Q9W705 (NCOA2_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear receptor coactivator 2
Short name=NCoA-2

Alternative name(s):
Transcriptional intermediary factor 2
Short name=xTIF2

Gene names

Name:	ncoa2
Synonyms:	tif2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	1516 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1) By similarity. Required in a nuclear receptor pathway to suppress expression of dorsal mesoderm genes. Ref.1
Subcellular location	Nucleus.
Tissue specificity	Expressed homogeneously during late blastula-early gastrula stages, becoming highly expressed in the notochord in the late gastrula and neurula stages. At hatching, restricted to a few areas including the blood precursor region, prospective pronephros and retina. Ref.1
Domain	Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and, at least in part, functionally redundant By similarity. The LLXXLXXXL motif is involved in transcriptional coactivation and crebbp/cbp binding By similarity. Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently By similarity.
Sequence similarities	Belongs to the SRC/p160 nuclear receptor coactivator family. Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Activator Developmental protein
Gene Ontology (GO)
Biological_process	embryo development Inferred from mutant phenotype Ref.1. Source: UniProtKB histone acetylation Inferred from electronic annotation. Source: GOC intracellular signal transduction Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	histone acetyltransferase activity Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro thyroid hormone receptor binding Inferred from sequence or structural similarity. Source: UniProtKB thyroid hormone receptor coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1516	1516	Nuclear receptor coactivator 2		PRO_0000094405
Regions
	Domain	23 – 80	58	bHLH
	Domain	116 – 180	65	PAS
	Motif	628 – 632	5	LXXLL motif 1
	Motif	680 – 684	5	LXXLL motif 2
	Motif	735 – 739	5	LXXLL motif 3
	Motif	1064 – 1072	9	LLXXLXXXL motif
	Compositional bias	1237 – 1273	37	Poly-Gln

Sequences

	Sequence		Length	Mass (Da)
	Q9W705 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 09851C00AB439A4A	FASTA	1,516	166,156
	10 20 30 40 50 60 MGENLSDPSR AEPRKRKESL DQLGPSPKRS TEKRNREQEN KYIEELAELI FANFNDIDNL 70 80 90 100 110 120 NFKPDKCAIL KETVKQIRQI KEHEKTAAAN EDEVQKADVS STGQSVIDKD ALGPMMLEAL 130 140 150 160 170 180 DGFFFVVNRE GNVVFVSENV TQYLRYNQEE LMNTSVYSIL HVGDHSEFIK NLLPKSLVNG 190 200 210 220 230 240 VPRRNSHTFN CRMLVKPMME CEEERHDGQE THQKYESMQC FAVSQPKSIK EEGEDFQSCL 250 260 270 280 290 300 ICVARRVPVK ERPVPPPSES FTTRQDFQGK ITSLDTTSMR ALMRPGWEDM VRRCIQRFHS 310 320 330 340 350 360 QHDGEISYSR RHHQEVLRQG HATSPFYRFS LSDGTTVVAQ TKSRLMRSQT NNEPPLVLSL 370 380 390 400 410 420 HVLQREQNVC GLNQDLAGQA MGKTLNPVQS SSPAHQAMYG GNPGQDTTIS SNMNYAISGP 430 440 450 460 470 480 KEQMGLATGR FVGSGGMNHI SSLQATTPQG NNYALKMNSP SHGSPGMGQG QPNSMLSPRH 490 500 510 520 530 540 RVSPGVAGSP RIAPSPFSPA GSLHSPVSVC SSTGNSHSYT NSSLNALQAL SEGQGPLAPP 550 560 570 580 590 600 LSSPDLKGGN LQHSPGNMNP PQLRKMGSID SKESFGLYEE QPESATGQGE SGCHSNEQKD 610 620 630 640 650 660 CGENLSSVVD KTEGQSRLLD GKGQQKLLKL LTTKSDQMEP STLPSNTLGD MNKDSLSNFA 670 680 690 700 710 720 SNSMSASAHG TSLKEKHKIL HRLLQDSSSP VDLAKLTAEA TGKELSQESN STGPGSEVTI 730 740 750 760 770 780 KQEPVSPKKK EHALLRYLLD KDDTTDNVAD ITPKLERADN KVDPSSCPKL SAVKAEKEEP 790 800 810 820 830 840 NFGHTDQPGS DFDNLDEILD DLQNSQLSQL FSDTRHDGNS ADKQAIMNDL MQLAGENSTG 850 860 870 880 890 900 LPAWAQKQRM LRMQQNNGFN SQLAAQLGRL PNQNLPLDIH FQSQASAGSF AQMRSSGPYT 910 920 930 940 950 960 TVPQSGVINN QAMMGSQGNV RNSSPGIVGV NGPRPPLKPG DWGSQASAVR PACPTTSTAM 970 980 990 1000 1010 1020 NRHDMSRSPT ASIPMRPGSQ VCPRQVLQSA VMNMGSSELD MNISGPQYTQ QQAPPNQTAP 1030 1040 1050 1060 1070 1080 WPNRILTIEQ PSFNNQNRQP FGSPADDLIC QPIVSESPAD DGNLLDQLYM ALRNFDGLEE 1090 1100 1110 1120 1130 1140 IDRALGIPEM VSQGQAVEQE SFGSPESNLM MEQKPPVYNH AYANQGQMAQ NSYQPMQDPG 1150 1160 1170 1180 1190 1200 FNPMGQRPSY GILRMQNRPG LRPTGMVQNQ PNQLRLQLQH RLQAQNRQQL MNPINNVSNM 1210 1220 1230 1240 1250 1260 NLAMRPGVPG QLREQGPINA QMLAQRQREI LSQHLRQKQL QQQQQQQQQQ QQQQQQQQQQ 1270 1280 1290 1300 1310 1320 QQQQQQQQQQ QQQHRAMMMR SQGLAMPPNM VGSGGIPASI NSPRIPQGST QQFPFPPNYG 1330 1340 1350 1360 1370 1380 TGIPSPPPFT SPFSPVPPSP GSQSLSHSSL HGSQMNLANQ GIMGSMGGQY GPVMNPQMQH 1390 1400 1410 1420 1430 1440 NAFQFANSGM SQQSDPGFTG ATTPQSPIMS PRMGHIQSSM MQQSQANPAY QSELNGWAQG 1450 1460 1470 1480 1490 1500 NPAGNSMFSQ QSPPHFGQQS GTSMYNSNNM NISVSMAANG NGMNNMNQMT GQINMTSVTS 1510 VPTSGLSSMG PEQKYC

References

[1]

"XTIF2, a Xenopus homologue of the human transcription intermediary factor, is required for a nuclear receptor pathway that also interacts with CBP to suppress Brachyury and XMyoD." de la Calle-Mustienes E., Gomez-Skarmeta J.L. Mech. Dev. 91:119-129(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ243119 mRNA. Translation: CAB45389.1.
RefSeq	NP_001081139.1. NM_001087670.1.
UniGene	Xl.4857.
3D structure databases
ProteinModelPortal	Q9W705.
SMR	Q9W705. Positions 1056-1100.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	394405.
KEGG	xla:394405.
Organism-specific databases
CTD	10499.
Xenbase	XB-GENE-864856. ncoa2.
Phylogenomic databases
HOVERGEN	HBG052583.
KO	K11255.
Family and domain databases
Gene3D	4.10.280.10. 1 hit. 4.10.630.10. 2 hits.
InterPro	IPR011598. bHLH_dom. IPR010011. DUF1518. IPR009110. Nuc_rcpt_coact. IPR014920. Nuc_rcpt_coact_Ncoa-typ. IPR017426. Nuclear_rcpt_coactivator. IPR000014. PAS. IPR013767. PAS_fold. IPR014935. SRC-1. IPR008955. Src1_rcpt_coact. [Graphical view]
Pfam	PF07469. DUF1518. 2 hits. PF08815. Nuc_rec_co-act. 1 hit. PF00989. PAS. 1 hit. PF08832. SRC-1. 1 hit. [Graphical view]
PIRSF	PIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMART	SM00353. HLH. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit. SSF69125. Nuc_recept_coact. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCOA2_XENLA

Accession

Primary (citable) accession number: Q9W705

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program