Alpha-enolase

Functionⁱ

Catalytic activityⁱ

2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.

Cofactorⁱ

Mg²⁺By similarityNote: Binds two Mg²⁺ per subunit. Required for catalysis and for stabilizing the dimer.By similarity

Pathwayⁱ: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

no protein annotated in this organism
no protein annotated in this organism
no protein annotated in this organism
Alpha-enolase
no protein annotated in this organism

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	40	Magnesium 1By similarity	1
Binding siteⁱ	158	SubstrateBy similarity	1
Binding siteⁱ	167	SubstrateBy similarity	1
Active siteⁱ	210	Proton donorBy similarity	1
Metal bindingⁱ	245	Magnesium 2By similarity	1
Metal bindingⁱ	293	Magnesium 2By similarity	1
Binding siteⁱ	293	SubstrateBy similarity	1
Metal bindingⁱ	318	Magnesium 2By similarity	1
Binding siteⁱ	318	SubstrateBy similarity	1
Active siteⁱ	343	Proton acceptorBy similarity	1
Binding siteⁱ	394	SubstrateBy similarity	1

GO - Molecular functionⁱ

magnesium ion binding Source: InterPro
phosphopyruvate hydratase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

glycolytic process Source: UniProtKB-UniPathway

Complete GO annotation...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glycolysis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187.

UniPathwayⁱ

UPA00109; UER00187.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha-enolase (EC:4.2.1.11) Alternative name(s): 2-phospho-D-glycerate hydro-lyase Phosphopyruvate hydratase
Organismⁱ	Sceloporus undulatus (Eastern fence lizard) (Stellio undulatus)
Taxonomic identifierⁱ	8520 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Iguania › Iguanidae › Phrynosomatinae › Sceloporus

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

phosphopyruvate hydratase complex Source: InterPro

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000134104	2 – 434	Alpha-enolaseAdd BLAST		433

Proteomic databases

PRIDEⁱ	Q9W7L2.

PRIDEⁱ

Q9W7L2.

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9W7L2.
SMRⁱ	Q9W7L2.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	370 – 373	Substrate bindingBy similarity		4

Sequence similaritiesⁱ

Belongs to the enolase family.Curated

Phylogenomic databases

HOVERGENⁱ	HBG000067.

HOVERGENⁱ

HBG000067.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313. enolase. 1 hit.
Gene3Dⁱ	3.20.20.120. 1 hit. 3.30.390.10. 1 hit.
HAMAPⁱ	MF_00318. Enolase. 1 hit.
InterProⁱ	View protein in InterPro IPR000941. Enolase. IPR034390. Enolase-like_superfamily. IPR020810. Enolase_C. IPR029065. Enolase_C-like. IPR020809. Enolase_CS. IPR020811. Enolase_N. IPR029017. Enolase_N-like.
PANTHERⁱ	PTHR11902. PTHR11902. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit.
PIRSFⁱ	PIRSF001400. Enolase. 1 hit.
PRINTSⁱ	PR00148. ENOLASE.
SFLDⁱ	SFLDG00178. enolase. 1 hit. SFLDS00001. Enolase. 1 hit.
SMARTⁱ	View protein in SMART SM01192. Enolase_C. 1 hit. SM01193. Enolase_N. 1 hit.
SUPFAMⁱ	SSF51604. SSF51604. 1 hit. SSF54826. SSF54826. 1 hit.
TIGRFAMsⁱ	TIGR01060. eno. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00164. ENOLASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9W7L2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSILKLHARE IFDSRGNPTV EVDLYTNKGL FRAAVPSGAS TGIYEALELR 
        60         70         80         90        100
DNDKTRFLGK GVSKAVEHVN KTIAPALVNK KVNVVEQEKI DKLMLEMDGT 
       110        120        130        140        150
ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN EDVILPVPAF 
       160        170        180        190        200
NVINGGSHAG NKLAMQEFMI LPVGAENFKE AMRIGAEVYQ NLKNVIKEKY 
       210        220        230        240        250
GKDATNVGDE GGFAPNILEN KEALELLKSA ISKAGYTDKI VIGMDVAASE 
       260        270        280        290        300
FYRDGKYDLD FKSPDDPSRY ITPDQLSDLY KGFIKNYPVV SIEDPFDQHD 
       310        320        330        340        350
WAAWKKFTAS AGIQVVGDDL TVTNPRRITK AVEEKSCNCL LLKVNQIGSV 
       360        370        380        390        400
TESLQACKLA QTNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR 
       410        420        430 
SERLAKYNQI LRIEEELGSK GRFAGRNFRN PRVN

Length:434

Mass (Da):47,493

Last modified:January 23, 2007 - v3

Checksum:ⁱ6E377E0F0A767E11

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF072587 mRNA. Translation: AAD41644.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF072587 mRNA. Translation: AAD41644.1.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF072587 mRNA. Translation: AAD41644.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF072587 mRNA. Translation: AAD41644.1.

3D structure databases

ProteinModelPortalⁱ	Q9W7L2.
SMRⁱ	Q9W7L2.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	Q9W7L2.

PRIDEⁱ

Q9W7L2.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

HOVERGENⁱ	HBG000067.

HOVERGENⁱ

HBG000067.

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187.

UniPathwayⁱ

UPA00109; UER00187.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313. enolase. 1 hit.
Gene3Dⁱ	3.20.20.120. 1 hit. 3.30.390.10. 1 hit.
HAMAPⁱ	MF_00318. Enolase. 1 hit.
InterProⁱ	View protein in InterPro IPR000941. Enolase. IPR034390. Enolase-like_superfamily. IPR020810. Enolase_C. IPR029065. Enolase_C-like. IPR020809. Enolase_CS. IPR020811. Enolase_N. IPR029017. Enolase_N-like.
PANTHERⁱ	PTHR11902. PTHR11902. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit.
PIRSFⁱ	PIRSF001400. Enolase. 1 hit.
PRINTSⁱ	PR00148. ENOLASE.
SFLDⁱ	SFLDG00178. enolase. 1 hit. SFLDS00001. Enolase. 1 hit.
SMARTⁱ	View protein in SMART SM01192. Enolase_C. 1 hit. SM01193. Enolase_N. 1 hit.
SUPFAMⁱ	SSF51604. SSF51604. 1 hit. SSF54826. SSF54826. 1 hit.
TIGRFAMsⁱ	TIGR01060. eno. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00164. ENOLASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENOA_SCEUN
Accessionⁱ	Q9W7L2Primary (citable) accession number: Q9W7L2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
	Last sequence update:	January 23, 2007
	Last modified:	April 12, 2017
	This is version 79 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9W7L2 (ENOA_SCEUN)

Your basket is currently empty.

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ