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Protein

Nuclear receptor coactivator 2

Gene

ncoa2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1) (By similarity). Required in a nuclear receptor pathway to suppress expression of dorsal mesoderm genes. May play a role in the positive regulation of the circadian clock (By similarity).By similarity1 Publication

GO - Molecular functioni

  1. histone acetyltransferase activity Source: InterPro
  2. signal transducer activity Source: InterPro
  3. thyroid hormone receptor binding Source: UniProtKB
  4. thyroid hormone receptor coactivator activity Source: UniProtKB

GO - Biological processi

  1. embryo development Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. positive regulation of receptor activity Source: GOC
  4. regulation of transcription, DNA-templated Source: UniProtKB
  5. rhythmic process Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 2
Short name:
NCoA-2
Alternative name(s):
Transcriptional intermediary factor 2
Short name:
xTIF2
Gene namesi
Name:ncoa2
Synonyms:tif2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-864856. ncoa2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15161516Nuclear receptor coactivator 2PRO_0000094405Add
BLAST

Expressioni

Tissue specificityi

Expressed homogeneously during late blastula-early gastrula stages, becoming highly expressed in the notochord in the late gastrula and neurula stages. At hatching, restricted to a few areas including the blood precursor region, prospective pronephros and retina.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9W705.
SMRiQ9W705. Positions 1056-1100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 8058bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini116 – 18065PASPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi628 – 6325LXXLL motif 1
Motifi680 – 6845LXXLL motif 2
Motifi735 – 7395LXXLL motif 3
Motifi1064 – 10729LLXXLXXXL motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1237 – 127337Poly-GlnAdd
BLAST

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and, at least in part, functionally redundant (By similarity).By similarity
The LLXXLXXXL motif is involved in transcriptional coactivation and crebbp/cbp binding.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11255.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028822. NCOA2.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF2. PTHR10684:SF2. 1 hit.
PfamiPF07469. DUF1518. 2 hits.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGENLSDPSR AEPRKRKESL DQLGPSPKRS TEKRNREQEN KYIEELAELI
60 70 80 90 100
FANFNDIDNL NFKPDKCAIL KETVKQIRQI KEHEKTAAAN EDEVQKADVS
110 120 130 140 150
STGQSVIDKD ALGPMMLEAL DGFFFVVNRE GNVVFVSENV TQYLRYNQEE
160 170 180 190 200
LMNTSVYSIL HVGDHSEFIK NLLPKSLVNG VPRRNSHTFN CRMLVKPMME
210 220 230 240 250
CEEERHDGQE THQKYESMQC FAVSQPKSIK EEGEDFQSCL ICVARRVPVK
260 270 280 290 300
ERPVPPPSES FTTRQDFQGK ITSLDTTSMR ALMRPGWEDM VRRCIQRFHS
310 320 330 340 350
QHDGEISYSR RHHQEVLRQG HATSPFYRFS LSDGTTVVAQ TKSRLMRSQT
360 370 380 390 400
NNEPPLVLSL HVLQREQNVC GLNQDLAGQA MGKTLNPVQS SSPAHQAMYG
410 420 430 440 450
GNPGQDTTIS SNMNYAISGP KEQMGLATGR FVGSGGMNHI SSLQATTPQG
460 470 480 490 500
NNYALKMNSP SHGSPGMGQG QPNSMLSPRH RVSPGVAGSP RIAPSPFSPA
510 520 530 540 550
GSLHSPVSVC SSTGNSHSYT NSSLNALQAL SEGQGPLAPP LSSPDLKGGN
560 570 580 590 600
LQHSPGNMNP PQLRKMGSID SKESFGLYEE QPESATGQGE SGCHSNEQKD
610 620 630 640 650
CGENLSSVVD KTEGQSRLLD GKGQQKLLKL LTTKSDQMEP STLPSNTLGD
660 670 680 690 700
MNKDSLSNFA SNSMSASAHG TSLKEKHKIL HRLLQDSSSP VDLAKLTAEA
710 720 730 740 750
TGKELSQESN STGPGSEVTI KQEPVSPKKK EHALLRYLLD KDDTTDNVAD
760 770 780 790 800
ITPKLERADN KVDPSSCPKL SAVKAEKEEP NFGHTDQPGS DFDNLDEILD
810 820 830 840 850
DLQNSQLSQL FSDTRHDGNS ADKQAIMNDL MQLAGENSTG LPAWAQKQRM
860 870 880 890 900
LRMQQNNGFN SQLAAQLGRL PNQNLPLDIH FQSQASAGSF AQMRSSGPYT
910 920 930 940 950
TVPQSGVINN QAMMGSQGNV RNSSPGIVGV NGPRPPLKPG DWGSQASAVR
960 970 980 990 1000
PACPTTSTAM NRHDMSRSPT ASIPMRPGSQ VCPRQVLQSA VMNMGSSELD
1010 1020 1030 1040 1050
MNISGPQYTQ QQAPPNQTAP WPNRILTIEQ PSFNNQNRQP FGSPADDLIC
1060 1070 1080 1090 1100
QPIVSESPAD DGNLLDQLYM ALRNFDGLEE IDRALGIPEM VSQGQAVEQE
1110 1120 1130 1140 1150
SFGSPESNLM MEQKPPVYNH AYANQGQMAQ NSYQPMQDPG FNPMGQRPSY
1160 1170 1180 1190 1200
GILRMQNRPG LRPTGMVQNQ PNQLRLQLQH RLQAQNRQQL MNPINNVSNM
1210 1220 1230 1240 1250
NLAMRPGVPG QLREQGPINA QMLAQRQREI LSQHLRQKQL QQQQQQQQQQ
1260 1270 1280 1290 1300
QQQQQQQQQQ QQQQQQQQQQ QQQHRAMMMR SQGLAMPPNM VGSGGIPASI
1310 1320 1330 1340 1350
NSPRIPQGST QQFPFPPNYG TGIPSPPPFT SPFSPVPPSP GSQSLSHSSL
1360 1370 1380 1390 1400
HGSQMNLANQ GIMGSMGGQY GPVMNPQMQH NAFQFANSGM SQQSDPGFTG
1410 1420 1430 1440 1450
ATTPQSPIMS PRMGHIQSSM MQQSQANPAY QSELNGWAQG NPAGNSMFSQ
1460 1470 1480 1490 1500
QSPPHFGQQS GTSMYNSNNM NISVSMAANG NGMNNMNQMT GQINMTSVTS
1510
VPTSGLSSMG PEQKYC
Length:1,516
Mass (Da):166,156
Last modified:October 31, 1999 - v1
Checksum:i09851C00AB439A4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243119 mRNA. Translation: CAB45389.1.
RefSeqiNP_001081139.1. NM_001087670.1.
UniGeneiXl.4857.

Genome annotation databases

GeneIDi394405.
KEGGixla:394405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243119 mRNA. Translation: CAB45389.1.
RefSeqiNP_001081139.1. NM_001087670.1.
UniGeneiXl.4857.

3D structure databases

ProteinModelPortaliQ9W705.
SMRiQ9W705. Positions 1056-1100.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi394405.
KEGGixla:394405.

Organism-specific databases

CTDi10499.
XenbaseiXB-GENE-864856. ncoa2.

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11255.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028822. NCOA2.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF2. PTHR10684:SF2. 1 hit.
PfamiPF07469. DUF1518. 2 hits.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "XTIF2, a Xenopus homologue of the human transcription intermediary factor, is required for a nuclear receptor pathway that also interacts with CBP to suppress Brachyury and XMyoD."
    de la Calle-Mustienes E., Gomez-Skarmeta J.L.
    Mech. Dev. 91:119-129(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNCOA2_XENLA
AccessioniPrimary (citable) accession number: Q9W705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2001
Last sequence update: October 31, 1999
Last modified: March 31, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.