ID   Q9W541_DROME            Unreviewed;       669 AA.
AC   Q9W541;
DT   01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 195.
DE   RecName: Full=Translation initiation factor eIF2B subunit epsilon {ECO:0000256|ARBA:ARBA00044144};
DE   AltName: Full=eIF2B GDP-GTP exchange factor subunit epsilon {ECO:0000256|ARBA:ARBA00044345};
GN   Name=eIF2Bepsilon {ECO:0000313|EMBL:AAF45695.1,
GN   ECO:0000313|FlyBase:FBgn0023512};
GN   Synonyms=Dmel\CG3806 {ECO:0000313|EMBL:AAF45695.1}, EG:86E4.1
GN   {ECO:0000313|EMBL:AAF45695.1}, eIF-2b(epsilon)
GN   {ECO:0000313|EMBL:AAM11381.1}, eIF-2bepsilon
GN   {ECO:0000313|EMBL:AAF45695.1}, eIF2B-epsilon
GN   {ECO:0000313|EMBL:AAF45695.1}, elf2B-epsilon
GN   {ECO:0000313|EMBL:AAF45695.1};
GN   ORFNames=CG3806 {ECO:0000313|EMBL:AAF45695.1,
GN   ECO:0000313|FlyBase:FBgn0023512}, Dmel_CG3806
GN   {ECO:0000313|EMBL:AAF45695.1};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803};
RN   [1] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA   An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA   Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA   Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA   Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA   Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA   Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA   Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2] {ECO:0000313|EMBL:CAC82995.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Pomar N.;
RL   Thesis (2001), Department of Facultad de Ciencias, Universidad Autonoma de
RL   Madrid, Madrid, Spain.
RN   [3] {ECO:0000313|EMBL:CAC82995.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Pomar N., de Haro C.;
RT   "Characterization of the Drosophila melanogaster eIF2B.";
RL   Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=12537568;
RA   Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA   Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA   George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA   Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA   Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA   Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT   "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT   euchromatic genome sequence.";
RL   Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN   [5] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [6] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=12537573;
RA   Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA   Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA   Celniker S.E.;
RT   "The transposable elements of the Drosophila melanogaster euchromatin: a
RT   genomics perspective.";
RL   Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN   [7] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=12537574;
RA   Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA   Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA   Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA   Karpen G.H.;
RT   "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL   Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN   [8] {ECO:0000313|EMBL:AAM11381.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Berkeley {ECO:0000313|EMBL:AAM11381.1};
RA   Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA   Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA   Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA   Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA   Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL   Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [9] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA   Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA   Ashburner M., Anxolabehere D.;
RT   "Combined evidence annotation of transposable elements in genome
RT   sequences.";
RL   PLoS Comput. Biol. 1:166-175(2005).
RN   [10] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S.,
RA   Svirskas R., Rubin G.;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN   [11] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=17569856; DOI=10.1126/science.1139815;
RA   Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT   "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL   Science 316:1586-1591(2007).
RN   [12] {ECO:0000313|EMBL:AAF45695.1, ECO:0000313|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX   PubMed=17569867; DOI=10.1126/science.1139816;
RA   Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA   Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA   Dimitri P., Karpen G.H., Celniker S.E.;
RT   "Sequence finishing and mapping of Drosophila melanogaster
RT   heterochromatin.";
RL   Science 316:1625-1628(2007).
RN   [13] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=26109357; DOI=.1534/g3.115.018929;
RG   FlyBase Consortium;
RA   Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E.,
RA   Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M.,
RA   Gelbart W.M., null;
RT   "Gene Model Annotations for Drosophila melanogaster: Impact of High-
RT   Throughput Data.";
RL   G3 (Bethesda) 5:1721-1736(2015).
RN   [14] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=26109356; DOI=.1534/g3.115.018937;
RG   FlyBase Consortium;
RA   Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E.,
RA   Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M.,
RA   null;
RT   "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.";
RL   G3 (Bethesda) 5:1737-1749(2015).
RN   [15] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=25589440;
RA   Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E.,
RA   Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M.,
RA   Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M.,
RA   de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M.,
RA   Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M.,
RA   Karpen G.H., Celniker S.E.;
RT   "The Release 6 reference sequence of the Drosophila melanogaster genome.";
RL   Genome Res. 25:445-458(2015).
RN   [16] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RG   Berkeley Drosophila Genome Project;
RA   Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA   Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA   Rubin G.;
RT   "Drosophila melanogaster release 4 sequence.";
RL   Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
RN   [17] {ECO:0000313|EMBL:AAF45695.1}
RP   NUCLEOTIDE SEQUENCE.
RG   FlyBase;
RL   Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000256|ARBA:ARBA00004514}.
CC   -!- SIMILARITY: Belongs to the eIF-2B gamma/epsilon subunits family.
CC       {ECO:0000256|ARBA:ARBA00007878}.
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DR   EMBL; AE014298; AAF45695.1; -; Genomic_DNA.
DR   EMBL; AY095053; AAM11381.1; -; mRNA.
DR   EMBL; AE014298; AAS65247.1; -; Genomic_DNA.
DR   EMBL; AJ344150; CAC82995.1; -; mRNA.
DR   RefSeq; NP_569961.2; NM_130605.5.
DR   RefSeq; NP_996329.1; NM_206606.3.
DR   AlphaFoldDB; Q9W541; -.
DR   SMR; Q9W541; -.
DR   IntAct; Q9W541; 2.
DR   MINT; Q9W541; -.
DR   STRING; 7227.FBpp0089217; -.
DR   PaxDb; 7227-FBpp0089217; -.
DR   EnsemblMetazoa; FBtr0070350; FBpp0070336; FBgn0023512.
DR   EnsemblMetazoa; FBtr0070351; FBpp0089217; FBgn0023512.
DR   GeneID; 31156; -.
DR   KEGG; dme:Dmel_CG3806; -.
DR   UCSC; CG3806-RA; d. melanogaster.
DR   AGR; FB:FBgn0023512; -.
DR   CTD; 31156; -.
DR   FlyBase; FBgn0023512; eIF2Bepsilon.
DR   VEuPathDB; VectorBase:FBgn0023512; -.
DR   eggNOG; KOG1461; Eukaryota.
DR   GeneTree; ENSGT00510000047568; -.
DR   HOGENOM; CLU_012507_2_0_1; -.
DR   InParanoid; Q9W541; -.
DR   OMA; LAQSCKI; -.
DR   OrthoDB; 5474157at2759; -.
DR   Reactome; R-DME-72731; Recycling of eIF2:GDP.
DR   BioGRID-ORCS; 31156; 0 hits in 1 CRISPR screen.
DR   ChiTaRS; eIF2B-epsilon; fly.
DR   GenomeRNAi; 31156; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0023512; Expressed in cleaving embryo and 22 other cell types or tissues.
DR   ExpressionAtlas; Q9W541; baseline and differential.
DR   GO; GO:0005829; C:cytosol; ISS:FlyBase.
DR   GO; GO:0005851; C:eukaryotic translation initiation factor 2B complex; ISS:FlyBase.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:FlyBase.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003743; F:translation initiation factor activity; ISS:FlyBase.
DR   GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR   GO; GO:0006413; P:translational initiation; ISS:FlyBase.
DR   CDD; cd04197; eIF-2B_epsilon_N; 1.
DR   CDD; cd11558; W2_eIF2B_epsilon; 1.
DR   Gene3D; 1.25.40.180; -; 1.
DR   Gene3D; 2.160.10.10; Hexapeptide repeat proteins; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR035543; eIF-2B_epsilon_N.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR003307; W2_domain.
DR   InterPro; IPR044123; W2_eIF2B_epsilon.
DR   PANTHER; PTHR45887; TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON; 1.
DR   PANTHER; PTHR45887:SF1; TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   Pfam; PF02020; W2; 1.
DR   SMART; SM00515; eIF5C; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR   PROSITE; PS51363; W2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Initiation factor {ECO:0000313|EMBL:AAF45695.1};
KW   Nucleotidyltransferase {ECO:0000313|EMBL:AAF45695.1};
KW   Protein biosynthesis {ECO:0000313|EMBL:AAF45695.1};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:Q9W541};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000803};
KW   Transferase {ECO:0000313|EMBL:AAF45695.1}.
FT   DOMAIN          498..665
FT                   /note="W2"
FT                   /evidence="ECO:0000259|PROSITE:PS51363"
SQ   SEQUENCE   669 AA;  75019 MW;  C25B1D7A1A516BA0 CRC64;
     MAQFEKEIVQ AVLIADNNVW NFKPLSDEGS TALLPLVNVR MLDYALMALN RSGVEEVFVY
     TSLYRSSIRE HIRAGIATDA AWSFKMTVHV IGGEGCRCFG DAMRDLDNKA LIRGHFILLG
     ADTVTNADLR PVLEQHKRQA KFDKGTAATL VFKECANNVR TGNEVLIAVD RRNDRLHYHQ
     RLRMHHKENR YQLPLDVFLG NSCVALHHNL LDPQIAIGSP SMLSLFNDNF DFQTRDDFVR
     GLLINEELLD SRIYVALLPA AQYAHKVNNW PAYQLVSRDI INRWAYPFVP DIGLYKLQQE
     YVFHKDNIYK SPEAHVSKVA LLQNVVIEAG SHVDSGSVIS DSVIGANCRI GKNCRLTNAF
     LMADVTVMDN CRLEHCVVGE GAIINEDCDV SAGCVLGAKS VLPAKKTLAK TLITRTPSRR
     RSDDEVVVEL KSIGPHAYIV SDLTTGDPED SECEDLLPQQ PLSIPKMGDL LAPLDEISYC
     SDFSEDDEDA SRAVTPLPDD TNIFLGEVID SLTRGFREKS NPDFLVLEIN SSRYAYNMSL
     KEVNFNVVKA VFGMQSIVEP ANDNVLVAIN AAFKQLGPVV SNYIKSEDSM MDCLKALEDV
     YEENHLVRGK ISQIVHYLYD KDFVSESAIQ AWYAQLDEEE HAHLRQSLAK LVAWLDQSSE
     EDDDEEDDD
//