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Q9W5D4 (POMT2_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein O-mannosyl-transferase 2

EC=2.4.1.109
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Short name=DmPOMT2
Short name=dPOMT2
Protein twisted
Gene names
Name:tw
Synonyms:Pomt2
ORF Names:CG12311
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Rt/POMT1 and tw/POMT2 function as a protein O-mannosyltransferase in association with each other to generate and maintain normal muscle development. Ref.1 Ref.2

Catalytic activity

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein. Ref.1

Pathway

Protein modification; protein glycosylation.

Subunit structure

Interacts with Rt/POMT1 Probable.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

At the cellular blastoderm stage, expression accumulates in the ventrally located mesoderm primordium. At germ band extension, mesoderm expression is seen as stripes of strong expression. A very strong signal is also detected in the invaginating gut. As the germ band retracts, mesodermal expression decays and becomes restricted to somatic muscle precursors. Ref.1

Developmental stage

Expressed throughout development; expression peaks at embryonic stages 8-16. Ref.1 Ref.2

Disruption phenotype

Death during development, the few adult escapers exhibit clockwise rotation of the abdomen. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Sequence caution

The sequence CAA20897.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 765765Protein O-mannosyl-transferase 2
PRO_0000121490

Regions

Transmembrane35 – 5521Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane206 – 22621Helical; Potential
Transmembrane228 – 24821Helical; Potential
Transmembrane268 – 28821Helical; Potential
Transmembrane566 – 58621Helical; Potential
Transmembrane667 – 68721Helical; Potential
Transmembrane689 – 70921Helical; Potential
Transmembrane719 – 73921Helical; Potential
Domain318 – 37457MIR 1
Domain384 – 44057MIR 2
Domain445 – 50157MIR 3

Amino acid modifications

Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential
Glycosylation2901N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation7511N-linked (GlcNAc...) Potential

Natural variations

Natural variant591T → GS in allele tw[1].

Sequences

Sequence LengthMass (Da)Tools
Q9W5D4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 250CC8486330CB94

FASTA76586,250
        10         20         30         40         50         60 
MAASVVKTPK CPRRGSVKDV AQNAPRTAPT SSKEANWNWW LLLATVFLVT FATRFYKVTE 

        70         80         90        100        110        120 
PDHICWDETH FGKMGSWYIN RTFFFDVHPP LGKMLIGLSG YLTGYNGTFP FEKPGDKYNE 

       130        140        150        160        170        180 
TRYQGMRYFC TTLGALIMPM GFDTVYDLTR SHEAALLAAA YLIFDVGLLT LNQYILLDPI 

       190        200        210        220        230        240 
LLFFMMASVW GMVKVSKSTA SGGSYGLRWW LWLFLTGTML SCTISVKFVG LFVVLLVGLH 

       250        260        270        280        290        300 
TATELWLILG DLGQPILETV KQLACRAITL IVWPVLLYIL FFYIHLSVLN RSGNGDGFYS 

       310        320        330        340        350        360 
SAFQSRLIGN SLYNASMPRD VAYGSLVTIK NHKTGGGYLH SHHHLYPKGS GARQQQVTTY 

       370        380        390        400        410        420 
THKDENNKWL IRPHNKPGPP KGKVQILRHG DLVRLTHMAT RRNLHSHNEP APMTKKHLQV 

       430        440        450        460        470        480 
TGYGELGLGD ANDVWRVLIV GGKVNETVHT VTSRLKFIHL LQNCALTSSG KQLPKWGFEQ 

       490        500        510        520        530        540 
QEVSCNPNVR DKNSQWNVED NEHKLMPSVS FSVYAPGFFA RFLESHAVML QGNAGLKPKE 

       550        560        570        580        590        600 
GEVTSRPWQW PINYRGQFFS GSSYRIYLLG NPLIWWSNLV FLALFVTVFL CNAVVQQRRA 

       610        620        630        640        650        660 
GFARSAAQNQ AQVPDSETVA QDEESEHSTT DICSCCTPAK EIVPKAVPSG SPEAPNPAQS 

       670        680        690        700        710        720 
LRAAAWLFLG WMLHYLPFWA MGRVLYFHHY FPALIFNSLL TGVMYNYILR VLPKWIHHVI 

       730        740        750        760 
LGLVLSILVY SFAAFSPLAY GMSGPLANEP NSTMYNLKWL STWEF 

« Hide

References

« Hide 'large scale' references
[1]"The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity."
Ichimiya T., Manya H., Ohmae Y., Yoshida H., Takahashi K., Ueda R., Endo T., Nishihara S.
J. Biol. Chem. 279:42638-42647(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]"The twisted gene encodes Drosophila protein O-mannosyltransferase 2 and genetically interacts with the rotated abdomen gene encoding Drosophila protein O-mannosyltransferase 1."
Lyalin D., Koles K., Roosendaal S.D., Repnikova E., Van Wechel L., Panin V.M.
Genetics 172:343-353(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE TW[1]), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]"From sequence to chromosome: the tip of the X chromosome of D. melanogaster."
Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. expand/collapse author list , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M.
Science 287:2220-2222(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Oregon-R.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Larva and Pupae.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB176551 mRNA. Translation: BAD54755.1.
AE014298 Genomic DNA. Translation: AAF45548.1.
AL031583 Genomic DNA. Translation: CAA20897.1. Sequence problems.
AY095525 mRNA. Translation: AAM12256.1.
PIRT13483.
RefSeqNP_001259102.1. NM_001272173.1.
NP_569858.1. NM_130502.3.
UniGeneDm.12266.

3D structure databases

ProteinModelPortalQ9W5D4.
SMRQ9W5D4. Positions 320-502.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT39. Glycosyltransferase Family 39.

Proteomic databases

PaxDbQ9W5D4.
PRIDEQ9W5D4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0070139; FBpp0070134; FBgn0086368.
GeneID31024.
KEGGdme:Dmel_CG12311.

Organism-specific databases

CTD8168.
FlyBaseFBgn0086368. tw.

Phylogenomic databases

eggNOGCOG1928.
GeneTreeENSGT00740000115531.
InParanoidQ9W5D4.
KOK00728.
OMAWLCISRF.
OrthoDBEOG79KPDP.
PhylomeDBQ9W5D4.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ9W5D4.

Family and domain databases

InterProIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERPTHR10050. PTHR10050. 1 hit.
PfamPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMSSF82109. SSF82109. 1 hit.
PROSITEPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi31024.
NextBio771564.
PROQ9W5D4.

Entry information

Entry namePOMT2_DROME
AccessionPrimary (citable) accession number: Q9W5D4
Secondary accession number(s): O77437
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase