Reviewed, UniProtKB/Swiss-Prot Q9W7L1 (ENOA_TRASC)

Names and origin

Protein names	Recommended name:     Alpha-enolase     EC=4.2.1.11 Alternative name(s):     2-phospho-D-glycerate hydro-lyase     Phosphopyruvate hydratase
Organism	Trachemys scripta (Red-eared slider turtle) (Pseudemys scripta)
Taxonomic identifier	34903 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Testudines › Cryptodira › Testudinoidea › Emydidae › Trachemys

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 434	433	Alpha-enolase		PRO_0000134105
Regions
	Region	370 – 373	4	Substrate binding By similarity
Sites
	Active site	210	1	Proton donor By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	293	1	Magnesium By similarity
	Metal binding	318	1	Magnesium By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Binding site	394	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9W7L1-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1AF6D392C539DD16	FASTA	434	47,333
	10 20 30 40 50 60 MSILKIFARE IFDSRGNPTV EVDLYTSKGL FRAAVPSGAS TGIYEALELR DNDKTRFLGK 70 80 90 100 110 120 GVSKAVGHVN KTIAPALISK NINVVEQEKI DKLMLEMDGS ENKSKFGANA ILGVSLAVCK 130 140 150 160 170 180 AGAAEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFKE 190 200 210 220 230 240 AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILED KEALELLKTA ISKAGYTDKV 250 260 270 280 290 300 VIGMDVAASE FYRDGKYDLD FKSPDDPSRY ITPDQLADLY KSFIKSYPLV SVEDPFDQDD 310 320 330 340 350 360 WSAWKKFTAT VGVQVVGDDL TVTNPKRIAK PVEEKSCNCL LLQVNQIGSV TESLQACKLA 370 380 390 400 410 420 QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK 430 ARFAGRNFRN PRIN

References

[1]	"Molecular evidence for a clade of turtles." Mannen H., Li S.S.-L. Mol. Phylogenet. Evol. 13:144-148(1999) [PubMed: 10508547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Subsp. elegans. Tissue: Muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF072588 mRNA. Translation: AAD41645.1.
3D structure databases
HSSP	HSSP built from PDB template 1PDZ based on UniProtKB P56252.
SMR	Q9W7L1. Positions 2-433.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9W7L1.
Enzyme and pathway databases
BRENDA	4.2.1.11. 280693.
Family and domain databases
InterPro	IPR000941. Enolase. [Graphical view]
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
ProDom	PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENOA_TRASC

Accession

Primary (citable) accession number: Q9W7L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 51 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)