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Reviewed, UniProtKB/Swiss-Prot Q9W7K5 (LDHA_CYPCA)

Last modified June 16, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Gene names
Name: ldha
OrganismCyprinus carpio (Common carp)
Taxonomic identifier7962 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprinus

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Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processanaerobic glycolysis

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 333332L-lactate dehydrogenase A chain
PRO_0000168434

Regions

Nucleotide binding30 – 5829NAD By similarity

Sites

Active site1941Proton acceptor By similarity
Binding site1001NAD By similarity
Binding site1071Substrate By similarity
Binding site1391NAD or substrate By similarity
Binding site1701Substrate By similarity
Binding site2491Substrate By similarity

Secondary structure

1 ......................................................... 333
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q9W7K5-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 45B15E6943AD4024

FASTA33336,277
        10         20         30         40         50         60 
MASTKEKLIT HVSKEEPAGP TNKVTVVGVG MVGMAAAISI LLKDLTDELA LVDVMEDKLK 

        70         80         90        100        110        120 
GEAMDLQHGS LFLKTHKIVA DKDYSVTANS KVVVVTAGAR QQEGESRLNL VQRNVNIFKF 

       130        140        150        160        170        180 
IIPNIIKYSP NCILLVVSNP VDILTYVAWK LSGLPRNRVI GSGTNLDSAR FRHLMGEKLG 

       190        200        210        220        230        240 
IHPSNCHGWV IGEHGDSSVP VWSGVNVAGV FLQGLNPDMG TDKDKEDWKS VHKMVVDSAY 

       250        260        270        280        290        300 
EVIKLKGYTS WAIGMSAADL CQSILKNLRK CHPVSTLVKG MHGVNEEVFL SVPCILGNSG 

       310        320        330 
LTDVVHMTLK SDEEKQLVKS AETLWGVQKD LTL

« Hide

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References

[1]"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication."
Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

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Cross-references

Sequence databases

AF076528 mRNA. Translation: AAD40736.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1V6AX-ray2.30A/B2-332[»]
ModBaseSearch...

Phylogenomic databases

HOVERGENQ9W7K5.

Enzyme and pathway databases

BRENDA1.1.1.27. 3298.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLDHA_CYPCA
AccessionPrimary (citable) accession number: Q9W7K5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents