Reviewed, UniProtKB/Swiss-Prot Q9W539 (HR4_DROME)

Names and origin

Protein names

Recommended name:
    Hormone receptor 4
Alternative name(s):
    dHR4
    Nuclear receptor subfamily 6 group A member 2

Gene names

Name:	Hr4
Synonyms:	NR6A2
ORF Names:	CG16902

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Coordinates growth and maturation by mediating endocrine responses to the attainment of critical weight during larval development. Plays a central role in the genetic cascades triggered by the steroid hormone ecdysone at the onset of metamorphosis, acting as both a repressor of the early ecdysone-induced regulatory genes and an inducer of the ftz-f1 midprepupal competence factor. Ref.1
Subcellular location	Nucleus. Ref.1
Tissue specificity	During L2 and L3 stages, strong constitutive expression is seen in the ring gland. Lower expression is detected in specific neurons of the central nervous system (CNS) (at protein level). Ref.1
Disruption phenotype	Flies exhibit larvae that precociously leave the food to form premature prepupae, resulting in abbreviated larval development that translates directly into smaller and lighter animals. Ref.1
Sequence similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Sequence caution	The sequence CAA22836.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	growth Ref.1 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW steroid hormone receptor signaling pathway Ref.1 Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1518	1518	Hormone receptor 4		PRO_0000053522
Regions
	DNA binding	918 – 993	76	Nuclear receptor
	Zinc finger	921 – 941	21	NR C4-type
	Zinc finger	957 – 976	20	NR C4-type
	Compositional bias	94 – 144	51	Ala-rich
	Compositional bias	135 – 223	89	Ser-rich
	Compositional bias	239 – 277	39	Gly-rich
	Compositional bias	401 – 427	27	Arg-rich
	Compositional bias	460 – 1194	735	Gln-rich
Experimental info
	Sequence conflict	140	1	T → R in CAA22836. Ref.1
	Sequence conflict	144	1	A → R in CAA22836. Ref.1
	Sequence conflict	213	1	I → V in CAA22836. Ref.1
	Sequence conflict	1452	1	K → KA in CAA22836. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9W539-1 [UniParc]. Last modified May 10, 2005. Version 4. Checksum: DDBDFEBA416D17AA	FASTA	1,518	162,655
	10 20 30 40 50 60 MTLSRGPYSE LDKMSLFQDL KLKRRKIDSR CSSDGESIAD TSTSSPDLLA PMSPKLCDSG 70 80 90 100 110 120 SAGASLGASL PLPLALPLPM ALPLPMSLPL PLTAASSAVT VSLAAVVAAV AETGGAGAGG 130 140 150 160 170 180 AGTAVTASGA GPCVSTSSTT AAAATSSTSS LSSSSSSSSS TSSSTSSASP TAGASSTATC 190 200 210 220 230 240 PASSSSSSGN GSGGKSGSIK QEHTEIHSSS SAISAAAAST VMSPPPAEAT RSSPATPEGG 250 260 270 280 290 300 GPAGDGSGAT GGGNTSGGST AGVAINEHQN NGNGSGGSSR ASPDSLEEKP STTTTTGRPT 310 320 330 340 350 360 LTPTNGVLSS ASAGTGISTG SSAKLSEAGM SVIRSVKEER LLNVSSKMLV FHQQREQETK 370 380 390 400 410 420 AVAAAAAAAA AGHVTVLVTP SRIKSEPPPP ASPSSTSSTQ RERERERDRE RDRERERERD 430 440 450 460 470 480 RDREREREQS ISSSQQHLSR VSASPPTQLS HGSLGPNIVQ THHLHQQLTQ PLTLRKSSPP 490 500 510 520 530 540 TEHLLSQSMQ HLTQQQAIHL HHLLGQQQQQ QQASHPQQQQ QQQHSPHSLV RVKKEPNVGQ 550 560 570 580 590 600 RHLSPHHQQQ SPLLQHHQQQ QQQQQQQQQH LHQQQQQQQH HQQQPQALAL MHPASLALRN 610 620 630 640 650 660 SNRDAAILFR VKSEVHQQVA AGLPHLMQSA GGAAAAAAAA VAAQRMVCFS NARINGVKPE 670 680 690 700 710 720 VIGGPLGNLR PVGVGGGNGS GSVQCPSPHP SSSSSSSQLS PQTPSQTPPR GTPTVIMGES 730 740 750 760 770 780 CGVRTMVWGY EPPPPSAGQS HGQHPQQQQQ SPHHQPQQQQ QQQQQQSQQQ QQQQQQQSLG 790 800 810 820 830 840 QQQHCLSSPS AGSLTPSSSS GGGSVSGGGV GGPLTPSSVA PQNNEEAAQL LLSLGQTRIQ 850 860 870 880 890 900 DMRSRPHPFR TPHALNMERL WAGDYSQLPP GQLQALNLSA QQQQWGSSNS TGLGGVGGGM 910 920 930 940 950 960 GGRNLEAPHE PTDEDEQPLV CMICEDKATG LHYGIITCEG CKGFFKRTVQ NRRVYTCVAD 970 980 990 1000 1010 1020 GTCEITKAQR NRCQYCRFKK CIEQGMVLQA VREDRMPGGR NSGAVYNLYK VKYKKHKKTN 1030 1040 1050 1060 1070 1080 QKQQQQAAQQ QQQQAAAQQQ HQQQQQHQQH QQHQQQQLHS PLHHHHHQGH QSHHAQQQHH 1090 1100 1110 1120 1130 1140 PQLSPHHLLS PQQQQLAAAV AAAAQHQQQQ QQQQQQQQQA KLMGGVVDMK PMFLGPALKP 1150 1160 1170 1180 1190 1200 ELLQAPPMHS PAQQQQQQQQ QQQQQQASPH LSLSSPHQQQ QQQQGQHQNH HQQQGGGGGG 1210 1220 1230 1240 1250 1260 AGGGAQLPPH LVNGTILKTA LTNPSEIVHL RHRLDSAVSS SKDRQISYEH ALGMIQTLID 1270 1280 1290 1300 1310 1320 CDAMEDIATL PHFSEFLEDK SEISEKLCNI GDSIVHKLVS WTKKLPFYLE IPVEIHTKLL 1330 1340 1350 1360 1370 1380 TDKWHEILIL TTAAYQALHG KRRGEGGGSR HGSPASTPLS TPTGTPLSTP IPSPAQPLHK 1390 1400 1410 1420 1430 1440 DDPEFVSEVN SHLSTLQTCL TTLMGQPIAM EQLKLDVGHM VDKMTQITIM FRRIKLKMEE 1450 1460 1470 1480 1490 1500 YVCLKVYILL NKEVELESIQ ERYVQVLRSY LQNSSPQNPQ ARLSELLSHI PEIQAAASLL 1510 LESKMFYVPF VLNSASIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ecdysone-induced DHR4 orphan nuclear receptor coordinates growth and maturation in Drosophila." King-Jones K., Charles J.P., Lam G., Thummel C.S. Cell 121:773-784(2005) [PubMed: 15935763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed: 10731137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Oregon-R.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY971884 mRNA. Translation: AAX73355.1. AE014298 Genomic DNA. Translation: ABC67166.1. AL035245 Genomic DNA. Translation: CAA22836.1. Sequence problems.
RefSeq	NP_001033823.1.
UniGene	Dm.26541
3D structure databases
HSSP	HSSP built from PDB template 2NLL based on UniProtKB P19793.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9W539. 1 interaction.
Genome annotation databases
Ensembl	FBgn0023546. Drosophila melanogaster. [Contig view]
GeneID	31162.
KEGG	dme:Dmel_CG16902.
Organism-specific databases
FlyBase	FBgn0023546. Hr4.
Phylogenomic databases
HOGENOM	Q9W539.
Gene expression databases
ArrayExpress	Q9W539.
GermOnline	CG16902. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR001723. Str_hrmn_rcpt. IPR000324. VitD_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:1.10.565.10. Nucl_hrmn_rcpt_lig_bd. 1 hit. G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00398. STRDHORMONER. PR00047. STROIDFINGER. PR00350. VITAMINDR.
ProDom	PD000035. Znf_C4steroid. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	772237.

Entry information

Entry name

HR4_DROME

Accession

Primary (citable) accession number: Q9W539
Secondary accession number(s): O96836, Q56AW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	June 16, 2009
This is version 67 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project