZinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionHelicase
Hydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process7-methylguanosine mRNA capping

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12751275Inner capsid protein lambda-1
PRO_0000344998

Regions

Zinc finger181 – 20323C2H2-type

Sequences

Sequence LengthMass (Da)Tools
Q9WAB2 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D53864D04E374BF1

FASTA1,275141,880
        10         20         30         40         50         60 
MKRIPRKTKG KSSGKGNDST ERSDDGSSQL RDKQNNKAGP ATTEPGTSNR EQYRARPGIA 

        70         80         90        100        110        120 
SVQRATESAE LPMKNNDEGT PDKKGNTRGD LVNEHSEAKD EADEATQKQA KDTDKSKAQV 

       130        140        150        160        170        180 
TYSDTGINNA NELSRSGNVD NEGGSNQKPM STRIAEATSA IVSKHPARVG LPPTASSGHG 

       190        200        210        220        230        240 
YQCHVCSAVL FSPLDLDAHV ASHGLHGNMT LTSSEIQRHI TEFISSWQNH PIVQVSADVE 

       250        260        270        280        290        300 
NKKTAQLLHA DTPRLVTWDA GLCTSFKIVP IVPAQVPQDV LAYTFFTSSY AIQSPFPEAA 

       310        320        330        340        350        360 
VSRIVVHTRW ASNVDFDRDS SVIMAPPTEN NIHLFKQLLN TETLSVRGAN PLMFRANVLH 

       370        380        390        400        410        420 
MLLEFVLDNL YLNRHTGFSQ DHTPFTEGAN LRSLPGPDAE KWYSIMYPTR MGTPNVSKIC 

       430        440        450        460        470        480 
NFVASCVRNR VGRFDRAQMM NGAMSEWVDV FETSDALTVS IRGRWMARLA RMNINPTEIE 

       490        500        510        520        530        540 
WALTECAQGY VTVTSPYAPS VNRLMPYRIS NAERQISQII RIMNIGNNAT VIQPVLQDIS 

       550        560        570        580        590        600 
VLLQRISPLQ IDPTIISNTM STVSESTTQT LSPASSILGK LRPSNSDFSS FRVALAGWLY 

       610        620        630        640        650        660 
NGVVTTVIDD SSYPKDGGSV TSLENLWDFF ILALALPLTT DPCAPVKAFM TLANMMVGFE 

       670        680        690        700        710        720 
TIPMDNQIYT QSRRASAFST PHTWPRCFMN IQLISPIDAP ILRQWAEIIH RYWPNPSQIR 

       730        740        750        760        770        780 
YGAPNVFGSA NLFTPPEVLL LPIDHQPANV TTPTLDFTNE LTNWRARVCE LMKNLVDNQR 

       790        800        810        820        830        840 
YQPGWTQSLV SSMRGTLDKL KLIKSMTPMY LQQLAPVELA VIAPMLPFPP FQVPYVRLDR 

       850        860        870        880        890        900 
DRVPTMVGVT RQSRDTITQP ALSLSTTNTT VGVPLALDAR AITVALLSGK YPPDLVTNVW 

       910        920        930        940        950        960 
YADAIYPMYA DTEVFSNLQR DMITCEAVQT LVTLVAQISE TQYPVDRYLD WIPSLRASAA 

       970        980        990       1000       1010       1020 
TAATFAEWVN TSMKTAFDLS DMLLEPLLSG DPRMTQLAIQ YQQYNGRTFN VIPEMPGSVI 

      1030       1040       1050       1060       1070       1080 
ADCVQLTAEV FNHEYNLFGI ARGDIIIGRV QSTHLWSPLA PPPDLVFDRD TPGVHIFGRD 

      1090       1100       1110       1120       1130       1140 
CRISFGMNGA APMIRDETGM MVPFEGNWIF PLALWQMNTR YFNQQFDAWI KTGELRIRIE 

      1150       1160       1170       1180       1190       1200 
MGAYPYMLHY YDPRQYANAW NLTSAWLEEI TPTSIPSVPF MVPISSDHDI SSAPAVQYII 

      1210       1220       1230       1240       1250       1260 
STEYNDRSLF CTNSSSPQTI AGPDKHIPVE RYNILTNPDA PPTQIQLPEV VDLYNVVTRY 

      1270 
AYETPPITAV VMGVP 

« Hide

References

[1]"Mammalian reovirus L3 gene sequences and evidence for a distinct amino-terminal region of the lambda1 protein."
Harrison S.J., Farsetta D.L., Kim J., Noble S., Broering T.J., Nibert M.L.
Virology 258:54-64(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PROTEIN MU-NS.
[3]"Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution."
Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S.
Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF129820 mRNA. Translation: AAD42304.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CSEelectron microscopy7.00V/W1-1275[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9WAB2.

Entry information

Entry nameLMBD1_REOVL
AccessionPrimary (citable) accession number: Q9WAB2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references