Reviewed, UniProtKB/Swiss-Prot Q9W7L2 (ENOA_SCEUN)

Names and origin

Protein names	Recommended name:     Alpha-enolase     EC=4.2.1.11 Alternative name(s):     2-phospho-D-glycerate hydro-lyase     Phosphopyruvate hydratase
Organism	Sceloporus undulatus (Eastern fence lizard) (Skink)
Taxonomic identifier	8520 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Iguania › Iguanidae › Phrynosomatinae › Sceloporus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 434	433	Alpha-enolase		PRO_0000134104
Regions
	Region	370 – 373	4	Substrate binding By similarity
Sites
	Active site	210	1	Proton donor By similarity
	Active site	343	1	Proton acceptor By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	293	1	Magnesium By similarity
	Metal binding	318	1	Magnesium By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Binding site	394	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9W7L2-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6E377E0F0A767E11	FASTA	434	47,493
	10 20 30 40 50 60 MSILKLHARE IFDSRGNPTV EVDLYTNKGL FRAAVPSGAS TGIYEALELR DNDKTRFLGK 70 80 90 100 110 120 GVSKAVEHVN KTIAPALVNK KVNVVEQEKI DKLMLEMDGT ENKSKFGANA ILGVSLAVCK 130 140 150 160 170 180 AGAAEKGVPL YRHIADLAGN EDVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAENFKE 190 200 210 220 230 240 AMRIGAEVYQ NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKSA ISKAGYTDKI 250 260 270 280 290 300 VIGMDVAASE FYRDGKYDLD FKSPDDPSRY ITPDQLSDLY KGFIKNYPVV SIEDPFDQHD 310 320 330 340 350 360 WAAWKKFTAS AGIQVVGDDL TVTNPRRITK AVEEKSCNCL LLKVNQIGSV TESLQACKLA 370 380 390 400 410 420 QTNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQI LRIEEELGSK 430 GRFAGRNFRN PRVN

References

[1]	"Molecular evidence for a clade of turtles." Mannen H., Li S.S.-L. Mol. Phylogenet. Evol. 13:144-148(1999) [PubMed: 10508547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072587 mRNA. Translation: AAD41644.1.
3D structure databases
SMR	Q9W7L2. Positions 2-431.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9W7L2.
Enzyme and pathway databases
BRENDA	4.2.1.11. 278199.
Family and domain databases
InterPro	IPR000941. Enolase. IPR020810. Enolase_C. IPR020809. Enolase_CS. IPR020811. Enolase_N. [Graphical view]
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENOA_SCEUN

Accession

Primary (citable) accession number: Q9W7L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)