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Protein

Pituitary homeobox 2

Gene

pitx2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Isoform a and isoform c differentially regulate mesendoderm formation and asymmetric organ development. Both isoforms regulate left-right development, but isoform a has a stronger effect on heart laterality.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi82 – 141HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • camera-type eye morphogenesis Source: ZFIN
  • closure of optic fissure Source: ZFIN
  • determination of digestive tract left/right asymmetry Source: ZFIN
  • determination of left/right asymmetry in diencephalon Source: ZFIN
  • embryonic camera-type eye morphogenesis Source: ZFIN
  • embryonic cranial skeleton morphogenesis Source: ZFIN
  • embryonic heart tube left/right pattern formation Source: ZFIN
  • embryonic viscerocranium morphogenesis Source: ZFIN
  • epithalamus development Source: ZFIN
  • face morphogenesis Source: ZFIN
  • habenula development Source: ZFIN
  • mesendoderm development Source: ZFIN
  • prechordal plate formation Source: ZFIN
  • regulation of vascular permeability Source: ZFIN
  • retinoic acid receptor signaling pathway Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 2
Alternative name(s):
Homeobox protein PITX2
Paired-like homeodomain transcription factor 2
Gene namesi
Name:pitx2
Synonyms:pitx2a, pitx2b
ORF Names:zgc:110508
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 14

Organism-specific databases

ZFINiZDB-GENE-990714-27. pitx2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492271 – 314Pituitary homeobox 2Add BLAST314

Proteomic databases

PaxDbiQ9W5Z2.

Expressioni

Tissue specificityi

Isoform a and isoform c have distinct expression patterns during early mesendoderm development; isoform c is expressed symmetrically in presumptive mesendoderm during late blastula stages, and in both the anterior (polster) and posterior prechordal plate during late gastrulation. In contrast, isoform a expression begins at bud stage, in the anterior prechordal plate (polster). At 4- to 6-somite stages, isoform a and isoform c are both expressed in the polster, and weak isoform c expression remains in the posterior prechordal plate. Isoform a is also expressed in lateral head mesendoderm. Isoform a and isoform c are also expressed in different asymmetric domains during development of the diencephalon, gut and heart; isoform c is restricted to the dorsal diencephalon and left hand side of the developing gut, whereas isoform a shows left-sided asymmetric expression in the developing heart, with strongest expression in ther anterior regions.1 Publication

Developmental stagei

Isoform c is expressed during dome stage, while isoform a is not expressed until bud stage, following gastrulation. Isoform a shows strong expression during heart looping stages (30-36 hpf) and at 48 hpf.1 Publication

Inductioni

Isoform c is regulated by tgf-beta signaling in the early mesendoderm.1 Publication

Gene expression databases

BgeeiENSDARG00000036194.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000052568.

Structurei

3D structure databases

ProteinModelPortaliQ9W5Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 289OARPROSITE-ProRule annotationAdd BLAST14
Motifi282 – 286Nuclear localization signalSequence analysis5

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0486. Eukaryota.
ENOG410ZZ6J. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiQ9W5Z2.
KOiK04686.
OMAiPLNIDHH.
OrthoDBiEOG091G0H7U.
PhylomeDBiQ9W5Z2.
TreeFamiTF351940.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform c (identifier: Q9W5Z2-1) [UniParc]FASTAAdd to basket
Also known as: Pitx2c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSMKDPLSL DHHHHNHHVT GSKHAPLSMA SSLQPLQRSV DSKHRLDVHT
60 70 80 90 100
VSDTSSPESV EKEKGQSKNE DSNDDPSKKK RQRRQRTHFT SQQLQELEAT
110 120 130 140 150
FQRNRYPDMS TREEIAVWTN LTEARVRVWF KNRRAKWRKR ERNQQAELCK
160 170 180 190 200
NGFGPQFNGL MQPYDDMYPS YTYNNWAAKG LTSASLSTKS FPFFNSMNVN
210 220 230 240 250
PLSSQTMFSP PNSISSMSMS SSMVPSAVTG VPGSSLNSLN NLNNLSNPSL
260 270 280 290 300
NSGVPTPACP YAPPTPPYVY RDTCNSSLAS LRLKAKQHSS FGYASVQNPA
310
SNLSACQYAV DRPV
Length:314
Mass (Da):35,187
Last modified:November 1, 1999 - v1
Checksum:i52D0F7855664A6B5
GO
Isoform a (identifier: Q9W5Z2-2) [UniParc]FASTAAdd to basket
Also known as: Pitx2a

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
     46-60: LDVHTVSDTSSPESV → MDSHCRKLASTCAQL

Show »
Length:269
Mass (Da):30,222
Checksum:i9ACF632FD4C1088F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → T in AAH92922 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0022681 – 45Missing in isoform a. 3 PublicationsAdd BLAST45
Alternative sequenceiVSP_00226946 – 60LDVHT…SPESV → MDSHCRKLASTCAQL in isoform a. 3 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156905 mRNA. Translation: AAD40179.1.
AF156906 mRNA. Translation: AAD40180.1.
AF181681 mRNA. Translation: AAF00486.2.
AF132446 mRNA. Translation: AAD34390.1.
AF132447 mRNA. Translation: AAD34391.1.
BC092922 mRNA. Translation: AAH92922.1.
RefSeqiNP_571050.1. NM_130975.2. [Q9W5Z2-1]
XP_005157364.1. XM_005157307.3. [Q9W5Z2-2]
UniGeneiDr.81292.

Genome annotation databases

EnsembliENSDART00000052569; ENSDARP00000052568; ENSDARG00000036194. [Q9W5Z2-1]
ENSDART00000148319; ENSDARP00000120155; ENSDARG00000036194. [Q9W5Z2-2]
GeneIDi30164.
KEGGidre:30164.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156905 mRNA. Translation: AAD40179.1.
AF156906 mRNA. Translation: AAD40180.1.
AF181681 mRNA. Translation: AAF00486.2.
AF132446 mRNA. Translation: AAD34390.1.
AF132447 mRNA. Translation: AAD34391.1.
BC092922 mRNA. Translation: AAH92922.1.
RefSeqiNP_571050.1. NM_130975.2. [Q9W5Z2-1]
XP_005157364.1. XM_005157307.3. [Q9W5Z2-2]
UniGeneiDr.81292.

3D structure databases

ProteinModelPortaliQ9W5Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000052568.

Proteomic databases

PaxDbiQ9W5Z2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000052569; ENSDARP00000052568; ENSDARG00000036194. [Q9W5Z2-1]
ENSDART00000148319; ENSDARP00000120155; ENSDARG00000036194. [Q9W5Z2-2]
GeneIDi30164.
KEGGidre:30164.

Organism-specific databases

CTDi5308.
ZFINiZDB-GENE-990714-27. pitx2.

Phylogenomic databases

eggNOGiKOG0486. Eukaryota.
ENOG410ZZ6J. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiQ9W5Z2.
KOiK04686.
OMAiPLNIDHH.
OrthoDBiEOG091G0H7U.
PhylomeDBiQ9W5Z2.
TreeFamiTF351940.

Miscellaneous databases

PROiQ9W5Z2.

Gene expression databases

BgeeiENSDARG00000036194.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPITX2_DANRE
AccessioniPrimary (citable) accession number: Q9W5Z2
Secondary accession number(s): Q568C4, Q9W5Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: October 5, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.