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Q9W5D4

- POMT2_DROME

UniProt

Q9W5D4 - POMT2_DROME

Protein

Protein O-mannosyl-transferase 2

Gene

tw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Rt/POMT1 and tw/POMT2 function as a protein O-mannosyltransferase in association with each other to generate and maintain normal muscle development.2 Publications

    Catalytic activityi

    Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. lipid glycosylation Source: UniProtKB
    2. muscle attachment Source: FlyBase
    3. muscle organ development Source: UniProtKB
    4. protein O-linked mannosylation Source: FlyBase
    5. sarcomere organization Source: FlyBase
    6. somatic muscle development Source: FlyBase

    Keywords - Molecular functioni

    Developmental protein, Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT39. Glycosyltransferase Family 39.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
    Alternative name(s):
    Dolichyl-phosphate-mannose--protein mannosyltransferase 2
    Short name:
    DmPOMT2
    Short name:
    dPOMT2
    Protein twisted
    Gene namesi
    Name:tw
    Synonyms:Pomt2
    ORF Names:CG12311
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0086368. tw.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: FlyBase

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Death during development, the few adult escapers exhibit clockwise rotation of the abdomen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 765765Protein O-mannosyl-transferase 2PRO_0000121490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi751 – 7511N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9W5D4.
    PRIDEiQ9W5D4.

    Expressioni

    Tissue specificityi

    At the cellular blastoderm stage, expression accumulates in the ventrally located mesoderm primordium. At germ band extension, mesoderm expression is seen as stripes of strong expression. A very strong signal is also detected in the invaginating gut. As the germ band retracts, mesodermal expression decays and becomes restricted to somatic muscle precursors.1 Publication

    Developmental stagei

    Expressed throughout development; expression peaks at embryonic stages 8-16.2 Publications

    Gene expression databases

    BgeeiQ9W5D4.

    Interactioni

    Subunit structurei

    Interacts with Rt/POMT1.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ9W5D4.
    SMRiQ9W5D4. Positions 320-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei128 – 14821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei268 – 28821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei566 – 58621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei667 – 68721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei689 – 70921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei719 – 73921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini318 – 37457MIR 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini384 – 44057MIR 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini445 – 50157MIR 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 39 family.Sequence Analysis
    Contains 3 MIR domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1928.
    GeneTreeiENSGT00740000115531.
    InParanoidiQ9W5D4.
    KOiK00728.
    OMAiWLCISRF.
    OrthoDBiEOG79KPDP.
    PhylomeDBiQ9W5D4.

    Family and domain databases

    InterProiIPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view]
    PANTHERiPTHR10050. PTHR10050. 1 hit.
    PfamiPF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view]
    SMARTiSM00472. MIR. 3 hits.
    [Graphical view]
    SUPFAMiSSF82109. SSF82109. 1 hit.
    PROSITEiPS50919. MIR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9W5D4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASVVKTPK CPRRGSVKDV AQNAPRTAPT SSKEANWNWW LLLATVFLVT    50
    FATRFYKVTE PDHICWDETH FGKMGSWYIN RTFFFDVHPP LGKMLIGLSG 100
    YLTGYNGTFP FEKPGDKYNE TRYQGMRYFC TTLGALIMPM GFDTVYDLTR 150
    SHEAALLAAA YLIFDVGLLT LNQYILLDPI LLFFMMASVW GMVKVSKSTA 200
    SGGSYGLRWW LWLFLTGTML SCTISVKFVG LFVVLLVGLH TATELWLILG 250
    DLGQPILETV KQLACRAITL IVWPVLLYIL FFYIHLSVLN RSGNGDGFYS 300
    SAFQSRLIGN SLYNASMPRD VAYGSLVTIK NHKTGGGYLH SHHHLYPKGS 350
    GARQQQVTTY THKDENNKWL IRPHNKPGPP KGKVQILRHG DLVRLTHMAT 400
    RRNLHSHNEP APMTKKHLQV TGYGELGLGD ANDVWRVLIV GGKVNETVHT 450
    VTSRLKFIHL LQNCALTSSG KQLPKWGFEQ QEVSCNPNVR DKNSQWNVED 500
    NEHKLMPSVS FSVYAPGFFA RFLESHAVML QGNAGLKPKE GEVTSRPWQW 550
    PINYRGQFFS GSSYRIYLLG NPLIWWSNLV FLALFVTVFL CNAVVQQRRA 600
    GFARSAAQNQ AQVPDSETVA QDEESEHSTT DICSCCTPAK EIVPKAVPSG 650
    SPEAPNPAQS LRAAAWLFLG WMLHYLPFWA MGRVLYFHHY FPALIFNSLL 700
    TGVMYNYILR VLPKWIHHVI LGLVLSILVY SFAAFSPLAY GMSGPLANEP 750
    NSTMYNLKWL STWEF 765
    Length:765
    Mass (Da):86,250
    Last modified:May 1, 2000 - v1
    Checksum:i250CC8486330CB94
    GO

    Sequence cautioni

    The sequence CAA20897.1 differs from that shown. Reason: Erroneous gene model prediction.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti59 – 591T → GS in allele tw[1].

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB176551 mRNA. Translation: BAD54755.1.
    AE014298 Genomic DNA. Translation: AAF45548.1.
    AL031583 Genomic DNA. Translation: CAA20897.1. Sequence problems.
    AY095525 mRNA. Translation: AAM12256.1.
    PIRiT13483.
    RefSeqiNP_001259102.1. NM_001272173.1.
    NP_569858.1. NM_130502.3.
    UniGeneiDm.12266.

    Genome annotation databases

    EnsemblMetazoaiFBtr0070139; FBpp0070134; FBgn0086368.
    GeneIDi31024.
    KEGGidme:Dmel_CG12311.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB176551 mRNA. Translation: BAD54755.1 .
    AE014298 Genomic DNA. Translation: AAF45548.1 .
    AL031583 Genomic DNA. Translation: CAA20897.1 . Sequence problems.
    AY095525 mRNA. Translation: AAM12256.1 .
    PIRi T13483.
    RefSeqi NP_001259102.1. NM_001272173.1.
    NP_569858.1. NM_130502.3.
    UniGenei Dm.12266.

    3D structure databases

    ProteinModelPortali Q9W5D4.
    SMRi Q9W5D4. Positions 320-496.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT39. Glycosyltransferase Family 39.

    Proteomic databases

    PaxDbi Q9W5D4.
    PRIDEi Q9W5D4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0070139 ; FBpp0070134 ; FBgn0086368 .
    GeneIDi 31024.
    KEGGi dme:Dmel_CG12311.

    Organism-specific databases

    CTDi 8168.
    FlyBasei FBgn0086368. tw.

    Phylogenomic databases

    eggNOGi COG1928.
    GeneTreei ENSGT00740000115531.
    InParanoidi Q9W5D4.
    KOi K00728.
    OMAi WLCISRF.
    OrthoDBi EOG79KPDP.
    PhylomeDBi Q9W5D4.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    GenomeRNAii 31024.
    NextBioi 771564.
    PROi Q9W5D4.

    Gene expression databases

    Bgeei Q9W5D4.

    Family and domain databases

    InterProi IPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view ]
    PANTHERi PTHR10050. PTHR10050. 1 hit.
    Pfami PF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view ]
    SMARTi SM00472. MIR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF82109. SSF82109. 1 hit.
    PROSITEi PS50919. MIR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity."
      Ichimiya T., Manya H., Ohmae Y., Yoshida H., Takahashi K., Ueda R., Endo T., Nishihara S.
      J. Biol. Chem. 279:42638-42647(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    2. "The twisted gene encodes Drosophila protein O-mannosyltransferase 2 and genetically interacts with the rotated abdomen gene encoding Drosophila protein O-mannosyltransferase 1."
      Lyalin D., Koles K., Roosendaal S.D., Repnikova E., Van Wechel L., Panin V.M.
      Genetics 172:343-353(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE TW[1]), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Oregon-R1 Publication.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BerkeleyImported.
      Tissue: Larva1 Publication and Pupae1 Publication.

    Entry informationi

    Entry nameiPOMT2_DROME
    AccessioniPrimary (citable) accession number: Q9W5D4
    Secondary accession number(s): O77437
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3