Q9WAB2 (LMBD1_REOVL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner capsid protein lambda-1
Short name=Lambda1
EC=3.6.4.13

Alternative name(s):
ATP-dependent DNA helicase lambda-1
Lambda1(Hel)

Gene names

Name:

L3

Organism

Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]

Taxonomic identifier

10884 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 1

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	1275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inner capsid (core) component. Displays NTPase, RNA 5'-triphosphatase (RTPase) and RNA helicase activities and probably participates in transcription of the viral genome. Helicase activity might be involved in unwinding or reannealing dsRNA during RNA synthesis. RTPase enzymatic activity represents the first step in RNA capping, which yields a 5'-diphosphorylated plus-strand RNA By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Interacts with protein mu-NS; in viral inclusions. Ref.2
Subcellular location	Virion Potential. Note: Found in the inner capsid (120 copies) By similarity.
Sequence similarities	Belongs to the orthoreovirus lambda-1 protein family. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Virion
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Capsid protein Helicase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1275	1275	Inner capsid protein lambda-1		PRO_0000344998
Regions
	Zinc finger	181 – 203	23	C2H2-type

Sequences

	Sequence		Length	Mass (Da)
	Q9WAB2 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D53864D04E374BF1	FASTA	1,275	141,880
	10 20 30 40 50 60 MKRIPRKTKG KSSGKGNDST ERSDDGSSQL RDKQNNKAGP ATTEPGTSNR EQYRARPGIA 70 80 90 100 110 120 SVQRATESAE LPMKNNDEGT PDKKGNTRGD LVNEHSEAKD EADEATQKQA KDTDKSKAQV 130 140 150 160 170 180 TYSDTGINNA NELSRSGNVD NEGGSNQKPM STRIAEATSA IVSKHPARVG LPPTASSGHG 190 200 210 220 230 240 YQCHVCSAVL FSPLDLDAHV ASHGLHGNMT LTSSEIQRHI TEFISSWQNH PIVQVSADVE 250 260 270 280 290 300 NKKTAQLLHA DTPRLVTWDA GLCTSFKIVP IVPAQVPQDV LAYTFFTSSY AIQSPFPEAA 310 320 330 340 350 360 VSRIVVHTRW ASNVDFDRDS SVIMAPPTEN NIHLFKQLLN TETLSVRGAN PLMFRANVLH 370 380 390 400 410 420 MLLEFVLDNL YLNRHTGFSQ DHTPFTEGAN LRSLPGPDAE KWYSIMYPTR MGTPNVSKIC 430 440 450 460 470 480 NFVASCVRNR VGRFDRAQMM NGAMSEWVDV FETSDALTVS IRGRWMARLA RMNINPTEIE 490 500 510 520 530 540 WALTECAQGY VTVTSPYAPS VNRLMPYRIS NAERQISQII RIMNIGNNAT VIQPVLQDIS 550 560 570 580 590 600 VLLQRISPLQ IDPTIISNTM STVSESTTQT LSPASSILGK LRPSNSDFSS FRVALAGWLY 610 620 630 640 650 660 NGVVTTVIDD SSYPKDGGSV TSLENLWDFF ILALALPLTT DPCAPVKAFM TLANMMVGFE 670 680 690 700 710 720 TIPMDNQIYT QSRRASAFST PHTWPRCFMN IQLISPIDAP ILRQWAEIIH RYWPNPSQIR 730 740 750 760 770 780 YGAPNVFGSA NLFTPPEVLL LPIDHQPANV TTPTLDFTNE LTNWRARVCE LMKNLVDNQR 790 800 810 820 830 840 YQPGWTQSLV SSMRGTLDKL KLIKSMTPMY LQQLAPVELA VIAPMLPFPP FQVPYVRLDR 850 860 870 880 890 900 DRVPTMVGVT RQSRDTITQP ALSLSTTNTT VGVPLALDAR AITVALLSGK YPPDLVTNVW 910 920 930 940 950 960 YADAIYPMYA DTEVFSNLQR DMITCEAVQT LVTLVAQISE TQYPVDRYLD WIPSLRASAA 970 980 990 1000 1010 1020 TAATFAEWVN TSMKTAFDLS DMLLEPLLSG DPRMTQLAIQ YQQYNGRTFN VIPEMPGSVI 1030 1040 1050 1060 1070 1080 ADCVQLTAEV FNHEYNLFGI ARGDIIIGRV QSTHLWSPLA PPPDLVFDRD TPGVHIFGRD 1090 1100 1110 1120 1130 1140 CRISFGMNGA APMIRDETGM MVPFEGNWIF PLALWQMNTR YFNQQFDAWI KTGELRIRIE 1150 1160 1170 1180 1190 1200 MGAYPYMLHY YDPRQYANAW NLTSAWLEEI TPTSIPSVPF MVPISSDHDI SSAPAVQYII 1210 1220 1230 1240 1250 1260 STEYNDRSLF CTNSSSPQTI AGPDKHIPVE RYNILTNPDA PPTQIQLPEV VDLYNVVTRY 1270 AYETPPITAV VMGVP

References

[1]	"Mammalian reovirus L3 gene sequences and evidence for a distinct amino-terminal region of the lambda1 protein." Harrison S.J., Farsetta D.L., Kim J., Noble S., Broering T.J., Nibert M.L. Virology 258:54-64(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions." Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L. J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PROTEIN MU-NS.
[3]	"Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution." Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S. Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF129820 mRNA. Translation: AAD42304.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CSE	electron microscopy	7.00	V/W	1-1275	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9WAB2.

Entry information

Entry name

LMBD1_REOVL

Accession

Primary (citable) accession number: Q9WAB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program