Alpha-enolase

Functionⁱ

Catalytic activityⁱ

2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.

Cofactorⁱ

Mg²⁺By similarityNote: Binds two Mg²⁺ per subunit. Required for catalysis and for stabilizing the dimer.By similarity

Pathwayⁱ: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

no protein annotated in this organism
no protein annotated in this organism
no protein annotated in this organism
Alpha-enolase
no protein annotated in this organism

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	40	Magnesium 1By similarity	1
Binding siteⁱ	158	SubstrateBy similarity	1
Binding siteⁱ	167	SubstrateBy similarity	1
Active siteⁱ	210	Proton donorBy similarity	1
Metal bindingⁱ	245	Magnesium 2By similarity	1
Metal bindingⁱ	293	Magnesium 2By similarity	1
Binding siteⁱ	293	SubstrateBy similarity	1
Metal bindingⁱ	318	Magnesium 2By similarity	1
Binding siteⁱ	318	SubstrateBy similarity	1
Active siteⁱ	343	Proton acceptorBy similarity	1
Binding siteⁱ	394	SubstrateBy similarity	1

GO - Molecular functionⁱ

magnesium ion binding Source: InterPro
phosphopyruvate hydratase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glycolytic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glycolysis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187

UniPathwayⁱ

UPA00109; UER00187

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha-enolase (EC:4.2.1.11) Alternative name(s): 2-phospho-D-glycerate hydro-lyase Phosphopyruvate hydratase
Organismⁱ	Sceloporus undulatus (Eastern fence lizard) (Stellio undulatus)
Taxonomic identifierⁱ	8520 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Iguania › Phrynosomatidae › Phrynosomatinae › Sceloporus

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000134104	2 – 434	Alpha-enolaseAdd BLAST		433

Proteomic databases

PRIDEⁱ	Q9W7L2

PRIDEⁱ

Q9W7L2

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9W7L2
SMRⁱ	Q9W7L2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	370 – 373	Substrate bindingBy similarity		4

Sequence similaritiesⁱ

Belongs to the enolase family.Curated

Phylogenomic databases

HOVERGENⁱ	HBG000067

HOVERGENⁱ

HBG000067

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.1201 hit 3.30.390.101 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9W7L2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSILKLHARE IFDSRGNPTV EVDLYTNKGL FRAAVPSGAS TGIYEALELR 
        60         70         80         90        100
DNDKTRFLGK GVSKAVEHVN KTIAPALVNK KVNVVEQEKI DKLMLEMDGT 
       110        120        130        140        150
ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN EDVILPVPAF 
       160        170        180        190        200
NVINGGSHAG NKLAMQEFMI LPVGAENFKE AMRIGAEVYQ NLKNVIKEKY 
       210        220        230        240        250
GKDATNVGDE GGFAPNILEN KEALELLKSA ISKAGYTDKI VIGMDVAASE 
       260        270        280        290        300
FYRDGKYDLD FKSPDDPSRY ITPDQLSDLY KGFIKNYPVV SIEDPFDQHD 
       310        320        330        340        350
WAAWKKFTAS AGIQVVGDDL TVTNPRRITK AVEEKSCNCL LLKVNQIGSV 
       360        370        380        390        400
TESLQACKLA QTNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR 
       410        420        430 
SERLAKYNQI LRIEEELGSK GRFAGRNFRN PRVN

Length:434

Mass (Da):47,493

Last modified:January 23, 2007 - v3

Checksum:ⁱ6E377E0F0A767E11

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF072587 mRNA Translation: AAD41644.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF072587 mRNA Translation: AAD41644.1

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9W7L2	P17182 Q5FW97 A1L3K7 B1ARR7 B1ARR6 A0A0P6CCU8 A0A212CQF6 A6YLM5 B6E3I8 G5D915 +381	Mus musculus (Mouse) Xenopus laevis (African clawed frog) Daphnia magna Cervus elaphus hippelaphus (European red deer) Cervus elaphus (Red deer) Ovis aries (Sheep) Colius colius (White-backed mousebird) Herpetotheres cachinnans (Laughing falcon) (Falco cachinnans) Falco mexicanus (Prairie falcon) Tyrannus tyrannus (Eastern kingbird) And more	434	UniRef90_P17182	Cluster: Alpha-enolase	392

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9W7L2	P17183 Q545V3 D3Z2S4 UPI00042C2859 A0A1V4JAU6 A0A2I0MLQ4 UPI00042BE644 A0A226P8C8 A0A226MZQ2 UPI000A1BB32D +1045	Mus musculus (Mouse) Chelonia mydas (Green sea-turtle) (Chelonia agassizi) Patagioenas fasciata monilis Columba livia (Rock dove) Colinus virginianus (Northern bobwhite) (Tetrao virginianus) Callipepla squamata (Scaled quail) Odocoileus virginianus texanus Rhinolophus sinicus (Chinese rufous horseshoe bat) Chrysemys picta bellii (Western painted turtle) (Emys bellii) Terrapene mexicana triunguis (Three-toed box turtle) And more	434	UniRef50_P17183	Cluster: Gamma-enolase	1,056

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF072587 mRNA Translation: AAD41644.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF072587 mRNA Translation: AAD41644.1

3D structure databases

ProteinModelPortalⁱ	Q9W7L2
SMRⁱ	Q9W7L2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	Q9W7L2

PRIDEⁱ

Q9W7L2

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

HOVERGENⁱ	HBG000067

HOVERGENⁱ

HBG000067

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187

UniPathwayⁱ

UPA00109; UER00187

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.1201 hit 3.30.390.101 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENOA_SCEUN
Accessionⁱ	Q9W7L2Primary (citable) accession number: Q9W7L2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
	Last sequence update:	January 23, 2007
	Last modified:	February 28, 2018
	This is version 82 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - Q9W7L2 (ENOA_SCEUN)

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

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Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

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Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ