Reviewed, UniProtKB/Swiss-Prot Q9W7J4 (PA21B_PSETE)

Names and origin

Protein names	Recommended name:     Phospholipase A2 1     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase     Pt-PLA1
Organism	Pseudonaja textilis (Eastern brown snake)
Taxonomic identifier	8673 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Pseudonaja

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows moderate PA2 activity and exhibits procoagulant property. Ref.1
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Blood coagulation Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8			PRO_0000022948
	Chain	28 – 154	127	Phospholipase A2 1		PRO_0000022949
Sites
	Active site	75	1	By similarity
	Active site	126	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	58	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 104		By similarity
	Disulfide bond	54 ↔ 153		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 132		By similarity
	Disulfide bond	78 ↔ 125		By similarity
	Disulfide bond	88 ↔ 118		By similarity
	Disulfide bond	111 ↔ 123		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9W7J4-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1A50E3D04492E2C5	FASTA	154	16,844
	10 20 30 40 50 60 MHPAHLLVLL GVCVSLLGAA RIPPLPLSLD DFSNLITCAN RGSRSWLDYA HYGCFCGSGG 70 80 90 100 110 120 SGTPVDDLDR CCQVHDNCFG DAEKLPACNY LFSGPYWNPY SYKCNEGEIT CTDDNDECAA 130 140 150 FICNCDRTAA ICFAGATYND ENFMVTIKKK NICQ

References

[1]

"Group IB phospholipase A2 from Pseudonaja textilis." Armugam A., Gong N.L., Li X.J., Siew P.Y., Chai S.C., Nair R., Jeyaseelan K. Arch. Biochem. Biophys. 421:10-20(2004) [PubMed: 14678780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-39, FUNCTION. Tissue: Venom and Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082983 mRNA. Translation: AAD40975.1. AY027494 Genomic DNA. Translation: AAK15775.1.
3D structure databases
SMR	Q9W7J4. Positions 29-145.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9W7J4.
Enzyme and pathway databases
BRENDA	3.1.1.4. 274617.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_PSETE

Accession

Primary (citable) accession number: Q9W7J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1999
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)