ID   LDHA_AMBME              Reviewed;         333 AA.
AC   Q9W7M6;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   16-JUN-2009, entry version 50.
DE   RecName: Full=L-lactate dehydrogenase A chain;
DE            Short=LDH-A;
DE            EC=1.1.1.27;
GN   Name=LDHA;
OS   Ambystoma mexicanum (Axolotl).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Caudata; Salamandroidea; Ambystomatidae;
OC   Ambystoma.
OX   NCBI_TaxID=8296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L.;
RT   "Molecular evolution of vertebrate lactate dehydrogenase isozymes by
RT   gene duplication.";
RL   Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AF070998; AAD40735.1; -; mRNA.
DR   HSSP; P00338; 1I10.
DR   SMR; Q9W7M6; 2-333.
DR   HOVERGEN; Q9W7M6; -.
DR   BRENDA; 1.1.1.27; 1113.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:EC.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR011304; L-lactate_DH.
DR   InterPro; IPR018177; L-lactate_DH_AS.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    333       L-lactate dehydrogenase A chain.
FT                                /FTId=PRO_0000168456.
FT   NP_BIND      30     58       NAD (By similarity).
FT   ACT_SITE    194    194       Proton acceptor (By similarity).
FT   BINDING     100    100       NAD (By similarity).
FT   BINDING     107    107       Substrate (By similarity).
FT   BINDING     139    139       NAD or substrate (By similarity).
FT   BINDING     170    170       Substrate (By similarity).
FT   BINDING     249    249       Substrate (By similarity).
SQ   SEQUENCE   333 AA;  36631 MW;  EF97E41E545492C7 CRC64;
     MSCMKEQLII NILKDEHAPA QNKITVVGVG AVGMACAMSI LMKDLADELA LVDVIEDKLK
     GEMMDLQHGS LFLRTPKIVS GKDYSVTANS KLVIVTAGAR QQEGESRLNL VQRNVNIFKF
     IIPNVVKYSP DATLLVVSNP VDVLTYVAWK ISGFPKHRVI GSGCNLDSAR FRYLMGEKLG
     VHAQSCHGWV VGEHGDSSVP VWSGVNVAGV SLQTLNPELG TDADKENWKE VHKQVVESAY
     EVIKLKGYTS WAIGLSVADL AETIMKNLRR VHPVSTKVKG LYGVHEDVFL SVPCVLGNQG
     ITDVVKMTLK PEEEDRLRKS SDTLWGIQKE LHF
//