Mothers against decapentaplegic homolog 5

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Q9W7E7 (SMAD5_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mothers against decapentaplegic homolog 5
Short name=MAD homolog 5
Short name=Mothers against DPP homolog 5

Alternative name(s):
Protein somitabun
SMAD family member 5
Short name=SMAD 5
Short name=Smad5

Gene names

Name:	smad5
Synonyms:	madh5, sbn

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in ventralization. May mediate Bmp2b signaling during early phases of embryonic dorsal-ventral pattern formation. Required for initation of Smad1 expression during gastrulation. Ref.1 Ref.4
Subunit structure	May form trimers with the co-SMAD SMAD4 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 By similarity.
Developmental stage	Expressed both maternally and zygotically. Uniform distribution at the cleavage, blastula, gastrula and early somitogenesis stages. Levels decline during the early gastrula stages. At 26 hours, still expressed in the head, forming gut and dorsal neural tube. Ref.1 Ref.2
Sequence similarities	Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	BMP signaling pathway Inferred from mutant phenotype Ref.1. Source: UniProtKB determination of ventral identity Inferred from direct assay. Source: ZFIN dorsal convergence Inferred from mutant phenotype. Source: ZFIN embryonic pattern specification Inferred from mutant phenotype Ref.1 Ref.4. Source: UniProtKB heart looping Inferred from mutant phenotype. Source: ZFIN otic placode formation Inferred from expression pattern. Source: ZFIN regulation of BMP signaling pathway involved in heart jogging Inferred from mutant phenotype. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 464

464

Mothers against decapentaplegic homolog 5

PRO_0000090868

Regions

Domain

13 – 137

125

MH1

Domain

270 – 464

195

MH2

Compositional bias

40 – 46

Poly-Lys

Compositional bias

187 – 231

Pro-rich

Natural variations

Natural variant

429

T → I in allele DTC24; dorsalized embryos. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9W7E7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 5018387FBA4072DC
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FASTA

464

51,861

        10         20         30         40         50         60 
MTSMSSLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME DLEKALSSPG 

        70         80         90        100        110        120 
QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLEVC EYPFGSKQKE 

       130        140        150        160        170        180 
VCINPYHYKR VESPVLPPVL VPRHSEFNPQ HSLLVQFRNL SHNEPHMPLN ATFPESFQQH 

       190        200        210        220        230        240 
SGGSSFPISP NSPYPPSPAS SGTYPNSPAS SGPSSPFQLP ADTPPPAYMP PDEQMGQDGS 

       250        260        270        280        290        300 
QSMETGSSLA PQNMPRGDVQ PVEYQEPSHW CSIVYYELNN RVGEAYHASS TSVLVDGFTD 

       310        320        330        340        350        360 
PSNNKNRFCL GLLSNVNRNS TIENTRRHIG KGVHLYYVGG EVYAECLSDT SIFVQSRNCN 

       370        380        390        400        410        420 
YHHGFHPTTV CKIPSGCSLK IFNNQEFAQL LAQSVNHGFE AVYELTKMCT IRMSFVKGWG 

       430        440        450        460 
AEYHRQDVTS TPCWIEVHLH GPLQWLDKVL TQMGSPLNPI SSVS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The smad5 mutation somitabun blocks Bmp2b signaling during early dorsoventral patterning of the zebrafish embryo." Hild M., Dick A., Rauch G.J., Meier A., Bouwmeester T., Haffter P., Hammerschmidt M. Development 126:2149-2159(1999) [PubMed: 10207140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-429, FUNCTION, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"Characterization of zebrafish smad1, smad2 and smad5: the amino-terminus of Smad1 and Smad5 is required for specific function in the embryo." Mueller F., Blader P., Rastegar S., Fischer N., Knoechel W., Straehle U. Mech. Dev. 88:73-88(1999) [PubMed: 10525190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Embryo.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[4]	"Smad1 and smad5 have distinct roles during dorsoventral patterning of the zebrafish embryo." Dick A., Meier A., Hammerschmidt M. Dev. Dyn. 216:285-298(1999) [PubMed: 10590480] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF127920 mRNA. Translation: AAD43904.1. AF168775 mRNA. Translation: AAF06738.1. BC065644 mRNA. Translation: AAH65644.1.
IPI	IPI00482771.
UniGene	Dr.75335.
3D structure databases
ProteinModelPortal	Q9W7E7.
SMR	Q9W7E7. Positions 10-133, 267-464.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9W7E7.
Proteomic databases
PRIDE	Q9W7E7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000054175; ENSDARP00000054174; ENSDARG00000037238. ENSDART00000129079; ENSDARP00000105258; ENSDARG00000037238.
Organism-specific databases
ZFIN	ZDB-GENE-990603-9. smad5.
Phylogenomic databases
GeneTree	ENSGT00600000084186.
HOGENOM	HBG443554.
HOVERGEN	HBG053353.
InParanoid	Q9W7E7.
OMA	KGAMEDL.
OrthoDB	EOG4HDSTH.
PhylomeDB	Q9W7E7.
Gene expression databases
ArrayExpress	Q9W7E7.
Bgee	Q9W7E7.
Family and domain databases
InterPro	IPR013790. Dwarfin. IPR003619. MAD_homology1_Dwarfin-type. IPR013019. MAD_homology_MH1. IPR017855. SMAD_dom-like. IPR001132. SMAD_dom_Dwarfin-type. IPR008984. SMAD_FHA_domain. [Graphical view]
Gene3D	G3DSA:3.90.520.10. MAD_MH1. 1 hit. G3DSA:2.60.200.10. MH2_Dwarfin-type. 1 hit.
PANTHER	PTHR13703. Dwarfin. 1 hit.
Pfam	PF03165. MH1. 1 hit. PF03166. MH2. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. SM00524. DWB. 1 hit. [Graphical view]
SUPFAM	SSF56366. MAD_MH1. 1 hit. SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS51075. MH1. 1 hit. PS51076. MH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMAD5_DANRE

Accession

Primary (citable) accession number: Q9W7E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	November 1, 1999
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents