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Protein

Protein O-mannosyl-transferase 2

Gene

tw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Rt/POMT1 and tw/POMT2 function as a protein O-mannosyltransferase in association with each other to generate and maintain normal muscle development.2 Publications

Catalytic activityi

Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  • ER-associated misfolded protein catabolic process Source: GO_Central
  • lipid glycosylation Source: UniProtKB
  • muscle attachment Source: FlyBase
  • muscle organ development Source: UniProtKB
  • protein O-linked mannosylation Source: FlyBase
  • sarcomere organization Source: FlyBase
  • somatic muscle development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-DME-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Short name:
DmPOMT2
Short name:
dPOMT2
Protein twisted
Gene namesi
Name:tw
Synonyms:Pomt2
ORF Names:CG12311
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0086368. tw.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Transmembranei566 – 586HelicalSequence analysisAdd BLAST21
Transmembranei667 – 687HelicalSequence analysisAdd BLAST21
Transmembranei689 – 709HelicalSequence analysisAdd BLAST21
Transmembranei719 – 739HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: GO_Central
  • integral component of membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Death during development, the few adult escapers exhibit clockwise rotation of the abdomen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214901 – 765Protein O-mannosyl-transferase 2Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi445N-linked (GlcNAc...)Sequence analysis1
Glycosylationi751N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9W5D4.
PRIDEiQ9W5D4.

Expressioni

Tissue specificityi

At the cellular blastoderm stage, expression accumulates in the ventrally located mesoderm primordium. At germ band extension, mesoderm expression is seen as stripes of strong expression. A very strong signal is also detected in the invaginating gut. As the germ band retracts, mesodermal expression decays and becomes restricted to somatic muscle precursors.1 Publication

Developmental stagei

Expressed throughout development; expression peaks at embryonic stages 8-16.2 Publications

Gene expression databases

BgeeiFBgn0086368.
GenevisibleiQ9W5D4. DM.

Interactioni

Subunit structurei

Interacts with Rt/POMT1.Curated

Protein-protein interaction databases

STRINGi7227.FBpp0070134.

Structurei

3D structure databases

ProteinModelPortaliQ9W5D4.
SMRiQ9W5D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 374MIR 1PROSITE-ProRule annotationAdd BLAST57
Domaini384 – 440MIR 2PROSITE-ProRule annotationAdd BLAST57
Domaini445 – 501MIR 3PROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the glycosyltransferase 39 family.Sequence analysis
Contains 3 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3359. Eukaryota.
COG1928. LUCA.
GeneTreeiENSGT00860000133869.
InParanoidiQ9W5D4.
KOiK00728.
OMAiTIEDLWE.
OrthoDBiEOG091G02QX.
PhylomeDBiQ9W5D4.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 2 hits.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W5D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVVKTPK CPRRGSVKDV AQNAPRTAPT SSKEANWNWW LLLATVFLVT
60 70 80 90 100
FATRFYKVTE PDHICWDETH FGKMGSWYIN RTFFFDVHPP LGKMLIGLSG
110 120 130 140 150
YLTGYNGTFP FEKPGDKYNE TRYQGMRYFC TTLGALIMPM GFDTVYDLTR
160 170 180 190 200
SHEAALLAAA YLIFDVGLLT LNQYILLDPI LLFFMMASVW GMVKVSKSTA
210 220 230 240 250
SGGSYGLRWW LWLFLTGTML SCTISVKFVG LFVVLLVGLH TATELWLILG
260 270 280 290 300
DLGQPILETV KQLACRAITL IVWPVLLYIL FFYIHLSVLN RSGNGDGFYS
310 320 330 340 350
SAFQSRLIGN SLYNASMPRD VAYGSLVTIK NHKTGGGYLH SHHHLYPKGS
360 370 380 390 400
GARQQQVTTY THKDENNKWL IRPHNKPGPP KGKVQILRHG DLVRLTHMAT
410 420 430 440 450
RRNLHSHNEP APMTKKHLQV TGYGELGLGD ANDVWRVLIV GGKVNETVHT
460 470 480 490 500
VTSRLKFIHL LQNCALTSSG KQLPKWGFEQ QEVSCNPNVR DKNSQWNVED
510 520 530 540 550
NEHKLMPSVS FSVYAPGFFA RFLESHAVML QGNAGLKPKE GEVTSRPWQW
560 570 580 590 600
PINYRGQFFS GSSYRIYLLG NPLIWWSNLV FLALFVTVFL CNAVVQQRRA
610 620 630 640 650
GFARSAAQNQ AQVPDSETVA QDEESEHSTT DICSCCTPAK EIVPKAVPSG
660 670 680 690 700
SPEAPNPAQS LRAAAWLFLG WMLHYLPFWA MGRVLYFHHY FPALIFNSLL
710 720 730 740 750
TGVMYNYILR VLPKWIHHVI LGLVLSILVY SFAAFSPLAY GMSGPLANEP
760
NSTMYNLKWL STWEF
Length:765
Mass (Da):86,250
Last modified:May 1, 2000 - v1
Checksum:i250CC8486330CB94
GO

Sequence cautioni

The sequence CAA20897 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59T → GS in allele tw[1]. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB176551 mRNA. Translation: BAD54755.1.
AE014298 Genomic DNA. Translation: AAF45548.1.
AL031583 Genomic DNA. Translation: CAA20897.1. Sequence problems.
AY095525 mRNA. Translation: AAM12256.1.
PIRiT13483.
RefSeqiNP_001259102.1. NM_001272173.1.
NP_569858.1. NM_130502.3.
UniGeneiDm.12266.

Genome annotation databases

EnsemblMetazoaiFBtr0070139; FBpp0070134; FBgn0086368.
FBtr0335403; FBpp0307386; FBgn0086368.
GeneIDi31024.
KEGGidme:Dmel_CG12311.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB176551 mRNA. Translation: BAD54755.1.
AE014298 Genomic DNA. Translation: AAF45548.1.
AL031583 Genomic DNA. Translation: CAA20897.1. Sequence problems.
AY095525 mRNA. Translation: AAM12256.1.
PIRiT13483.
RefSeqiNP_001259102.1. NM_001272173.1.
NP_569858.1. NM_130502.3.
UniGeneiDm.12266.

3D structure databases

ProteinModelPortaliQ9W5D4.
SMRiQ9W5D4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0070134.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Proteomic databases

PaxDbiQ9W5D4.
PRIDEiQ9W5D4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070139; FBpp0070134; FBgn0086368.
FBtr0335403; FBpp0307386; FBgn0086368.
GeneIDi31024.
KEGGidme:Dmel_CG12311.

Organism-specific databases

CTDi8168.
FlyBaseiFBgn0086368. tw.

Phylogenomic databases

eggNOGiKOG3359. Eukaryota.
COG1928. LUCA.
GeneTreeiENSGT00860000133869.
InParanoidiQ9W5D4.
KOiK00728.
OMAiTIEDLWE.
OrthoDBiEOG091G02QX.
PhylomeDBiQ9W5D4.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-DME-5173105. O-linked glycosylation.

Miscellaneous databases

GenomeRNAii31024.
PROiQ9W5D4.

Gene expression databases

BgeeiFBgn0086368.
GenevisibleiQ9W5D4. DM.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 2 hits.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOMT2_DROME
AccessioniPrimary (citable) accession number: Q9W5D4
Secondary accession number(s): O77437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.