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Q9W719

- HPRT_CHICK

UniProt

Q9W719 - HPRT_CHICK

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691GMPBy similarity
    Active sitei138 – 1381Proton acceptorBy similarity
    Binding sitei166 – 1661GMPBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 1429GMPBy similarity
    Nucleotide bindingi186 – 1883GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
    3. magnesium ion binding Source: UniProtKB
    4. nucleotide binding Source: UniProtKB-KW
    5. protein homodimerization activity Source: UniProtKB
    6. purine nucleobase binding Source: AgBase

    GO - Biological processi

    1. GMP catabolic process Source: UniProtKB
    2. guanine salvage Source: UniProtKB
    3. hypoxanthine metabolic process Source: UniProtKB
    4. hypoxanthine salvage Source: UniProtKB
    5. IMP metabolic process Source: UniProtKB
    6. IMP salvage Source: UniProtKB-UniPathway
    7. positive regulation of dopamine metabolic process Source: UniProtKB
    8. protein homotetramerization Source: UniProtKB
    9. purine nucleotide biosynthetic process Source: UniProtKB
    10. purine ribonucleoside salvage Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:HPRT1
    Synonyms:HPRT
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: AgBase
    2. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139592Add
    BLAST

    Proteomic databases

    PaxDbiQ9W719.
    PRIDEiQ9W719.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000031748.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9W719.
    SMRiQ9W719. Positions 5-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236521.
    HOVERGENiHBG000242.
    InParanoidiQ9W719.
    KOiK00760.
    PhylomeDBiQ9W719.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9W719-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATHSPCIVI GDDEQGYDLD LFCIPKHYAD DLEKVYIPHG LIMDRTERLA    50
    REIMKGMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD KSIPMTVDFI 100
    RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMKTLLSLL 150
    KQYNPKMVKV ASLLVKRTPR SVGYRPDFVG FEVPDKFVVG YALDYNEYFR 200
    DLNHICVISE TGKQKYKA 218
    Length:218
    Mass (Da):24,609
    Last modified:November 1, 1999 - v1
    Checksum:i2228B84B0B6AF727
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132697 mRNA. Translation: CAB46657.1.
    RefSeqiNP_990179.1. NM_204848.1.
    UniGeneiGga.3545.

    Genome annotation databases

    GeneIDi395653.
    KEGGigga:395653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132697 mRNA. Translation: CAB46657.1 .
    RefSeqi NP_990179.1. NM_204848.1.
    UniGenei Gga.3545.

    3D structure databases

    ProteinModelPortali Q9W719.
    SMRi Q9W719. Positions 5-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000031748.

    Proteomic databases

    PaxDbi Q9W719.
    PRIDEi Q9W719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395653.
    KEGGi gga:395653.

    Organism-specific databases

    CTDi 3251.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236521.
    HOVERGENi HBG000242.
    InParanoidi Q9W719.
    KOi K00760.
    PhylomeDBi Q9W719.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .

    Miscellaneous databases

    NextBioi 20815725.
    PROi Q9W719.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The chicken HPRT gene: a counter selectable marker for the DT40 cell line."
      Fukagawa T., Hayward N., Yang J., Azzalin C., Griffin D., Stewart A.F., Brown W.
      Nucleic Acids Res. 27:1966-1969(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiHPRT_CHICK
    AccessioniPrimary (citable) accession number: Q9W719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3