Q9W568 (HFW1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein halfway

Alternative name(s):
Protein singed wings

Gene names

Name:	hfw
Synonyms:	swi
ORF Names:	CG3095

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in the ecdysone induced cascade; probably indirect control of 'late' ecdysone genes. Ref.1
Disruption phenotype	Mutants specifically interrupt the transmission of the regulatory signal from early to late ecdysone inducible genes. Ref.1
Sequence similarities	Contains 7 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Domain	Leucine-rich repeat Repeat Signal
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from mutant phenotype Ref.1. Source: UniProtKB pupariation Inferred from mutant phenotype Ref.1. Source: UniProtKB response to ecdysone Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 611	589	Protein halfway		PRO_0000021413
Regions
	Repeat	236 – 257	22	LRR 1
	Repeat	259 – 280	22	LRR 2
	Repeat	283 – 304	22	LRR 3
	Repeat	313 – 334	22	LRR 4
	Domain	361 – 416	56	LRRNT
	Repeat	417 – 438	22	LRR 5
	Repeat	443 – 464	22	LRR 6
	Repeat	468 – 489	22	LRR 7
	Domain	505 – 554	50	LRRCT
	Compositional bias	42 – 58	17	His-rich
	Compositional bias	76 – 121	46	Thr-rich
Amino acid modifications
	Glycosylation	221	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	246	1	N-linked (GlcNAc...) Ref.6 Ref.7
	Glycosylation	264	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	268	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	269	1	N-linked (GlcNAc...) Ref.7
Experimental info
	Mutagenesis	379	1	C → Y in swi-t467; lethal during puparium formation. Ref.1
	Sequence conflict	84	1	E → K in AAL48553. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9W568 [UniParc]. Last modified October 1, 2002. Version 3. Checksum: E8FE83F1F00DFB13	FASTA	611	68,545
	10 20 30 40 50 60 MLAYTHGTWL LLLLLLVAGA CARPEIAHTP DPAALGEEST PHAHAHPQAR HHHHAHPHAP 70 80 90 100 110 120 LKEDEQGTAI PAPILTTDNV GIGETTTASS LAETQSMSDP GSVTDTTSTS TSHSTSTTST 130 140 150 160 170 180 TSPAPLPPAA PEQPEYLKHC FYAEEQLCGH TFDGRAETSE GQGSTVAQSE AQNRGGQGNS 190 200 210 220 230 240 QCQCREHPTR PNSWYCCNIS QLTMISSCSN ISKWTNLHVR NMTVEDMDLS NPIFRSLQSL 250 260 270 280 290 300 AVTDGNITRL VNAFPRLSAL KCLNISNNNI SEIHSRAVKD VPHLEFFGMS NNNLSLVPHR 310 320 330 340 350 360 NQNKNITLDI SGNMRMLCTP LNEIIYTESI NFLNPKHSYC QYNATHTWFQ STDKVSVEQL 370 380 390 400 410 420 ENRKRCVTNC PVIPNYGSCN CTLENIMIIQ DNQSKPQCHV DCSNLGLVEL PQRLPDNTFM 430 440 450 460 470 480 LNITNNKITS LGDYFHTNPT YHNINRLLAD NNQISSIYEF EGTKFIETFQ RIYMRNNSLS 490 500 510 520 530 540 KIPEYFLNNA LMDSGLGRRI YLAGNKLQCD CNSAKTLQNW LKERSSDIPD YMEIRCRNMP 550 560 570 580 590 600 QRVIELQEAK LCQSPPDWTD YIYYLIAAEV LLLLALITKV SYDYWVFKTA GYLPWPASKM 610 PKLPCDWLCE S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the singed wings locus of Drosophila melanogaster." Schwartz Y.B., Boykova T., Belyaeva E.S., Ashburner M., Zhimulev I.F. BMC Genet. 5:15-15(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF CYS-379, DISRUPTION PHENOTYPE. Strain: y swi w.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Oregon-R.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-221 AND ASN-246, MASS SPECTROMETRY. Strain: Oregon-R. Tissue: Head.
[7]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246; ASN-264; ASN-268 AND ASN-269, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ626646 Genomic DNA. Translation: CAF25166.1. AE014298 Genomic DNA. Translation: AAF45654.3. AL021726 Genomic DNA. Translation: CAA16810.1. AY070931 mRNA. Translation: AAL48553.1.
RefSeq	NP_001259135.1. NM_001272206.1. NP_569933.2. NM_130577.4.
UniGene	Dm.5411.
3D structure databases
ProteinModelPortal	Q9W568.
SMR	Q9W568. Positions 208-301, 397-484.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1023607.
Proteomic databases
PaxDb	Q9W568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0070270; FBpp0070260; FBgn0001189. FBtr0333115; FBpp0305326; FBgn0001189.
GeneID	31120.
KEGG	dme:Dmel_CG3095.
Organism-specific databases
CTD	31120.
FlyBase	FBgn0001189. hfw.
Phylogenomic databases
eggNOG	NOG289130.
GeneTree	ENSGT00530000065147.
HOGENOM	HOG000264157.
InParanoid	Q9W568.
OMA	TAGYLPW.
OrthoDB	EOG4NS1SS.
PhylomeDB	Q9W568.
Gene expression databases
Bgee	Q9W568.
GermOnline	CG3095. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	SMARCA1. drosophila.
GenomeRNAi	31120.
NextBio	772017.

Entry information

Entry name

HFW1_DROME

Accession

Primary (citable) accession number: Q9W568
Secondary accession number(s): O46061, Q8SZE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project