Reviewed, UniProtKB/Swiss-Prot Q9W7M6 (LDHA_AMBME)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27

Gene names

Name:

LDHA

Organism

Ambystoma mexicanum (Axolotl)

Taxonomic identifier

8296 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Caudata › Salamandroidea › Ambystomatidae › Ambystoma

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	L-lactate dehydrogenase A chain		PRO_0000168456
Regions
	Nucleotide binding	30 – 58	29	NAD By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	100	1	NAD By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	139	1	NAD or substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9W7M6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: EF97E41E545492C7	FASTA	333	36,631
	10 20 30 40 50 60 MSCMKEQLII NILKDEHAPA QNKITVVGVG AVGMACAMSI LMKDLADELA LVDVIEDKLK 70 80 90 100 110 120 GEMMDLQHGS LFLRTPKIVS GKDYSVTANS KLVIVTAGAR QQEGESRLNL VQRNVNIFKF 130 140 150 160 170 180 IIPNVVKYSP DATLLVVSNP VDVLTYVAWK ISGFPKHRVI GSGCNLDSAR FRYLMGEKLG 190 200 210 220 230 240 VHAQSCHGWV VGEHGDSSVP VWSGVNVAGV SLQTLNPELG TDADKENWKE VHKQVVESAY 250 260 270 280 290 300 EVIKLKGYTS WAIGLSVADL AETIMKNLRR VHPVSTKVKG LYGVHEDVFL SVPCVLGNQG 310 320 330 ITDVVKMTLK PEEEDRLRKS SDTLWGIQKE LHF

References

[1]	"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication." Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF070998 mRNA. Translation: AAD40735.1.
3D structure databases
HSSP	HSSP built from PDB template 1I10 based on UniProtKB P00338.
SMR	Q9W7M6. Positions 2-333.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9W7M6.
Enzyme and pathway databases
BRENDA	1.1.1.27. 1113.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_AMBME

Accession

Primary (citable) accession number: Q9W7M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)