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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691GMPBy similarity
Active sitei138 – 1381Proton acceptorBy similarity
Binding sitei166 – 1661GMPBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429GMPBy similarity
Nucleotide bindingi186 – 1883GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB
  4. nucleotide binding Source: UniProtKB-KW
  5. protein homodimerization activity Source: UniProtKB
  6. purine nucleobase binding Source: AgBase

GO - Biological processi

  1. GMP catabolic process Source: UniProtKB
  2. guanine salvage Source: UniProtKB
  3. hypoxanthine metabolic process Source: UniProtKB
  4. hypoxanthine salvage Source: UniProtKB
  5. IMP metabolic process Source: UniProtKB
  6. IMP salvage Source: UniProtKB-UniPathway
  7. positive regulation of dopamine metabolic process Source: UniProtKB
  8. protein homotetramerization Source: UniProtKB
  9. purine nucleotide biosynthetic process Source: UniProtKB
  10. purine ribonucleoside salvage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HPRT1
Synonyms:HPRT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: AgBase
  2. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139592Add
BLAST

Proteomic databases

PaxDbiQ9W719.
PRIDEiQ9W719.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031748.

Structurei

3D structure databases

ProteinModelPortaliQ9W719.
SMRiQ9W719. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiQ9W719.
KOiK00760.
PhylomeDBiQ9W719.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W719-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATHSPCIVI GDDEQGYDLD LFCIPKHYAD DLEKVYIPHG LIMDRTERLA
60 70 80 90 100
REIMKGMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD KSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMKTLLSLL
160 170 180 190 200
KQYNPKMVKV ASLLVKRTPR SVGYRPDFVG FEVPDKFVVG YALDYNEYFR
210
DLNHICVISE TGKQKYKA
Length:218
Mass (Da):24,609
Last modified:November 1, 1999 - v1
Checksum:i2228B84B0B6AF727
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132697 mRNA. Translation: CAB46657.1.
RefSeqiNP_990179.1. NM_204848.1.
UniGeneiGga.3545.

Genome annotation databases

GeneIDi395653.
KEGGigga:395653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132697 mRNA. Translation: CAB46657.1.
RefSeqiNP_990179.1. NM_204848.1.
UniGeneiGga.3545.

3D structure databases

ProteinModelPortaliQ9W719.
SMRiQ9W719. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031748.

Proteomic databases

PaxDbiQ9W719.
PRIDEiQ9W719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395653.
KEGGigga:395653.

Organism-specific databases

CTDi3251.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiQ9W719.
KOiK00760.
PhylomeDBiQ9W719.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Miscellaneous databases

NextBioi20815725.
PROiQ9W719.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The chicken HPRT gene: a counter selectable marker for the DT40 cell line."
    Fukagawa T., Hayward N., Yang J., Azzalin C., Griffin D., Stewart A.F., Brown W.
    Nucleic Acids Res. 27:1966-1969(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHPRT_CHICK
AccessioniPrimary (citable) accession number: Q9W719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: February 4, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.